Furculomyces boomerangus

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Zoopagomycota; Kickxellomycotina; Harpellomycetes; Harpellales; Harpellaceae; Furculomyces

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6645 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T9Z5P6|A0A2T9Z5P6_9FUNG 2Fe-2S ferredoxin-type domain-containing protein OS=Furculomyces boomerangus OX=61424 GN=BB559_000328 PE=4 SV=1
MM1 pKa = 7.12QCPFSSYY8 pKa = 10.33CKK10 pKa = 10.31KK11 pKa = 10.21VDD13 pKa = 3.51NMLISCIPIIPKK25 pKa = 8.05TTVSVPSTTTFLPTLTSDD43 pKa = 3.22SSLATTSLAAEE54 pKa = 4.39TGSGQEE60 pKa = 3.82PTQIDD65 pKa = 3.8VNATSTGALSTDD77 pKa = 3.03SDD79 pKa = 3.76VTIIVTEE86 pKa = 4.09PPPTITEE93 pKa = 4.02TDD95 pKa = 3.34QSVVTLFTT103 pKa = 4.03

Molecular weight: 10.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T9YBV1|A0A2T9YBV1_9FUNG Uncharacterized protein OS=Furculomyces boomerangus OX=61424 GN=BB559_002007 PE=3 SV=1
MM1 pKa = 7.71RR2 pKa = 11.84SSSSSLSLSQSLNSSSSQLRR22 pKa = 11.84SSSSSQLRR30 pKa = 11.84SSSSSILSSQPTMSSNSPSFSQSVRR55 pKa = 11.84SSSIPASSQPPRR67 pKa = 11.84PSNSPTSSQPPRR79 pKa = 11.84PSNSPTFSQPPRR91 pKa = 11.84PSNSPTFSQPPRR103 pKa = 11.84PSNSPTSNQPPRR115 pKa = 11.84PSTTPASSQPPRR127 pKa = 11.84PSTTPTSSQPPRR139 pKa = 11.84PSNSPTSNQPPRR151 pKa = 11.84PSTTPASSQPPRR163 pKa = 11.84PSTTPASSQPLRR175 pKa = 11.84PSNNPTSNQPPRR187 pKa = 11.84PSNNPTSSQPSRR199 pKa = 11.84PSNNPTSSQPARR211 pKa = 11.84PSSSSPPRR219 pKa = 11.84QSTRR223 pKa = 11.84PPSNSPPSQPARR235 pKa = 11.84PSSSSPPRR243 pKa = 11.84QSSRR247 pKa = 11.84PPSNSPPRR255 pKa = 11.84QSSRR259 pKa = 11.84PPSNSPPRR267 pKa = 11.84QSSRR271 pKa = 11.84PPSNSPPRR279 pKa = 11.84QSSRR283 pKa = 11.84PPSNSPPRR291 pKa = 11.84QSSRR295 pKa = 11.84PPSNSSRR302 pKa = 11.84RR303 pKa = 3.68

Molecular weight: 31.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6645

0

6645

3304190

50

7614

497.2

56.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.723 ± 0.026

1.174 ± 0.012

5.362 ± 0.02

6.513 ± 0.028

4.366 ± 0.019

5.235 ± 0.027

1.898 ± 0.012

7.18 ± 0.025

7.974 ± 0.033

8.802 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.075 ± 0.01

7.291 ± 0.034

4.272 ± 0.024

3.797 ± 0.018

3.885 ± 0.018

9.739 ± 0.043

5.847 ± 0.024

5.475 ± 0.023

0.865 ± 0.008

3.526 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski