Fire ant associated circular virus 1
Average proteome isoelectric point is 6.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BPB1|A0A346BPB1_9VIRU Putative capsid protein OS=Fire ant associated circular virus 1 OX=2293280 PE=3 SV=1
MM1 pKa = 7.79 ASTKK5 pKa = 10.25 KK6 pKa = 10.46 FRR8 pKa = 11.84 FQNTSALLTYY18 pKa = 8.3 KK19 pKa = 9.25 THH21 pKa = 7.07 LDD23 pKa = 3.04 KK24 pKa = 10.96 KK25 pKa = 10.89 AYY27 pKa = 10.47 LEE29 pKa = 4.12 WFEE32 pKa = 4.61 KK33 pKa = 9.95 QAKK36 pKa = 5.67 QTPVFIRR43 pKa = 11.84 LAHH46 pKa = 5.47 EE47 pKa = 4.52 TGSHH51 pKa = 7.43 DD52 pKa = 4.45 EE53 pKa = 4.61 GEE55 pKa = 4.05 PDD57 pKa = 3.4 YY58 pKa = 11.73 DD59 pKa = 3.53 HH60 pKa = 6.32 THH62 pKa = 5.45 VVFKK66 pKa = 10.48 CKK68 pKa = 10.39 SRR70 pKa = 11.84 IDD72 pKa = 3.52 TTSQRR77 pKa = 11.84 FFDD80 pKa = 4.72 FNAIHH85 pKa = 6.06 PHH87 pKa = 5.17 IKK89 pKa = 9.9 VLKK92 pKa = 7.7 TNKK95 pKa = 10.46 SIDD98 pKa = 3.58 DD99 pKa = 3.67 AKK101 pKa = 11.08 KK102 pKa = 10.78 YY103 pKa = 9.13 IAKK106 pKa = 9.67 EE107 pKa = 4.0 DD108 pKa = 3.86 KK109 pKa = 10.91 DD110 pKa = 4.45 NEE112 pKa = 4.26 DD113 pKa = 4.42 LLVSDD118 pKa = 4.32 TSNFIEE124 pKa = 5.0 GVWSKK129 pKa = 9.28 NTIQEE134 pKa = 4.06 ALLDD138 pKa = 4.23 FVKK141 pKa = 10.66 KK142 pKa = 10.08 PSDD145 pKa = 3.38 VAGIIQLYY153 pKa = 8.88 GLKK156 pKa = 10.41 KK157 pKa = 10.56 NPTQIHH163 pKa = 6.1 EE164 pKa = 4.13 EE165 pKa = 4.48 DD166 pKa = 3.66 IPRR169 pKa = 11.84 HH170 pKa = 4.69 AWQIALMKK178 pKa = 9.2 EE179 pKa = 4.5 TEE181 pKa = 4.22 KK182 pKa = 11.07 PPTKK186 pKa = 9.88 KK187 pKa = 9.91 QRR189 pKa = 11.84 RR190 pKa = 11.84 QIIWVVDD197 pKa = 3.67 RR198 pKa = 11.84 VGNTGKK204 pKa = 9.9 SQLAKK209 pKa = 10.63 YY210 pKa = 9.64 MIMSDD215 pKa = 3.33 PKK217 pKa = 10.49 KK218 pKa = 9.99 WFVCKK223 pKa = 10.55 DD224 pKa = 3.18 LGTSRR229 pKa = 11.84 DD230 pKa = 3.44 SSTIIQFAIAKK241 pKa = 8.34 GWEE244 pKa = 4.01 GHH246 pKa = 5.65 GVIIDD251 pKa = 4.07 LPRR254 pKa = 11.84 SAEE257 pKa = 3.89 NHH259 pKa = 5.88 DD260 pKa = 3.55 RR261 pKa = 11.84 MYY263 pKa = 11.2 SYY265 pKa = 10.66 IEE267 pKa = 4.18 EE268 pKa = 4.3 IKK270 pKa = 11.0 DD271 pKa = 3.85 GFVTSQKK278 pKa = 10.42 YY279 pKa = 8.68 