Staphylococcus phage CNPx

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Rockefellervirus; Staphylococcus virus CNPx

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A141VTP1|A0A141VTP1_9CAUD Uncharacterized protein OS=Staphylococcus phage CNPx OX=1792269 PE=4 SV=1
MM1 pKa = 7.52NGRR4 pKa = 11.84SDD6 pKa = 3.59EE7 pKa = 4.24MKK9 pKa = 10.64VVYY12 pKa = 9.59LWKK15 pKa = 10.26NGQAIIVHH23 pKa = 6.01KK24 pKa = 10.76NEE26 pKa = 3.61EE27 pKa = 4.13DD28 pKa = 3.72EE29 pKa = 4.52YY30 pKa = 11.45VYY32 pKa = 10.65PDD34 pKa = 3.75EE35 pKa = 5.4KK36 pKa = 9.76WTEE39 pKa = 3.87NQPPQGIILPCYY51 pKa = 9.8YY52 pKa = 10.33DD53 pKa = 3.45GKK55 pKa = 10.21QWVGQTQDD63 pKa = 3.78EE64 pKa = 4.6LEE66 pKa = 4.26KK67 pKa = 10.23MLPEE71 pKa = 4.0VEE73 pKa = 4.68IPVDD77 pKa = 4.01DD78 pKa = 4.81KK79 pKa = 11.74DD80 pKa = 3.4IAIAKK85 pKa = 7.34LTSLVVDD92 pKa = 4.38LQEE95 pKa = 4.31EE96 pKa = 4.77VMSLKK101 pKa = 10.75QNIALITEE109 pKa = 4.14EE110 pKa = 4.46QANQKK115 pKa = 10.36LGEE118 pKa = 4.04AA119 pKa = 4.5

Molecular weight: 13.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A141VTN5|A0A141VTN5_9CAUD Tail length tape-measure protein OS=Staphylococcus phage CNPx OX=1792269 PE=4 SV=1
MM1 pKa = 7.54SLTPNEE7 pKa = 3.89WKK9 pKa = 10.31DD10 pKa = 3.28WVVGRR15 pKa = 11.84RR16 pKa = 11.84LALLDD21 pKa = 3.68EE22 pKa = 4.38QEE24 pKa = 4.25NLLFGAQANGLVQAGKK40 pKa = 9.58SLKK43 pKa = 10.18RR44 pKa = 11.84LQKK47 pKa = 8.59QLEE50 pKa = 3.97RR51 pKa = 11.84ARR53 pKa = 11.84YY54 pKa = 6.86EE55 pKa = 3.64VRR57 pKa = 11.84GQSEE61 pKa = 4.38EE62 pKa = 4.06YY63 pKa = 10.73EE64 pKa = 4.03RR65 pKa = 11.84MKK67 pKa = 10.59EE68 pKa = 4.04RR69 pKa = 11.84KK70 pKa = 8.73LAHH73 pKa = 6.28NKK75 pKa = 10.13RR76 pKa = 11.84IRR78 pKa = 11.84NVQKK82 pKa = 9.93QGTRR86 pKa = 11.84RR87 pKa = 11.84FMNSLRR93 pKa = 11.84NTSQKK98 pKa = 11.01GGG100 pKa = 3.49

Molecular weight: 11.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

13714

37

1034

198.8

22.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.389 ± 0.448

0.591 ± 0.108

6.242 ± 0.364

7.452 ± 0.52

3.755 ± 0.166

5.987 ± 0.374

1.772 ± 0.149

7.321 ± 0.213

9.355 ± 0.395

7.678 ± 0.281

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.501 ± 0.187

7.007 ± 0.378

2.858 ± 0.182

4.076 ± 0.25

4.448 ± 0.248

5.586 ± 0.28

6.016 ± 0.233

6.249 ± 0.223

1.305 ± 0.14

4.412 ± 0.363
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski