Tubeweb spider associated circular virus 1
Average proteome isoelectric point is 7.51
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A346BP67|A0A346BP67_9VIRU Putative capsid protein OS=Tubeweb spider associated circular virus 1 OX=2293308 PE=4 SV=1
MM1 pKa = 6.51 TRR3 pKa = 11.84 RR4 pKa = 11.84 YY5 pKa = 10.35 KK6 pKa = 10.62 LDD8 pKa = 3.18 NVEE11 pKa = 4.9 YY12 pKa = 10.44 ILLTYY17 pKa = 10.29 SDD19 pKa = 5.02 CPVEE23 pKa = 4.9 FDD25 pKa = 3.46 PQGIIVAVVRR35 pKa = 11.84 SGGVYY40 pKa = 9.4 RR41 pKa = 11.84 LGRR44 pKa = 11.84 EE45 pKa = 3.8 LHH47 pKa = 5.79 QNGMPHH53 pKa = 5.19 YY54 pKa = 9.74 HH55 pKa = 6.46 CFVWWVGGFSHH66 pKa = 7.9 PDD68 pKa = 2.94 AGQLFMVSGRR78 pKa = 11.84 RR79 pKa = 11.84 CNIKK83 pKa = 10.43 KK84 pKa = 8.56 FTANPGRR91 pKa = 11.84 RR92 pKa = 11.84 WDD94 pKa = 3.79 YY95 pKa = 8.01 VGKK98 pKa = 10.48 YY99 pKa = 9.56 AGHH102 pKa = 7.07 KK103 pKa = 9.76 DD104 pKa = 3.16 GHH106 pKa = 5.64 YY107 pKa = 10.66 LIGDD111 pKa = 3.37 QCEE114 pKa = 4.13 RR115 pKa = 11.84 PGGDD119 pKa = 3.31 KK120 pKa = 10.7 DD121 pKa = 4.18 DD122 pKa = 4.47 SEE124 pKa = 4.46 RR125 pKa = 11.84 TQTDD129 pKa = 2.27 IWSEE133 pKa = 3.96 IINATNEE140 pKa = 4.03 GEE142 pKa = 4.33 FFEE145 pKa = 5.14 KK146 pKa = 10.5 LAALAPKK153 pKa = 10.22 QLGCNFGSLKK163 pKa = 10.72 LYY165 pKa = 10.76 ADD167 pKa = 3.52 WKK169 pKa = 9.79 YY170 pKa = 10.4 RR171 pKa = 11.84 PEE173 pKa = 4.03 VADD176 pKa = 3.67 YY177 pKa = 8.97 EE178 pKa = 4.6 SPPGIFEE185 pKa = 4.11 PVYY188 pKa = 11.09 DD189 pKa = 3.77 LTNWSEE195 pKa = 4.07 ANLGRR200 pKa = 11.84 VEE202 pKa = 3.92 GRR204 pKa = 11.84 PRR206 pKa = 11.84 GLVLFGATRR215 pKa = 11.84 LGKK218 pKa = 7.65 TVWARR223 pKa = 11.84 SLGKK227 pKa = 9.74 HH228 pKa = 5.68 NYY230 pKa = 8.93 FAGLFNLDD238 pKa = 4.56 DD239 pKa = 4.25 FSEE242 pKa = 4.36 SAEE245 pKa = 3.92 YY246 pKa = 10.42 AIFDD250 pKa = 4.19 DD251 pKa = 4.0 MSGGFSFFPSYY262 pKa = 9.2 KK263 pKa = 9.6 QWMGGQFQFTVTDD276 pKa = 3.65 KK277 pKa = 10.77 FKK279 pKa = 10.89 HH280 pKa = 5.41 KK281 pKa = 10.03 RR282 pKa = 11.84 TVRR285 pKa = 11.84 WGKK288 pKa = 7.37 PTIWLCNTDD297 pKa = 5.47 PRR299 pKa = 11.84 LDD301 pKa = 3.34 WYY303 pKa = 10.98 KK304 pKa = 10.58 PGSSPDD310 pKa = 3.78 FGWMEE315 pKa = 3.92 EE316 pKa = 4.02 NADD319 pKa = 3.77 FVEE322 pKa = 4.27 LTEE325 pKa = 4.52 TIFRR329 pKa = 11.84 ANRR332 pKa = 11.84 EE333 pKa = 3.96
Molecular weight: 38.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.766
IPC2_protein 5.792
IPC_protein 5.83
Toseland 6.173
ProMoST 6.033
Dawson 6.097
Bjellqvist 6.109
Wikipedia 6.097
Rodwell 6.071
Grimsley 6.313
Solomon 6.097
Lehninger 6.084
Nozaki 6.351
DTASelect 6.547
Thurlkill 6.547
EMBOSS 6.517
Sillero 6.453
Patrickios 4.062
IPC_peptide 6.109
IPC2_peptide 6.427
IPC2.peptide.svr19 6.423
Protein with the highest isoelectric point:
>tr|A0A346BP67|A0A346BP67_9VIRU Putative capsid protein OS=Tubeweb spider associated circular virus 1 OX=2293308 PE=4 SV=1
MM1 pKa = 7.71 AYY3 pKa = 10.24 SRR5 pKa = 11.84 ARR7 pKa = 11.84 TYY9 pKa = 10.45 RR10 pKa = 11.84 RR11 pKa = 11.84 PYY13 pKa = 10.05 RR14 pKa = 11.84 KK15 pKa = 9.38 RR16 pKa = 11.84 SGPKK20 pKa = 9.26 SRR22 pKa = 11.84 RR23 pKa = 11.84 STGKK27 pKa = 9.47 KK28 pKa = 6.78 RR29 pKa = 11.84 TYY31 pKa = 10.71 RR32 pKa = 11.84 KK33 pKa = 7.58 TKK35 pKa = 8.11 KK36 pKa = 8.64 TSRR39 pKa = 11.84 PMSNKK44 pKa = 9.89 KK45 pKa = 9.88 ILDD48 pKa = 3.29 LTSRR52 pKa = 11.84 KK53 pKa = 9.67 KK54 pKa = 10.37 RR55 pKa = 11.84 DD56 pKa = 3.23 TMAPYY61 pKa = 10.24 YY62 pKa = 8.72 NTYY65 pKa = 10.43 GSSPLTGLGGYY76 pKa = 6.44 TANGNGGGAQAYY88 pKa = 7.68 TGLLWLATARR98 pKa = 11.84 DD99 pKa = 3.7 NTLASGGSVSNINDD113 pKa = 3.68 LAARR117 pKa = 11.84 TATACYY123 pKa = 9.63 MRR125 pKa = 11.84 GLKK128 pKa = 10.38 EE129 pKa = 3.84 NVEE132 pKa = 4.36 ISTSSGAPWQWRR144 pKa = 11.84 RR145 pKa = 11.84 ICFTFRR151 pKa = 11.84 DD152 pKa = 4.06 PSVVLSGTGIPPYY165 pKa = 10.77 LEE167 pKa = 4.04 TSNGYY172 pKa = 10.28 ARR174 pKa = 11.84 LLANFTNSDD183 pKa = 3.62 SAGVATHH190 pKa = 6.53 GAITRR195 pKa = 11.84 LIFKK199 pKa = 8.95 GQQNVDD205 pKa = 2.85 WNDD208 pKa = 2.52 IRR210 pKa = 11.84 TAPIDD215 pKa = 3.58 NARR218 pKa = 11.84 VDD220 pKa = 4.01 LKK222 pKa = 10.91 YY223 pKa = 10.74 DD224 pKa = 2.81 KK225 pKa = 11.27 TMIVSSGNANGRR237 pKa = 11.84 VKK239 pKa = 10.41 LFKK242 pKa = 9.42 MWHH245 pKa = 6.16 PMNKK249 pKa = 8.39 TLVYY253 pKa = 10.81 DD254 pKa = 4.05 DD255 pKa = 5.29 DD256 pKa = 4.93 EE257 pKa = 6.01 NGEE260 pKa = 4.4 AEE262 pKa = 4.1 NSTVFSVGDD271 pKa = 3.41 KK272 pKa = 10.75 RR273 pKa = 11.84 GMGDD277 pKa = 3.45 YY278 pKa = 10.86 YY279 pKa = 11.49 VIDD282 pKa = 4.2 FLQAGMGATSSDD294 pKa = 3.51 SLNFNPTATLYY305 pKa = 8.61 WHH307 pKa = 6.59 EE308 pKa = 4.22 RR309 pKa = 3.47
Molecular weight: 34.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.249
IPC2_protein 9.531
IPC_protein 9.736
Toseland 9.984
ProMoST 9.823
Dawson 10.233
Bjellqvist 9.955
Wikipedia 10.438
Rodwell 10.526
Grimsley 10.335
Solomon 10.262
Lehninger 10.218
Nozaki 9.984
DTASelect 9.94
Thurlkill 10.072
EMBOSS 10.409
Sillero 10.145
Patrickios 9.545
IPC_peptide 10.262
IPC2_peptide 8.682
IPC2.peptide.svr19 8.571
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
642
309
333
321.0
36.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.854 ± 1.027
1.246 ± 0.394
6.386 ± 0.369
4.361 ± 1.379
4.829 ± 1.474
9.813 ± 0.282
1.713 ± 0.489
3.583 ± 0.015
5.763 ± 0.254
6.854 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.336 ± 0.379
5.452 ± 0.885
4.517 ± 0.417
2.181 ± 0.37
7.477 ± 0.617
6.854 ± 1.24
7.009 ± 1.563
4.829 ± 0.409
2.804 ± 0.567
5.14 ± 0.238
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here