Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara) (Halobacterium pharaonis)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Haloarculaceae; Natronomonas; Natronomonas pharaonis

Average proteome isoelectric point is 4.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2764 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U7EY20|A0A1U7EY20_NATPD Uncharacterized protein OS=Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara) OX=348780 GN=NP_3996A PE=4 SV=1
MM1 pKa = 7.51SPDD4 pKa = 3.22QSGSLSRR11 pKa = 11.84RR12 pKa = 11.84TVLVSAVAGGIGAVAGCLDD31 pKa = 4.15GDD33 pKa = 4.05RR34 pKa = 11.84DD35 pKa = 4.14GPDD38 pKa = 3.68PVSLDD43 pKa = 3.99DD44 pKa = 5.08GQACDD49 pKa = 3.5TCDD52 pKa = 3.59MLIEE56 pKa = 4.04MHH58 pKa = 7.07PGPVGQAFYY67 pKa = 11.3GDD69 pKa = 3.94DD70 pKa = 4.11APRR73 pKa = 11.84SLPDD77 pKa = 4.49DD78 pKa = 4.77RR79 pKa = 11.84DD80 pKa = 3.94DD81 pKa = 3.93GAAWFCSSTCAYY93 pKa = 10.44QFILEE98 pKa = 4.15EE99 pKa = 4.33EE100 pKa = 4.43EE101 pKa = 4.64RR102 pKa = 11.84GHH104 pKa = 6.36EE105 pKa = 4.0PAISYY110 pKa = 7.67GTDD113 pKa = 3.13YY114 pKa = 10.99STVDD118 pKa = 3.06YY119 pKa = 10.21TLRR122 pKa = 11.84DD123 pKa = 3.92DD124 pKa = 4.54NGVTVISAHH133 pKa = 6.47LEE135 pKa = 3.67ADD137 pKa = 3.62AFADD141 pKa = 3.68LHH143 pKa = 6.9DD144 pKa = 4.24LTFVADD150 pKa = 3.88SDD152 pKa = 4.76AEE154 pKa = 4.27GAMGGSLIGFSDD166 pKa = 4.03PDD168 pKa = 3.61DD169 pKa = 4.97AEE171 pKa = 4.66AFQSEE176 pKa = 4.61HH177 pKa = 6.92GGEE180 pKa = 4.08LLAHH184 pKa = 6.81DD185 pKa = 4.07EE186 pKa = 4.36VTRR189 pKa = 11.84EE190 pKa = 3.96VLTGLGMGMM199 pKa = 4.51

Molecular weight: 20.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U7EY34|A0A1U7EY34_NATPD DUF2339 family protein OS=Natronomonas pharaonis (strain ATCC 35678 / DSM 2160 / CIP 103997 / JCM 8858 / NBRC 14720 / NCIMB 2260 / Gabara) OX=348780 GN=NP_4038A PE=4 SV=1
MM1 pKa = 7.56NDD3 pKa = 3.67DD4 pKa = 4.09DD5 pKa = 5.68RR6 pKa = 11.84SPGMLSGGLVVPGLLGGLLVVYY28 pKa = 9.34FLLPFVAFLSRR39 pKa = 11.84AGSVDD44 pKa = 3.57VVAHH48 pKa = 6.41LSSPAARR55 pKa = 11.84RR56 pKa = 11.84AVRR59 pKa = 11.84NSLLTAPVATAIATGLGVPLAYY81 pKa = 10.76LLARR85 pKa = 11.84GSFRR89 pKa = 11.84GKK91 pKa = 10.22RR92 pKa = 11.84LLEE95 pKa = 4.16AAVVLPLVAPPVVGGVMLLTAVGQFTPLGRR125 pKa = 11.84VAASLGVPLTDD136 pKa = 3.17SLLGVVLAQLFVAAPFVILTARR158 pKa = 11.84AGFAAIDD165 pKa = 3.75EE166 pKa = 4.49RR167 pKa = 11.84LEE169 pKa = 3.87QASRR173 pKa = 11.84SLGYY177 pKa = 10.88GPIATFWRR185 pKa = 11.84VSLPLASGAIVVGVTLTFVRR205 pKa = 11.84AVGEE209 pKa = 4.57FGATMMVANNPRR221 pKa = 11.84TMPTQIWVDD230 pKa = 4.89FVAGNIDD237 pKa = 4.14GIVPLTLALLAVTLAVVFIVQRR259 pKa = 11.84LGRR262 pKa = 11.84TPAVVDD268 pKa = 3.54AA269 pKa = 5.24

Molecular weight: 27.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2764

0

2764

810134

29

1999

293.1

31.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.416 ± 0.077

0.773 ± 0.016

8.745 ± 0.054

8.847 ± 0.06

3.253 ± 0.032

8.405 ± 0.041

1.97 ± 0.025

4.2 ± 0.033

1.891 ± 0.028

8.779 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.734 ± 0.021

2.211 ± 0.024

4.604 ± 0.035

2.584 ± 0.026

6.37 ± 0.05

5.255 ± 0.035

6.372 ± 0.034

8.893 ± 0.049

1.055 ± 0.017

2.643 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski