Chrysochromulina parva virophage Moe
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A499SF35|A0A499SF35_9VIRU S3H helicase OS=Chrysochromulina parva virophage Moe OX=2420055 GN=CpVVM_09 PE=4 SV=1
MM1 pKa = 7.44 TNYY4 pKa = 8.47 TNCISIEE11 pKa = 3.84 KK12 pKa = 10.26 ALYY15 pKa = 9.93 FDD17 pKa = 5.05 LFDD20 pKa = 6.38 DD21 pKa = 5.82 IDD23 pKa = 4.23 YY24 pKa = 10.81 KK25 pKa = 9.87 WLKK28 pKa = 10.59 LILDD32 pKa = 3.97 PSVDD36 pKa = 3.34 RR37 pKa = 11.84 EE38 pKa = 4.03 EE39 pKa = 4.59 YY40 pKa = 10.52 DD41 pKa = 3.61 FEE43 pKa = 5.28 VEE45 pKa = 4.31 IEE47 pKa = 4.61 DD48 pKa = 3.77 IPVEE52 pKa = 4.01 EE53 pKa = 4.43 VIEE56 pKa = 4.63 GFFKK60 pKa = 10.62 TSPEE64 pKa = 3.9 CRR66 pKa = 11.84 TTFTFDD72 pKa = 3.35 LKK74 pKa = 10.4 MAQTRR79 pKa = 11.84 PSFNANGYY87 pKa = 6.26 ITEE90 pKa = 4.74 YY91 pKa = 8.17 ITKK94 pKa = 8.83 TFTKK98 pKa = 9.63 TIDD101 pKa = 3.51 YY102 pKa = 10.0 GGSSAIAMTLCGMKK116 pKa = 10.5 DD117 pKa = 3.3 NEE119 pKa = 4.05 EE120 pKa = 4.28 DD121 pKa = 3.63 EE122 pKa = 4.66 EE123 pKa = 4.47 EE124 pKa = 4.39 EE125 pKa = 4.75 EE126 pKa = 4.61 EE127 pKa = 4.82 EE128 pKa = 4.45 EE129 pKa = 4.55 NNN131 pKa = 4.05
Molecular weight: 15.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.045
IPC2_protein 3.961
IPC_protein 3.897
Toseland 3.719
ProMoST 4.024
Dawson 3.846
Bjellqvist 4.012
Wikipedia 3.732
Rodwell 3.732
Grimsley 3.63
Solomon 3.846
Lehninger 3.795
Nozaki 3.961
DTASelect 4.101
Thurlkill 3.745
EMBOSS 3.745
Sillero 4.012
Patrickios 2.994
IPC_peptide 3.846
IPC2_peptide 3.986
IPC2.peptide.svr19 3.914
Protein with the highest isoelectric point:
>tr|A0A4P1LUG4|A0A4P1LUG4_9VIRU Uncharacterized protein OS=Chrysochromulina parva virophage Moe OX=2420055 GN=CpVVM_05 PE=4 SV=1
MM1 pKa = 7.78 PNCLDD6 pKa = 3.48 KK7 pKa = 11.2 TKK9 pKa = 10.99 LKK11 pKa = 11.22 ALMKK15 pKa = 9.83 GFKK18 pKa = 9.71 KK19 pKa = 10.64 RR20 pKa = 11.84 EE21 pKa = 3.98 VKK23 pKa = 10.46 EE24 pKa = 3.86 EE25 pKa = 4.07 EE26 pKa = 4.04 IIKK29 pKa = 10.43 PKK31 pKa = 10.81 AFVPAVTKK39 pKa = 11.04 GNTPTLAEE47 pKa = 4.03 KK48 pKa = 10.2 PINKK52 pKa = 9.59 KK53 pKa = 10.39 LRR55 pKa = 11.84 IKK57 pKa = 10.53 IKK59 pKa = 10.54 AA60 pKa = 3.28
Molecular weight: 6.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.013
IPC2_protein 9.399
IPC_protein 9.282
Toseland 10.613
ProMoST 9.984
Dawson 10.672
Bjellqvist 10.175
Wikipedia 10.716
Rodwell 11.55
Grimsley 10.672
Solomon 10.687
Lehninger 10.687
Nozaki 10.57
DTASelect 10.175
Thurlkill 10.57
EMBOSS 10.965
Sillero 10.57
Patrickios 11.301
IPC_peptide 10.701
IPC2_peptide 8.478
IPC2.peptide.svr19 8.353
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
23
0
23
6659
60
1745
289.5
32.86
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.848 ± 1.186
0.931 ± 0.3
6.427 ± 0.283
7.298 ± 0.487
3.724 ± 0.319
5.211 ± 0.509
1.472 ± 0.223
7.148 ± 0.652
8.335 ± 0.84
8.259 ± 0.436
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.463 ± 0.162
7.163 ± 0.587
4.821 ± 0.736
3.874 ± 0.542
4.37 ± 0.455
6.052 ± 0.514
6.187 ± 0.384
4.265 ± 0.279
0.721 ± 0.155
4.43 ± 0.373
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here