Amino acid dipepetide frequency for Hymenobacter actinosclerus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	15.294AlaAla: 15.294 ± 0.193	0.781AlaCys: 0.781 ± 0.026	6.139AlaAsp: 6.139 ± 0.079	6.294AlaGlu: 6.294 ± 0.095	3.59AlaPhe: 3.59 ± 0.048	9.622AlaGly: 9.622 ± 0.113	1.986AlaHis: 1.986 ± 0.048	3.816AlaIle: 3.816 ± 0.064	3.317AlaLys: 3.317 ± 0.077	11.872AlaLeu: 11.872 ± 0.12	1.747AlaMet: 1.747 ± 0.045	3.094AlaAsn: 3.094 ± 0.058	5.937AlaPro: 5.937 ± 0.101	4.949AlaGln: 4.949 ± 0.073	7.025AlaArg: 7.025 ± 0.089	5.291AlaSer: 5.291 ± 0.069	6.748AlaThr: 6.748 ± 0.094	7.26AlaVal: 7.26 ± 0.092	1.22AlaTrp: 1.22 ± 0.038	3.168AlaTyr: 3.168 ± 0.059	0.0AlaXaa: 0.0 ± 0.0
Cys	0.642CysAla: 0.642 ± 0.029	0.088CysCys: 0.088 ± 0.008	0.339CysAsp: 0.339 ± 0.017	0.327CysGlu: 0.327 ± 0.017	0.295CysPhe: 0.295 ± 0.015	0.642CysGly: 0.642 ± 0.026	0.189CysHis: 0.189 ± 0.014	0.286CysIle: 0.286 ± 0.016	0.181CysLys: 0.181 ± 0.012	0.713CysLeu: 0.713 ± 0.024	0.108CysMet: 0.108 ± 0.008	0.24CysAsn: 0.24 ± 0.015	0.432CysPro: 0.432 ± 0.021	0.34CysGln: 0.34 ± 0.017	0.451CysArg: 0.451 ± 0.02	0.388CysSer: 0.388 ± 0.015	0.358CysThr: 0.358 ± 0.018	0.43CysVal: 0.43 ± 0.02	0.094CysTrp: 0.094 ± 0.009	0.24CysTyr: 0.24 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	4.98AspAla: 4.98 ± 0.061	0.325AspCys: 0.325 ± 0.015	2.488AspAsp: 2.488 ± 0.053	3.357AspGlu: 3.357 ± 0.058	2.629AspPhe: 2.629 ± 0.053	3.683AspGly: 3.683 ± 0.079	0.803AspHis: 0.803 ± 0.028	2.353AspIle: 2.353 ± 0.048	2.244AspLys: 2.244 ± 0.051	5.065AspLeu: 5.065 ± 0.076	0.969AspMet: 0.969 ± 0.028	1.916AspAsn: 1.916 ± 0.052	2.397AspPro: 2.397 ± 0.045	2.085AspGln: 2.085 ± 0.044	2.472AspArg: 2.472 ± 0.046	2.697AspSer: 2.697 ± 0.053	2.669AspThr: 2.669 ± 0.047	3.547AspVal: 3.547 ± 0.064	0.729AspTrp: 0.729 ± 0.023	2.216AspTyr: 2.216 ± 0.045	0.0AspXaa: 0.0 ± 0.0
Glu	6.049GluAla: 6.049 ± 0.086	0.295GluCys: 0.295 ± 0.016	2.14GluAsp: 2.14 ± 0.049	2.98GluGlu: 2.98 ± 0.063	2.22GluPhe: 2.22 ± 0.046	3.455GluGly: 3.455 ± 0.054	1.183GluHis: 1.183 ± 0.034	2.57GluIle: 2.57 ± 0.055	2.596GluLys: 2.596 ± 0.05	6.891GluLeu: 6.891 ± 0.096	1.3GluMet: 1.3 ± 0.035	1.946GluAsn: 1.946 ± 0.038	2.466GluPro: 2.466 ± 0.053	3.337GluGln: 3.337 ± 0.062	3.317GluArg: 3.317 ± 0.061	2.232GluSer: 2.232 ± 0.047	2.916GluThr: 2.916 ± 0.054	3.89GluVal: 3.89 ± 0.063	0.621GluTrp: 0.621 ± 0.021	1.759GluTyr: 1.759 ± 0.038	0.0GluXaa: 0.0 ± 0.0
Phe	3.654PheAla: 3.654 ± 0.059	0.338PheCys: 0.338 ± 0.016	2.446PheAsp: 2.446 ± 0.049	2.319PheGlu: 2.319 ± 0.045	1.83PhePhe: 1.83 ± 0.042	3.425PheGly: 3.425 ± 0.062	0.7PheHis: 0.7 ± 0.024	1.751PheIle: 1.751 ± 0.041	1.368PheLys: 1.368 ± 0.033	3.85PheLeu: 3.85 ± 0.068	0.768PheMet: 0.768 ± 0.027	1.631PheAsn: 1.631 ± 0.044	1.703PhePro: 1.703 ± 0.042	1.548PheGln: 1.548 ± 0.035	2.699PheArg: 2.699 ± 0.048	2.538PheSer: 2.538 ± 0.053	2.534PheThr: 2.534 ± 0.052	2.895PheVal: 2.895 ± 0.045	0.567PheTrp: 0.567 ± 0.025	1.509PheTyr: 1.509 ± 0.036	0.0PheXaa: 0.0 ± 0.0
Gly	6.831GlyAla: 6.831 ± 0.09	0.614GlyCys: 0.614 ± 0.022	3.017GlyAsp: 3.017 ± 0.076	3.652GlyGlu: 3.652 ± 0.053	3.224GlyPhe: 3.224 ± 0.065	6.179GlyGly: 6.179 ± 0.115	1.596GlyHis: 1.596 ± 0.037	3.6GlyIle: 3.6 ± 0.057	3.268GlyLys: 3.268 ± 0.057	8.953GlyLeu: 8.953 ± 0.113	1.509GlyMet: 1.509 ± 0.039	2.647GlyAsn: 2.647 ± 0.051	3.374GlyPro: 3.374 ± 0.064	4.215GlyGln: 4.215 ± 0.057	5.404GlyArg: 5.404 ± 0.07	4.621GlySer: 4.621 ± 0.082	4.84GlyThr: 4.84 ± 0.087	4.891GlyVal: 4.891 ± 0.066	1.073GlyTrp: 1.073 ± 0.032	2.985GlyTyr: 2.985 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	1.731HisAla: 1.731 ± 0.043	0.205HisCys: 0.205 ± 0.014	1.132HisAsp: 1.132 ± 0.028	1.177HisGlu: 1.177 ± 0.033	0.996HisPhe: 0.996 ± 0.03	1.425HisGly: 1.425 ± 0.037	0.598HisHis: 0.598 ± 0.023	0.892HisIle: 0.892 ± 0.028	0.582HisLys: 0.582 ± 0.021	2.419HisLeu: 2.419 ± 0.048	0.279HisMet: 0.279 ± 0.015	0.673HisAsn: 0.673 ± 0.024	1.273HisPro: 1.273 ± 0.03	0.903HisGln: 0.903 ± 0.028	1.3HisArg: 1.3 ± 0.034	0.977HisSer: 0.977 ± 0.027	1.027HisThr: 1.027 ± 0.031	1.21HisVal: 1.21 ± 0.035	0.274HisTrp: 0.274 ± 0.017	0.865HisTyr: 0.865 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	3.746IleAla: 3.746 ± 0.072	0.388IleCys: 0.388 ± 0.018	2.478IleAsp: 2.478 ± 0.053	2.575IleGlu: 2.575 ± 0.051	1.589IlePhe: 1.589 ± 0.036	3.567IleGly: 3.567 ± 0.064	0.727IleHis: 0.727 ± 0.027	2.312IleIle: 2.312 ± 0.057	1.85IleLys: 1.85 ± 0.046	3.563IleLeu: 3.563 ± 0.057	0.834IleMet: 0.834 ± 0.029	1.741IleAsn: 1.741 ± 0.041	1.981IlePro: 1.981 ± 0.047	1.478IleGln: 1.478 ± 0.039	2.851IleArg: 2.851 ± 0.04	2.801IleSer: 2.801 ± 0.055	2.783IleThr: 2.783 ± 0.05	2.931IleVal: 2.931 ± 0.064	0.449IleTrp: 0.449 ± 0.021	1.293IleTyr: 1.293 ± 0.034	0.0IleXaa: 0.0 ± 0.0
Lys	3.815LysAla: 3.815 ± 0.079	0.181LysCys: 0.181 ± 0.012	1.84LysAsp: 1.84 ± 0.052	2.023LysGlu: 2.023 ± 0.052	1.304LysPhe: 1.304 ± 0.036	2.657LysGly: 2.657 ± 0.059	0.662LysHis: 0.662 ± 0.022	1.8LysIle: 1.8 ± 0.041	2.093LysLys: 2.093 ± 0.062	3.984LysLeu: 3.984 ± 0.058	1.039LysMet: 1.039 ± 0.033	1.493LysAsn: 1.493 ± 0.037	2.001LysPro: 2.001 ± 0.043	1.74LysGln: 1.74 ± 0.047	1.93LysArg: 1.93 ± 0.048	1.997LysSer: 1.997 ± 0.051	2.356LysThr: 2.356 ± 0.043	2.668LysVal: 2.668 ± 0.048	0.367LysTrp: 0.367 ± 0.017	1.298LysTyr: 1.298 ± 0.036	0.0LysXaa: 0.0 ± 0.0
Leu	13.095LeuAla: 13.095 ± 0.136	0.737LeuCys: 0.737 ± 0.031	5.739LeuAsp: 5.739 ± 0.076	5.477LeuGlu: 5.477 ± 0.082	4.056LeuPhe: 4.056 ± 0.064	8.425LeuGly: 8.425 ± 0.101	2.415LeuHis: 2.415 ± 0.046	4.147LeuIle: 4.147 ± 0.067	3.933LeuLys: 3.933 ± 0.07	15.642LeuLeu: 15.642 ± 0.206	2.03LeuMet: 2.03 ± 0.043	4.088LeuAsn: 4.088 ± 0.067	7.137LeuPro: 7.137 ± 0.094	4.291LeuGln: 4.291 ± 0.063	8.468LeuArg: 8.468 ± 0.109	6.541LeuSer: 6.541 ± 0.077	7.268LeuThr: 7.268 ± 0.084	7.673LeuVal: 7.673 ± 0.082	1.091LeuTrp: 1.091 ± 0.033	3.2LeuTyr: 3.2 ± 0.051	0.0LeuXaa: 0.0 ± 0.0
Met	1.914MetAla: 1.914 ± 0.049	0.101MetCys: 0.101 ± 0.01	0.777MetAsp: 0.777 ± 0.026	0.902MetGlu: 0.902 ± 0.029	0.507MetPhe: 0.507 ± 0.021	1.294MetGly: 1.294 ± 0.036	0.395MetHis: 0.395 ± 0.018	0.651MetIle: 0.651 ± 0.028	1.082MetLys: 1.082 ± 0.032	2.182MetLeu: 2.182 ± 0.043	0.428MetMet: 0.428 ± 0.021	0.75MetAsn: 0.75 ± 0.025	1.25MetPro: 1.25 ± 0.033	0.985MetGln: 0.985 ± 0.029	1.301MetArg: 1.301 ± 0.032	1.133MetSer: 1.133 ± 0.028	0.978MetThr: 0.978 ± 0.027	1.201MetVal: 1.201 ± 0.034	0.16MetTrp: 0.16 ± 0.012	0.421MetTyr: 0.421 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.158AsnAla: 3.158 ± 0.054	0.27AsnCys: 0.27 ± 0.014	1.675AsnAsp: 1.675 ± 0.047	1.791AsnGlu: 1.791 ± 0.04	1.512AsnPhe: 1.512 ± 0.038	2.821AsnGly: 2.821 ± 0.06	0.63AsnHis: 0.63 ± 0.023	1.712AsnIle: 1.712 ± 0.043	1.313AsnLys: 1.313 ± 0.037	3.837AsnLeu: 3.837 ± 0.065	0.63AsnMet: 0.63 ± 0.021	1.387AsnAsn: 1.387 ± 0.039	2.415AsnPro: 2.415 ± 0.045	1.643AsnGln: 1.643 ± 0.041	2.228AsnArg: 2.228 ± 0.045	2.036AsnSer: 2.036 ± 0.05	2.055AsnThr: 2.055 ± 0.049	2.39AsnVal: 2.39 ± 0.05	0.477AsnTrp: 0.477 ± 0.023	1.377AsnTyr: 1.377 ± 0.036	0.0AsnXaa: 0.0 ± 0.0
Pro	8.59ProAla: 8.59 ± 0.129	0.25ProCys: 0.25 ± 0.014	3.594ProAsp: 3.594 ± 0.057	3.663ProGlu: 3.663 ± 0.054	1.908ProPhe: 1.908 ± 0.039	4.429ProGly: 4.429 ± 0.068	0.975ProHis: 0.975 ± 0.029	1.935ProIle: 1.935 ± 0.038	1.601ProLys: 1.601 ± 0.036	5.62ProLeu: 5.62 ± 0.09	0.754ProMet: 0.754 ± 0.025	1.878ProAsn: 1.878 ± 0.041	2.484ProPro: 2.484 ± 0.062	1.954ProGln: 1.954 ± 0.04	2.792ProArg: 2.792 ± 0.053	2.234ProSer: 2.234 ± 0.043	3.456ProThr: 3.456 ± 0.059	4.315ProVal: 4.315 ± 0.071	0.574ProTrp: 0.574 ± 0.025	1.572ProTyr: 1.572 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	5.108GlnAla: 5.108 ± 0.08	0.211GlnCys: 0.211 ± 0.013	1.934GlnAsp: 1.934 ± 0.04	2.412GlnGlu: 2.412 ± 0.051	1.784GlnPhe: 1.784 ± 0.036	2.812GlnGly: 2.812 ± 0.048	1.162GlnHis: 1.162 ± 0.033	1.689GlnIle: 1.689 ± 0.045	1.665GlnLys: 1.665 ± 0.041	6.358GlnLeu: 6.358 ± 0.08	0.862GlnMet: 0.862 ± 0.027	1.572GlnAsn: 1.572 ± 0.043	3.15GlnPro: 3.15 ± 0.061	3.583GlnGln: 3.583 ± 0.081	3.254GlnArg: 3.254 ± 0.056	1.878GlnSer: 1.878 ± 0.046	2.452GlnThr: 2.452 ± 0.045	3.349GlnVal: 3.349 ± 0.067	0.523GlnTrp: 0.523 ± 0.026	1.517GlnTyr: 1.517 ± 0.041	0.0GlnXaa: 0.0 ± 0.0
Arg	6.069ArgAla: 6.069 ± 0.075	0.379ArgCys: 0.379 ± 0.016	2.72ArgAsp: 2.72 ± 0.055	3.575ArgGlu: 3.575 ± 0.059	2.814ArgPhe: 2.814 ± 0.05	3.905ArgGly: 3.905 ± 0.067	1.634ArgHis: 1.634 ± 0.042	2.921ArgIle: 2.921 ± 0.049	2.169ArgLys: 2.169 ± 0.057	8.164ArgLeu: 8.164 ± 0.103	1.281ArgMet: 1.281 ± 0.029	2.338ArgAsn: 2.338 ± 0.05	3.905ArgPro: 3.905 ± 0.076	4.111ArgGln: 4.111 ± 0.059	5.095ArgArg: 5.095 ± 0.086	2.647ArgSer: 2.647 ± 0.05	3.615ArgThr: 3.615 ± 0.059	4.434ArgVal: 4.434 ± 0.062	0.905ArgTrp: 0.905 ± 0.032	2.631ArgTyr: 2.631 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	5.402SerAla: 5.402 ± 0.066	0.439SerCys: 0.439 ± 0.022	2.319SerAsp: 2.319 ± 0.044	2.486SerGlu: 2.486 ± 0.043	2.567SerPhe: 2.567 ± 0.052	4.605SerGly: 4.605 ± 0.072	0.957SerHis: 0.957 ± 0.028	2.436SerIle: 2.436 ± 0.047	1.906SerLys: 1.906 ± 0.044	5.696SerLeu: 5.696 ± 0.063	1.012SerMet: 1.012 ± 0.032	1.865SerAsn: 1.865 ± 0.046	2.834SerPro: 2.834 ± 0.05	2.124SerGln: 2.124 ± 0.048	3.404SerArg: 3.404 ± 0.058	3.213SerSer: 3.213 ± 0.063	3.19SerThr: 3.19 ± 0.062	3.749SerVal: 3.749 ± 0.058	0.713SerTrp: 0.713 ± 0.025	2.039SerTyr: 2.039 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	6.701ThrAla: 6.701 ± 0.094	0.3ThrCys: 0.3 ± 0.016	3.415ThrAsp: 3.415 ± 0.05	3.074ThrGlu: 3.074 ± 0.054	2.287ThrPhe: 2.287 ± 0.051	5.109ThrGly: 5.109 ± 0.077	1.057ThrHis: 1.057 ± 0.032	2.647ThrIle: 2.647 ± 0.052	1.858ThrLys: 1.858 ± 0.047	6.626ThrLeu: 6.626 ± 0.077	0.924ThrMet: 0.924 ± 0.027	2.009ThrAsn: 2.009 ± 0.045	3.897ThrPro: 3.897 ± 0.068	2.049ThrGln: 2.049 ± 0.042	3.159ThrArg: 3.159 ± 0.05	3.165ThrSer: 3.165 ± 0.057	3.913ThrThr: 3.913 ± 0.085	4.497ThrVal: 4.497 ± 0.068	0.641ThrTrp: 0.641 ± 0.026	2.063ThrTyr: 2.063 ± 0.045	0.0ThrXaa: 0.0 ± 0.0
Val	8.04ValAla: 8.04 ± 0.103	0.511ValCys: 0.511 ± 0.021	3.062ValAsp: 3.062 ± 0.058	3.693ValGlu: 3.693 ± 0.059	2.793ValPhe: 2.793 ± 0.05	5.204ValGly: 5.204 ± 0.064	1.186ValHis: 1.186 ± 0.034	2.825ValIle: 2.825 ± 0.05	2.577ValLys: 2.577 ± 0.053	8.326ValLeu: 8.326 ± 0.096	1.214ValMet: 1.214 ± 0.036	2.283ValAsn: 2.283 ± 0.047	3.822ValPro: 3.822 ± 0.056	3.125ValGln: 3.125 ± 0.052	4.886ValArg: 4.886 ± 0.069	4.052ValSer: 4.052 ± 0.064	3.762ValThr: 3.762 ± 0.067	5.657ValVal: 5.657 ± 0.086	0.735ValTrp: 0.735 ± 0.024	2.125ValTyr: 2.125 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	1.063TrpAla: 1.063 ± 0.035	0.093TrpCys: 0.093 ± 0.009	0.475TrpAsp: 0.475 ± 0.018	0.561TrpGlu: 0.561 ± 0.024	0.507TrpPhe: 0.507 ± 0.024	0.745TrpGly: 0.745 ± 0.024	0.325TrpHis: 0.325 ± 0.017	0.386TrpIle: 0.386 ± 0.017	0.425TrpLys: 0.425 ± 0.022	1.85TrpLeu: 1.85 ± 0.046	0.236TrpMet: 0.236 ± 0.013	0.439TrpAsn: 0.439 ± 0.02	0.534TrpPro: 0.534 ± 0.026	0.916TrpGln: 0.916 ± 0.027	0.857TrpArg: 0.857 ± 0.031	0.553TrpSer: 0.553 ± 0.019	0.563TrpThr: 0.563 ± 0.022	0.755TrpVal: 0.755 ± 0.03	0.207TrpTrp: 0.207 ± 0.016	0.375TrpTyr: 0.375 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	3.269TyrAla: 3.269 ± 0.056	0.279TyrCys: 0.279 ± 0.014	1.894TyrAsp: 1.894 ± 0.043	1.826TyrGlu: 1.826 ± 0.041	1.659TyrPhe: 1.659 ± 0.033	2.487TyrGly: 2.487 ± 0.042	0.757TyrHis: 0.757 ± 0.024	1.206TyrIle: 1.206 ± 0.029	1.221TyrLys: 1.221 ± 0.036	3.842TyrLeu: 3.842 ± 0.054	0.487TyrMet: 0.487 ± 0.021	1.37TyrAsn: 1.37 ± 0.04	1.585TyrPro: 1.585 ± 0.037	1.903TyrGln: 1.903 ± 0.043	2.421TyrArg: 2.421 ± 0.049	1.962TyrSer: 1.962 ± 0.05	1.905TyrThr: 1.905 ± 0.047	2.136TyrVal: 2.136 ± 0.046	0.444TyrTrp: 0.444 ± 0.021	1.461TyrTyr: 1.461 ± 0.039	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3677 proteins (1251588 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---