Primorskyibacter sedentarius

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Primorskyibacter

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4734 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R3IPZ5|A0A4R3IPZ5_9RHOB L-alanine-DL-glutamate epimerase-like enolase superfamily enzyme OS=Primorskyibacter sedentarius OX=745311 GN=EDD52_13820 PE=4 SV=1
MM1 pKa = 7.24PVSVSRR7 pKa = 11.84LSSSSSANPVAVAAVGDD24 pKa = 3.76FDD26 pKa = 6.03GDD28 pKa = 3.67GFEE31 pKa = 5.59DD32 pKa = 5.03FLIGDD37 pKa = 4.72PGNTGDD43 pKa = 3.73GQAYY47 pKa = 10.41LIFGDD52 pKa = 4.29ADD54 pKa = 3.92GLPGDD59 pKa = 4.47IDD61 pKa = 5.69LDD63 pKa = 3.81TLAAGDD69 pKa = 4.28GIKK72 pKa = 10.36VVSPLTDD79 pKa = 3.59DD80 pKa = 3.66QLGMAVSAIGDD91 pKa = 3.55INGDD95 pKa = 3.28GFADD99 pKa = 3.75VMIGAPGTSPEE110 pKa = 3.78YY111 pKa = 10.22QYY113 pKa = 11.07IYY115 pKa = 10.79TRR117 pKa = 11.84FDD119 pKa = 3.33GYY121 pKa = 9.42TRR123 pKa = 11.84FYY125 pKa = 8.18TTYY128 pKa = 10.12IPVTYY133 pKa = 8.88TYY135 pKa = 9.43TYY137 pKa = 9.74TVYY140 pKa = 10.38TSDD143 pKa = 2.88GGTYY147 pKa = 9.38TEE149 pKa = 3.73QRR151 pKa = 11.84TYY153 pKa = 10.11QGGYY157 pKa = 7.72NTYY160 pKa = 9.24QSYY163 pKa = 10.88YY164 pKa = 9.13YY165 pKa = 9.57GTAIQYY171 pKa = 7.86TYY173 pKa = 10.74TRR175 pKa = 11.84TRR177 pKa = 11.84NDD179 pKa = 2.89AGVAYY184 pKa = 10.28VIFGTDD190 pKa = 3.42ADD192 pKa = 4.42PEE194 pKa = 4.58TVDD197 pKa = 3.79VSALGSSGLTVIGPDD212 pKa = 3.37FGGQTGSRR220 pKa = 11.84ISGAADD226 pKa = 3.23FDD228 pKa = 5.01GDD230 pKa = 4.03GKK232 pKa = 10.19PDD234 pKa = 3.41VLINGTATNAAVHH247 pKa = 5.8VLLNPTFEE255 pKa = 4.54ATGSLDD261 pKa = 5.64LYY263 pKa = 10.61DD264 pKa = 3.58QTSGFQRR271 pKa = 11.84LFSTQNDD278 pKa = 3.58SGFGYY283 pKa = 10.74AIDD286 pKa = 4.36AGGDD290 pKa = 3.49INGGFADD297 pKa = 5.06LLIAAPNADD306 pKa = 3.52SGAGVIDD313 pKa = 3.65SGQVFIVFGSDD324 pKa = 2.82APATSRR330 pKa = 11.84QVDD333 pKa = 3.56ALDD336 pKa = 4.31GSDD339 pKa = 3.79GFIFSGQEE347 pKa = 3.6SGDD350 pKa = 3.7LVGSDD355 pKa = 3.37VAFVGDD361 pKa = 4.26SNKK364 pKa = 10.5NGYY367 pKa = 10.4DD368 pKa = 4.01DD369 pKa = 4.35ILIGAAGVGGGRR381 pKa = 11.84GEE383 pKa = 4.28AYY385 pKa = 10.03LVHH388 pKa = 6.83GSPGRR393 pKa = 11.84RR394 pKa = 11.84PADD397 pKa = 4.06FSPADD402 pKa = 3.76LNSAHH407 pKa = 6.93GYY409 pKa = 8.91VISSVGYY416 pKa = 9.97GLGEE420 pKa = 4.04LVGGIGDD427 pKa = 3.86INGDD431 pKa = 3.59GQADD435 pKa = 3.75FALTDD440 pKa = 4.39EE441 pKa = 4.6IATGRR446 pKa = 11.84RR447 pKa = 11.84LHH449 pKa = 6.35IVFGRR454 pKa = 11.84GSGSFNEE461 pKa = 4.26RR462 pKa = 11.84LDD464 pKa = 5.33LEE466 pKa = 4.47TLDD469 pKa = 4.05ARR471 pKa = 11.84TGFTIDD477 pKa = 4.05GSNLTDD483 pKa = 3.84PSSFALSFSGGISAKK498 pKa = 10.13DD499 pKa = 3.26SHH501 pKa = 7.75VSLIIGNGDD510 pKa = 2.82GSTFVISGGYY520 pKa = 7.72ATLAAVDD527 pKa = 4.36RR528 pKa = 11.84LDD530 pKa = 3.78GAQDD534 pKa = 3.55GTIDD538 pKa = 3.39AQYY541 pKa = 8.81FTDD544 pKa = 4.27PFILGSSGNDD554 pKa = 3.34TLNGDD559 pKa = 3.89GSDD562 pKa = 3.16AMVFGFGGDD571 pKa = 3.53DD572 pKa = 3.65SMSGGSGGVALFGGGGNDD590 pKa = 3.19RR591 pKa = 11.84LEE593 pKa = 4.99GGDD596 pKa = 4.4GDD598 pKa = 4.07DD599 pKa = 3.59SAYY602 pKa = 10.62GGSGNDD608 pKa = 3.35MLFGSDD614 pKa = 5.75GEE616 pKa = 4.34DD617 pKa = 3.14QLAGGRR623 pKa = 11.84GNDD626 pKa = 3.61LVHH629 pKa = 6.73GGVGADD635 pKa = 3.56TVSGGAGVDD644 pKa = 3.76TVNGGQGNDD653 pKa = 3.34MLVGGNGDD661 pKa = 4.95DD662 pKa = 3.76ILGGNAGSDD671 pKa = 3.53TIFAGEE677 pKa = 4.11GRR679 pKa = 11.84DD680 pKa = 4.13FIHH683 pKa = 7.03GGFGFDD689 pKa = 3.28QLVGGFGADD698 pKa = 2.72RR699 pKa = 11.84FFHH702 pKa = 6.42LGVRR706 pKa = 11.84GHH708 pKa = 5.59GTDD711 pKa = 3.67WIQDD715 pKa = 3.54YY716 pKa = 10.27DD717 pKa = 3.96ASEE720 pKa = 4.51GDD722 pKa = 3.79VLQSGIAGSSAEE734 pKa = 3.94NYY736 pKa = 9.02VVNFAASSAANAGVVGIADD755 pKa = 3.67AFIGYY760 pKa = 9.55KK761 pKa = 8.82PTGQLLWALTDD772 pKa = 4.21GGDD775 pKa = 3.36QTEE778 pKa = 4.34INIQIDD784 pKa = 3.86GQIFDD789 pKa = 4.81LLSS792 pKa = 3.39

Molecular weight: 80.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R3J832|A0A4R3J832_9RHOB Uncharacterized membrane protein YebE (DUF533 family) OS=Primorskyibacter sedentarius OX=745311 GN=EDD52_11241 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.34SLSAA44 pKa = 3.83

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4734

0

4734

1499026

25

6354

316.7

34.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.891 ± 0.044

0.919 ± 0.01

6.155 ± 0.036

5.883 ± 0.031

3.787 ± 0.019

8.673 ± 0.043

2.054 ± 0.019

5.253 ± 0.025

3.176 ± 0.027

10.049 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.765 ± 0.018

2.713 ± 0.023

5.032 ± 0.03

3.238 ± 0.016

6.585 ± 0.044

5.4 ± 0.025

5.544 ± 0.028

7.217 ± 0.03

1.409 ± 0.015

2.257 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski