HIV-1 CRF04_cpx

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus; Human immunodeficiency virus 1; HIV-1 recombinants; HIV-1 circulating recombinant forms

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9YX51|Q9YX51_9HIV1 Protein Rev OS=HIV-1 CRF04_cpx OX=540992 GN=rev PE=3 SV=1
MM1 pKa = 7.46LFWEE5 pKa = 4.29IWAIVGLVVALIIVIVVWTLVFIEE29 pKa = 4.19YY30 pKa = 10.27KK31 pKa = 9.83KK32 pKa = 10.62LRR34 pKa = 11.84RR35 pKa = 11.84QRR37 pKa = 11.84RR38 pKa = 11.84IDD40 pKa = 3.24SLYY43 pKa = 9.46NRR45 pKa = 11.84IRR47 pKa = 11.84EE48 pKa = 4.05RR49 pKa = 11.84AEE51 pKa = 4.01DD52 pKa = 3.71SGNEE56 pKa = 3.89SDD58 pKa = 5.6GDD60 pKa = 3.98AEE62 pKa = 4.2EE63 pKa = 5.39LSTLVGMGNFDD74 pKa = 3.42PWVGDD79 pKa = 3.57NLL81 pKa = 4.05

Molecular weight: 9.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9YX52|Q9YX52_9HIV1 Protein Tat OS=HIV-1 CRF04_cpx OX=540992 GN=tat PE=3 SV=1
MM1 pKa = 7.85AGRR4 pKa = 11.84SGNIDD9 pKa = 3.13EE10 pKa = 5.75DD11 pKa = 3.87LFKK14 pKa = 10.69AARR17 pKa = 11.84AIKK20 pKa = 9.89ILYY23 pKa = 8.99QSNPYY28 pKa = 9.2PNNPTGTRR36 pKa = 11.84QARR39 pKa = 11.84RR40 pKa = 11.84NRR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84WRR47 pKa = 11.84ARR49 pKa = 11.84QKK51 pKa = 10.39QIHH54 pKa = 6.0SLSEE58 pKa = 4.53RR59 pKa = 11.84ILATYY64 pKa = 10.0LGRR67 pKa = 11.84PAEE70 pKa = 4.23PVPLQLPPLEE80 pKa = 5.12KK81 pKa = 10.13LTLNCSEE88 pKa = 4.73DD89 pKa = 3.81CGTSGDD95 pKa = 4.18KK96 pKa = 11.07GVGSPQVSVEE106 pKa = 4.02LPAVLGTGAKK116 pKa = 9.46EE117 pKa = 3.74

Molecular weight: 12.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

2625

81

1002

328.1

37.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.59 ± 0.524

2.171 ± 0.562

4.305 ± 0.438

7.352 ± 0.657

2.933 ± 0.219

7.39 ± 0.278

2.019 ± 0.283

6.895 ± 0.927

7.39 ± 0.776

7.429 ± 0.549

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.095 ± 0.346

4.914 ± 1.01

5.829 ± 0.406

5.448 ± 0.746

5.6 ± 0.791

4.99 ± 0.571

5.981 ± 0.782

5.676 ± 0.547

2.552 ± 0.215

2.438 ± 0.316
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski