Amino acid dipepetide frequency for Nocardioides sp. Root614

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.704AlaAla: 18.704 ± 0.161	0.998AlaCys: 0.998 ± 0.027	8.522AlaAsp: 8.522 ± 0.08	7.795AlaGlu: 7.795 ± 0.105	3.64AlaPhe: 3.64 ± 0.055	11.874AlaGly: 11.874 ± 0.094	2.478AlaHis: 2.478 ± 0.043	5.133AlaIle: 5.133 ± 0.057	2.831AlaLys: 2.831 ± 0.055	13.091AlaLeu: 13.091 ± 0.111	2.725AlaMet: 2.725 ± 0.045	2.337AlaAsn: 2.337 ± 0.055	6.099AlaPro: 6.099 ± 0.082	3.474AlaGln: 3.474 ± 0.055	8.583AlaArg: 8.583 ± 0.111	6.684AlaSer: 6.684 ± 0.072	7.939AlaThr: 7.939 ± 0.081	11.471AlaVal: 11.471 ± 0.11	1.894AlaTrp: 1.894 ± 0.037	2.372AlaTyr: 2.372 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.943CysAla: 0.943 ± 0.022	0.084CysCys: 0.084 ± 0.007	0.468CysAsp: 0.468 ± 0.02	0.38CysGlu: 0.38 ± 0.015	0.225CysPhe: 0.225 ± 0.012	0.84CysGly: 0.84 ± 0.025	0.168CysHis: 0.168 ± 0.011	0.238CysIle: 0.238 ± 0.013	0.125CysLys: 0.125 ± 0.009	0.654CysLeu: 0.654 ± 0.02	0.118CysMet: 0.118 ± 0.007	0.17CysAsn: 0.17 ± 0.011	0.472CysPro: 0.472 ± 0.02	0.2CysGln: 0.2 ± 0.011	0.529CysArg: 0.529 ± 0.018	0.463CysSer: 0.463 ± 0.021	0.503CysThr: 0.503 ± 0.023	0.613CysVal: 0.613 ± 0.02	0.129CysTrp: 0.129 ± 0.016	0.151CysTyr: 0.151 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	8.075AspAla: 8.075 ± 0.084	0.442AspCys: 0.442 ± 0.017	4.774AspAsp: 4.774 ± 0.076	4.344AspGlu: 4.344 ± 0.064	1.799AspPhe: 1.799 ± 0.036	6.586AspGly: 6.586 ± 0.081	1.544AspHis: 1.544 ± 0.031	2.246AspIle: 2.246 ± 0.04	1.339AspLys: 1.339 ± 0.033	7.561AspLeu: 7.561 ± 0.077	0.834AspMet: 0.834 ± 0.023	1.202AspAsn: 1.202 ± 0.035	4.594AspPro: 4.594 ± 0.063	1.941AspGln: 1.941 ± 0.039	4.773AspArg: 4.773 ± 0.063	2.672AspSer: 2.672 ± 0.047	3.003AspThr: 3.003 ± 0.045	6.132AspVal: 6.132 ± 0.078	0.996AspTrp: 0.996 ± 0.027	1.197AspTyr: 1.197 ± 0.031	0.0AspXaa: 0.0 ± 0.0
Glu	6.881GluAla: 6.881 ± 0.089	0.352GluCys: 0.352 ± 0.014	3.036GluAsp: 3.036 ± 0.051	3.2GluGlu: 3.2 ± 0.058	1.659GluPhe: 1.659 ± 0.034	4.201GluGly: 4.201 ± 0.052	1.515GluHis: 1.515 ± 0.037	2.717GluIle: 2.717 ± 0.042	1.516GluLys: 1.516 ± 0.037	6.279GluLeu: 6.279 ± 0.077	1.022GluMet: 1.022 ± 0.027	1.116GluAsn: 1.116 ± 0.033	3.085GluPro: 3.085 ± 0.051	2.262GluGln: 2.262 ± 0.044	4.696GluArg: 4.696 ± 0.061	2.762GluSer: 2.762 ± 0.041	2.956GluThr: 2.956 ± 0.05	5.294GluVal: 5.294 ± 0.059	0.845GluTrp: 0.845 ± 0.023	1.075GluTyr: 1.075 ± 0.024	0.0GluXaa: 0.0 ± 0.0
Phe	3.808PheAla: 3.808 ± 0.052	0.264PheCys: 0.264 ± 0.012	2.242PheAsp: 2.242 ± 0.041	1.74PheGlu: 1.74 ± 0.038	0.929PhePhe: 0.929 ± 0.029	3.168PheGly: 3.168 ± 0.058	0.639PheHis: 0.639 ± 0.02	0.927PheIle: 0.927 ± 0.023	0.592PheLys: 0.592 ± 0.02	2.632PheLeu: 2.632 ± 0.043	0.428PheMet: 0.428 ± 0.018	0.692PheAsn: 0.692 ± 0.023	1.36PhePro: 1.36 ± 0.03	0.72PheGln: 0.72 ± 0.021	1.783PheArg: 1.783 ± 0.034	1.652PheSer: 1.652 ± 0.033	2.044PheThr: 2.044 ± 0.04	2.58PheVal: 2.58 ± 0.038	0.395PheTrp: 0.395 ± 0.019	0.633PheTyr: 0.633 ± 0.023	0.0PheXaa: 0.0 ± 0.0
Gly	10.233GlyAla: 10.233 ± 0.074	0.79GlyCys: 0.79 ± 0.021	5.752GlyAsp: 5.752 ± 0.074	4.874GlyGlu: 4.874 ± 0.057	3.038GlyPhe: 3.038 ± 0.045	8.157GlyGly: 8.157 ± 0.093	1.99GlyHis: 1.99 ± 0.031	4.004GlyIle: 4.004 ± 0.053	2.496GlyLys: 2.496 ± 0.047	9.254GlyLeu: 9.254 ± 0.088	1.81GlyMet: 1.81 ± 0.039	2.058GlyAsn: 2.058 ± 0.046	4.438GlyPro: 4.438 ± 0.058	2.761GlyGln: 2.761 ± 0.04	6.545GlyArg: 6.545 ± 0.074	5.499GlySer: 5.499 ± 0.067	5.918GlyThr: 5.918 ± 0.088	7.902GlyVal: 7.902 ± 0.078	1.639GlyTrp: 1.639 ± 0.032	2.171GlyTyr: 2.171 ± 0.042	0.001GlyXaa: 0.001 ± 0.001
His	2.406HisAla: 2.406 ± 0.041	0.19HisCys: 0.19 ± 0.01	1.412HisAsp: 1.412 ± 0.03	1.173HisGlu: 1.173 ± 0.028	0.671HisPhe: 0.671 ± 0.021	2.125HisGly: 2.125 ± 0.038	0.677HisHis: 0.677 ± 0.024	0.574HisIle: 0.574 ± 0.02	0.363HisLys: 0.363 ± 0.016	2.324HisLeu: 2.324 ± 0.045	0.278HisMet: 0.278 ± 0.014	0.385HisAsn: 0.385 ± 0.018	1.517HisPro: 1.517 ± 0.029	0.671HisGln: 0.671 ± 0.021	1.765HisArg: 1.765 ± 0.037	0.903HisSer: 0.903 ± 0.025	1.113HisThr: 1.113 ± 0.031	1.868HisVal: 1.868 ± 0.036	0.311HisTrp: 0.311 ± 0.015	0.482HisTyr: 0.482 ± 0.019	0.001HisXaa: 0.001 ± 0.001
Ile	5.769IleAla: 5.769 ± 0.07	0.319IleCys: 0.319 ± 0.015	3.273IleAsp: 3.273 ± 0.045	2.67IleGlu: 2.67 ± 0.046	0.921IlePhe: 0.921 ± 0.027	4.281IleGly: 4.281 ± 0.058	0.754IleHis: 0.754 ± 0.021	1.244IleIle: 1.244 ± 0.028	0.968IleLys: 0.968 ± 0.025	2.808IleLeu: 2.808 ± 0.047	0.491IleMet: 0.491 ± 0.018	1.047IleAsn: 1.047 ± 0.03	2.021IlePro: 2.021 ± 0.039	0.912IleGln: 0.912 ± 0.026	2.521IleArg: 2.521 ± 0.045	2.199IleSer: 2.199 ± 0.043	2.649IleThr: 2.649 ± 0.043	3.501IleVal: 3.501 ± 0.055	0.437IleTrp: 0.437 ± 0.018	0.688IleTyr: 0.688 ± 0.022	0.0IleXaa: 0.0 ± 0.0
Lys	3.034LysAla: 3.034 ± 0.055	0.117LysCys: 0.117 ± 0.008	1.373LysAsp: 1.373 ± 0.035	1.153LysGlu: 1.153 ± 0.027	0.598LysPhe: 0.598 ± 0.022	1.802LysGly: 1.802 ± 0.044	0.48LysHis: 0.48 ± 0.019	1.036LysIle: 1.036 ± 0.034	0.807LysLys: 0.807 ± 0.033	2.014LysLeu: 2.014 ± 0.04	0.451LysMet: 0.451 ± 0.018	0.571LysAsn: 0.571 ± 0.025	1.345LysPro: 1.345 ± 0.032	0.778LysGln: 0.778 ± 0.025	1.504LysArg: 1.504 ± 0.033	1.26LysSer: 1.26 ± 0.031	1.284LysThr: 1.284 ± 0.03	2.314LysVal: 2.314 ± 0.042	0.272LysTrp: 0.272 ± 0.013	0.53LysTyr: 0.53 ± 0.019	0.001LysXaa: 0.001 ± 0.001
Leu	14.484LeuAla: 14.484 ± 0.131	0.718LeuCys: 0.718 ± 0.022	6.999LeuAsp: 6.999 ± 0.079	5.37LeuGlu: 5.37 ± 0.069	2.534LeuPhe: 2.534 ± 0.045	9.314LeuGly: 9.314 ± 0.096	2.031LeuHis: 2.031 ± 0.039	3.446LeuIle: 3.446 ± 0.051	2.067LeuLys: 2.067 ± 0.036	10.318LeuLeu: 10.318 ± 0.134	1.617LeuMet: 1.617 ± 0.036	1.886LeuAsn: 1.886 ± 0.042	5.573LeuPro: 5.573 ± 0.062	2.458LeuGln: 2.458 ± 0.045	7.299LeuArg: 7.299 ± 0.083	5.429LeuSer: 5.429 ± 0.063	6.522LeuThr: 6.522 ± 0.07	9.832LeuVal: 9.832 ± 0.085	1.179LeuTrp: 1.179 ± 0.03	1.513LeuTyr: 1.513 ± 0.033	0.0LeuXaa: 0.0 ± 0.0
Met	2.362MetAla: 2.362 ± 0.038	0.134MetCys: 0.134 ± 0.009	0.859MetAsp: 0.859 ± 0.023	0.735MetGlu: 0.735 ± 0.022	0.503MetPhe: 0.503 ± 0.02	1.342MetGly: 1.342 ± 0.031	0.357MetHis: 0.357 ± 0.015	0.725MetIle: 0.725 ± 0.023	0.506MetLys: 0.506 ± 0.019	1.811MetLeu: 1.811 ± 0.031	0.347MetMet: 0.347 ± 0.016	0.439MetAsn: 0.439 ± 0.02	1.039MetPro: 1.039 ± 0.025	0.474MetGln: 0.474 ± 0.019	1.308MetArg: 1.308 ± 0.03	1.442MetSer: 1.442 ± 0.031	1.676MetThr: 1.676 ± 0.034	1.545MetVal: 1.545 ± 0.033	0.195MetTrp: 0.195 ± 0.011	0.304MetTyr: 0.304 ± 0.015	0.0MetXaa: 0.0 ± 0.0
Asn	2.496AsnAla: 2.496 ± 0.048	0.179AsnCys: 0.179 ± 0.011	1.361AsnAsp: 1.361 ± 0.031	1.053AsnGlu: 1.053 ± 0.028	0.572AsnPhe: 0.572 ± 0.019	2.086AsnGly: 2.086 ± 0.051	0.431AsnHis: 0.431 ± 0.016	0.785AsnIle: 0.785 ± 0.025	0.532AsnLys: 0.532 ± 0.024	2.05AsnLeu: 2.05 ± 0.043	0.32AsnMet: 0.32 ± 0.015	0.537AsnAsn: 0.537 ± 0.024	1.554AsnPro: 1.554 ± 0.033	0.655AsnGln: 0.655 ± 0.02	1.352AsnArg: 1.352 ± 0.029	0.947AsnSer: 0.947 ± 0.034	1.24AsnThr: 1.24 ± 0.038	1.728AsnVal: 1.728 ± 0.038	0.304AsnTrp: 0.304 ± 0.013	0.477AsnTyr: 0.477 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	7.098ProAla: 7.098 ± 0.091	0.321ProCys: 0.321 ± 0.015	4.346ProAsp: 4.346 ± 0.054	3.758ProGlu: 3.758 ± 0.056	1.562ProPhe: 1.562 ± 0.032	5.468ProGly: 5.468 ± 0.063	1.078ProHis: 1.078 ± 0.026	1.975ProIle: 1.975 ± 0.038	1.195ProLys: 1.195 ± 0.032	4.76ProLeu: 4.76 ± 0.055	1.034ProMet: 1.034 ± 0.025	1.075ProAsn: 1.075 ± 0.03	2.711ProPro: 2.711 ± 0.058	1.397ProGln: 1.397 ± 0.03	3.22ProArg: 3.22 ± 0.053	3.109ProSer: 3.109 ± 0.05	3.569ProThr: 3.569 ± 0.06	5.199ProVal: 5.199 ± 0.072	0.878ProTrp: 0.878 ± 0.024	1.151ProTyr: 1.151 ± 0.027	0.001ProXaa: 0.001 ± 0.001
Gln	3.533GlnAla: 3.533 ± 0.053	0.166GlnCys: 0.166 ± 0.012	1.349GlnAsp: 1.349 ± 0.031	1.276GlnGlu: 1.276 ± 0.032	0.85GlnPhe: 0.85 ± 0.026	2.135GlnGly: 2.135 ± 0.038	0.665GlnHis: 0.665 ± 0.02	1.274GlnIle: 1.274 ± 0.026	0.634GlnLys: 0.634 ± 0.021	3.098GlnLeu: 3.098 ± 0.046	0.601GlnMet: 0.601 ± 0.019	0.517GlnAsn: 0.517 ± 0.02	1.661GlnPro: 1.661 ± 0.036	1.157GlnGln: 1.157 ± 0.028	2.365GlnArg: 2.365 ± 0.047	1.324GlnSer: 1.324 ± 0.028	1.531GlnThr: 1.531 ± 0.032	2.995GlnVal: 2.995 ± 0.045	0.461GlnTrp: 0.461 ± 0.018	0.565GlnTyr: 0.565 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	8.458ArgAla: 8.458 ± 0.106	0.463ArgCys: 0.463 ± 0.022	4.26ArgAsp: 4.26 ± 0.059	4.036ArgGlu: 4.036 ± 0.053	2.307ArgPhe: 2.307 ± 0.035	5.223ArgGly: 5.223 ± 0.068	1.653ArgHis: 1.653 ± 0.038	3.381ArgIle: 3.381 ± 0.046	1.655ArgLys: 1.655 ± 0.038	7.44ArgLeu: 7.44 ± 0.089	1.646ArgMet: 1.646 ± 0.032	1.468ArgAsn: 1.468 ± 0.032	3.77ArgPro: 3.77 ± 0.057	2.065ArgGln: 2.065 ± 0.043	6.196ArgArg: 6.196 ± 0.087	4.106ArgSer: 4.106 ± 0.057	4.427ArgThr: 4.427 ± 0.054	5.682ArgVal: 5.682 ± 0.053	1.297ArgTrp: 1.297 ± 0.031	1.529ArgTyr: 1.529 ± 0.033	0.001ArgXaa: 0.001 ± 0.001
Ser	6.532SerAla: 6.532 ± 0.066	0.423SerCys: 0.423 ± 0.018	3.273SerAsp: 3.273 ± 0.053	2.612SerGlu: 2.612 ± 0.048	1.731SerPhe: 1.731 ± 0.035	5.933SerGly: 5.933 ± 0.077	1.032SerHis: 1.032 ± 0.026	2.16SerIle: 2.16 ± 0.038	1.168SerLys: 1.168 ± 0.028	5.102SerLeu: 5.102 ± 0.059	1.241SerMet: 1.241 ± 0.026	1.115SerAsn: 1.115 ± 0.032	3.049SerPro: 3.049 ± 0.047	1.372SerGln: 1.372 ± 0.027	3.678SerArg: 3.678 ± 0.053	3.375SerSer: 3.375 ± 0.06	3.739SerThr: 3.739 ± 0.053	4.582SerVal: 4.582 ± 0.059	0.878SerTrp: 0.878 ± 0.026	1.491SerTyr: 1.491 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	7.785ThrAla: 7.785 ± 0.086	0.532ThrCys: 0.532 ± 0.021	3.946ThrAsp: 3.946 ± 0.063	3.195ThrGlu: 3.195 ± 0.048	2.04ThrPhe: 2.04 ± 0.033	6.198ThrGly: 6.198 ± 0.084	1.193ThrHis: 1.193 ± 0.026	2.614ThrIle: 2.614 ± 0.048	1.325ThrLys: 1.325 ± 0.029	5.782ThrLeu: 5.782 ± 0.064	1.088ThrMet: 1.088 ± 0.026	1.335ThrAsn: 1.335 ± 0.038	3.836ThrPro: 3.836 ± 0.053	1.46ThrGln: 1.46 ± 0.027	3.777ThrArg: 3.777 ± 0.054	3.804ThrSer: 3.804 ± 0.057	4.372ThrThr: 4.372 ± 0.074	5.859ThrVal: 5.859 ± 0.074	1.027ThrTrp: 1.027 ± 0.025	1.452ThrTyr: 1.452 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	12.112ValAla: 12.112 ± 0.108	0.67ValCys: 0.67 ± 0.022	6.087ValAsp: 6.087 ± 0.076	5.417ValGlu: 5.417 ± 0.067	2.394ValPhe: 2.394 ± 0.041	7.485ValGly: 7.485 ± 0.07	1.843ValHis: 1.843 ± 0.035	3.835ValIle: 3.835 ± 0.048	1.884ValLys: 1.884 ± 0.037	9.749ValLeu: 9.749 ± 0.096	1.531ValMet: 1.531 ± 0.031	1.923ValAsn: 1.923 ± 0.038	5.12ValPro: 5.12 ± 0.062	2.299ValGln: 2.299 ± 0.044	6.508ValArg: 6.508 ± 0.071	4.775ValSer: 4.775 ± 0.06	6.052ValThr: 6.052 ± 0.083	9.667ValVal: 9.667 ± 0.113	1.107ValTrp: 1.107 ± 0.028	1.456ValTyr: 1.456 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.564TrpAla: 1.564 ± 0.034	0.133TrpCys: 0.133 ± 0.009	0.837TrpAsp: 0.837 ± 0.021	0.685TrpGlu: 0.685 ± 0.023	0.537TrpPhe: 0.537 ± 0.019	1.072TrpGly: 1.072 ± 0.03	0.349TrpHis: 0.349 ± 0.013	0.655TrpIle: 0.655 ± 0.021	0.35TrpLys: 0.35 ± 0.016	1.751TrpLeu: 1.751 ± 0.038	0.305TrpMet: 0.305 ± 0.014	0.387TrpAsn: 0.387 ± 0.015	0.695TrpPro: 0.695 ± 0.023	0.544TrpGln: 0.544 ± 0.018	1.191TrpArg: 1.191 ± 0.029	1.028TrpSer: 1.028 ± 0.027	0.997TrpThr: 0.997 ± 0.03	1.172TrpVal: 1.172 ± 0.03	0.347TrpTrp: 0.347 ± 0.016	0.305TrpTyr: 0.305 ± 0.013	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.363TyrAla: 2.363 ± 0.041	0.179TyrCys: 0.179 ± 0.01	1.885TyrAsp: 1.885 ± 0.037	1.001TyrGlu: 1.001 ± 0.026	0.718TyrPhe: 0.718 ± 0.023	2.036TyrGly: 2.036 ± 0.038	0.331TyrHis: 0.331 ± 0.015	0.503TyrIle: 0.503 ± 0.017	0.371TyrLys: 0.371 ± 0.016	2.112TyrLeu: 2.112 ± 0.041	0.218TyrMet: 0.218 ± 0.011	0.398TyrAsn: 0.398 ± 0.016	0.967TyrPro: 0.967 ± 0.03	0.556TyrGln: 0.556 ± 0.018	1.471TyrArg: 1.471 ± 0.032	1.055TyrSer: 1.055 ± 0.026	1.082TyrThr: 1.082 ± 0.029	1.979TyrVal: 1.979 ± 0.039	0.314TyrTrp: 0.314 ± 0.015	0.457TyrTyr: 0.457 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4780 proteins (1577883 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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