Duck faeces associated circular DNA virus 3

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A168MFT2|A0A168MFT2_9VIRU Replication associated protein OS=Duck faeces associated circular DNA virus 3 OX=1843770 PE=3 SV=1
MM1 pKa = 7.49EE2 pKa = 4.81HH3 pKa = 6.44HH4 pKa = 5.92QQIIQTPAIYY14 pKa = 10.17EE15 pKa = 4.07FRR17 pKa = 11.84GVRR20 pKa = 11.84ALITYY25 pKa = 7.42PQCNDD30 pKa = 3.02GNKK33 pKa = 10.2EE34 pKa = 3.89EE35 pKa = 5.35LLTFLKK41 pKa = 9.95TIYY44 pKa = 10.12NNKK47 pKa = 8.12IDD49 pKa = 4.21YY50 pKa = 10.55IVVCKK55 pKa = 10.03EE56 pKa = 3.82DD57 pKa = 3.15HH58 pKa = 6.72HH59 pKa = 6.18EE60 pKa = 4.11TDD62 pKa = 3.53GEE64 pKa = 4.47HH65 pKa = 4.54YY66 pKa = 10.09HH67 pKa = 7.77AYY69 pKa = 9.84IKK71 pKa = 10.68FSTRR75 pKa = 11.84AVVNANHH82 pKa = 5.88LTYY85 pKa = 10.6RR86 pKa = 11.84GIRR89 pKa = 11.84PNLEE93 pKa = 4.19KK94 pKa = 10.96VQSTAWKK101 pKa = 9.13AVRR104 pKa = 11.84YY105 pKa = 8.81VKK107 pKa = 10.49KK108 pKa = 10.31DD109 pKa = 3.21NNYY112 pKa = 9.51IEE114 pKa = 5.57EE115 pKa = 4.55GTCTDD120 pKa = 3.48VQKK123 pKa = 10.3LTTQEE128 pKa = 3.77KK129 pKa = 9.94YY130 pKa = 10.93KK131 pKa = 10.64LIKK134 pKa = 9.58EE135 pKa = 3.94KK136 pKa = 10.16TYY138 pKa = 10.73IEE140 pKa = 4.16IFEE143 pKa = 4.41MATLSLPEE151 pKa = 4.11LCKK154 pKa = 10.7VKK156 pKa = 10.46QIQKK160 pKa = 10.19EE161 pKa = 4.2LIVNDD166 pKa = 3.35WPMNGSKK173 pKa = 10.2NRR175 pKa = 11.84EE176 pKa = 4.14VYY178 pKa = 8.89WFYY181 pKa = 11.54GPTGTGKK188 pKa = 7.74TRR190 pKa = 11.84KK191 pKa = 7.78ATEE194 pKa = 3.95MMIQLYY200 pKa = 9.04GEE202 pKa = 4.24KK203 pKa = 9.48WVSLTGNLRR212 pKa = 11.84TFFDD216 pKa = 4.61PYY218 pKa = 10.64NGEE221 pKa = 3.97KK222 pKa = 10.7GVIFDD227 pKa = 5.72DD228 pKa = 3.36IRR230 pKa = 11.84KK231 pKa = 9.96GSIEE235 pKa = 4.36WNTLLTITDD244 pKa = 4.2RR245 pKa = 11.84YY246 pKa = 7.67RR247 pKa = 11.84TSVNVKK253 pKa = 9.63GSRR256 pKa = 11.84IPWLAEE262 pKa = 3.81TIIFTSPQHH271 pKa = 6.01FNDD274 pKa = 3.09VFTTEE279 pKa = 4.1KK280 pKa = 10.72DD281 pKa = 3.32GEE283 pKa = 4.12RR284 pKa = 11.84AQWDD288 pKa = 4.27GIEE291 pKa = 4.0QFEE294 pKa = 4.42RR295 pKa = 11.84RR296 pKa = 11.84IKK298 pKa = 9.81EE299 pKa = 3.91VKK301 pKa = 9.94EE302 pKa = 4.08FNN304 pKa = 3.56

Molecular weight: 35.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A168MFT2|A0A168MFT2_9VIRU Replication associated protein OS=Duck faeces associated circular DNA virus 3 OX=1843770 PE=3 SV=1
MM1 pKa = 7.25ARR3 pKa = 11.84YY4 pKa = 8.33YY5 pKa = 10.67KK6 pKa = 10.24YY7 pKa = 10.09RR8 pKa = 11.84RR9 pKa = 11.84VYY11 pKa = 10.33KK12 pKa = 10.16KK13 pKa = 10.18VYY15 pKa = 8.72PRR17 pKa = 11.84KK18 pKa = 8.66RR19 pKa = 11.84WASNIVTKK27 pKa = 10.85NVLLTVPANEE37 pKa = 3.82KK38 pKa = 9.4TVFSFSTLVANSAQTVTPTPTLLKK62 pKa = 10.48FGRR65 pKa = 11.84CKK67 pKa = 10.29IKK69 pKa = 10.73GDD71 pKa = 3.05IRR73 pKa = 11.84TDD75 pKa = 3.21VASEE79 pKa = 3.81NNYY82 pKa = 9.92VSGIMYY88 pKa = 9.34VIYY91 pKa = 10.46VPEE94 pKa = 4.53GFAVSPTLISQHH106 pKa = 6.24PEE108 pKa = 3.85YY109 pKa = 10.51IIGWTQISFDD119 pKa = 3.88SGNTFSFSSSLKK131 pKa = 10.23RR132 pKa = 11.84NLNSGDD138 pKa = 3.93RR139 pKa = 11.84IDD141 pKa = 5.5LFFSVDD147 pKa = 3.51SVNSVSAVRR156 pKa = 11.84NFNMYY161 pKa = 8.39FTAQYY166 pKa = 6.57WTSSAA171 pKa = 3.62

Molecular weight: 19.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

475

171

304

237.5

27.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.421 ± 0.799

1.053 ± 0.262

4.211 ± 0.393

6.737 ± 2.462

5.053 ± 0.773

5.053 ± 0.537

1.895 ± 0.733

7.368 ± 0.851

7.789 ± 1.087

5.684 ± 0.236

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.684 ± 0.039

6.316 ± 0.393

3.368 ± 0.406

3.579 ± 0.694

5.474 ± 0.21

6.316 ± 3.339

8.842 ± 0.367

7.368 ± 1.44

2.105 ± 0.196

5.684 ± 0.419
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski