Smithella sp. ME-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Syntrophobacterales; Syntrophaceae; Smithella; unclassified Smithella

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 705 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V4F9J5|V4F9J5_9DELT Uncharacterized protein OS=Smithella sp. ME-1 OX=1380771 GN=SMITH_272 PE=4 SV=1
MM1 pKa = 7.43ILSACSNSVLTPAPPQQPSSTSEE24 pKa = 3.76PTKK27 pKa = 10.91EE28 pKa = 4.54EE29 pKa = 4.01IIEE32 pKa = 4.18PNALPTLMATEE43 pKa = 4.42TTVPATPTLEE53 pKa = 3.95PTIVPTEE60 pKa = 3.91VGDD63 pKa = 3.72PNFRR67 pKa = 11.84ILEE70 pKa = 4.27KK71 pKa = 10.76DD72 pKa = 2.91QSEE75 pKa = 4.55QIFIPAGEE83 pKa = 4.82FIMGTQDD90 pKa = 2.51TDD92 pKa = 3.5AQHH95 pKa = 6.33TLDD98 pKa = 4.45NGVAYY103 pKa = 9.63PEE105 pKa = 4.48VPMHH109 pKa = 5.96TVYY112 pKa = 11.2LDD114 pKa = 3.84GYY116 pKa = 9.16WIDD119 pKa = 3.84KK120 pKa = 11.14YY121 pKa = 11.09EE122 pKa = 4.18VTNGRR127 pKa = 11.84YY128 pKa = 8.95ALCVADD134 pKa = 5.09GACTAPWVNFSNTRR148 pKa = 11.84QEE150 pKa = 4.51YY151 pKa = 9.17YY152 pKa = 11.15GNPEE156 pKa = 3.71FDD158 pKa = 3.78NYY160 pKa = 9.38PVIMVDD166 pKa = 2.51WWQSNDD172 pKa = 2.87FCEE175 pKa = 4.39WAGGRR180 pKa = 11.84LPTEE184 pKa = 4.47AEE186 pKa = 3.75WEE188 pKa = 4.16KK189 pKa = 10.84AARR192 pKa = 11.84GTDD195 pKa = 3.1GRR197 pKa = 11.84KK198 pKa = 9.55YY199 pKa = 10.29PWGNDD204 pKa = 2.98PLTEE208 pKa = 4.65DD209 pKa = 3.89KK210 pKa = 11.64ANFCDD215 pKa = 4.25VNCTRR220 pKa = 11.84EE221 pKa = 4.03HH222 pKa = 6.84ANPAFDD228 pKa = 6.01DD229 pKa = 4.56GFADD233 pKa = 3.99TAPVGSYY240 pKa = 10.89PNGASPYY247 pKa = 10.44GVMDD251 pKa = 3.24MAGNVWEE258 pKa = 4.39WTGSIPKK265 pKa = 9.43PYY267 pKa = 9.97PYY269 pKa = 10.74DD270 pKa = 4.5PDD272 pKa = 4.07DD273 pKa = 4.05GRR275 pKa = 11.84EE276 pKa = 3.96EE277 pKa = 4.29NDD279 pKa = 3.36GYY281 pKa = 11.24LYY283 pKa = 10.62VWRR286 pKa = 11.84GGPWSNGTWWIRR298 pKa = 11.84STLRR302 pKa = 11.84YY303 pKa = 9.82KK304 pKa = 10.48SVPKK308 pKa = 10.13YY309 pKa = 9.47WYY311 pKa = 7.99EE312 pKa = 3.85NLGFRR317 pKa = 11.84CASTDD322 pKa = 2.96

Molecular weight: 36.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V4GT14|V4GT14_9DELT Oxygen-insensitive NADPH nitroreductase OS=Smithella sp. ME-1 OX=1380771 GN=SMITH_144 PE=4 SV=1
MM1 pKa = 7.38MNHH4 pKa = 7.03ILNATLLVALIVAFAAGLALGAFYY28 pKa = 10.03FIALWHH34 pKa = 5.5TLQRR38 pKa = 11.84LPAAHH43 pKa = 6.33SPARR47 pKa = 11.84LMLVSFVLRR56 pKa = 11.84MAVVMVGFYY65 pKa = 10.74SIMGGEE71 pKa = 3.59HH72 pKa = 6.13WEE74 pKa = 4.03RR75 pKa = 11.84LVAAMLGFIIIRR87 pKa = 11.84KK88 pKa = 8.98ILTHH92 pKa = 6.76LLGPQKK98 pKa = 9.63MVEE101 pKa = 4.09EE102 pKa = 4.48VRR104 pKa = 11.84CINLGRR110 pKa = 4.05

Molecular weight: 12.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

705

0

705

183521

37

2904

260.3

29.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.59 ± 0.099

1.248 ± 0.042

5.255 ± 0.065

6.379 ± 0.09

4.599 ± 0.075

6.729 ± 0.085

1.825 ± 0.042

8.301 ± 0.099

7.392 ± 0.128

9.439 ± 0.104

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.714 ± 0.043

4.592 ± 0.073

3.997 ± 0.062

3.197 ± 0.052

4.77 ± 0.087

5.982 ± 0.073

4.973 ± 0.062

6.545 ± 0.079

1.101 ± 0.039

3.37 ± 0.059
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski