Streptococcus phage Javan425

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6ABZ1|A0A4D6ABZ1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan425 OX=2548161 GN=Javan425_0049 PE=4 SV=1
MM1 pKa = 7.37WVANDD6 pKa = 3.92YY7 pKa = 11.14EE8 pKa = 4.41DD9 pKa = 5.12LSEE12 pKa = 5.94LFMALDD18 pKa = 3.99TDD20 pKa = 3.45NSMFSDD26 pKa = 4.09PMQSTGLFDD35 pKa = 4.74KK36 pKa = 10.99NALEE40 pKa = 4.09IFEE43 pKa = 4.66GDD45 pKa = 3.52VVNAFDD51 pKa = 4.81YY52 pKa = 11.25DD53 pKa = 3.94SDD55 pKa = 3.91DD56 pKa = 3.47GKK58 pKa = 10.77IYY60 pKa = 9.33KK61 pKa = 8.03TTDD64 pKa = 2.88LTGVITYY71 pKa = 9.1HH72 pKa = 6.82KK73 pKa = 10.57NAFCIQSGKK82 pKa = 10.1ILIDD86 pKa = 3.46LWVHH90 pKa = 6.15AEE92 pKa = 4.3GIEE95 pKa = 4.24IIGNIYY101 pKa = 9.99QNSDD105 pKa = 3.18LLEE108 pKa = 4.46SVEE111 pKa = 4.28EE112 pKa = 4.18

Molecular weight: 12.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AF95|A0A4D6AF95_9CAUD Uncharacterized protein OS=Streptococcus phage Javan425 OX=2548161 GN=Javan425_0037 PE=4 SV=1
MM1 pKa = 7.45TPPTSNLRR9 pKa = 11.84ADD11 pKa = 3.33KK12 pKa = 10.9KK13 pKa = 8.9GTHH16 pKa = 5.72RR17 pKa = 11.84VAFDD21 pKa = 3.43RR22 pKa = 11.84NKK24 pKa = 10.59RR25 pKa = 11.84KK26 pKa = 9.48LLKK29 pKa = 9.25TVNVCGICGKK39 pKa = 9.69PVDD42 pKa = 4.28KK43 pKa = 10.64SLKK46 pKa = 8.99YY47 pKa = 8.81PHH49 pKa = 7.29PLSPAIDD56 pKa = 4.39HH57 pKa = 6.59IVPIAKK63 pKa = 9.92GGHH66 pKa = 6.37PSSMDD71 pKa = 3.44NLQLTHH77 pKa = 6.02WQCNRR82 pKa = 11.84QKK84 pKa = 10.95SDD86 pKa = 3.02KK87 pKa = 10.48LYY89 pKa = 11.19SDD91 pKa = 5.34VKK93 pKa = 10.82QQDD96 pKa = 3.14QKK98 pKa = 10.24TVGNRR103 pKa = 11.84NLPQSRR109 pKa = 11.84DD110 pKa = 2.99WSSYY114 pKa = 9.41ASKK117 pKa = 11.11

Molecular weight: 13.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11437

40

1418

197.2

22.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.357 ± 0.448

0.63 ± 0.114

6.269 ± 0.291

7.1 ± 0.457

4.249 ± 0.244

6.628 ± 0.382

1.381 ± 0.125

7.537 ± 0.316

8.595 ± 0.423

7.913 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.209

5.806 ± 0.21

2.754 ± 0.162

3.672 ± 0.188

3.515 ± 0.238

6.435 ± 0.394

6.829 ± 0.445

6.173 ± 0.203

1.399 ± 0.121

4.223 ± 0.274
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski