Wilkie partiti-like virus 2
Average proteome isoelectric point is 7.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z2RTC1|A0A1Z2RTC1_9VIRU RdRp OS=Wilkie partiti-like virus 2 OX=2010284 PE=4 SV=1
MM1 pKa = 7.65 NSYY4 pKa = 10.97 ALNNIIRR11 pKa = 11.84 TSLPNKK17 pKa = 8.85 EE18 pKa = 3.84 RR19 pKa = 11.84 SFRR22 pKa = 11.84 IPDD25 pKa = 3.75 ANHH28 pKa = 6.31 KK29 pKa = 9.66 PEE31 pKa = 4.11 KK32 pKa = 9.91 FALKK36 pKa = 10.23 SIEE39 pKa = 4.32 LIKK42 pKa = 10.52 GSKK45 pKa = 9.5 YY46 pKa = 10.9 ADD48 pKa = 3.69 DD49 pKa = 3.42 VTQNYY54 pKa = 8.64 KK55 pKa = 10.25 RR56 pKa = 11.84 AEE58 pKa = 3.95 ITNEE62 pKa = 3.76 SLISDD67 pKa = 4.61 LYY69 pKa = 10.49 RR70 pKa = 11.84 NNGPGVPHH78 pKa = 6.6 CQDD81 pKa = 2.52 VSYY84 pKa = 11.1 IRR86 pKa = 11.84 ALKK89 pKa = 10.41 LLSSKK94 pKa = 10.87 LLDD97 pKa = 3.72 NGEE100 pKa = 4.21 KK101 pKa = 10.58 LKK103 pKa = 9.87 TVHH106 pKa = 5.7 FTAVRR111 pKa = 11.84 MYY113 pKa = 10.77 DD114 pKa = 3.62 LNKK117 pKa = 10.48 SGSAEE122 pKa = 3.9 LPYY125 pKa = 10.8 VEE127 pKa = 5.07 DD128 pKa = 4.36 AEE130 pKa = 4.71 FINYY134 pKa = 6.1 VHH136 pKa = 6.54 SRR138 pKa = 11.84 FRR140 pKa = 11.84 LGEE143 pKa = 3.97 IEE145 pKa = 4.52 NLSLSKK151 pKa = 11.23 GNGWNMILEE160 pKa = 4.15 KK161 pKa = 10.64 EE162 pKa = 4.25 RR163 pKa = 11.84 IKK165 pKa = 10.43 IHH167 pKa = 5.62 QIKK170 pKa = 10.32 DD171 pKa = 2.97 KK172 pKa = 10.23 RR173 pKa = 11.84 LPFIKK178 pKa = 9.57 MVHH181 pKa = 5.66 DD182 pKa = 3.75 TRR184 pKa = 11.84 MHH186 pKa = 5.92 ARR188 pKa = 11.84 SHH190 pKa = 5.64 LTTVEE195 pKa = 3.98 KK196 pKa = 10.52 ADD198 pKa = 3.59 KK199 pKa = 10.24 VRR201 pKa = 11.84 AVYY204 pKa = 9.05 GVCCTIILAEE214 pKa = 5.36 IMILWPLINRR224 pKa = 11.84 IKK226 pKa = 11.08 SMNDD230 pKa = 2.88 PFIAWGFEE238 pKa = 3.99 TFRR241 pKa = 11.84 GGLQKK246 pKa = 10.04 IRR248 pKa = 11.84 EE249 pKa = 4.36 RR250 pKa = 11.84 VCEE253 pKa = 3.9 YY254 pKa = 10.76 DD255 pKa = 3.16 VHH257 pKa = 7.84 LNLDD261 pKa = 4.17 FSTFDD266 pKa = 3.26 KK267 pKa = 11.23 LIPFWLIDD275 pKa = 5.54 DD276 pKa = 4.26 IHH278 pKa = 6.42 QVWKK282 pKa = 10.13 EE283 pKa = 3.75 NYY285 pKa = 9.5 EE286 pKa = 3.73 IGPYY290 pKa = 10.15 YY291 pKa = 10.29 EE292 pKa = 5.66 DD293 pKa = 3.83 DD294 pKa = 3.5 PRR296 pKa = 11.84 YY297 pKa = 9.02 PRR299 pKa = 11.84 PKK301 pKa = 8.19 TTPEE305 pKa = 4.14 RR306 pKa = 11.84 INNIWEE312 pKa = 4.17 FVNFSTKK319 pKa = 8.36 HH320 pKa = 4.3 TPYY323 pKa = 10.3 RR324 pKa = 11.84 APDD327 pKa = 3.43 GSRR330 pKa = 11.84 YY331 pKa = 9.12 LRR333 pKa = 11.84 KK334 pKa = 9.82 HH335 pKa = 5.73 SGIASGQLQTQLLGSCVNYY354 pKa = 10.24 VIIVSALLDD363 pKa = 3.95 FGLSEE368 pKa = 4.72 DD369 pKa = 3.57 QFKK372 pKa = 11.04 VYY374 pKa = 11.11 VMGDD378 pKa = 3.35 DD379 pKa = 3.63 SLISIRR385 pKa = 11.84 YY386 pKa = 8.75 VNDD389 pKa = 3.33 PKK391 pKa = 10.71 IILSHH396 pKa = 6.55 ISVYY400 pKa = 10.65 VKK402 pKa = 10.68 HH403 pKa = 6.27 NFNAIVNPDD412 pKa = 3.24 KK413 pKa = 11.14 CSISVGPDD421 pKa = 3.14 SIQFLGYY428 pKa = 9.84 KK429 pKa = 10.06 FKK431 pKa = 11.12 DD432 pKa = 3.65 GAITRR437 pKa = 11.84 VNNDD441 pKa = 3.2 LLAKK445 pKa = 10.32 LVYY448 pKa = 9.7 PEE450 pKa = 4.54 KK451 pKa = 10.97 AHH453 pKa = 6.26 FTLEE457 pKa = 4.33 STKK460 pKa = 10.72 SRR462 pKa = 11.84 AAGILLANLGYY473 pKa = 10.76 DD474 pKa = 3.72 LDD476 pKa = 3.85 IHH478 pKa = 6.71 VICCNILRR486 pKa = 11.84 KK487 pKa = 10.16 LEE489 pKa = 3.98 STAINLKK496 pKa = 9.49 GLNWHH501 pKa = 5.58 DD502 pKa = 3.73 RR503 pKa = 11.84 KK504 pKa = 10.61 RR505 pKa = 11.84 IEE507 pKa = 3.99 YY508 pKa = 9.62 YY509 pKa = 9.72 ISNFIDD515 pKa = 3.41 TPTRR519 pKa = 11.84 DD520 pKa = 3.61 DD521 pKa = 4.07 LFRR524 pKa = 11.84 TALTPQDD531 pKa = 3.82 YY532 pKa = 10.45 PGDD535 pKa = 3.87 QNWRR539 pKa = 11.84 KK540 pKa = 9.0 FLRR543 pKa = 4.09
Molecular weight: 62.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.979
IPC2_protein 8.112
IPC_protein 7.98
Toseland 8.229
ProMoST 8.595
Dawson 8.814
Bjellqvist 8.873
Wikipedia 8.873
Rodwell 8.902
Grimsley 8.39
Solomon 8.887
Lehninger 8.873
Nozaki 8.96
DTASelect 8.712
Thurlkill 8.799
EMBOSS 8.975
Sillero 9.019
Patrickios 4.418
IPC_peptide 8.887
IPC2_peptide 7.614
IPC2.peptide.svr19 7.709
Protein with the highest isoelectric point:
>tr|A0A1Z2RTC1|A0A1Z2RTC1_9VIRU RdRp OS=Wilkie partiti-like virus 2 OX=2010284 PE=4 SV=1
MM1 pKa = 7.65 NSYY4 pKa = 10.97 ALNNIIRR11 pKa = 11.84 TSLPNKK17 pKa = 8.85 EE18 pKa = 3.84 RR19 pKa = 11.84 SFRR22 pKa = 11.84 IPDD25 pKa = 3.75 ANHH28 pKa = 6.31 KK29 pKa = 9.66 PEE31 pKa = 4.11 KK32 pKa = 9.91 FALKK36 pKa = 10.23 SIEE39 pKa = 4.32 LIKK42 pKa = 10.52 GSKK45 pKa = 9.5 YY46 pKa = 10.9 ADD48 pKa = 3.69 DD49 pKa = 3.42 VTQNYY54 pKa = 8.64 KK55 pKa = 10.25 RR56 pKa = 11.84 AEE58 pKa = 3.95 ITNEE62 pKa = 3.76 SLISDD67 pKa = 4.61 LYY69 pKa = 10.49 RR70 pKa = 11.84 NNGPGVPHH78 pKa = 6.6 CQDD81 pKa = 2.52 VSYY84 pKa = 11.1 IRR86 pKa = 11.84 ALKK89 pKa = 10.41 LLSSKK94 pKa = 10.87 LLDD97 pKa = 3.72 NGEE100 pKa = 4.21 KK101 pKa = 10.58 LKK103 pKa = 9.87 TVHH106 pKa = 5.7 FTAVRR111 pKa = 11.84 MYY113 pKa = 10.77 DD114 pKa = 3.62 LNKK117 pKa = 10.48 SGSAEE122 pKa = 3.9 LPYY125 pKa = 10.8 VEE127 pKa = 5.07 DD128 pKa = 4.36 AEE130 pKa = 4.71 FINYY134 pKa = 6.1 VHH136 pKa = 6.54 SRR138 pKa = 11.84 FRR140 pKa = 11.84 LGEE143 pKa = 3.97 IEE145 pKa = 4.52 NLSLSKK151 pKa = 11.23 GNGWNMILEE160 pKa = 4.15 KK161 pKa = 10.64 EE162 pKa = 4.25 RR163 pKa = 11.84 IKK165 pKa = 10.43 IHH167 pKa = 5.62 QIKK170 pKa = 10.32 DD171 pKa = 2.97 KK172 pKa = 10.23 RR173 pKa = 11.84 LPFIKK178 pKa = 9.57 MVHH181 pKa = 5.66 DD182 pKa = 3.75 TRR184 pKa = 11.84 MHH186 pKa = 5.92 ARR188 pKa = 11.84 SHH190 pKa = 5.64 LTTVEE195 pKa = 3.98 KK196 pKa = 10.52 ADD198 pKa = 3.59 KK199 pKa = 10.24 VRR201 pKa = 11.84 AVYY204 pKa = 9.05 GVCCTIILAEE214 pKa = 5.36 IMILWPLINRR224 pKa = 11.84 IKK226 pKa = 11.08 SMNDD230 pKa = 2.88 PFIAWGFEE238 pKa = 3.99 TFRR241 pKa = 11.84 GGLQKK246 pKa = 10.04 IRR248 pKa = 11.84 EE249 pKa = 4.36 RR250 pKa = 11.84 VCEE253 pKa = 3.9 YY254 pKa = 10.76 DD255 pKa = 3.16 VHH257 pKa = 7.84 LNLDD261 pKa = 4.17 FSTFDD266 pKa = 3.26 KK267 pKa = 11.23 LIPFWLIDD275 pKa = 5.54 DD276 pKa = 4.26 IHH278 pKa = 6.42 QVWKK282 pKa = 10.13 EE283 pKa = 3.75 NYY285 pKa = 9.5 EE286 pKa = 3.73 IGPYY290 pKa = 10.15 YY291 pKa = 10.29 EE292 pKa = 5.66 DD293 pKa = 3.83 DD294 pKa = 3.5 PRR296 pKa = 11.84 YY297 pKa = 9.02 PRR299 pKa = 11.84 PKK301 pKa = 8.19 TTPEE305 pKa = 4.14 RR306 pKa = 11.84 INNIWEE312 pKa = 4.17 FVNFSTKK319 pKa = 8.36 HH320 pKa = 4.3 TPYY323 pKa = 10.3 RR324 pKa = 11.84 APDD327 pKa = 3.43 GSRR330 pKa = 11.84 YY331 pKa = 9.12 LRR333 pKa = 11.84 KK334 pKa = 9.82 HH335 pKa = 5.73 SGIASGQLQTQLLGSCVNYY354 pKa = 10.24 VIIVSALLDD363 pKa = 3.95 FGLSEE368 pKa = 4.72 DD369 pKa = 3.57 QFKK372 pKa = 11.04 VYY374 pKa = 11.11 VMGDD378 pKa = 3.35 DD379 pKa = 3.63 SLISIRR385 pKa = 11.84 YY386 pKa = 8.75 VNDD389 pKa = 3.33 PKK391 pKa = 10.71 IILSHH396 pKa = 6.55 ISVYY400 pKa = 10.65 VKK402 pKa = 10.68 HH403 pKa = 6.27 NFNAIVNPDD412 pKa = 3.24 KK413 pKa = 11.14 CSISVGPDD421 pKa = 3.14 SIQFLGYY428 pKa = 9.84 KK429 pKa = 10.06 FKK431 pKa = 11.12 DD432 pKa = 3.65 GAITRR437 pKa = 11.84 VNNDD441 pKa = 3.2 LLAKK445 pKa = 10.32 LVYY448 pKa = 9.7 PEE450 pKa = 4.54 KK451 pKa = 10.97 AHH453 pKa = 6.26 FTLEE457 pKa = 4.33 STKK460 pKa = 10.72 SRR462 pKa = 11.84 AAGILLANLGYY473 pKa = 10.76 DD474 pKa = 3.72 LDD476 pKa = 3.85 IHH478 pKa = 6.71 VICCNILRR486 pKa = 11.84 KK487 pKa = 10.16 LEE489 pKa = 3.98 STAINLKK496 pKa = 9.49 GLNWHH501 pKa = 5.58 DD502 pKa = 3.73 RR503 pKa = 11.84 KK504 pKa = 10.61 RR505 pKa = 11.84 IEE507 pKa = 3.99 YY508 pKa = 9.62 YY509 pKa = 9.72 ISNFIDD515 pKa = 3.41 TPTRR519 pKa = 11.84 DD520 pKa = 3.61 DD521 pKa = 4.07 LFRR524 pKa = 11.84 TALTPQDD531 pKa = 3.82 YY532 pKa = 10.45 PGDD535 pKa = 3.87 QNWRR539 pKa = 11.84 KK540 pKa = 9.0 FLRR543 pKa = 4.09
Molecular weight: 62.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.979
IPC2_protein 8.112
IPC_protein 7.98
Toseland 8.229
ProMoST 8.595
Dawson 8.814
Bjellqvist 8.873
Wikipedia 8.873
Rodwell 8.902
Grimsley 8.39
Solomon 8.887
Lehninger 8.873
Nozaki 8.96
DTASelect 8.712
Thurlkill 8.799
EMBOSS 8.975
Sillero 9.019
Patrickios 4.418
IPC_peptide 8.887
IPC2_peptide 7.614
IPC2.peptide.svr19 7.709
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
543
543
543
543.0
62.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.788 ± 0.0
1.473 ± 0.0
6.814 ± 0.0
5.157 ± 0.0
4.236 ± 0.0
4.788 ± 0.0
3.131 ± 0.0
9.024 ± 0.0
7.366 ± 0.0
9.761 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.473 ± 0.0
6.446 ± 0.0
4.42 ± 0.0
2.21 ± 0.0
6.262 ± 0.0
6.63 ± 0.0
4.42 ± 0.0
5.341 ± 0.0
1.473 ± 0.0
4.788 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here