Amino acid dipepetide frequency for Staphylococcus phage SA537ruMSSAST97

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.79AlaAla: 1.79 ± 0.676
0.389AlaCys: 0.389 ± 0.189
2.335AlaAsp: 2.335 ± 0.446
2.957AlaGlu: 2.957 ± 0.536
2.257AlaPhe: 2.257 ± 0.513
3.424AlaGly: 3.424 ± 0.542
0.311AlaHis: 0.311 ± 0.233
4.436AlaIle: 4.436 ± 0.758
4.436AlaLys: 4.436 ± 0.509
4.747AlaLeu: 4.747 ± 0.805
1.712AlaMet: 1.712 ± 0.365
3.969AlaAsn: 3.969 ± 0.579
1.401AlaPro: 1.401 ± 0.311
2.957AlaGln: 2.957 ± 0.545
3.113AlaArg: 3.113 ± 0.516
4.358AlaSer: 4.358 ± 0.522
3.269AlaThr: 3.269 ± 0.579
3.269AlaVal: 3.269 ± 0.549
0.623AlaTrp: 0.623 ± 0.234
2.568AlaTyr: 2.568 ± 0.464
0.0AlaXaa: 0.0 ± 0.0
Cys
0.233CysAla: 0.233 ± 0.123
0.078CysCys: 0.078 ± 0.074
0.233CysAsp: 0.233 ± 0.17
0.233CysGlu: 0.233 ± 0.138
0.311CysPhe: 0.311 ± 0.149
0.311CysGly: 0.311 ± 0.189
0.156CysHis: 0.156 ± 0.101
0.856CysIle: 0.856 ± 0.253
0.389CysLys: 0.389 ± 0.197
0.389CysLeu: 0.389 ± 0.154
0.0CysMet: 0.0 ± 0.0
0.467CysAsn: 0.467 ± 0.203
0.0CysPro: 0.0 ± 0.0
0.156CysGln: 0.156 ± 0.109
0.311CysArg: 0.311 ± 0.174
0.389CysSer: 0.389 ± 0.159
0.389CysThr: 0.389 ± 0.142
0.233CysVal: 0.233 ± 0.13
0.0CysTrp: 0.0 ± 0.0
0.467CysTyr: 0.467 ± 0.204
0.0CysXaa: 0.0 ± 0.0
Asp
2.802AspAla: 2.802 ± 0.418
0.467AspCys: 0.467 ± 0.177
4.28AspAsp: 4.28 ± 0.719
5.604AspGlu: 5.604 ± 0.905
3.191AspPhe: 3.191 ± 0.538
4.436AspGly: 4.436 ± 0.564
0.934AspHis: 0.934 ± 0.243
4.514AspIle: 4.514 ± 0.569
5.448AspLys: 5.448 ± 0.58
5.214AspLeu: 5.214 ± 0.675
1.245AspMet: 1.245 ± 0.278
4.592AspAsn: 4.592 ± 0.558
1.401AspPro: 1.401 ± 0.381
1.012AspGln: 1.012 ± 0.348
2.49AspArg: 2.49 ± 0.479
3.658AspSer: 3.658 ± 0.608
3.736AspThr: 3.736 ± 0.567
3.969AspVal: 3.969 ± 0.645
0.389AspTrp: 0.389 ± 0.185
3.347AspTyr: 3.347 ± 0.534
0.0AspXaa: 0.0 ± 0.0
Glu
4.514GluAla: 4.514 ± 0.69
0.233GluCys: 0.233 ± 0.122
3.502GluAsp: 3.502 ± 0.502
6.693GluGlu: 6.693 ± 0.838
3.658GluPhe: 3.658 ± 0.613
2.49GluGly: 2.49 ± 0.393
1.167GluHis: 1.167 ± 0.288
6.849GluIle: 6.849 ± 0.895
6.382GluLys: 6.382 ± 0.796
8.25GluLeu: 8.25 ± 1.194
2.646GluMet: 2.646 ± 0.438
5.37GluAsn: 5.37 ± 0.609
1.946GluPro: 1.946 ± 0.33
4.047GluGln: 4.047 ± 0.584
3.969GluArg: 3.969 ± 0.66
4.436GluSer: 4.436 ± 0.577
3.736GluThr: 3.736 ± 0.489
4.592GluVal: 4.592 ± 0.658
0.7GluTrp: 0.7 ± 0.231
4.047GluTyr: 4.047 ± 0.575
0.0GluXaa: 0.0 ± 0.0
Phe
1.946PheAla: 1.946 ± 0.377
0.156PheCys: 0.156 ± 0.112
3.113PheAsp: 3.113 ± 0.46
3.269PheGlu: 3.269 ± 0.563
1.167PhePhe: 1.167 ± 0.349
2.49PheGly: 2.49 ± 0.436
0.934PheHis: 0.934 ± 0.276
3.58PheIle: 3.58 ± 0.707
4.903PheLys: 4.903 ± 0.556
2.646PheLeu: 2.646 ± 0.449
1.245PheMet: 1.245 ± 0.353
4.436PheAsn: 4.436 ± 0.664
0.623PhePro: 0.623 ± 0.226
0.856PheGln: 0.856 ± 0.243
1.323PheArg: 1.323 ± 0.35
2.179PheSer: 2.179 ± 0.356
2.179PheThr: 2.179 ± 0.437
2.179PheVal: 2.179 ± 0.438
0.156PheTrp: 0.156 ± 0.117
1.479PheTyr: 1.479 ± 0.297
0.0PheXaa: 0.0 ± 0.0
Gly
3.269GlyAla: 3.269 ± 0.677
0.311GlyCys: 0.311 ± 0.142
2.724GlyAsp: 2.724 ± 0.569
2.568GlyGlu: 2.568 ± 0.514
2.49GlyPhe: 2.49 ± 0.57
2.802GlyGly: 2.802 ± 0.842
1.012GlyHis: 1.012 ± 0.25
4.047GlyIle: 4.047 ± 0.712
5.526GlyLys: 5.526 ± 0.843
5.604GlyLeu: 5.604 ± 0.829
1.245GlyMet: 1.245 ± 0.392
3.424GlyAsn: 3.424 ± 0.563
0.7GlyPro: 0.7 ± 0.28
2.335GlyGln: 2.335 ± 0.442
2.88GlyArg: 2.88 ± 0.482
3.035GlySer: 3.035 ± 0.39
2.802GlyThr: 2.802 ± 0.437
3.035GlyVal: 3.035 ± 0.618
0.934GlyTrp: 0.934 ± 0.265
2.88GlyTyr: 2.88 ± 0.52
0.0GlyXaa: 0.0 ± 0.0
His
0.623HisAla: 0.623 ± 0.215
0.156HisCys: 0.156 ± 0.116
0.934HisAsp: 0.934 ± 0.263
1.634HisGlu: 1.634 ± 0.345
1.012HisPhe: 1.012 ± 0.287
0.778HisGly: 0.778 ± 0.262
0.156HisHis: 0.156 ± 0.126
1.557HisIle: 1.557 ± 0.403
1.245HisLys: 1.245 ± 0.276
1.323HisLeu: 1.323 ± 0.295
0.467HisMet: 0.467 ± 0.199
1.167HisAsn: 1.167 ± 0.299
0.389HisPro: 0.389 ± 0.189
0.7HisGln: 0.7 ± 0.293
0.7HisArg: 0.7 ± 0.192
0.934HisSer: 0.934 ± 0.264
0.856HisThr: 0.856 ± 0.39
1.167HisVal: 1.167 ± 0.32
0.156HisTrp: 0.156 ± 0.117
0.856HisTyr: 0.856 ± 0.332
0.0HisXaa: 0.0 ± 0.0
Ile
4.514IleAla: 4.514 ± 0.59
0.311IleCys: 0.311 ± 0.144
5.526IleAsp: 5.526 ± 0.679
7.16IleGlu: 7.16 ± 0.921
2.957IlePhe: 2.957 ± 0.619
4.125IleGly: 4.125 ± 0.561
1.401IleHis: 1.401 ± 0.338
5.915IleIle: 5.915 ± 0.82
7.783IleLys: 7.783 ± 0.807
4.903IleLeu: 4.903 ± 0.767
1.712IleMet: 1.712 ± 0.342
6.693IleAsn: 6.693 ± 1.109
2.179IlePro: 2.179 ± 0.355
3.113IleGln: 3.113 ± 0.489
2.802IleArg: 2.802 ± 0.468
4.825IleSer: 4.825 ± 0.576
5.292IleThr: 5.292 ± 0.57
4.67IleVal: 4.67 ± 0.447
1.167IleTrp: 1.167 ± 0.494
3.113IleTyr: 3.113 ± 0.56
0.0IleXaa: 0.0 ± 0.0
Lys
5.681LysAla: 5.681 ± 0.503
0.233LysCys: 0.233 ± 0.114
5.604LysAsp: 5.604 ± 0.437
7.938LysGlu: 7.938 ± 0.88
3.969LysPhe: 3.969 ± 0.524
5.292LysGly: 5.292 ± 0.764
1.634LysHis: 1.634 ± 0.372
7.082LysIle: 7.082 ± 0.792
8.25LysLys: 8.25 ± 1.048
7.082LysLeu: 7.082 ± 0.783
2.646LysMet: 2.646 ± 0.523
4.358LysAsn: 4.358 ± 0.503
2.957LysPro: 2.957 ± 0.542
4.592LysGln: 4.592 ± 0.748
4.203LysArg: 4.203 ± 0.574
5.759LysSer: 5.759 ± 0.681
5.292LysThr: 5.292 ± 0.633
5.448LysVal: 5.448 ± 0.657
1.09LysTrp: 1.09 ± 0.382
3.891LysTyr: 3.891 ± 0.448
0.0LysXaa: 0.0 ± 0.0
Leu
4.436LeuAla: 4.436 ± 0.614
0.467LeuCys: 0.467 ± 0.174
6.304LeuAsp: 6.304 ± 0.642
7.238LeuGlu: 7.238 ± 0.764
3.424LeuPhe: 3.424 ± 0.487
3.58LeuGly: 3.58 ± 0.781
0.934LeuHis: 0.934 ± 0.25
5.681LeuIle: 5.681 ± 0.659
8.172LeuLys: 8.172 ± 0.884
6.615LeuLeu: 6.615 ± 0.794
1.634LeuMet: 1.634 ± 0.396
5.837LeuAsn: 5.837 ± 0.536
2.335LeuPro: 2.335 ± 0.353
3.424LeuGln: 3.424 ± 0.453
3.891LeuArg: 3.891 ± 0.575
5.37LeuSer: 5.37 ± 0.82
4.358LeuThr: 4.358 ± 0.596
4.28LeuVal: 4.28 ± 0.579
0.623LeuTrp: 0.623 ± 0.245
2.724LeuTyr: 2.724 ± 0.435
0.0LeuXaa: 0.0 ± 0.0
Met
1.245MetAla: 1.245 ± 0.322
0.0MetCys: 0.0 ± 0.0
1.09MetAsp: 1.09 ± 0.238
2.101MetGlu: 2.101 ± 0.505
1.012MetPhe: 1.012 ± 0.249
0.934MetGly: 0.934 ± 0.329
0.156MetHis: 0.156 ± 0.101
2.179MetIle: 2.179 ± 0.352
2.101MetLys: 2.101 ± 0.403
1.712MetLeu: 1.712 ± 0.36
1.012MetMet: 1.012 ± 0.29
1.868MetAsn: 1.868 ± 0.362
1.401MetPro: 1.401 ± 0.41
1.167MetGln: 1.167 ± 0.317
1.09MetArg: 1.09 ± 0.354
1.712MetSer: 1.712 ± 0.294
1.79MetThr: 1.79 ± 0.458
1.245MetVal: 1.245 ± 0.245
0.311MetTrp: 0.311 ± 0.15
1.012MetTyr: 1.012 ± 0.303
0.0MetXaa: 0.0 ± 0.0
Asn
4.047AsnAla: 4.047 ± 0.519
0.156AsnCys: 0.156 ± 0.099
4.825AsnAsp: 4.825 ± 0.717
6.693AsnGlu: 6.693 ± 0.966
1.946AsnPhe: 1.946 ± 0.334
4.903AsnGly: 4.903 ± 0.908
1.167AsnHis: 1.167 ± 0.278
4.592AsnIle: 4.592 ± 0.724
5.837AsnLys: 5.837 ± 0.735
5.915AsnLeu: 5.915 ± 0.771
1.245AsnMet: 1.245 ± 0.287
4.981AsnAsn: 4.981 ± 0.715
2.49AsnPro: 2.49 ± 0.341
3.502AsnGln: 3.502 ± 0.465
2.88AsnArg: 2.88 ± 0.379
2.724AsnSer: 2.724 ± 0.529
3.424AsnThr: 3.424 ± 0.449
4.203AsnVal: 4.203 ± 0.631
1.012AsnTrp: 1.012 ± 0.472
2.568AsnTyr: 2.568 ± 0.442
0.0AsnXaa: 0.0 ± 0.0
Pro
1.09ProAla: 1.09 ± 0.313
0.0ProCys: 0.0 ± 0.0
1.79ProAsp: 1.79 ± 0.487
1.245ProGlu: 1.245 ± 0.309
0.934ProPhe: 0.934 ± 0.315
1.012ProGly: 1.012 ± 0.256
0.7ProHis: 0.7 ± 0.259
2.724ProIle: 2.724 ± 0.365
2.024ProLys: 2.024 ± 0.421
2.024ProLeu: 2.024 ± 0.487
0.778ProMet: 0.778 ± 0.266
1.557ProAsn: 1.557 ± 0.4
0.856ProPro: 0.856 ± 0.283
0.623ProGln: 0.623 ± 0.228
1.09ProArg: 1.09 ± 0.316
1.479ProSer: 1.479 ± 0.393
1.634ProThr: 1.634 ± 0.337
1.401ProVal: 1.401 ± 0.281
0.156ProTrp: 0.156 ± 0.102
1.09ProTyr: 1.09 ± 0.283
0.0ProXaa: 0.0 ± 0.0
Gln
3.269GlnAla: 3.269 ± 0.37
0.389GlnCys: 0.389 ± 0.183
2.646GlnAsp: 2.646 ± 0.457
3.035GlnGlu: 3.035 ± 0.679
1.012GlnPhe: 1.012 ± 0.222
1.557GlnGly: 1.557 ± 0.367
1.012GlnHis: 1.012 ± 0.328
2.957GlnIle: 2.957 ± 0.428
3.58GlnLys: 3.58 ± 0.697
3.113GlnLeu: 3.113 ± 0.454
0.934GlnMet: 0.934 ± 0.289
2.646GlnAsn: 2.646 ± 0.454
0.623GlnPro: 0.623 ± 0.189
1.868GlnGln: 1.868 ± 0.448
1.946GlnArg: 1.946 ± 0.349
2.413GlnSer: 2.413 ± 0.474
2.568GlnThr: 2.568 ± 0.473
1.946GlnVal: 1.946 ± 0.424
0.389GlnTrp: 0.389 ± 0.199
1.868GlnTyr: 1.868 ± 0.325
0.0GlnXaa: 0.0 ± 0.0
Arg
2.49ArgAla: 2.49 ± 0.48
0.389ArgCys: 0.389 ± 0.192
2.724ArgAsp: 2.724 ± 0.445
3.891ArgGlu: 3.891 ± 0.544
1.79ArgPhe: 1.79 ± 0.379
1.868ArgGly: 1.868 ± 0.453
0.778ArgHis: 0.778 ± 0.25
3.814ArgIle: 3.814 ± 0.652
4.047ArgLys: 4.047 ± 0.587
3.58ArgLeu: 3.58 ± 0.616
0.856ArgMet: 0.856 ± 0.271
2.724ArgAsn: 2.724 ± 0.414
0.623ArgPro: 0.623 ± 0.222
1.245ArgGln: 1.245 ± 0.345
2.101ArgArg: 2.101 ± 0.458
2.179ArgSer: 2.179 ± 0.401
2.88ArgThr: 2.88 ± 0.469
2.49ArgVal: 2.49 ± 0.481
0.778ArgTrp: 0.778 ± 0.282
2.646ArgTyr: 2.646 ± 0.483
0.0ArgXaa: 0.0 ± 0.0
Ser
3.502SerAla: 3.502 ± 0.584
0.467SerCys: 0.467 ± 0.222
4.592SerAsp: 4.592 ± 0.754
4.981SerGlu: 4.981 ± 0.812
2.724SerPhe: 2.724 ± 0.532
3.424SerGly: 3.424 ± 0.647
1.712SerHis: 1.712 ± 0.348
5.993SerIle: 5.993 ± 0.572
5.448SerLys: 5.448 ± 0.876
3.891SerLeu: 3.891 ± 0.554
1.167SerMet: 1.167 ± 0.297
4.747SerAsn: 4.747 ± 0.692
0.7SerPro: 0.7 ± 0.192
2.101SerGln: 2.101 ± 0.443
2.335SerArg: 2.335 ± 0.376
3.035SerSer: 3.035 ± 0.537
3.269SerThr: 3.269 ± 0.456
2.802SerVal: 2.802 ± 0.629
0.467SerTrp: 0.467 ± 0.177
2.413SerTyr: 2.413 ± 0.543
0.0SerXaa: 0.0 ± 0.0
Thr
3.502ThrAla: 3.502 ± 0.524
0.389ThrCys: 0.389 ± 0.162
4.047ThrAsp: 4.047 ± 0.725
3.969ThrGlu: 3.969 ± 0.703
1.946ThrPhe: 1.946 ± 0.374
4.358ThrGly: 4.358 ± 0.811
1.323ThrHis: 1.323 ± 0.246
4.67ThrIle: 4.67 ± 0.565
5.526ThrLys: 5.526 ± 0.538
4.28ThrLeu: 4.28 ± 0.434
1.012ThrMet: 1.012 ± 0.274
3.035ThrAsn: 3.035 ± 0.584
1.323ThrPro: 1.323 ± 0.316
1.712ThrGln: 1.712 ± 0.394
2.88ThrArg: 2.88 ± 0.493
4.436ThrSer: 4.436 ± 0.738
3.035ThrThr: 3.035 ± 0.586
3.658ThrVal: 3.658 ± 0.527
0.856ThrTrp: 0.856 ± 0.255
2.257ThrTyr: 2.257 ± 0.449
0.0ThrXaa: 0.0 ± 0.0
Val
2.335ValAla: 2.335 ± 0.423
0.467ValCys: 0.467 ± 0.199
3.113ValAsp: 3.113 ± 0.511
3.814ValGlu: 3.814 ± 0.649
2.335ValPhe: 2.335 ± 0.513
3.969ValGly: 3.969 ± 0.799
0.545ValHis: 0.545 ± 0.209
4.047ValIle: 4.047 ± 0.457
6.304ValLys: 6.304 ± 0.585
5.292ValLeu: 5.292 ± 0.74
1.712ValMet: 1.712 ± 0.409
4.203ValAsn: 4.203 ± 0.528
1.323ValPro: 1.323 ± 0.28
1.946ValGln: 1.946 ± 0.365
1.946ValArg: 1.946 ± 0.472
3.58ValSer: 3.58 ± 0.528
4.903ValThr: 4.903 ± 0.883
4.28ValVal: 4.28 ± 0.661
0.467ValTrp: 0.467 ± 0.204
1.868ValTyr: 1.868 ± 0.403
0.0ValXaa: 0.0 ± 0.0
Trp
0.389TrpAla: 0.389 ± 0.162
0.078TrpCys: 0.078 ± 0.085
0.778TrpAsp: 0.778 ± 0.263
0.623TrpGlu: 0.623 ± 0.197
0.545TrpPhe: 0.545 ± 0.198
0.233TrpGly: 0.233 ± 0.112
0.078TrpHis: 0.078 ± 0.085
0.934TrpIle: 0.934 ± 0.274
0.778TrpLys: 0.778 ± 0.189
1.09TrpLeu: 1.09 ± 0.389
0.623TrpMet: 0.623 ± 0.205
0.856TrpAsn: 0.856 ± 0.414
0.156TrpPro: 0.156 ± 0.102
0.467TrpGln: 0.467 ± 0.168
0.467TrpArg: 0.467 ± 0.162
0.623TrpSer: 0.623 ± 0.207
0.467TrpThr: 0.467 ± 0.151
1.012TrpVal: 1.012 ± 0.31
0.156TrpTrp: 0.156 ± 0.103
0.389TrpTyr: 0.389 ± 0.2
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.646TyrAla: 2.646 ± 0.46
0.467TyrCys: 0.467 ± 0.208
2.568TyrAsp: 2.568 ± 0.433
3.58TyrGlu: 3.58 ± 0.421
2.179TyrPhe: 2.179 ± 0.388
1.634TyrGly: 1.634 ± 0.396
0.7TyrHis: 0.7 ± 0.276
3.736TyrIle: 3.736 ± 0.519
4.825TyrLys: 4.825 ± 0.516
3.736TyrLeu: 3.736 ± 0.559
1.167TyrMet: 1.167 ± 0.308
2.179TyrAsn: 2.179 ± 0.45
0.778TyrPro: 0.778 ± 0.197
1.946TyrGln: 1.946 ± 0.407
1.479TyrArg: 1.479 ± 0.344
2.646TyrSer: 2.646 ± 0.443
2.335TyrThr: 2.335 ± 0.456
2.568TyrVal: 2.568 ± 0.438
0.311TyrTrp: 0.311 ± 0.171
1.557TyrTyr: 1.557 ± 0.532
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (12850 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski