Lake Sarah-associated circular virus-31
Average proteome isoelectric point is 8.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140AQN6|A0A140AQN6_9VIRU Coat protein OS=Lake Sarah-associated circular virus-31 OX=1685759 PE=4 SV=1
MM1 pKa = 7.37 SRR3 pKa = 11.84 SQAYY7 pKa = 9.93 CFTVNNYY14 pKa = 9.58 EE15 pKa = 5.02 GLLDD19 pKa = 4.03 WEE21 pKa = 4.77 DD22 pKa = 3.71 LQLAGATYY30 pKa = 10.6 LIYY33 pKa = 10.37 QEE35 pKa = 5.15 EE36 pKa = 4.39 IGEE39 pKa = 4.13 QGTIHH44 pKa = 6.12 LQGYY48 pKa = 9.93 IYY50 pKa = 10.65 FSTKK54 pKa = 10.18 KK55 pKa = 10.65 SMKK58 pKa = 9.47 QLCALIDD65 pKa = 4.04 GSSVRR70 pKa = 11.84 VANGSPQQNTTYY82 pKa = 10.33 CSKK85 pKa = 11.14 EE86 pKa = 3.86 EE87 pKa = 4.0 GRR89 pKa = 11.84 LGGPYY94 pKa = 10.12 VYY96 pKa = 9.89 GTMPQQVRR104 pKa = 11.84 TTSIASAASTARR116 pKa = 11.84 GDD118 pKa = 3.6 KK119 pKa = 10.39 NKK121 pKa = 10.34 KK122 pKa = 6.76 EE123 pKa = 4.24 RR124 pKa = 11.84 KK125 pKa = 9.2 GEE127 pKa = 3.94 EE128 pKa = 3.66 FSRR131 pKa = 11.84 ANSKK135 pKa = 10.74 KK136 pKa = 10.44 NYY138 pKa = 7.98 SAKK141 pKa = 10.34 NFYY144 pKa = 9.87 PPRR147 pKa = 11.84 EE148 pKa = 4.13 KK149 pKa = 10.81 EE150 pKa = 4.32 PTCSPSKK157 pKa = 9.09 PIKK160 pKa = 10.13 MPACPKK166 pKa = 9.64 PNSGTITMPICALSQEE182 pKa = 4.09 RR183 pKa = 11.84 KK184 pKa = 9.47 IYY186 pKa = 10.69 SEE188 pKa = 4.71 LKK190 pKa = 9.56 IKK192 pKa = 9.87 PRR194 pKa = 11.84 NTKK197 pKa = 8.72 PTVLLLVGPSGTGKK211 pKa = 10.35 SRR213 pKa = 11.84 TAYY216 pKa = 10.31 QLAQFLGLTIYY227 pKa = 9.66 VVPPTKK233 pKa = 10.41 GGSGLRR239 pKa = 11.84 FDD241 pKa = 5.75 GYY243 pKa = 10.83 NQQDD247 pKa = 3.4 VCIIDD252 pKa = 4.03 EE253 pKa = 4.28 VDD255 pKa = 3.44 GSSFTPTFFNLLCDD269 pKa = 3.78 WYY271 pKa = 9.3 EE272 pKa = 3.93 MKK274 pKa = 10.99 VPVHH278 pKa = 5.83 GTANLQFTSPYY289 pKa = 10.2 IILCSNYY296 pKa = 9.72 LPKK299 pKa = 10.61 YY300 pKa = 6.42 WWKK303 pKa = 10.59 SRR305 pKa = 11.84 SRR307 pKa = 11.84 DD308 pKa = 3.44 QVKK311 pKa = 8.1 QTTRR315 pKa = 11.84 RR316 pKa = 11.84 IDD318 pKa = 2.91 ITIPFFRR325 pKa = 11.84 RR326 pKa = 11.84 TPQVQPAPSINLPEE340 pKa = 5.21 DD341 pKa = 3.5 LDD343 pKa = 3.75 EE344 pKa = 5.27 FGFVTVHH351 pKa = 6.85 PIKK354 pKa = 10.08 MPHH357 pKa = 6.53 YY358 pKa = 8.1 YY359 pKa = 9.91 VQPLEE364 pKa = 4.2 RR365 pKa = 11.84 FPPKK369 pKa = 9.65 ITDD372 pKa = 3.25 KK373 pKa = 10.86 MIFPLL378 pKa = 4.41
Molecular weight: 42.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.649
IPC2_protein 8.595
IPC_protein 8.463
Toseland 8.931
ProMoST 8.931
Dawson 9.326
Bjellqvist 9.238
Wikipedia 9.502
Rodwell 9.472
Grimsley 9.355
Solomon 9.385
Lehninger 9.326
Nozaki 9.326
DTASelect 9.121
Thurlkill 9.209
EMBOSS 9.443
Sillero 9.385
Patrickios 4.253
IPC_peptide 9.37
IPC2_peptide 8.024
IPC2.peptide.svr19 7.741
Protein with the highest isoelectric point:
>tr|A0A140AQN6|A0A140AQN6_9VIRU Coat protein OS=Lake Sarah-associated circular virus-31 OX=1685759 PE=4 SV=1
MM1 pKa = 7.93 LSRR4 pKa = 11.84 KK5 pKa = 8.61 DD6 pKa = 3.37 LKK8 pKa = 11.17 KK9 pKa = 10.37 IVTKK13 pKa = 10.41 RR14 pKa = 11.84 ASSKK18 pKa = 10.88 ASAASKK24 pKa = 10.23 AARR27 pKa = 11.84 SSNMSARR34 pKa = 11.84 RR35 pKa = 11.84 GPAPTRR41 pKa = 11.84 QAQYY45 pKa = 10.12 ASAVSRR51 pKa = 11.84 SFGSGRR57 pKa = 11.84 EE58 pKa = 3.93 NKK60 pKa = 9.42 TFDD63 pKa = 3.67 AVVLGTNILRR73 pKa = 11.84 ASTDD77 pKa = 3.32 DD78 pKa = 3.46 AVAISASGYY87 pKa = 7.99 ITTNSSAMVLNQVPQSTTSTTRR109 pKa = 11.84 IGRR112 pKa = 11.84 KK113 pKa = 7.7 MVMKK117 pKa = 10.83 GILLQGFIAAGQTGSLGSLARR138 pKa = 11.84 MCLVYY143 pKa = 10.29 IPRR146 pKa = 11.84 LDD148 pKa = 3.51 RR149 pKa = 11.84 TTTTMPPQNVIWSAQNPCALRR170 pKa = 11.84 VINNSDD176 pKa = 3.33 RR177 pKa = 11.84 FRR179 pKa = 11.84 IIRR182 pKa = 11.84 QWTHH186 pKa = 6.1 KK187 pKa = 9.97 LTGDD191 pKa = 3.97 FDD193 pKa = 4.31 AATTGNEE200 pKa = 3.01 IVAFSEE206 pKa = 4.39 YY207 pKa = 11.0 VKK209 pKa = 11.07 LDD211 pKa = 3.72 LQTSWIQSNSDD222 pKa = 2.82 GSFNDD227 pKa = 3.46 MEE229 pKa = 4.58 EE230 pKa = 4.42 GALCLYY236 pKa = 10.64 AQGLYY241 pKa = 9.83 TGASGSAPTINFASRR256 pKa = 11.84 IYY258 pKa = 10.87 FEE260 pKa = 4.96 DD261 pKa = 3.55 HH262 pKa = 6.19
Molecular weight: 28.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.218
IPC2_protein 9.326
IPC_protein 9.472
Toseland 9.999
ProMoST 9.721
Dawson 10.218
Bjellqvist 9.926
Wikipedia 10.394
Rodwell 10.526
Grimsley 10.292
Solomon 10.262
Lehninger 10.218
Nozaki 10.043
DTASelect 9.897
Thurlkill 10.072
EMBOSS 10.409
Sillero 10.145
Patrickios 10.043
IPC_peptide 10.248
IPC2_peptide 8.741
IPC2.peptide.svr19 8.259
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
640
262
378
320.0
35.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.344 ± 2.565
1.875 ± 0.456
4.063 ± 0.323
3.906 ± 1.009
3.906 ± 0.294
6.719 ± 0.095
0.938 ± 0.109
6.094 ± 0.008
6.094 ± 1.184
7.031 ± 0.101
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.5 ± 0.346
4.375 ± 0.366
6.25 ± 1.996
5.156 ± 0.36
5.938 ± 0.821
9.375 ± 1.534
8.125 ± 0.408
4.844 ± 0.074
1.094 ± 0.032
4.375 ± 1.064
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here