Ophiostoma mitovirus 6
Average proteome isoelectric point is 8.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9WJ24|Q9WJ24_9VIRU RNA-dependent RNA polymerase putative OS=Ophiostoma mitovirus 6 OX=88389 GN=pol6 PE=2 SV=1
MM1 pKa = 7.68 KK2 pKa = 10.54 LKK4 pKa = 10.18 QLKK7 pKa = 9.93 LMKK10 pKa = 10.36 NKK12 pKa = 8.95 TYY14 pKa = 10.49 QIIRR18 pKa = 11.84 ILLIVFFPSIKK29 pKa = 9.86 RR30 pKa = 11.84 QTVILNIFMSKK41 pKa = 8.45 INKK44 pKa = 8.04 MIKK47 pKa = 10.4 NNGTLFTVKK56 pKa = 10.22 YY57 pKa = 9.72 LKK59 pKa = 10.15 EE60 pKa = 3.98 LRR62 pKa = 11.84 LHH64 pKa = 4.62 ITKK67 pKa = 10.41 YY68 pKa = 10.55 ISGEE72 pKa = 3.85 PYY74 pKa = 9.8 RR75 pKa = 11.84 NSLNRR80 pKa = 11.84 VSVDD84 pKa = 2.74 KK85 pKa = 11.41 DD86 pKa = 3.56 GFPTLCKK93 pKa = 9.8 EE94 pKa = 4.55 LKK96 pKa = 10.04 VLVNGTYY103 pKa = 9.97 LEE105 pKa = 4.15 KK106 pKa = 10.79 RR107 pKa = 11.84 FVLTIITLSKK117 pKa = 10.85 LLIPQKK123 pKa = 10.56 SEE125 pKa = 4.35 SIPFSTKK132 pKa = 10.34 SITDD136 pKa = 3.08 HH137 pKa = 6.59 WSGIDD142 pKa = 3.45 NISNEE147 pKa = 4.52 EE148 pKa = 3.94 LDD150 pKa = 4.04 KK151 pKa = 11.56 SCSEE155 pKa = 4.33 LNISTRR161 pKa = 11.84 EE162 pKa = 4.04 VQWDD166 pKa = 3.63 VKK168 pKa = 10.78 NFKK171 pKa = 10.51 LLTKK175 pKa = 10.15 AGPHH179 pKa = 6.34 GPQSLTWYY187 pKa = 8.62 HH188 pKa = 6.17 TIKK191 pKa = 10.78 LYY193 pKa = 10.91 DD194 pKa = 3.59 FNQWLGIIGILPKK207 pKa = 10.49 SVLDD211 pKa = 4.46 LFTEE215 pKa = 4.38 TLSYY219 pKa = 10.92 ASKK222 pKa = 10.81 LVLPEE227 pKa = 3.52 IKK229 pKa = 10.65 SNIKK233 pKa = 7.89 STKK236 pKa = 9.5 LIRR239 pKa = 11.84 RR240 pKa = 11.84 LSIVHH245 pKa = 7.03 DD246 pKa = 4.27 PEE248 pKa = 5.07 CKK250 pKa = 9.76 EE251 pKa = 3.73 RR252 pKa = 11.84 VIAIFDD258 pKa = 3.81 YY259 pKa = 10.56 GSQMVLKK266 pKa = 9.85 PIADD270 pKa = 3.65 VLFDD274 pKa = 4.39 LLRR277 pKa = 11.84 NIPSDD282 pKa = 3.38 RR283 pKa = 11.84 TFTQSPFFTHH293 pKa = 6.8 TDD295 pKa = 3.29 LDD297 pKa = 4.03 NKK299 pKa = 10.73 SKK301 pKa = 10.16 FWSIDD306 pKa = 3.18 LSSATDD312 pKa = 3.57 RR313 pKa = 11.84 FPIVFQKK320 pKa = 10.7 RR321 pKa = 11.84 VLQKK325 pKa = 10.61 ILGKK329 pKa = 10.64 QMTDD333 pKa = 2.02 SWEE336 pKa = 4.23 RR337 pKa = 11.84 IMIGSKK343 pKa = 10.25 FLAPDD348 pKa = 4.06 GDD350 pKa = 4.09 TVSYY354 pKa = 11.41 NCGQPMGAQSSWPMFTLAHH373 pKa = 5.52 HH374 pKa = 6.21 VIVRR378 pKa = 11.84 VAANRR383 pKa = 11.84 CGLSNFDD390 pKa = 4.18 KK391 pKa = 11.2 YY392 pKa = 10.94 IILGDD397 pKa = 4.49 DD398 pKa = 3.13 IVINNDD404 pKa = 2.87 NVALKK409 pKa = 10.83 YY410 pKa = 10.03 MEE412 pKa = 4.28 IMNDD416 pKa = 3.37 FKK418 pKa = 11.82 VEE420 pKa = 3.67 ISRR423 pKa = 11.84 NKK425 pKa = 8.42 THH427 pKa = 6.29 VSNDD431 pKa = 2.97 TYY433 pKa = 11.38 EE434 pKa = 4.02 FAKK437 pKa = 10.37 RR438 pKa = 11.84 WIKK441 pKa = 11.14 NKK443 pKa = 9.66 MEE445 pKa = 4.57 FFPLPIRR452 pKa = 11.84 GIVDD456 pKa = 4.22 NINNKK461 pKa = 10.12 YY462 pKa = 10.33 IIFNILYY469 pKa = 10.09 SFFVEE474 pKa = 5.03 KK475 pKa = 11.1 GNTFLNKK482 pKa = 9.01 DD483 pKa = 3.58 TLLVCVSKK491 pKa = 10.4 FIQLHH496 pKa = 5.35 SLTLKK501 pKa = 10.78 KK502 pKa = 10.37 PIGLNKK508 pKa = 9.92 VKK510 pKa = 10.83 GILYY514 pKa = 8.26 PFNFMLRR521 pKa = 11.84 YY522 pKa = 9.13 RR523 pKa = 11.84 QNLCTNEE530 pKa = 4.21 EE531 pKa = 3.52 IRR533 pKa = 11.84 IFLGSSTCKK542 pKa = 10.02 RR543 pKa = 11.84 DD544 pKa = 4.4 DD545 pKa = 3.64 YY546 pKa = 10.8 MLPISAKK553 pKa = 10.24 DD554 pKa = 3.34 VSLEE558 pKa = 3.79 LTRR561 pKa = 11.84 VISAALVGMAYY572 pKa = 10.28 NAEE575 pKa = 4.05 KK576 pKa = 10.48 SLKK579 pKa = 10.37 NIYY582 pKa = 10.11 FDD584 pKa = 5.33 LDD586 pKa = 3.94 KK587 pKa = 11.03 LSPWIGDD594 pKa = 3.49 GFKK597 pKa = 10.03 TGKK600 pKa = 9.13 HH601 pKa = 5.42 PKK603 pKa = 10.33 VMIQSIYY610 pKa = 11.03 NSVKK614 pKa = 10.02 SLSDD618 pKa = 3.63 FGLKK622 pKa = 8.66 MAQNKK627 pKa = 7.56 LTLSAAMDD635 pKa = 3.81 SLLLVDD641 pKa = 5.38 LDD643 pKa = 4.79 SISSSEE649 pKa = 4.16 RR650 pKa = 11.84 IKK652 pKa = 10.59 YY653 pKa = 9.69 IQMKK657 pKa = 10.19 QNICLSQKK665 pKa = 8.61 VRR667 pKa = 11.84 KK668 pKa = 8.13 EE669 pKa = 3.72 LRR671 pKa = 11.84 FDD673 pKa = 4.01 PLQMEE678 pKa = 4.48 QKK680 pKa = 9.9 ARR682 pKa = 11.84 AMMLVKK688 pKa = 10.42 HH689 pKa = 5.94 MKK691 pKa = 10.26 DD692 pKa = 3.29 LEE694 pKa = 4.27 GG695 pKa = 4.62
Molecular weight: 80.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.967
IPC2_protein 8.887
IPC_protein 8.741
Toseland 9.677
ProMoST 9.326
Dawson 9.882
Bjellqvist 9.545
Wikipedia 10.014
Rodwell 10.394
Grimsley 9.94
Solomon 9.897
Lehninger 9.867
Nozaki 9.736
DTASelect 9.516
Thurlkill 9.736
EMBOSS 10.087
Sillero 9.809
Patrickios 9.209
IPC_peptide 9.897
IPC2_peptide 8.039
IPC2.peptide.svr19 7.887
Protein with the highest isoelectric point:
>tr|Q9WJ24|Q9WJ24_9VIRU RNA-dependent RNA polymerase putative OS=Ophiostoma mitovirus 6 OX=88389 GN=pol6 PE=2 SV=1
MM1 pKa = 7.68 KK2 pKa = 10.54 LKK4 pKa = 10.18 QLKK7 pKa = 9.93 LMKK10 pKa = 10.36 NKK12 pKa = 8.95 TYY14 pKa = 10.49 QIIRR18 pKa = 11.84 ILLIVFFPSIKK29 pKa = 9.86 RR30 pKa = 11.84 QTVILNIFMSKK41 pKa = 8.45 INKK44 pKa = 8.04 MIKK47 pKa = 10.4 NNGTLFTVKK56 pKa = 10.22 YY57 pKa = 9.72 LKK59 pKa = 10.15 EE60 pKa = 3.98 LRR62 pKa = 11.84 LHH64 pKa = 4.62 ITKK67 pKa = 10.41 YY68 pKa = 10.55 ISGEE72 pKa = 3.85 PYY74 pKa = 9.8 RR75 pKa = 11.84 NSLNRR80 pKa = 11.84 VSVDD84 pKa = 2.74 KK85 pKa = 11.41 DD86 pKa = 3.56 GFPTLCKK93 pKa = 9.8 EE94 pKa = 4.55 LKK96 pKa = 10.04 VLVNGTYY103 pKa = 9.97 LEE105 pKa = 4.15 KK106 pKa = 10.79 RR107 pKa = 11.84 FVLTIITLSKK117 pKa = 10.85 LLIPQKK123 pKa = 10.56 SEE125 pKa = 4.35 SIPFSTKK132 pKa = 10.34 SITDD136 pKa = 3.08 HH137 pKa = 6.59 WSGIDD142 pKa = 3.45 NISNEE147 pKa = 4.52 EE148 pKa = 3.94 LDD150 pKa = 4.04 KK151 pKa = 11.56 SCSEE155 pKa = 4.33 LNISTRR161 pKa = 11.84 EE162 pKa = 4.04 VQWDD166 pKa = 3.63 VKK168 pKa = 10.78 NFKK171 pKa = 10.51 LLTKK175 pKa = 10.15 AGPHH179 pKa = 6.34 GPQSLTWYY187 pKa = 8.62 HH188 pKa = 6.17 TIKK191 pKa = 10.78 LYY193 pKa = 10.91 DD194 pKa = 3.59 FNQWLGIIGILPKK207 pKa = 10.49 SVLDD211 pKa = 4.46 LFTEE215 pKa = 4.38 TLSYY219 pKa = 10.92 ASKK222 pKa = 10.81 LVLPEE227 pKa = 3.52 IKK229 pKa = 10.65 SNIKK233 pKa = 7.89 STKK236 pKa = 9.5 LIRR239 pKa = 11.84 RR240 pKa = 11.84 LSIVHH245 pKa = 7.03 DD246 pKa = 4.27 PEE248 pKa = 5.07 CKK250 pKa = 9.76 EE251 pKa = 3.73 RR252 pKa = 11.84 VIAIFDD258 pKa = 3.81 YY259 pKa = 10.56 GSQMVLKK266 pKa = 9.85 PIADD270 pKa = 3.65 VLFDD274 pKa = 4.39 LLRR277 pKa = 11.84 NIPSDD282 pKa = 3.38 RR283 pKa = 11.84 TFTQSPFFTHH293 pKa = 6.8 TDD295 pKa = 3.29 LDD297 pKa = 4.03 NKK299 pKa = 10.73 SKK301 pKa = 10.16 FWSIDD306 pKa = 3.18 LSSATDD312 pKa = 3.57 RR313 pKa = 11.84 FPIVFQKK320 pKa = 10.7 RR321 pKa = 11.84 VLQKK325 pKa = 10.61 ILGKK329 pKa = 10.64 QMTDD333 pKa = 2.02 SWEE336 pKa = 4.23 RR337 pKa = 11.84 IMIGSKK343 pKa = 10.25 FLAPDD348 pKa = 4.06 GDD350 pKa = 4.09 TVSYY354 pKa = 11.41 NCGQPMGAQSSWPMFTLAHH373 pKa = 5.52 HH374 pKa = 6.21 VIVRR378 pKa = 11.84 VAANRR383 pKa = 11.84 CGLSNFDD390 pKa = 4.18 KK391 pKa = 11.2 YY392 pKa = 10.94 IILGDD397 pKa = 4.49 DD398 pKa = 3.13 IVINNDD404 pKa = 2.87 NVALKK409 pKa = 10.83 YY410 pKa = 10.03 MEE412 pKa = 4.28 IMNDD416 pKa = 3.37 FKK418 pKa = 11.82 VEE420 pKa = 3.67 ISRR423 pKa = 11.84 NKK425 pKa = 8.42 THH427 pKa = 6.29 VSNDD431 pKa = 2.97 TYY433 pKa = 11.38 EE434 pKa = 4.02 FAKK437 pKa = 10.37 RR438 pKa = 11.84 WIKK441 pKa = 11.14 NKK443 pKa = 9.66 MEE445 pKa = 4.57 FFPLPIRR452 pKa = 11.84 GIVDD456 pKa = 4.22 NINNKK461 pKa = 10.12 YY462 pKa = 10.33 IIFNILYY469 pKa = 10.09 SFFVEE474 pKa = 5.03 KK475 pKa = 11.1 GNTFLNKK482 pKa = 9.01 DD483 pKa = 3.58 TLLVCVSKK491 pKa = 10.4 FIQLHH496 pKa = 5.35 SLTLKK501 pKa = 10.78 KK502 pKa = 10.37 PIGLNKK508 pKa = 9.92 VKK510 pKa = 10.83 GILYY514 pKa = 8.26 PFNFMLRR521 pKa = 11.84 YY522 pKa = 9.13 RR523 pKa = 11.84 QNLCTNEE530 pKa = 4.21 EE531 pKa = 3.52 IRR533 pKa = 11.84 IFLGSSTCKK542 pKa = 10.02 RR543 pKa = 11.84 DD544 pKa = 4.4 DD545 pKa = 3.64 YY546 pKa = 10.8 MLPISAKK553 pKa = 10.24 DD554 pKa = 3.34 VSLEE558 pKa = 3.79 LTRR561 pKa = 11.84 VISAALVGMAYY572 pKa = 10.28 NAEE575 pKa = 4.05 KK576 pKa = 10.48 SLKK579 pKa = 10.37 NIYY582 pKa = 10.11 FDD584 pKa = 5.33 LDD586 pKa = 3.94 KK587 pKa = 11.03 LSPWIGDD594 pKa = 3.49 GFKK597 pKa = 10.03 TGKK600 pKa = 9.13 HH601 pKa = 5.42 PKK603 pKa = 10.33 VMIQSIYY610 pKa = 11.03 NSVKK614 pKa = 10.02 SLSDD618 pKa = 3.63 FGLKK622 pKa = 8.66 MAQNKK627 pKa = 7.56 LTLSAAMDD635 pKa = 3.81 SLLLVDD641 pKa = 5.38 LDD643 pKa = 4.79 SISSSEE649 pKa = 4.16 RR650 pKa = 11.84 IKK652 pKa = 10.59 YY653 pKa = 9.69 IQMKK657 pKa = 10.19 QNICLSQKK665 pKa = 8.61 VRR667 pKa = 11.84 KK668 pKa = 8.13 EE669 pKa = 3.72 LRR671 pKa = 11.84 FDD673 pKa = 4.01 PLQMEE678 pKa = 4.48 QKK680 pKa = 9.9 ARR682 pKa = 11.84 AMMLVKK688 pKa = 10.42 HH689 pKa = 5.94 MKK691 pKa = 10.26 DD692 pKa = 3.29 LEE694 pKa = 4.27 GG695 pKa = 4.62
Molecular weight: 80.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.967
IPC2_protein 8.887
IPC_protein 8.741
Toseland 9.677
ProMoST 9.326
Dawson 9.882
Bjellqvist 9.545
Wikipedia 10.014
Rodwell 10.394
Grimsley 9.94
Solomon 9.897
Lehninger 9.867
Nozaki 9.736
DTASelect 9.516
Thurlkill 9.736
EMBOSS 10.087
Sillero 9.809
Patrickios 9.209
IPC_peptide 9.897
IPC2_peptide 8.039
IPC2.peptide.svr19 7.887
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
695
695
695
695.0
80.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.165 ± 0.0
1.295 ± 0.0
5.755 ± 0.0
3.885 ± 0.0
5.324 ± 0.0
4.029 ± 0.0
1.727 ± 0.0
9.353 ± 0.0
10.072 ± 0.0
11.511 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.309 ± 0.0
5.899 ± 0.0
3.597 ± 0.0
3.309 ± 0.0
4.173 ± 0.0
8.345 ± 0.0
5.18 ± 0.0
5.612 ± 0.0
1.295 ± 0.0
3.165 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here