Planococcus massiliensis
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3353 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A098EI80|A0A098EI80_9BACL Gamma-DL-glutamyl hydrolase OS=Planococcus massiliensis OX=1499687 GN=pgdS PE=3 SV=1
MM1 pKa = 7.72 EE2 pKa = 5.48 KK3 pKa = 10.69 LNYY6 pKa = 10.34 LILLLMAIFLVGCTDD21 pKa = 3.4 IEE23 pKa = 4.3 DD24 pKa = 3.93 TASVEE29 pKa = 4.23 EE30 pKa = 4.16 TDD32 pKa = 3.41 TDD34 pKa = 3.75 VKK36 pKa = 10.74 EE37 pKa = 4.16 VSTVEE42 pKa = 4.11 EE43 pKa = 4.33 EE44 pKa = 4.39 SSEE47 pKa = 4.21 EE48 pKa = 3.86 ATPEE52 pKa = 3.81 AVEE55 pKa = 4.22 EE56 pKa = 4.06 EE57 pKa = 4.59 LVAEE61 pKa = 4.21 EE62 pKa = 4.64 TPTEE66 pKa = 3.98 EE67 pKa = 3.76 TAAAPTDD74 pKa = 3.85 DD75 pKa = 5.73 LFAGYY80 pKa = 10.64 NLIEE84 pKa = 4.21 VDD86 pKa = 4.1 GGDD89 pKa = 3.57 LSGHH93 pKa = 6.18 RR94 pKa = 11.84 EE95 pKa = 3.97 ANVVVDD101 pKa = 3.59 IGFGDD106 pKa = 3.52 RR107 pKa = 11.84 EE108 pKa = 3.96 YY109 pKa = 10.09 WAFTNEE115 pKa = 3.57 HH116 pKa = 5.5 GQLVRR121 pKa = 11.84 VIASEE126 pKa = 5.68 IILQDD131 pKa = 4.01 DD132 pKa = 3.44 NTEE135 pKa = 4.19 PVTSSGRR142 pKa = 11.84 YY143 pKa = 8.54 YY144 pKa = 9.99 PDD146 pKa = 3.2 EE147 pKa = 4.48 AKK149 pKa = 10.97 VPGVEE154 pKa = 5.25 SPTLDD159 pKa = 3.7 EE160 pKa = 4.32 GHH162 pKa = 6.82 VIADD166 pKa = 3.61 SLGGVSNAYY175 pKa = 10.43 NITPQEE181 pKa = 4.15 STLNRR186 pKa = 11.84 YY187 pKa = 8.77 GDD189 pKa = 3.46 QAYY192 pKa = 9.09 MEE194 pKa = 4.31 DD195 pKa = 4.39 VIRR198 pKa = 11.84 KK199 pKa = 9.54 AGGAVDD205 pKa = 4.61 FEE207 pKa = 5.36 AIITYY212 pKa = 9.91 PDD214 pKa = 3.66 TEE216 pKa = 4.39 TQIPSHH222 pKa = 5.38 YY223 pKa = 10.19 QYY225 pKa = 11.01 TYY227 pKa = 9.12 TVNGNKK233 pKa = 9.31 VVDD236 pKa = 4.09 NYY238 pKa = 11.79 DD239 pKa = 3.3 NVNPDD244 pKa = 3.55 EE245 pKa = 4.5 VNEE248 pKa = 4.18 SLGLTGGDD256 pKa = 3.06 APEE259 pKa = 4.15 AAEE262 pKa = 4.89 AEE264 pKa = 4.58 PEE266 pKa = 4.27 SAPAAPAPAAGGDD279 pKa = 3.76 VSSVDD284 pKa = 3.28 TDD286 pKa = 3.62 GNGQVTIKK294 pKa = 9.56 EE295 pKa = 3.92 AKK297 pKa = 9.66 AAGFSMPIMSDD308 pKa = 2.56 HH309 pKa = 6.56 WLYY312 pKa = 10.6 PYY314 pKa = 10.18 MRR316 pKa = 11.84 DD317 pKa = 3.38 NDD319 pKa = 3.64 NDD321 pKa = 3.78 GMVGEE326 pKa = 4.62
Molecular weight: 35.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.71
IPC2_protein 3.719
IPC_protein 3.719
Toseland 3.516
ProMoST 3.846
Dawson 3.694
Bjellqvist 3.859
Wikipedia 3.592
Rodwell 3.554
Grimsley 3.427
Solomon 3.681
Lehninger 3.643
Nozaki 3.795
DTASelect 3.986
Thurlkill 3.554
EMBOSS 3.605
Sillero 3.834
Patrickios 1.265
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.755
Protein with the highest isoelectric point:
>tr|A0A098EHE5|A0A098EHE5_9BACL Laccase domain protein OS=Planococcus massiliensis OX=1499687 GN=BN1080_00141 PE=3 SV=1
MM1 pKa = 7.61 TLRR4 pKa = 11.84 TYY6 pKa = 10.57 QPNTRR11 pKa = 11.84 KK12 pKa = 9.66 HH13 pKa = 5.87 SKK15 pKa = 8.83 VHH17 pKa = 5.7 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MSTKK26 pKa = 9.71 NGRR29 pKa = 11.84 RR30 pKa = 11.84 VIAARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 8.8 GRR40 pKa = 11.84 KK41 pKa = 8.75 VLSAA45 pKa = 4.05
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 11.082
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.045
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3353
0
3353
986772
29
1528
294.3
32.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.417 ± 0.046
0.556 ± 0.01
5.108 ± 0.03
8.054 ± 0.053
4.62 ± 0.036
7.168 ± 0.043
2.002 ± 0.021
7.258 ± 0.04
6.209 ± 0.035
9.8 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.937 ± 0.021
3.834 ± 0.031
3.764 ± 0.02
3.655 ± 0.03
4.166 ± 0.032
5.905 ± 0.037
5.274 ± 0.029
6.975 ± 0.035
1.029 ± 0.017
3.267 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here