Neodiprion sertifer nucleopolyhedrovirus
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 90 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q6JKC3|Q6JKC3_9CBAC Uncharacterized protein OS=Neodiprion sertifer nucleopolyhedrovirus OX=111874 PE=4 SV=1
MM1 pKa = 7.65 FSGLRR6 pKa = 11.84 KK7 pKa = 7.27 TAKK10 pKa = 10.12 IYY12 pKa = 11.14 DD13 pKa = 3.77 NTADD17 pKa = 4.31 LLVDD21 pKa = 3.47 NTSLVVGKK29 pKa = 9.29 FSNFDD34 pKa = 3.44 AVFSLPSAKK43 pKa = 10.1 SISKK47 pKa = 10.54 GYY49 pKa = 10.02 VIDD52 pKa = 3.65 YY53 pKa = 7.98 ATFIGNVDD61 pKa = 3.39 TFSINKK67 pKa = 9.21 ILRR70 pKa = 11.84 QADD73 pKa = 3.63 DD74 pKa = 3.79 VNIEE78 pKa = 4.23 TLFNATDD85 pKa = 3.46 TDD87 pKa = 3.77 IAGLNVLRR95 pKa = 11.84 KK96 pKa = 9.31 AANVPDD102 pKa = 3.46 NTIYY106 pKa = 10.07 VAEE109 pKa = 4.45 VKK111 pKa = 10.4 RR112 pKa = 11.84 INLKK116 pKa = 10.27 SLYY119 pKa = 9.63 PSLDD123 pKa = 2.98 VKK125 pKa = 10.37 TYY127 pKa = 11.08 DD128 pKa = 4.96 GIASGLNNNPKK139 pKa = 10.2 LYY141 pKa = 10.57 SYY143 pKa = 11.42 LKK145 pKa = 9.53 GLGVATLAGGAVVLVLLGIDD165 pKa = 4.28 LVQDD169 pKa = 4.39 IIDD172 pKa = 3.82 ALNRR176 pKa = 11.84 TGGSYY181 pKa = 8.39 FTYY184 pKa = 10.97 GEE186 pKa = 4.05 ADD188 pKa = 3.48 NVEE191 pKa = 4.32 SCYY194 pKa = 10.78 LRR196 pKa = 11.84 YY197 pKa = 9.74 RR198 pKa = 11.84 SCGVDD203 pKa = 3.2 QSSVDD208 pKa = 3.39 TTTYY212 pKa = 10.02 CQNFLDD218 pKa = 5.83 PILEE222 pKa = 4.61 DD223 pKa = 4.57 DD224 pKa = 4.1 VTALTAICDD233 pKa = 4.07 GYY235 pKa = 11.29 DD236 pKa = 3.09 IDD238 pKa = 5.06 TEE240 pKa = 4.17 ISVCRR245 pKa = 11.84 QSDD248 pKa = 4.33 PYY250 pKa = 11.27 ADD252 pKa = 4.48 PDD254 pKa = 3.74 SEE256 pKa = 4.18 QWVDD260 pKa = 3.5 VSEE263 pKa = 4.18 LAEE266 pKa = 4.21 NQTLSCVEE274 pKa = 4.62 PYY276 pKa = 10.0 TFSDD280 pKa = 4.78 LISDD284 pKa = 5.54 LGLDD288 pKa = 3.51 WLLGDD293 pKa = 5.76 DD294 pKa = 4.91 SILGNSFTSGSDD306 pKa = 3.6 SISSLSSYY314 pKa = 9.83 IGYY317 pKa = 9.39 IAIFAIIIIVGIVFIKK333 pKa = 10.6 LSKK336 pKa = 10.51 AVSS339 pKa = 3.24
Molecular weight: 36.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.935
IPC_protein 3.973
Toseland 3.732
ProMoST 4.139
Dawson 3.973
Bjellqvist 4.126
Wikipedia 3.935
Rodwell 3.783
Grimsley 3.63
Solomon 3.973
Lehninger 3.923
Nozaki 4.075
DTASelect 4.38
Thurlkill 3.783
EMBOSS 3.935
Sillero 4.088
Patrickios 1.329
IPC_peptide 3.961
IPC2_peptide 4.062
IPC2.peptide.svr19 3.973
Protein with the highest isoelectric point:
>tr|Q6JKE7|Q6JKE7_9CBAC Uncharacterized protein OS=Neodiprion sertifer nucleopolyhedrovirus OX=111874 PE=4 SV=1
MM1 pKa = 7.62 SDD3 pKa = 3.25 SPVFNRR9 pKa = 11.84 SSSRR13 pKa = 11.84 NRR15 pKa = 11.84 RR16 pKa = 11.84 RR17 pKa = 11.84 FNLSLPRR24 pKa = 11.84 AQVVLQRR31 pKa = 11.84 IDD33 pKa = 3.82 DD34 pKa = 4.1 MPDD37 pKa = 2.58 IMALDD42 pKa = 3.85 HH43 pKa = 7.33 QEE45 pKa = 3.84 LNQRR49 pKa = 11.84 KK50 pKa = 8.96 SLIFYY55 pKa = 9.1 LFKK58 pKa = 11.08
Molecular weight: 6.95 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.327
IPC2_protein 9.619
IPC_protein 10.76
Toseland 10.891
ProMoST 11.023
Dawson 10.95
Bjellqvist 10.76
Wikipedia 11.257
Rodwell 10.935
Grimsley 10.994
Solomon 11.228
Lehninger 11.169
Nozaki 10.877
DTASelect 10.76
Thurlkill 10.891
EMBOSS 11.33
Sillero 10.906
Patrickios 10.774
IPC_peptide 11.228
IPC2_peptide 9.897
IPC2.peptide.svr19 8.86
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
90
0
90
24263
54
1143
269.6
31.27
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.446 ± 0.196
2.774 ± 0.182
6.669 ± 0.245
5.185 ± 0.189
4.958 ± 0.193
2.864 ± 0.193
2.506 ± 0.173
9.245 ± 0.244
7.237 ± 0.326
8.68 ± 0.226
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.555 ± 0.135
8.305 ± 0.209
2.745 ± 0.135
3.681 ± 0.138
3.821 ± 0.196
7.089 ± 0.27
6.545 ± 0.28
6.075 ± 0.2
0.717 ± 0.06
4.905 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here