Cowpea golden mosaic virus-[Nigeria]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; unclassified Begomovirus

Average proteome isoelectric point is 7.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O55380|O55380_9GEMI C4 protein OS=Cowpea golden mosaic virus-[Nigeria] OX=223260 GN=c4 PE=3 SV=1
MM1 pKa = 7.6GNLICTYY8 pKa = 9.82CYY10 pKa = 10.31NSKK13 pKa = 10.27VSSRR17 pKa = 11.84SQIQDD22 pKa = 3.07CSTLSLEE29 pKa = 4.14TDD31 pKa = 4.25LPCFIQTFRR40 pKa = 11.84EE41 pKa = 4.04QNRR44 pKa = 11.84APTSSPISRR53 pKa = 11.84RR54 pKa = 11.84MEE56 pKa = 4.34TPSHH60 pKa = 5.99GANSRR65 pKa = 11.84SMEE68 pKa = 4.3DD69 pKa = 4.33LAEE72 pKa = 4.33EE73 pKa = 5.12DD74 pKa = 3.85NNQPTTLTPQQLTLEE89 pKa = 4.23VRR91 pKa = 11.84RR92 pKa = 11.84RR93 pKa = 11.84LSTLL97 pKa = 3.08

Molecular weight: 11.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O55377|O55377_9GEMI Replication enhancer OS=Cowpea golden mosaic virus-[Nigeria] OX=223260 GN=c3 PE=3 SV=1
MM1 pKa = 7.3KK2 pKa = 10.29RR3 pKa = 11.84SADD6 pKa = 3.9TIYY9 pKa = 9.88STPSKK14 pKa = 10.4ARR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84LTFDD22 pKa = 3.17TPRR25 pKa = 11.84ATPASLGRR33 pKa = 11.84SNKK36 pKa = 8.59RR37 pKa = 11.84RR38 pKa = 11.84AWEE41 pKa = 3.71NRR43 pKa = 11.84PMNRR47 pKa = 11.84KK48 pKa = 9.3PMMYY52 pKa = 9.95RR53 pKa = 11.84LWRR56 pKa = 11.84SPDD59 pKa = 3.19VPYY62 pKa = 10.96GCEE65 pKa = 4.42GPCKK69 pKa = 9.85VQSFEE74 pKa = 3.87ARR76 pKa = 11.84HH77 pKa = 5.6DD78 pKa = 3.99VKK80 pKa = 10.69HH81 pKa = 6.21TGTFQCCSDD90 pKa = 3.55VTRR93 pKa = 11.84GMGLTHH99 pKa = 7.12RR100 pKa = 11.84VGKK103 pKa = 10.38RR104 pKa = 11.84FTIKK108 pKa = 10.0TIGIWGKK115 pKa = 9.62IWMDD119 pKa = 4.38DD120 pKa = 3.66NIKK123 pKa = 10.63LKK125 pKa = 10.47NHH127 pKa = 4.99TNIVIFFLVRR137 pKa = 11.84DD138 pKa = 4.06RR139 pKa = 11.84RR140 pKa = 11.84PSGEE144 pKa = 3.85PVSFGSLFNMFDD156 pKa = 3.84NEE158 pKa = 4.16PTTATVKK165 pKa = 10.33QEE167 pKa = 3.64YY168 pKa = 9.64RR169 pKa = 11.84DD170 pKa = 3.43RR171 pKa = 11.84FQVMRR176 pKa = 11.84RR177 pKa = 11.84FHH179 pKa = 6.81ASVTGGQYY187 pKa = 10.88ASKK190 pKa = 10.1EE191 pKa = 3.79QALVKK196 pKa = 10.53RR197 pKa = 11.84FFRR200 pKa = 11.84NINHH204 pKa = 5.78RR205 pKa = 11.84VVYY208 pKa = 9.37NQQEE212 pKa = 3.95GAEE215 pKa = 4.27YY216 pKa = 10.6KK217 pKa = 10.21NHH219 pKa = 6.87HH220 pKa = 6.68EE221 pKa = 4.18NALMLYY227 pKa = 7.32MACSHH232 pKa = 6.95ASNPVYY238 pKa = 10.18ATIKK242 pKa = 9.42VRR244 pKa = 11.84IYY246 pKa = 10.55FYY248 pKa = 11.34DD249 pKa = 4.0SITNN253 pKa = 3.6

Molecular weight: 29.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1083

97

352

180.5

20.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.817 ± 0.599

2.308 ± 0.449

5.078 ± 0.382

4.801 ± 0.572

4.432 ± 0.446

4.894 ± 0.405

3.693 ± 0.433

5.91 ± 0.838

5.54 ± 0.932

7.849 ± 1.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.585 ± 0.6

5.725 ± 0.746

5.633 ± 0.597

4.34 ± 0.419

7.202 ± 1.327

8.31 ± 1.215

5.725 ± 1.162

5.355 ± 0.57

1.385 ± 0.292

3.416 ± 0.252
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski