Chondrocystis sp. NIES-4102
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4342 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Z4RSU1|A0A1Z4RSU1_9CHRO Putative ABC transporter permease protein OS=Chondrocystis sp. NIES-4102 OX=2005460 GN=NIES4102_25340 PE=3 SV=1
MM1 pKa = 7.58 EE2 pKa = 5.89 LFSNNNLIRR11 pKa = 11.84 THH13 pKa = 7.07 FLLQSEE19 pKa = 4.81 WEE21 pKa = 3.94 QGFTGKK27 pKa = 10.78 LEE29 pKa = 4.04 LTNTGEE35 pKa = 4.37 TLKK38 pKa = 10.75 DD39 pKa = 3.16 WQIEE43 pKa = 4.4 FEE45 pKa = 4.51 SSWEE49 pKa = 3.88 ITPGMIWGAEE59 pKa = 4.05 IVSHH63 pKa = 6.13 QGNKK67 pKa = 9.75 YY68 pKa = 7.91 VLKK71 pKa = 10.28 PVDD74 pKa = 4.06 YY75 pKa = 10.71 NATIYY80 pKa = 10.38 SQQQIAIIFNANKK93 pKa = 9.71 IDD95 pKa = 3.81 GQVGNPTNIIFSDD108 pKa = 4.32 DD109 pKa = 3.74 YY110 pKa = 11.74 GLPTPPSNSSDD121 pKa = 3.2 NSNSEE126 pKa = 4.45 VINTNDD132 pKa = 4.99 DD133 pKa = 3.6 LATDD137 pKa = 3.5 IDD139 pKa = 4.03 FTLVKK144 pKa = 10.31 DD145 pKa = 3.39 WGSGFEE151 pKa = 4.15 GQISITNNSPYY162 pKa = 10.75 SIDD165 pKa = 3.15 SWTLAFDD172 pKa = 4.43 FPNPINNIWDD182 pKa = 3.73 AEE184 pKa = 4.31 IEE186 pKa = 4.28 SSTNGSYY193 pKa = 9.92 TIKK196 pKa = 10.04 NAAWNRR202 pKa = 11.84 EE203 pKa = 3.78 LSAGEE208 pKa = 4.15 TITFGFTGYY217 pKa = 11.01 NSVTSQPQNFDD228 pKa = 3.51 LDD230 pKa = 3.75 GSTFASPSTSDD241 pKa = 2.93 SKK243 pKa = 11.59 YY244 pKa = 10.63 SYY246 pKa = 11.16 SNPDD250 pKa = 2.92 LTTEE254 pKa = 4.46 LEE256 pKa = 4.25 LNKK259 pKa = 10.21 NYY261 pKa = 10.1 QGRR264 pKa = 11.84 ATFYY268 pKa = 11.01 DD269 pKa = 3.46 AANPSGGIGASGYY282 pKa = 10.18 DD283 pKa = 3.86 VPSLDD288 pKa = 3.38 QLYY291 pKa = 10.4 KK292 pKa = 10.68 VVAINNIQWNGSEE305 pKa = 3.8 ASGGFFEE312 pKa = 5.63 VSGPKK317 pKa = 9.39 QRR319 pKa = 11.84 EE320 pKa = 3.63 GAAPVIVQVIDD331 pKa = 3.56 YY332 pKa = 9.92 LYY334 pKa = 10.72 EE335 pKa = 4.31 RR336 pKa = 11.84 ADD338 pKa = 3.66 GMDD341 pKa = 3.34 MSAEE345 pKa = 3.96 AFKK348 pKa = 11.09 LVADD352 pKa = 4.18 PVDD355 pKa = 4.37 GIVNINYY362 pKa = 9.66 QLVGPDD368 pKa = 3.73 DD369 pKa = 5.56 DD370 pKa = 5.02 YY371 pKa = 11.64 VTAYY375 pKa = 10.07 GYY377 pKa = 10.81 SIGQGIVVEE386 pKa = 6.01 GIAEE390 pKa = 4.26 SNPYY394 pKa = 8.53 YY395 pKa = 10.85 AAVRR399 pKa = 11.84 LNNYY403 pKa = 8.62 RR404 pKa = 11.84 YY405 pKa = 9.49 PIEE408 pKa = 4.14 TVEE411 pKa = 4.48 LLTEE415 pKa = 4.09 NGEE418 pKa = 4.51 AIDD421 pKa = 5.26 LNRR424 pKa = 11.84 EE425 pKa = 3.62 SDD427 pKa = 3.45 NRR429 pKa = 11.84 FVLDD433 pKa = 3.9 GNYY436 pKa = 9.92 PLYY439 pKa = 10.6 GAQDD443 pKa = 4.12 LLVTDD448 pKa = 4.2 IFGQQVTLDD457 pKa = 4.22 DD458 pKa = 4.08 VNITNGSSNDD468 pKa = 3.68 TVTGEE473 pKa = 4.04 QFAKK477 pKa = 10.41 IAA479 pKa = 3.88
Molecular weight: 52.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.706
IPC2_protein 3.821
IPC_protein 3.834
Toseland 3.617
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.732
Rodwell 3.656
Grimsley 3.528
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.151
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.948
Patrickios 1.087
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.839
Protein with the highest isoelectric point:
>tr|A0A1Z4RKU0|A0A1Z4RKU0_9CHRO Uncharacterized protein OS=Chondrocystis sp. NIES-4102 OX=2005460 GN=NIES4102_00810 PE=4 SV=1
MM1 pKa = 6.61 TQRR4 pKa = 11.84 TLGGTNRR11 pKa = 11.84 KK12 pKa = 7.61 QKK14 pKa = 8.99 RR15 pKa = 11.84 TSGFRR20 pKa = 11.84 ARR22 pKa = 11.84 MRR24 pKa = 11.84 TSNGKK29 pKa = 9.45 RR30 pKa = 11.84 VIQARR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 9.07 KK38 pKa = 9.65 GRR40 pKa = 11.84 HH41 pKa = 5.0 RR42 pKa = 11.84 LSVV45 pKa = 3.12
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4342
0
4342
1365604
29
10077
314.5
35.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.685 ± 0.039
0.984 ± 0.018
5.074 ± 0.043
6.071 ± 0.039
3.82 ± 0.023
6.308 ± 0.048
1.698 ± 0.021
7.905 ± 0.034
5.444 ± 0.04
10.963 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.763 ± 0.021
5.124 ± 0.047
4.24 ± 0.03
5.565 ± 0.039
4.428 ± 0.034
6.463 ± 0.031
5.711 ± 0.042
6.078 ± 0.03
1.357 ± 0.017
3.321 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here