Caulifigura coniformis
Average proteome isoelectric point is 6.59
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5497 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A517SIB0|A0A517SIB0_9PLAN N-acetylglucosamine-6-phosphate deacetylase OS=Caulifigura coniformis OX=2527983 GN=nagA_6 PE=3 SV=1
MM1 pKa = 7.47 LFSRR5 pKa = 11.84 YY6 pKa = 7.52 ATCIRR11 pKa = 11.84 CCALLIITAVGLPARR26 pKa = 11.84 AEE28 pKa = 4.14 IITFDD33 pKa = 4.76 DD34 pKa = 4.42 LPPGQGTQYY43 pKa = 10.95 SGADD47 pKa = 3.43 VVGSYY52 pKa = 11.17 YY53 pKa = 11.06 YY54 pKa = 10.97 GDD56 pKa = 3.99 SPDD59 pKa = 3.53 GVIVEE64 pKa = 4.46 GTYY67 pKa = 10.85 GPVYY71 pKa = 10.4 EE72 pKa = 4.61 STFTSGGAGFINRR85 pKa = 11.84 VDD87 pKa = 3.37 QTYY90 pKa = 10.65 GSWSGFSYY98 pKa = 11.33 SNVSDD103 pKa = 2.97 IVTPGFEE110 pKa = 4.19 NQYY113 pKa = 11.16 AAYY116 pKa = 8.24 TLSDD120 pKa = 4.27 PGSGMGPGVDD130 pKa = 3.15 NYY132 pKa = 11.17 GIAFGYY138 pKa = 10.84 DD139 pKa = 3.44 DD140 pKa = 4.35 IVPNLFDD147 pKa = 5.69 PDD149 pKa = 4.23 AFDD152 pKa = 3.96 PTDD155 pKa = 3.37 IADD158 pKa = 4.67 LFRR161 pKa = 11.84 LPTLSLPTGMAAVGMYY177 pKa = 7.67 VTNTTYY183 pKa = 11.24 AALSMLGGDD192 pKa = 3.83 SFGKK196 pKa = 8.79 TFGGASGDD204 pKa = 3.69 DD205 pKa = 3.69 PDD207 pKa = 4.03 WFKK210 pKa = 10.91 LTAYY214 pKa = 10.45 GIGADD219 pKa = 3.85 GLAMAEE225 pKa = 4.1 SVEE228 pKa = 5.11 FYY230 pKa = 10.76 LADD233 pKa = 3.54 YY234 pKa = 10.96 RR235 pKa = 11.84 FDD237 pKa = 5.56 DD238 pKa = 3.72 NSLDD242 pKa = 4.03 YY243 pKa = 10.92 IVDD246 pKa = 3.2 DD247 pKa = 4.17 WTWMDD252 pKa = 4.46 LSALADD258 pKa = 3.62 AKK260 pKa = 10.93 SLHH263 pKa = 6.43 FNLASSDD270 pKa = 3.55 AGDD273 pKa = 3.62 FGMNTPAYY281 pKa = 10.0 FAMDD285 pKa = 4.45 DD286 pKa = 3.97 LQLEE290 pKa = 4.54 AVPEE294 pKa = 4.16 PTSLMFAAVTGLGVVLRR311 pKa = 11.84 RR312 pKa = 11.84 RR313 pKa = 11.84 GLRR316 pKa = 11.84 KK317 pKa = 9.73 KK318 pKa = 10.51 SAA320 pKa = 3.26
Molecular weight: 34.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.772
IPC2_protein 3.732
IPC_protein 3.77
Toseland 3.528
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.757
Rodwell 3.592
Grimsley 3.439
Solomon 3.783
Lehninger 3.732
Nozaki 3.897
DTASelect 4.215
Thurlkill 3.592
EMBOSS 3.757
Sillero 3.897
Patrickios 0.388
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.803
Protein with the highest isoelectric point:
>tr|A0A517SGF1|A0A517SGF1_9PLAN Uncharacterized protein OS=Caulifigura coniformis OX=2527983 GN=Pan44_32440 PE=4 SV=1
MM1 pKa = 7.64 AKK3 pKa = 9.89 NAKK6 pKa = 8.9 KK7 pKa = 10.38 LKK9 pKa = 9.73 RR10 pKa = 11.84 ANHH13 pKa = 5.73 GKK15 pKa = 10.04 RR16 pKa = 11.84 PASHH20 pKa = 7.08 KK21 pKa = 10.44 ARR23 pKa = 11.84 RR24 pKa = 11.84 AKK26 pKa = 10.3 RR27 pKa = 11.84 AMIKK31 pKa = 8.79 TT32 pKa = 3.78
Molecular weight: 3.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 10.921
IPC_protein 12.442
Toseland 12.632
ProMoST 13.1
Dawson 12.632
Bjellqvist 12.618
Wikipedia 13.1
Rodwell 12.574
Grimsley 12.676
Solomon 13.115
Lehninger 13.013
Nozaki 12.632
DTASelect 12.618
Thurlkill 12.632
EMBOSS 13.13
Sillero 12.632
Patrickios 12.31
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 8.985
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5497
0
5497
1946564
29
3238
354.1
38.78
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.725 ± 0.039
1.106 ± 0.013
5.679 ± 0.026
6.015 ± 0.028
3.703 ± 0.02
8.087 ± 0.03
2.097 ± 0.015
4.593 ± 0.023
3.683 ± 0.029
9.876 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.124 ± 0.014
2.851 ± 0.027
5.733 ± 0.03
3.615 ± 0.024
7.267 ± 0.033
6.147 ± 0.022
5.559 ± 0.031
7.41 ± 0.028
1.564 ± 0.015
2.168 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here