Chromatiaceae bacterium No.7

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Chromatiaceae; Caldichromatium

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2609 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G7VFI7|A0A6G7VFI7_9GAMM Glycosyltransferase OS=Chromatiaceae bacterium No.7 OX=2699430 GN=GWK36_13615 PE=4 SV=1
MM1 pKa = 7.49SLALTDD7 pKa = 4.29ADD9 pKa = 4.63LSLTEE14 pKa = 4.06SARR17 pKa = 11.84LKK19 pKa = 9.78ITDD22 pKa = 4.61LLHH25 pKa = 6.72EE26 pKa = 4.9VDD28 pKa = 6.13DD29 pKa = 5.04SIQGVRR35 pKa = 11.84VYY37 pKa = 9.61ATAGGCSGISFGMTFTDD54 pKa = 4.56VIYY57 pKa = 10.85EE58 pKa = 3.99EE59 pKa = 5.48DD60 pKa = 3.75GVLSLEE66 pKa = 4.28GCKK69 pKa = 10.36VVVDD73 pKa = 4.77DD74 pKa = 4.81GTLEE78 pKa = 3.92YY79 pKa = 10.9LRR81 pKa = 11.84GVEE84 pKa = 4.17IDD86 pKa = 4.23FIDD89 pKa = 4.74RR90 pKa = 11.84GDD92 pKa = 3.75GQATFIFNNLPQIGGGCGSGCGSSGGGCCC121 pKa = 5.27

Molecular weight: 12.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G7VG99|A0A6G7VG99_9GAMM Uncharacterized protein OS=Chromatiaceae bacterium No.7 OX=2699430 GN=GWK36_14560 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.4RR3 pKa = 11.84TYY5 pKa = 10.22QPSRR9 pKa = 11.84LKK11 pKa = 10.4RR12 pKa = 11.84VRR14 pKa = 11.84THH16 pKa = 5.85GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.88VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.3GRR39 pKa = 11.84ARR41 pKa = 11.84LIPP44 pKa = 4.0

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2609

0

2609

829857

17

2643

318.1

35.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.355 ± 0.056

1.119 ± 0.019

5.192 ± 0.033

6.354 ± 0.05

3.302 ± 0.031

7.871 ± 0.05

2.312 ± 0.026

5.517 ± 0.038

2.71 ± 0.033

12.131 ± 0.082

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.002 ± 0.022

2.363 ± 0.03

5.7 ± 0.044

4.164 ± 0.039

8.0 ± 0.05

4.861 ± 0.037

4.605 ± 0.042

6.469 ± 0.037

1.516 ± 0.023

2.458 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski