Pseudoclavibacter sp. RFBA6

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Pseudoclavibacter; unclassified Pseudoclavibacter

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3544 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S5X8Z1|A0A2S5X8Z1_9MICO Choline dehydrogenase OS=Pseudoclavibacter sp. RFBA6 OX=2080573 GN=C5C17_03740 PE=4 SV=1
MM1 pKa = 7.06SHH3 pKa = 6.61SRR5 pKa = 11.84NQRR8 pKa = 11.84MRR10 pKa = 11.84LSAALALPVAGVLALTGCASGGGDD34 pKa = 3.44TAGGGATEE42 pKa = 4.97FSLTYY47 pKa = 9.26ATSNNLEE54 pKa = 4.09NPYY57 pKa = 10.24EE58 pKa = 4.33ALATAYY64 pKa = 9.78MEE66 pKa = 5.05ANPDD70 pKa = 3.37VKK72 pKa = 9.98ITLNPQPNDD81 pKa = 3.21RR82 pKa = 11.84YY83 pKa = 11.08GEE85 pKa = 4.11TLRR88 pKa = 11.84TQLQAGNAPDD98 pKa = 4.48VIQTAPGSGQGQAVLSLGEE117 pKa = 4.29AGFLAPLGEE126 pKa = 4.35TASGVIPDD134 pKa = 4.18GSEE137 pKa = 3.52ALYY140 pKa = 9.67TYY142 pKa = 10.13EE143 pKa = 4.66DD144 pKa = 3.39QVLAQPVDD152 pKa = 3.87FTVAGFVYY160 pKa = 10.71SNASAEE166 pKa = 4.28AAGVTSYY173 pKa = 10.48PADD176 pKa = 3.54TAEE179 pKa = 5.08LLGKK183 pKa = 10.22CADD186 pKa = 3.84LTSQGKK192 pKa = 9.9SMIVIAGAAGPNTGMTAMSISATRR216 pKa = 11.84VYY218 pKa = 11.23AEE220 pKa = 3.98TPDD223 pKa = 3.5WNEE226 pKa = 3.04QRR228 pKa = 11.84AAGDD232 pKa = 3.32VTFADD237 pKa = 3.68SQGWKK242 pKa = 8.79DD243 pKa = 3.54TLEE246 pKa = 4.4TIIEE250 pKa = 4.31LKK252 pKa = 10.69DD253 pKa = 3.38AGCFQPGAEE262 pKa = 4.04GAGFDD267 pKa = 4.88VITNNITQGTSLGGFLPGSSANEE290 pKa = 3.66LAEE293 pKa = 4.65ANPDD297 pKa = 3.23LDD299 pKa = 4.6FSIEE303 pKa = 4.2PFPSADD309 pKa = 2.97GGKK312 pKa = 9.39PYY314 pKa = 11.2VLASSNYY321 pKa = 9.01SLSINAASEE330 pKa = 4.15KK331 pKa = 10.62QEE333 pKa = 3.88AAQAFLDD340 pKa = 3.95WVAGDD345 pKa = 3.87EE346 pKa = 4.31GAKK349 pKa = 10.42LFSDD353 pKa = 4.06VSGALPVSGIEE364 pKa = 4.0SYY366 pKa = 10.9EE367 pKa = 4.2FEE369 pKa = 4.47GSPYY373 pKa = 11.0ASVEE377 pKa = 4.01QVLTDD382 pKa = 3.59GSYY385 pKa = 10.03TPLPNSLWPNQAVYY399 pKa = 10.99DD400 pKa = 4.09EE401 pKa = 4.55LQKK404 pKa = 11.02GVQGLFTGQTTVDD417 pKa = 3.77SVLSAMDD424 pKa = 4.14AAWDD428 pKa = 3.69SS429 pKa = 3.6

Molecular weight: 44.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S5X5V3|A0A2S5X5V3_9MICO Amino acid ABC transporter permease OS=Pseudoclavibacter sp. RFBA6 OX=2080573 GN=C5C17_08645 PE=3 SV=1
MM1 pKa = 7.51PRR3 pKa = 11.84PRR5 pKa = 11.84RR6 pKa = 11.84GRR8 pKa = 11.84LRR10 pKa = 11.84RR11 pKa = 11.84LRR13 pKa = 11.84VRR15 pKa = 11.84SRR17 pKa = 11.84SRR19 pKa = 11.84RR20 pKa = 11.84STRR23 pKa = 11.84RR24 pKa = 11.84AGLQRR29 pKa = 11.84PRR31 pKa = 11.84RR32 pKa = 11.84LRR34 pKa = 11.84RR35 pKa = 11.84SAPRR39 pKa = 11.84GCRR42 pKa = 11.84RR43 pKa = 11.84PTGARR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84GRR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84CPRR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84EE61 pKa = 3.55PRR63 pKa = 11.84IRR65 pKa = 11.84TRR67 pKa = 11.84WRR69 pKa = 11.84SSRR72 pKa = 11.84LRR74 pKa = 11.84LRR76 pKa = 11.84IRR78 pKa = 11.84LRR80 pKa = 3.54

Molecular weight: 10.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3544

0

3544

1157659

29

3465

326.7

34.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.114 ± 0.051

0.493 ± 0.008

5.855 ± 0.034

6.068 ± 0.034

3.281 ± 0.024

8.927 ± 0.034

1.951 ± 0.017

4.622 ± 0.035

2.11 ± 0.024

10.242 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.817 ± 0.016

2.086 ± 0.021

5.26 ± 0.031

2.992 ± 0.023

6.978 ± 0.043

6.191 ± 0.029

6.094 ± 0.032

8.619 ± 0.033

1.409 ± 0.018

1.891 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski