Enterococcus phage SAP6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Saphexavirus; Enterococcus virus SAP6

Average proteome isoelectric point is 5.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G1C4X8|G1C4X8_9CAUD SH3_15 domain-containing protein OS=Enterococcus phage SAP6 OX=1073766 PE=4 SV=1
MM1 pKa = 7.37KK2 pKa = 10.36LKK4 pKa = 10.95VGDD7 pKa = 3.85TVTVRR12 pKa = 11.84KK13 pKa = 9.98DD14 pKa = 4.35LEE16 pKa = 4.49TGWYY20 pKa = 9.16GAEE23 pKa = 3.76LVVPYY28 pKa = 10.07MLDD31 pKa = 3.33YY32 pKa = 11.12KK33 pKa = 11.21GITTNVKK40 pKa = 9.79KK41 pKa = 10.84VYY43 pKa = 10.56SDD45 pKa = 3.17GTLAIDD51 pKa = 3.21NGIWKK56 pKa = 7.53WTPEE60 pKa = 4.02MFEE63 pKa = 4.23EE64 pKa = 4.62TTSEE68 pKa = 3.53ITLEE72 pKa = 3.32IGYY75 pKa = 10.0RR76 pKa = 11.84YY77 pKa = 8.74LTDD80 pKa = 4.11TIVQTSHH87 pKa = 4.71QTVIDD92 pKa = 3.87DD93 pKa = 4.21DD94 pKa = 4.21YY95 pKa = 11.62AYY97 pKa = 10.5EE98 pKa = 4.09NACEE102 pKa = 3.92QFLEE106 pKa = 4.51EE107 pKa = 4.31FMKK110 pKa = 10.64DD111 pKa = 3.33YY112 pKa = 11.24GSVEE116 pKa = 4.33GIDD119 pKa = 2.97ICFVRR124 pKa = 11.84EE125 pKa = 4.05AFDD128 pKa = 3.51NAA130 pKa = 3.8

Molecular weight: 14.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G1C4W8|G1C4W8_9CAUD Uncharacterized protein OS=Enterococcus phage SAP6 OX=1073766 PE=4 SV=1
MM1 pKa = 7.64EE2 pKa = 5.74CLSDD6 pKa = 3.59SCRR9 pKa = 11.84NGLGKK14 pKa = 10.49LVGIAVGTPYY24 pKa = 10.53RR25 pKa = 11.84GFSGKK30 pKa = 10.13YY31 pKa = 9.1KK32 pKa = 10.47SEE34 pKa = 4.04AYY36 pKa = 10.28NVGFPKK42 pKa = 9.79TCQRR46 pKa = 11.84LTVARR51 pKa = 11.84PARR54 pKa = 11.84PRR56 pKa = 11.84KK57 pKa = 9.66SYY59 pKa = 9.99PLPFPKK65 pKa = 10.07TPARR69 pKa = 11.84RR70 pKa = 11.84GHH72 pKa = 6.47ACAPAPSSRR81 pKa = 11.84FATPCEE87 pKa = 4.17CFATLSRR94 pKa = 11.84HH95 pKa = 5.22AVRR98 pKa = 11.84HH99 pKa = 4.97AHH101 pKa = 5.97TSDD104 pKa = 3.38FTT106 pKa = 3.91

Molecular weight: 11.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

12404

100

1331

281.9

31.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.594 ± 0.575

0.637 ± 0.107

6.296 ± 0.177

8.715 ± 0.487

4.071 ± 0.235

6.312 ± 0.469

1.395 ± 0.134

6.135 ± 0.247

7.352 ± 0.298

8.11 ± 0.351

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.402 ± 0.207

5.063 ± 0.252

3.257 ± 0.292

3.813 ± 0.351

4.273 ± 0.233

6.143 ± 0.239

6.409 ± 0.432

6.861 ± 0.301

1.282 ± 0.129

3.878 ± 0.286
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski