Streptococcus satellite phage Javan43
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 24 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZML7|A0A4D5ZML7_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan43 OX=2558698 GN=JavanS43_0015 PE=4 SV=1
MM1 pKa = 7.55 RR2 pKa = 11.84 TFSDD6 pKa = 3.67 TPKK9 pKa = 10.07 TFTFRR14 pKa = 11.84 YY15 pKa = 7.02 TFRR18 pKa = 11.84 DD19 pKa = 3.21 FDD21 pKa = 4.07 TAQVACHH28 pKa = 6.88 AILGYY33 pKa = 7.77 MTGTYY38 pKa = 7.11 EE39 pKa = 4.76 QPVIDD44 pKa = 3.97 ATYY47 pKa = 10.82 HH48 pKa = 6.24 NDD50 pKa = 3.59 DD51 pKa = 3.72 QGGHH55 pKa = 6.63 ANQLVLKK62 pKa = 9.52 YY63 pKa = 10.97 AEE65 pKa = 4.31 DD66 pKa = 3.69 RR67 pKa = 11.84 KK68 pKa = 10.48 LSKK71 pKa = 10.32 VFKK74 pKa = 10.15 RR75 pKa = 11.84 ICDD78 pKa = 3.69 SFKK81 pKa = 11.02 DD82 pKa = 4.37 YY83 pKa = 11.5 YY84 pKa = 10.49 NQPADD89 pKa = 3.67 MTDD92 pKa = 3.31 EE93 pKa = 4.71 EE94 pKa = 5.66 LDD96 pKa = 5.27 DD97 pKa = 3.9 MDD99 pKa = 5.64 QEE101 pKa = 4.27 NEE103 pKa = 4.22 LIKK106 pKa = 10.18 EE107 pKa = 4.19 VKK109 pKa = 8.91 MCNDD113 pKa = 2.83 NQLFTKK119 pKa = 10.13 EE120 pKa = 4.06 YY121 pKa = 7.62 IAKK124 pKa = 8.7 RR125 pKa = 11.84 TTQLEE130 pKa = 4.71 DD131 pKa = 3.03 IDD133 pKa = 4.01 KK134 pKa = 10.12 TILIRR139 pKa = 11.84 DD140 pKa = 4.19 IIAYY144 pKa = 9.18 EE145 pKa = 4.47 LEE147 pKa = 4.08 LLDD150 pKa = 3.89 YY151 pKa = 11.08 ADD153 pKa = 5.25 RR154 pKa = 11.84 LLSDD158 pKa = 3.43 KK159 pKa = 10.57 SIRR162 pKa = 11.84 MDD164 pKa = 3.36 SEE166 pKa = 4.29 TAQGTIEE173 pKa = 5.02 LMDD176 pKa = 5.05 DD177 pKa = 3.42 NVINLVKK184 pKa = 10.53 EE185 pKa = 3.94 LDD187 pKa = 3.67 TEE189 pKa = 4.21 RR190 pKa = 11.84 EE191 pKa = 4.1 YY192 pKa = 11.12 QGLHH196 pKa = 6.21 NYY198 pKa = 9.05 VISS201 pKa = 3.68
Molecular weight: 23.57 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.416
IPC2_protein 4.418
IPC_protein 4.38
Toseland 4.19
ProMoST 4.52
Dawson 4.355
Bjellqvist 4.507
Wikipedia 4.266
Rodwell 4.215
Grimsley 4.101
Solomon 4.355
Lehninger 4.304
Nozaki 4.457
DTASelect 4.685
Thurlkill 4.215
EMBOSS 4.279
Sillero 4.495
Patrickios 3.63
IPC_peptide 4.355
IPC2_peptide 4.482
IPC2.peptide.svr19 4.432
Protein with the highest isoelectric point:
>tr|A0A4D5ZMZ8|A0A4D5ZMZ8_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan43 OX=2558698 GN=JavanS43_0001 PE=4 SV=1
MM1 pKa = 7.38 IMKK4 pKa = 8.9 ITEE7 pKa = 4.08 VKK9 pKa = 10.56 KK10 pKa = 10.72 KK11 pKa = 10.15 DD12 pKa = 3.14 GTVIYY17 pKa = 9.92 RR18 pKa = 11.84 ASIYY22 pKa = 10.71 LGTDD26 pKa = 2.36 KK27 pKa = 10.8 VTGKK31 pKa = 10.64 KK32 pKa = 7.91 VTTKK36 pKa = 9.18 ITGRR40 pKa = 11.84 TKK42 pKa = 10.6 KK43 pKa = 9.66 EE44 pKa = 3.58 VRR46 pKa = 11.84 EE47 pKa = 4.0 KK48 pKa = 10.9 AKK50 pKa = 10.63 QEE52 pKa = 4.07 AIEE55 pKa = 4.6 FIKK58 pKa = 11.09 NGSTRR63 pKa = 11.84 FKK65 pKa = 9.65 ATSITSYY72 pKa = 11.23 QEE74 pKa = 4.84 LATLWWDD81 pKa = 3.74 SYY83 pKa = 11.1 KK84 pKa = 9.85 HH85 pKa = 4.21 TVKK88 pKa = 10.92 YY89 pKa = 8.4 NTQLATEE96 pKa = 4.37 KK97 pKa = 10.61 LLTVHH102 pKa = 6.77 VIPIFGAYY110 pKa = 9.53 KK111 pKa = 10.15 LDD113 pKa = 3.67 KK114 pKa = 10.12 LTTPLIQSIINKK126 pKa = 9.54 LADD129 pKa = 3.45 KK130 pKa = 9.75 TNKK133 pKa = 10.06 GEE135 pKa = 4.09 RR136 pKa = 11.84 KK137 pKa = 9.79 AYY139 pKa = 9.6 LHH141 pKa = 6.59 YY142 pKa = 11.09 DD143 pKa = 4.1 RR144 pKa = 11.84 IHH146 pKa = 6.67 ALNKK150 pKa = 10.16 RR151 pKa = 11.84 ILQYY155 pKa = 10.96 GVIMQAIPFNPARR168 pKa = 11.84 EE169 pKa = 4.24 VILPRR174 pKa = 11.84 NTKK177 pKa = 9.3 KK178 pKa = 10.99 ANTKK182 pKa = 9.27 RR183 pKa = 11.84 VKK185 pKa = 10.5 HH186 pKa = 5.63 FEE188 pKa = 3.77 NDD190 pKa = 3.02 EE191 pKa = 3.81 LRR193 pKa = 11.84 TFFNYY198 pKa = 10.44 LNNLDD203 pKa = 3.98 KK204 pKa = 11.36 NKK206 pKa = 9.8 YY207 pKa = 8.17 RR208 pKa = 11.84 YY209 pKa = 9.31 FYY211 pKa = 10.87 EE212 pKa = 3.8 VTLYY216 pKa = 11.0 KK217 pKa = 10.48 FLLATGCRR225 pKa = 11.84 INEE228 pKa = 3.8 ALALNWSDD236 pKa = 3.65 IDD238 pKa = 3.87 LDD240 pKa = 3.8 NAVVHH245 pKa = 5.5 ITKK248 pKa = 9.24 TLNYY252 pKa = 8.48 KK253 pKa = 9.4 QEE255 pKa = 4.1 INSPKK260 pKa = 10.23 SKK262 pKa = 10.48 SSYY265 pKa = 10.17 RR266 pKa = 11.84 DD267 pKa = 2.69 IDD269 pKa = 3.27 IDD271 pKa = 3.87 SRR273 pKa = 11.84 TVTMLKK279 pKa = 9.73 QYY281 pKa = 10.25 RR282 pKa = 11.84 RR283 pKa = 11.84 RR284 pKa = 11.84 QIQEE288 pKa = 2.96 AWKK291 pKa = 9.76 LGRR294 pKa = 11.84 SEE296 pKa = 4.29 TVVFSDD302 pKa = 6.41 FIHH305 pKa = 7.11 KK306 pKa = 10.24 YY307 pKa = 8.57 PNNRR311 pKa = 11.84 TLQTRR316 pKa = 11.84 LRR318 pKa = 11.84 THH320 pKa = 6.83 FKK322 pKa = 10.02 RR323 pKa = 11.84 ANVSNIGFHH332 pKa = 6.27 GFRR335 pKa = 11.84 HH336 pKa = 4.87 THH338 pKa = 6.65 ASLLLNTGIPYY349 pKa = 10.09 KK350 pKa = 10.14 EE351 pKa = 3.64 LQYY354 pKa = 11.41 RR355 pKa = 11.84 LGHH358 pKa = 5.67 STLSMTMDD366 pKa = 3.58 IYY368 pKa = 11.68 SHH370 pKa = 6.98 LSKK373 pKa = 11.0 EE374 pKa = 4.25 NAKK377 pKa = 10.34 KK378 pKa = 10.33 AVSFFEE384 pKa = 4.08 TAINSII390 pKa = 4.07
Molecular weight: 45.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.131
IPC2_protein 9.472
IPC_protein 9.443
Toseland 10.145
ProMoST 9.78
Dawson 10.321
Bjellqvist 9.97
Wikipedia 10.482
Rodwell 10.862
Grimsley 10.394
Solomon 10.335
Lehninger 10.306
Nozaki 10.116
DTASelect 9.97
Thurlkill 10.189
EMBOSS 10.54
Sillero 10.233
Patrickios 10.482
IPC_peptide 10.35
IPC2_peptide 8.463
IPC2.peptide.svr19 8.519
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
24
0
24
3614
38
500
150.6
17.53
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.313 ± 0.337
0.885 ± 0.179
5.755 ± 0.649
7.72 ± 0.521
4.842 ± 0.322
4.068 ± 0.423
1.771 ± 0.253
8.052 ± 0.472
9.408 ± 0.395
9.491 ± 0.405
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.158 ± 0.246
5.921 ± 0.323
3.21 ± 0.475
3.791 ± 0.31
4.842 ± 0.416
5.562 ± 0.549
6.309 ± 0.473
4.87 ± 0.315
0.968 ± 0.165
5.064 ± 0.478
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here