QGCTSTFNKK288 pKa = 9.18 PHH290 pKa = 7.16 LIVFANWEE298 pKa = 4.06 PNYY301 pKa = 10.21 TRR303 pKa = 11.84 LSYY306 pKa = 10.83 DD307 pKa = 2.55 RR308 pKa = 11.84 WDD310 pKa = 3.45 MRR312 pKa = 11.84 RR313 pKa = 11.84 LEE315 pKa = 4.42 VKK317 pKa = 10.4 GKK319 pKa = 8.03 TITMEE324 pKa = 4.08 SFIPTILKK332 pKa = 10.09 KK333 pKa = 10.46 KK334 pKa = 8.79 RR335 pKa = 11.84 LEE337 pKa = 3.95 YY338 pKa = 10.43 HH339 pKa = 6.33 AFDD342 pKa = 4.28 YY343 pKa = 10.92 QSQPDD348 pKa = 3.8 EE349 pKa = 4.35 DD350 pKa = 4.34 HH351 pKa = 6.33 LHH353 pKa = 6.89 GYY355 pKa = 8.0 ICKK358 pKa = 10.2 EE359 pKa = 4.24 LMDD362 pKa = 4.77 DD363 pKa = 5.33 LIGEE367 pKa = 4.31 EE368 pKa = 4.64 MLNEE372 pKa = 4.33 HH373 pKa = 7.49 DD374 pKa = 4.76 PGCVDD379 pKa = 4.55 EE380 pKa = 6.1 IEE382 pKa = 4.52 ATDD385 pKa = 4.44 SEE387 pKa = 4.91 VDD389 pKa = 3.76 DD390 pKa = 5.02 TPDD393 pKa = 3.45 DD394 pKa = 4.05 TPEE397 pKa = 3.9 DD398 pKa = 3.97 VYY400 pKa = 11.45 DD401 pKa = 4.2 IDD403 pKa = 5.95 FDD405 pKa = 4.5 DD406 pKa = 5.84 LLVSDD411 pKa = 5.32 DD412 pKa = 4.86 LEE414 pKa = 4.19
Molecular weight: 48.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.241
IPC2_protein 5.194
IPC_protein 5.194
Toseland 5.308
ProMoST 5.359
Dawson 5.283
Bjellqvist 5.385
Wikipedia 5.219
Rodwell 5.219
Grimsley 5.308
Solomon 5.283
Lehninger 5.258
Nozaki 5.448
DTASelect 5.652
Thurlkill 5.372
EMBOSS 5.359
Sillero 5.525
Patrickios 4.19
IPC_peptide 5.296
IPC2_peptide 5.525
IPC2.peptide.svr19 5.472
Protein with the highest isoelectric point:
>tr|A0A346BPB1|A0A346BPB1_9VIRU Putative capsid protein OS=Fire ant associated circular virus 1 OX=2293280 PE=3 SV=1
MM1 pKa = 7.64 SGRR4 pKa = 11.84 KK5 pKa = 7.77 KK6 pKa = 8.83 TASAPAKK13 pKa = 9.93 KK14 pKa = 9.88 YY15 pKa = 9.7 YY16 pKa = 10.11 RR17 pKa = 11.84 YY18 pKa = 9.78 AARR21 pKa = 11.84 RR22 pKa = 11.84 PAVVTGSGDD31 pKa = 3.42 YY32 pKa = 10.29 KK33 pKa = 10.01 VARR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.64 QKK40 pKa = 10.8 SSMRR44 pKa = 11.84 YY45 pKa = 7.76 PGAGKK50 pKa = 8.8 TIGSAIGSVFGPAGSLVGGALGNLAHH76 pKa = 6.71 SALHH80 pKa = 6.92 AITGFGDD87 pKa = 3.2 YY88 pKa = 9.91 HH89 pKa = 6.65 VKK91 pKa = 10.75 HH92 pKa = 6.07 NVLTEE97 pKa = 4.32 TNGPPQVINQGKK109 pKa = 8.97 EE110 pKa = 3.4 FIIRR114 pKa = 11.84 HH115 pKa = 5.5 RR116 pKa = 11.84 EE117 pKa = 3.96 YY118 pKa = 9.71 ITDD121 pKa = 3.86 IYY123 pKa = 11.12 SSAGTASTPSPFLNQIFPINPGQATTFPWLASLAGKK159 pKa = 9.87 FEE161 pKa = 4.23 QYY163 pKa = 10.31 RR164 pKa = 11.84 VDD166 pKa = 3.51 GMVFEE171 pKa = 4.98 FKK173 pKa = 10.5 SLYY176 pKa = 10.09 SDD178 pKa = 4.52 AVVTQNGSIGSIILATEE195 pKa = 4.15 YY196 pKa = 10.86 NAGSAPFNSKK206 pKa = 7.77 QQMEE210 pKa = 4.77 NYY212 pKa = 9.8 QFAQSCKK219 pKa = 8.25 PSLSVLHH226 pKa = 6.76 PIEE229 pKa = 4.4 CARR232 pKa = 11.84 NQTVLSEE239 pKa = 4.39 LYY241 pKa = 9.85 VRR243 pKa = 11.84 PSAVPTGEE251 pKa = 4.7 DD252 pKa = 2.67 IKK254 pKa = 10.24 TYY256 pKa = 11.43 DD257 pKa = 3.44 MGDD260 pKa = 3.52 FQIASQGIPLGSAGAPVNLGEE281 pKa = 4.8 LWVTYY286 pKa = 9.89 QISLLKK292 pKa = 10.36 PKK294 pKa = 10.03 IPSVGPASYY303 pKa = 11.08 VDD305 pKa = 4.0 SGWSHH310 pKa = 7.1 FNTNNLVSGSFTAATPFGPLGSLFPLPQNNILGITFTTNSMSIPLTSTPMRR361 pKa = 11.84 YY362 pKa = 8.99 MIDD365 pKa = 3.46 LWWTGDD371 pKa = 3.57 TNVAGWNAPATGTITNGLLINAQIMTGSYY400 pKa = 10.19 LIPNVGGSATTIGASAKK417 pKa = 10.54 YY418 pKa = 9.95 FIEE421 pKa = 4.78 CPAATGSLNTCLIPIQNNGGFPSATIRR448 pKa = 11.84 RR449 pKa = 11.84 MFSS452 pKa = 2.7
Molecular weight: 48.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.729
IPC2_protein 8.814
IPC_protein 8.726
Toseland 9.107
ProMoST 9.063
Dawson 9.487
Bjellqvist 9.311
Wikipedia 9.706
Rodwell 9.604
Grimsley 9.575
Solomon 9.531
Lehninger 9.472
Nozaki 9.282
DTASelect 9.253
Thurlkill 9.311
EMBOSS 9.575
Sillero 9.458
Patrickios 4.126
IPC_peptide 9.516
IPC2_peptide 8.068
IPC2.peptide.svr19 7.774
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
866
414
452
433.0
48.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.044 ± 1.606
1.039 ± 0.11
6.236 ± 2.716
4.965 ± 1.651
4.388 ± 0.026
7.275 ± 2.073
2.656 ± 0.791
7.275 ± 0.139
6.697 ± 1.94
6.813 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.194 ± 0.013
4.503 ± 0.892
5.658 ± 1.015
3.926 ± 0.04
3.58 ± 0.187
7.852 ± 1.345
7.39 ± 0.41
4.965 ± 0.088
1.617 ± 0.207
3.926 ± 0.198
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here