Wuhan spider virus 7
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KIK4|A0A1L3KIK4_9VIRU RNA-dependent RNA polymerase OS=Wuhan spider virus 7 OX=1923756 PE=4 SV=1
MM1 pKa = 7.42 NEE3 pKa = 3.73 SLFAKK8 pKa = 10.13 RR9 pKa = 11.84 IEE11 pKa = 4.15 EE12 pKa = 4.17 VEE14 pKa = 4.02 KK15 pKa = 11.09 SILNLADD22 pKa = 3.14 WVGQSHH28 pKa = 6.65 KK29 pKa = 10.53 RR30 pKa = 11.84 PAGSTGLEE38 pKa = 3.72 RR39 pKa = 11.84 RR40 pKa = 11.84 ISEE43 pKa = 4.04 IFGCPYY49 pKa = 9.75 HH50 pKa = 6.85 GPVMVLSLPDD60 pKa = 3.39 RR61 pKa = 11.84 LLEE64 pKa = 4.17 DD65 pKa = 3.94 FRR67 pKa = 11.84 EE68 pKa = 4.08 EE69 pKa = 4.31 SFDD72 pKa = 3.88 FQSLQNRR79 pKa = 11.84 MTSTEE84 pKa = 4.21 LYY86 pKa = 10.11 IEE88 pKa = 4.58 AVTHH92 pKa = 6.11 RR93 pKa = 11.84 FIHH96 pKa = 6.27 AWTLAKK102 pKa = 10.13 TMTLAYY108 pKa = 9.46 WDD110 pKa = 3.61 ILEE113 pKa = 5.02 LYY115 pKa = 9.99 GFEE118 pKa = 5.85 ISDD121 pKa = 3.05 TWRR124 pKa = 11.84 CYY126 pKa = 10.42 RR127 pKa = 11.84 EE128 pKa = 3.62 LSSRR132 pKa = 11.84 IFSFGILNLGEE143 pKa = 5.32 AYY145 pKa = 10.43 LKK147 pKa = 10.74 HH148 pKa = 4.76 QTAFLLARR156 pKa = 11.84 CLEE159 pKa = 4.33 NEE161 pKa = 4.07 PPPRR165 pKa = 11.84 PSWFPWCEE173 pKa = 3.66 RR174 pKa = 11.84 EE175 pKa = 4.57 GYY177 pKa = 10.62 LLGGPVMRR185 pKa = 11.84 RR186 pKa = 11.84 LWCRR190 pKa = 11.84 LVLRR194 pKa = 11.84 RR195 pKa = 11.84 HH196 pKa = 5.96 KK197 pKa = 10.64 RR198 pKa = 11.84 DD199 pKa = 2.82 ISLAYY204 pKa = 10.11 SLYY207 pKa = 9.2 QAKK210 pKa = 9.68 KK211 pKa = 8.14 VAPEE215 pKa = 3.98 ISPLLVNEE223 pKa = 4.6 AMQKK227 pKa = 10.3 NLDD230 pKa = 3.83 LLTSEE235 pKa = 5.72 RR236 pKa = 11.84 EE237 pKa = 4.0 DD238 pKa = 5.58 DD239 pKa = 4.09 DD240 pKa = 6.04 VEE242 pKa = 4.13 DD243 pKa = 3.96 LKK245 pKa = 11.33 YY246 pKa = 10.16 QVRR249 pKa = 11.84 RR250 pKa = 11.84 TVRR253 pKa = 11.84 EE254 pKa = 3.42 ISKK257 pKa = 8.49 ITGPGLSFKK266 pKa = 10.21 TSPFPSLSACFEE278 pKa = 4.12 RR279 pKa = 11.84 PRR281 pKa = 11.84 SKK283 pKa = 10.93 GGALEE288 pKa = 4.07 EE289 pKa = 4.01 LLPRR293 pKa = 11.84 DD294 pKa = 3.46 GSKK297 pKa = 10.09 ILAFKK302 pKa = 10.35 EE303 pKa = 3.42 LLGYY307 pKa = 9.91 VIHH310 pKa = 6.76 RR311 pKa = 11.84 TQQLPIYY318 pKa = 8.21 GIPYY322 pKa = 10.37 DD323 pKa = 3.96 EE324 pKa = 5.55 DD325 pKa = 5.99 RR326 pKa = 11.84 ITFPFPIHH334 pKa = 6.91 RR335 pKa = 11.84 NGEE338 pKa = 3.94 SGYY341 pKa = 8.79 TRR343 pKa = 11.84 LHH345 pKa = 6.4 KK346 pKa = 10.71 DD347 pKa = 3.1 LMVRR351 pKa = 11.84 RR352 pKa = 11.84 QAITEE357 pKa = 4.03 PFKK360 pKa = 11.37 VRR362 pKa = 11.84 VVSMGSAEE370 pKa = 4.39 HH371 pKa = 5.45 YY372 pKa = 9.36 QALKK376 pKa = 10.71 GLQSEE381 pKa = 4.36 LWGFLRR387 pKa = 11.84 KK388 pKa = 10.02 SPVFDD393 pKa = 3.19 LTGRR397 pKa = 11.84 PSEE400 pKa = 4.35 EE401 pKa = 3.91 GDD403 pKa = 3.53 VASICRR409 pKa = 11.84 WVGEE413 pKa = 4.49 FVPNHH418 pKa = 5.14 YY419 pKa = 11.65 VMMVSGDD426 pKa = 3.68 YY427 pKa = 11.1 SSATDD432 pKa = 3.55 NLHH435 pKa = 6.85 PEE437 pKa = 3.88 LSEE440 pKa = 4.05 ACFDD444 pKa = 3.97 EE445 pKa = 4.76 LCNVLDD451 pKa = 3.99 IPWPIRR457 pKa = 11.84 SLGFQGLTRR466 pKa = 11.84 HH467 pKa = 6.42 TIVDD471 pKa = 3.73 DD472 pKa = 4.54 DD473 pKa = 5.09 GSVHH477 pKa = 5.54 RR478 pKa = 11.84 QCWGQLMGSPISFPILCLVNCAVTRR503 pKa = 11.84 FQMEE507 pKa = 4.18 KK508 pKa = 10.42 EE509 pKa = 3.95 FGLFIPLSYY518 pKa = 9.78 PDD520 pKa = 4.54 PSFSPLKK527 pKa = 10.76 INGDD531 pKa = 4.23 DD532 pKa = 3.69 ILFPLPPGGYY542 pKa = 8.94 QNWCEE547 pKa = 4.05 TVTRR551 pKa = 11.84 AGLAPSVGKK560 pKa = 10.3 NYY562 pKa = 9.69 VSRR565 pKa = 11.84 RR566 pKa = 11.84 VAVINSEE573 pKa = 4.58 LYY575 pKa = 10.67 DD576 pKa = 5.26 LSPDD580 pKa = 3.06 WDD582 pKa = 3.81 RR583 pKa = 11.84 GHH585 pKa = 6.79 EE586 pKa = 4.14 EE587 pKa = 4.01 VTRR590 pKa = 11.84 IPYY593 pKa = 10.1 INLGLLKK600 pKa = 10.3 YY601 pKa = 10.23 VQDD604 pKa = 3.71 HH605 pKa = 5.99 GRR607 pKa = 11.84 GDD609 pKa = 3.39 EE610 pKa = 4.02 AQAFVEE616 pKa = 4.71 DD617 pKa = 4.26 NFVSTMRR624 pKa = 11.84 EE625 pKa = 3.56 RR626 pKa = 11.84 LITATRR632 pKa = 11.84 GWDD635 pKa = 3.29 NDD637 pKa = 3.68 TADD640 pKa = 4.44 RR641 pKa = 11.84 LVSYY645 pKa = 9.18 CLRR648 pKa = 11.84 SNHH651 pKa = 5.88 GLLSRR656 pKa = 11.84 IPPVSWWVAEE666 pKa = 4.34 EE667 pKa = 3.97 YY668 pKa = 10.97 GGLGLPSFRR677 pKa = 11.84 EE678 pKa = 3.92 VEE680 pKa = 4.1 IAEE683 pKa = 4.25 HH684 pKa = 5.92 HH685 pKa = 6.77 LKK687 pKa = 10.49 LAAAFLMVDD696 pKa = 3.48 QRR698 pKa = 11.84 LIWSMRR704 pKa = 11.84 HH705 pKa = 5.41 PRR707 pKa = 11.84 SVCQLTTFSQEE718 pKa = 3.54 TEE720 pKa = 4.08 RR721 pKa = 11.84 YY722 pKa = 8.58 IGQIYY727 pKa = 9.67 DD728 pKa = 3.36 QLEE731 pKa = 4.28 IPWMVINPEE740 pKa = 4.25 MEE742 pKa = 5.0 DD743 pKa = 3.84 DD744 pKa = 3.83 CCKK747 pKa = 10.02 PAEE750 pKa = 4.28 IYY752 pKa = 10.59 VPQILRR758 pKa = 11.84 GYY760 pKa = 7.72 TCLGAGRR767 pKa = 11.84 EE768 pKa = 4.16 LTGDD772 pKa = 3.65 GFLKK776 pKa = 10.34 RR777 pKa = 11.84 WAYY780 pKa = 10.52 VYY782 pKa = 10.36 NRR784 pKa = 11.84 WMKK787 pKa = 9.86 ILRR790 pKa = 11.84 RR791 pKa = 11.84 NWLVFKK797 pKa = 10.63 PMNKK801 pKa = 9.52 KK802 pKa = 10.28 KK803 pKa = 10.44 SLEE806 pKa = 3.84 RR807 pKa = 11.84 TGRR810 pKa = 11.84 FWIQAIPKK818 pKa = 9.64 ASYY821 pKa = 9.83 HH822 pKa = 5.32 LHH824 pKa = 6.64 HH825 pKa = 6.63 NHH827 pKa = 6.58 SGPIKK832 pKa = 9.8 PSEE835 pKa = 4.08 IQDD838 pKa = 3.34 VDD840 pKa = 3.1 ISLSS844 pKa = 3.42
Molecular weight: 97.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.976
IPC2_protein 5.97
IPC_protein 6.033
Toseland 6.351
ProMoST 6.415
Dawson 6.338
Bjellqvist 6.351
Wikipedia 6.351
Rodwell 6.338
Grimsley 6.491
Solomon 6.338
Lehninger 6.338
Nozaki 6.62
DTASelect 6.795
Thurlkill 6.825
EMBOSS 6.781
Sillero 6.722
Patrickios 4.139
IPC_peptide 6.364
IPC2_peptide 6.678
IPC2.peptide.svr19 6.638
Protein with the highest isoelectric point:
>tr|A0A1L3KIK4|A0A1L3KIK4_9VIRU RNA-dependent RNA polymerase OS=Wuhan spider virus 7 OX=1923756 PE=4 SV=1
MM1 pKa = 7.42 NEE3 pKa = 3.73 SLFAKK8 pKa = 10.13 RR9 pKa = 11.84 IEE11 pKa = 4.15 EE12 pKa = 4.17 VEE14 pKa = 4.02 KK15 pKa = 11.09 SILNLADD22 pKa = 3.14 WVGQSHH28 pKa = 6.65 KK29 pKa = 10.53 RR30 pKa = 11.84 PAGSTGLEE38 pKa = 3.72 RR39 pKa = 11.84 RR40 pKa = 11.84 ISEE43 pKa = 4.04 IFGCPYY49 pKa = 9.75 HH50 pKa = 6.85 GPVMVLSLPDD60 pKa = 3.39 RR61 pKa = 11.84 LLEE64 pKa = 4.17 DD65 pKa = 3.94 FRR67 pKa = 11.84 EE68 pKa = 4.08 EE69 pKa = 4.31 SFDD72 pKa = 3.88 FQSLQNRR79 pKa = 11.84 MTSTEE84 pKa = 4.21 LYY86 pKa = 10.11 IEE88 pKa = 4.58 AVTHH92 pKa = 6.11 RR93 pKa = 11.84 FIHH96 pKa = 6.27 AWTLAKK102 pKa = 10.13 TMTLAYY108 pKa = 9.46 WDD110 pKa = 3.61 ILEE113 pKa = 5.02 LYY115 pKa = 9.99 GFEE118 pKa = 5.85 ISDD121 pKa = 3.05 TWRR124 pKa = 11.84 CYY126 pKa = 10.42 RR127 pKa = 11.84 EE128 pKa = 3.62 LSSRR132 pKa = 11.84 IFSFGILNLGEE143 pKa = 5.32 AYY145 pKa = 10.43 LKK147 pKa = 10.74 HH148 pKa = 4.76 QTAFLLARR156 pKa = 11.84 CLEE159 pKa = 4.33 NEE161 pKa = 4.07 PPPRR165 pKa = 11.84 PSWFPWCEE173 pKa = 3.66 RR174 pKa = 11.84 EE175 pKa = 4.57 GYY177 pKa = 10.62 LLGGPVMRR185 pKa = 11.84 RR186 pKa = 11.84 LWCRR190 pKa = 11.84 LVLRR194 pKa = 11.84 RR195 pKa = 11.84 HH196 pKa = 5.96 KK197 pKa = 10.64 RR198 pKa = 11.84 DD199 pKa = 2.82 ISLAYY204 pKa = 10.11 SLYY207 pKa = 9.2 QAKK210 pKa = 9.68 KK211 pKa = 8.14 VAPEE215 pKa = 3.98 ISPLLVNEE223 pKa = 4.6 AMQKK227 pKa = 10.3 NLDD230 pKa = 3.83 LLTSEE235 pKa = 5.72 RR236 pKa = 11.84 EE237 pKa = 4.0 DD238 pKa = 5.58 DD239 pKa = 4.09 DD240 pKa = 6.04 VEE242 pKa = 4.13 DD243 pKa = 3.96 LKK245 pKa = 11.33 YY246 pKa = 10.16 QVRR249 pKa = 11.84 RR250 pKa = 11.84 TVRR253 pKa = 11.84 EE254 pKa = 3.42 ISKK257 pKa = 8.49 ITGPGLSFKK266 pKa = 10.21 TSPFPSLSACFEE278 pKa = 4.12 RR279 pKa = 11.84 PRR281 pKa = 11.84 SKK283 pKa = 10.93 GGALEE288 pKa = 4.07 EE289 pKa = 4.01 LLPRR293 pKa = 11.84 DD294 pKa = 3.46 GSKK297 pKa = 10.09 ILAFKK302 pKa = 10.35 EE303 pKa = 3.42 LLGYY307 pKa = 9.91 VIHH310 pKa = 6.76 RR311 pKa = 11.84 TQQLPIYY318 pKa = 8.21 GIPYY322 pKa = 10.37 DD323 pKa = 3.96 EE324 pKa = 5.55 DD325 pKa = 5.99 RR326 pKa = 11.84 ITFPFPIHH334 pKa = 6.91 RR335 pKa = 11.84 NGEE338 pKa = 3.94 SGYY341 pKa = 8.79 TRR343 pKa = 11.84 LHH345 pKa = 6.4 KK346 pKa = 10.71 DD347 pKa = 3.1 LMVRR351 pKa = 11.84 RR352 pKa = 11.84 QAITEE357 pKa = 4.03 PFKK360 pKa = 11.37 VRR362 pKa = 11.84 VVSMGSAEE370 pKa = 4.39 HH371 pKa = 5.45 YY372 pKa = 9.36 QALKK376 pKa = 10.71 GLQSEE381 pKa = 4.36 LWGFLRR387 pKa = 11.84 KK388 pKa = 10.02 SPVFDD393 pKa = 3.19 LTGRR397 pKa = 11.84 PSEE400 pKa = 4.35 EE401 pKa = 3.91 GDD403 pKa = 3.53 VASICRR409 pKa = 11.84 WVGEE413 pKa = 4.49 FVPNHH418 pKa = 5.14 YY419 pKa = 11.65 VMMVSGDD426 pKa = 3.68 YY427 pKa = 11.1 SSATDD432 pKa = 3.55 NLHH435 pKa = 6.85 PEE437 pKa = 3.88 LSEE440 pKa = 4.05 ACFDD444 pKa = 3.97 EE445 pKa = 4.76 LCNVLDD451 pKa = 3.99 IPWPIRR457 pKa = 11.84 SLGFQGLTRR466 pKa = 11.84 HH467 pKa = 6.42 TIVDD471 pKa = 3.73 DD472 pKa = 4.54 DD473 pKa = 5.09 GSVHH477 pKa = 5.54 RR478 pKa = 11.84 QCWGQLMGSPISFPILCLVNCAVTRR503 pKa = 11.84 FQMEE507 pKa = 4.18 KK508 pKa = 10.42 EE509 pKa = 3.95 FGLFIPLSYY518 pKa = 9.78 PDD520 pKa = 4.54 PSFSPLKK527 pKa = 10.76 INGDD531 pKa = 4.23 DD532 pKa = 3.69 ILFPLPPGGYY542 pKa = 8.94 QNWCEE547 pKa = 4.05 TVTRR551 pKa = 11.84 AGLAPSVGKK560 pKa = 10.3 NYY562 pKa = 9.69 VSRR565 pKa = 11.84 RR566 pKa = 11.84 VAVINSEE573 pKa = 4.58 LYY575 pKa = 10.67 DD576 pKa = 5.26 LSPDD580 pKa = 3.06 WDD582 pKa = 3.81 RR583 pKa = 11.84 GHH585 pKa = 6.79 EE586 pKa = 4.14 EE587 pKa = 4.01 VTRR590 pKa = 11.84 IPYY593 pKa = 10.1 INLGLLKK600 pKa = 10.3 YY601 pKa = 10.23 VQDD604 pKa = 3.71 HH605 pKa = 5.99 GRR607 pKa = 11.84 GDD609 pKa = 3.39 EE610 pKa = 4.02 AQAFVEE616 pKa = 4.71 DD617 pKa = 4.26 NFVSTMRR624 pKa = 11.84 EE625 pKa = 3.56 RR626 pKa = 11.84 LITATRR632 pKa = 11.84 GWDD635 pKa = 3.29 NDD637 pKa = 3.68 TADD640 pKa = 4.44 RR641 pKa = 11.84 LVSYY645 pKa = 9.18 CLRR648 pKa = 11.84 SNHH651 pKa = 5.88 GLLSRR656 pKa = 11.84 IPPVSWWVAEE666 pKa = 4.34 EE667 pKa = 3.97 YY668 pKa = 10.97 GGLGLPSFRR677 pKa = 11.84 EE678 pKa = 3.92 VEE680 pKa = 4.1 IAEE683 pKa = 4.25 HH684 pKa = 5.92 HH685 pKa = 6.77 LKK687 pKa = 10.49 LAAAFLMVDD696 pKa = 3.48 QRR698 pKa = 11.84 LIWSMRR704 pKa = 11.84 HH705 pKa = 5.41 PRR707 pKa = 11.84 SVCQLTTFSQEE718 pKa = 3.54 TEE720 pKa = 4.08 RR721 pKa = 11.84 YY722 pKa = 8.58 IGQIYY727 pKa = 9.67 DD728 pKa = 3.36 QLEE731 pKa = 4.28 IPWMVINPEE740 pKa = 4.25 MEE742 pKa = 5.0 DD743 pKa = 3.84 DD744 pKa = 3.83 CCKK747 pKa = 10.02 PAEE750 pKa = 4.28 IYY752 pKa = 10.59 VPQILRR758 pKa = 11.84 GYY760 pKa = 7.72 TCLGAGRR767 pKa = 11.84 EE768 pKa = 4.16 LTGDD772 pKa = 3.65 GFLKK776 pKa = 10.34 RR777 pKa = 11.84 WAYY780 pKa = 10.52 VYY782 pKa = 10.36 NRR784 pKa = 11.84 WMKK787 pKa = 9.86 ILRR790 pKa = 11.84 RR791 pKa = 11.84 NWLVFKK797 pKa = 10.63 PMNKK801 pKa = 9.52 KK802 pKa = 10.28 KK803 pKa = 10.44 SLEE806 pKa = 3.84 RR807 pKa = 11.84 TGRR810 pKa = 11.84 FWIQAIPKK818 pKa = 9.64 ASYY821 pKa = 9.83 HH822 pKa = 5.32 LHH824 pKa = 6.64 HH825 pKa = 6.63 NHH827 pKa = 6.58 SGPIKK832 pKa = 9.8 PSEE835 pKa = 4.08 IQDD838 pKa = 3.34 VDD840 pKa = 3.1 ISLSS844 pKa = 3.42
Molecular weight: 97.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.976
IPC2_protein 5.97
IPC_protein 6.033
Toseland 6.351
ProMoST 6.415
Dawson 6.338
Bjellqvist 6.351
Wikipedia 6.351
Rodwell 6.338
Grimsley 6.491
Solomon 6.338
Lehninger 6.338
Nozaki 6.62
DTASelect 6.795
Thurlkill 6.825
EMBOSS 6.781
Sillero 6.722
Patrickios 4.139
IPC_peptide 6.364
IPC2_peptide 6.678
IPC2.peptide.svr19 6.638
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
844
844
844
844.0
97.22
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.858 ± 0.0
2.133 ± 0.0
5.213 ± 0.0
7.464 ± 0.0
4.384 ± 0.0
6.517 ± 0.0
2.844 ± 0.0
5.806 ± 0.0
3.91 ± 0.0
10.9 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.251 ± 0.0
2.962 ± 0.0
6.161 ± 0.0
3.199 ± 0.0
7.82 ± 0.0
7.346 ± 0.0
4.147 ± 0.0
5.687 ± 0.0
2.607 ± 0.0
3.791 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here