Micromonospora nigra

Taxonomy: cellular organisms;

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5321 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1C6T2Z7|A0A1C6T2Z7_9ACTN ATP-grasp domain-containing protein OS=Micromonospora nigra OX=145857 GN=GA0070616_5309 PE=4 SV=1
MM1 pKa = 7.6GGAQVADD8 pKa = 3.67VATDD12 pKa = 3.52QLQVWVDD19 pKa = 3.37QDD21 pKa = 3.81LCTGDD26 pKa = 3.99GLCVQYY32 pKa = 10.98APEE35 pKa = 4.12VFEE38 pKa = 5.1FDD40 pKa = 3.14IDD42 pKa = 3.39GLAYY46 pKa = 10.67VKK48 pKa = 10.87GPDD51 pKa = 3.68GEE53 pKa = 5.17LVQTPGGRR61 pKa = 11.84VGVPEE66 pKa = 4.1HH67 pKa = 6.55LRR69 pKa = 11.84LEE71 pKa = 4.75VIDD74 pKa = 4.27SAKK77 pKa = 10.05EE78 pKa = 3.91CPGEE82 pKa = 4.37CIHH85 pKa = 6.31VVRR88 pKa = 11.84GSDD91 pKa = 3.52DD92 pKa = 3.83VEE94 pKa = 4.44VAGPAAEE101 pKa = 4.19EE102 pKa = 4.53GG103 pKa = 3.73

Molecular weight: 10.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1C6SMQ9|A0A1C6SMQ9_9ACTN Ribonuclease J OS=Micromonospora nigra OX=145857 GN=rnj PE=3 SV=1
MM1 pKa = 7.16SRR3 pKa = 11.84VVSAGGVGRR12 pKa = 11.84VVSAGGVGRR21 pKa = 11.84VVSAGGVGRR30 pKa = 11.84VVSAGGVGRR39 pKa = 11.84VVSAGGVGRR48 pKa = 11.84VVSAGGVGRR57 pKa = 11.84VALAGGASRR66 pKa = 11.84AVAGG70 pKa = 4.11

Molecular weight: 6.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5321

0

5321

1856018

39

7874

348.8

37.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.236 ± 0.047

0.769 ± 0.009

6.243 ± 0.028

4.961 ± 0.03

2.531 ± 0.015

9.435 ± 0.031

2.223 ± 0.018

2.829 ± 0.022

1.444 ± 0.025

10.459 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.539 ± 0.013

1.675 ± 0.022

6.53 ± 0.033

2.698 ± 0.017

8.719 ± 0.035

4.538 ± 0.029

6.268 ± 0.031

9.34 ± 0.033

1.601 ± 0.013

1.961 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski