Amino acid dipepetide frequency for Mycolicibacterium sp. P9-64

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.268AlaAla: 19.268 ± 0.115	1.027AlaCys: 1.027 ± 0.027	8.324AlaAsp: 8.324 ± 0.076	7.525AlaGlu: 7.525 ± 0.077	3.612AlaPhe: 3.612 ± 0.046	11.356AlaGly: 11.356 ± 0.087	2.575AlaHis: 2.575 ± 0.036	5.596AlaIle: 5.596 ± 0.053	3.204AlaLys: 3.204 ± 0.042	12.873AlaLeu: 12.873 ± 0.093	3.004AlaMet: 3.004 ± 0.036	2.773AlaAsn: 2.773 ± 0.042	6.178AlaPro: 6.178 ± 0.073	3.764AlaGln: 3.764 ± 0.038	8.092AlaArg: 8.092 ± 0.063	6.513AlaSer: 6.513 ± 0.061	7.57AlaThr: 7.57 ± 0.065	11.628AlaVal: 11.628 ± 0.087	1.632AlaTrp: 1.632 ± 0.029	2.4AlaTyr: 2.4 ± 0.034	0.0AlaXaa: 0.0 ± 0.0
Cys	1.037CysAla: 1.037 ± 0.023	0.084CysCys: 0.084 ± 0.007	0.526CysAsp: 0.526 ± 0.016	0.385CysGlu: 0.385 ± 0.013	0.227CysPhe: 0.227 ± 0.011	0.938CysGly: 0.938 ± 0.022	0.185CysHis: 0.185 ± 0.01	0.279CysIle: 0.279 ± 0.011	0.135CysLys: 0.135 ± 0.008	0.63CysLeu: 0.63 ± 0.019	0.129CysMet: 0.129 ± 0.006	0.169CysAsn: 0.169 ± 0.009	0.476CysPro: 0.476 ± 0.017	0.22CysGln: 0.22 ± 0.011	0.527CysArg: 0.527 ± 0.016	0.485CysSer: 0.485 ± 0.016	0.508CysThr: 0.508 ± 0.018	0.691CysVal: 0.691 ± 0.018	0.112CysTrp: 0.112 ± 0.007	0.172CysTyr: 0.172 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	8.028AspAla: 8.028 ± 0.075	0.45AspCys: 0.45 ± 0.016	4.619AspAsp: 4.619 ± 0.066	4.119AspGlu: 4.119 ± 0.047	1.834AspPhe: 1.834 ± 0.03	6.337AspGly: 6.337 ± 0.058	1.428AspHis: 1.428 ± 0.024	2.431AspIle: 2.431 ± 0.038	1.299AspLys: 1.299 ± 0.026	6.063AspLeu: 6.063 ± 0.061	1.048AspMet: 1.048 ± 0.022	1.152AspAsn: 1.152 ± 0.027	4.251AspPro: 4.251 ± 0.044	1.833AspGln: 1.833 ± 0.027	4.448AspArg: 4.448 ± 0.052	2.843AspSer: 2.843 ± 0.043	3.195AspThr: 3.195 ± 0.049	6.06AspVal: 6.06 ± 0.058	0.997AspTrp: 0.997 ± 0.023	1.449AspTyr: 1.449 ± 0.03	0.0AspXaa: 0.0 ± 0.0
Glu	6.103GluAla: 6.103 ± 0.064	0.365GluCys: 0.365 ± 0.013	2.419GluAsp: 2.419 ± 0.03	2.278GluGlu: 2.278 ± 0.037	1.778GluPhe: 1.778 ± 0.032	3.337GluGly: 3.337 ± 0.047	1.479GluHis: 1.479 ± 0.027	2.556GluIle: 2.556 ± 0.036	1.293GluLys: 1.293 ± 0.025	6.395GluLeu: 6.395 ± 0.075	1.09GluMet: 1.09 ± 0.024	1.138GluAsn: 1.138 ± 0.025	3.03GluPro: 3.03 ± 0.043	2.054GluGln: 2.054 ± 0.036	4.341GluArg: 4.341 ± 0.051	2.789GluSer: 2.789 ± 0.043	2.665GluThr: 2.665 ± 0.036	4.265GluVal: 4.265 ± 0.05	0.746GluTrp: 0.746 ± 0.021	1.109GluTyr: 1.109 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	4.094PheAla: 4.094 ± 0.049	0.291PheCys: 0.291 ± 0.011	2.531PheAsp: 2.531 ± 0.038	1.58PheGlu: 1.58 ± 0.027	1.03PhePhe: 1.03 ± 0.025	3.579PheGly: 3.579 ± 0.047	0.663PheHis: 0.663 ± 0.019	1.187PheIle: 1.187 ± 0.025	0.62PheLys: 0.62 ± 0.016	2.565PheLeu: 2.565 ± 0.038	0.513PheMet: 0.513 ± 0.016	0.82PheAsn: 0.82 ± 0.021	1.401PhePro: 1.401 ± 0.025	0.694PheGln: 0.694 ± 0.018	1.616PheArg: 1.616 ± 0.026	1.754PheSer: 1.754 ± 0.033	2.149PheThr: 2.149 ± 0.037	2.776PheVal: 2.776 ± 0.034	0.441PheTrp: 0.441 ± 0.016	0.699PheTyr: 0.699 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	10.2GlyAla: 10.2 ± 0.074	0.8GlyCys: 0.8 ± 0.019	5.135GlyAsp: 5.135 ± 0.053	4.253GlyGlu: 4.253 ± 0.046	3.21GlyPhe: 3.21 ± 0.043	7.981GlyGly: 7.981 ± 0.12	2.094GlyHis: 2.094 ± 0.031	4.421GlyIle: 4.421 ± 0.048	2.349GlyLys: 2.349 ± 0.034	8.664GlyLeu: 8.664 ± 0.072	2.154GlyMet: 2.154 ± 0.031	2.128GlyAsn: 2.128 ± 0.039	4.494GlyPro: 4.494 ± 0.053	2.738GlyGln: 2.738 ± 0.034	6.017GlyArg: 6.017 ± 0.062	5.348GlySer: 5.348 ± 0.054	5.535GlyThr: 5.535 ± 0.079	7.842GlyVal: 7.842 ± 0.061	1.629GlyTrp: 1.629 ± 0.032	2.433GlyTyr: 2.433 ± 0.038	0.0GlyXaa: 0.0 ± 0.0
His	2.463HisAla: 2.463 ± 0.039	0.201HisCys: 0.201 ± 0.008	1.49HisAsp: 1.49 ± 0.029	1.076HisGlu: 1.076 ± 0.023	0.707HisPhe: 0.707 ± 0.018	2.169HisGly: 2.169 ± 0.031	0.696HisHis: 0.696 ± 0.02	0.757HisIle: 0.757 ± 0.019	0.301HisLys: 0.301 ± 0.013	2.162HisLeu: 2.162 ± 0.035	0.371HisMet: 0.371 ± 0.011	0.451HisAsn: 0.451 ± 0.015	1.631HisPro: 1.631 ± 0.03	0.719HisGln: 0.719 ± 0.018	1.747HisArg: 1.747 ± 0.029	1.107HisSer: 1.107 ± 0.024	1.189HisThr: 1.189 ± 0.022	1.897HisVal: 1.897 ± 0.025	0.382HisTrp: 0.382 ± 0.013	0.54HisTyr: 0.54 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	6.604IleAla: 6.604 ± 0.057	0.391IleCys: 0.391 ± 0.013	3.526IleAsp: 3.526 ± 0.04	2.631IleGlu: 2.631 ± 0.042	1.059IlePhe: 1.059 ± 0.024	4.812IleGly: 4.812 ± 0.049	0.789IleHis: 0.789 ± 0.018	1.506IleIle: 1.506 ± 0.031	0.957IleLys: 0.957 ± 0.025	3.169IleLeu: 3.169 ± 0.044	0.598IleMet: 0.598 ± 0.018	1.152IleAsn: 1.152 ± 0.023	2.362IlePro: 2.362 ± 0.03	0.987IleGln: 0.987 ± 0.019	2.607IleArg: 2.607 ± 0.034	2.41IleSer: 2.41 ± 0.036	2.924IleThr: 2.924 ± 0.048	4.077IleVal: 4.077 ± 0.049	0.482IleTrp: 0.482 ± 0.017	0.779IleTyr: 0.779 ± 0.022	0.0IleXaa: 0.0 ± 0.0
Lys	2.831LysAla: 2.831 ± 0.043	0.131LysCys: 0.131 ± 0.008	1.22LysAsp: 1.22 ± 0.026	0.974LysGlu: 0.974 ± 0.023	0.676LysPhe: 0.676 ± 0.018	1.641LysGly: 1.641 ± 0.033	0.507LysHis: 0.507 ± 0.016	1.031LysIle: 1.031 ± 0.023	0.692LysLys: 0.692 ± 0.022	2.244LysLeu: 2.244 ± 0.038	0.517LysMet: 0.517 ± 0.018	0.547LysAsn: 0.547 ± 0.014	1.525LysPro: 1.525 ± 0.029	0.772LysGln: 0.772 ± 0.02	1.589LysArg: 1.589 ± 0.028	1.364LysSer: 1.364 ± 0.026	1.48LysThr: 1.48 ± 0.026	1.973LysVal: 1.973 ± 0.031	0.329LysTrp: 0.329 ± 0.013	0.508LysTyr: 0.508 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	13.809LeuAla: 13.809 ± 0.102	0.772LeuCys: 0.772 ± 0.019	6.516LeuAsp: 6.516 ± 0.069	4.454LeuGlu: 4.454 ± 0.053	2.763LeuPhe: 2.763 ± 0.039	8.823LeuGly: 8.823 ± 0.078	2.05LeuHis: 2.05 ± 0.032	4.357LeuIle: 4.357 ± 0.05	1.955LeuLys: 1.955 ± 0.032	9.589LeuLeu: 9.589 ± 0.1	1.729LeuMet: 1.729 ± 0.026	2.176LeuAsn: 2.176 ± 0.032	5.612LeuPro: 5.612 ± 0.054	2.523LeuGln: 2.523 ± 0.037	7.134LeuArg: 7.134 ± 0.062	5.649LeuSer: 5.649 ± 0.056	6.36LeuThr: 6.36 ± 0.059	8.684LeuVal: 8.684 ± 0.07	1.162LeuTrp: 1.162 ± 0.026	1.651LeuTyr: 1.651 ± 0.026	0.0LeuXaa: 0.0 ± 0.0
Met	2.702MetAla: 2.702 ± 0.037	0.182MetCys: 0.182 ± 0.009	0.892MetAsp: 0.892 ± 0.024	0.702MetGlu: 0.702 ± 0.018	0.637MetPhe: 0.637 ± 0.018	1.559MetGly: 1.559 ± 0.027	0.433MetHis: 0.433 ± 0.012	0.927MetIle: 0.927 ± 0.021	0.499MetLys: 0.499 ± 0.016	2.042MetLeu: 2.042 ± 0.032	0.396MetMet: 0.396 ± 0.014	0.527MetAsn: 0.527 ± 0.016	1.226MetPro: 1.226 ± 0.024	0.553MetGln: 0.553 ± 0.016	1.492MetArg: 1.492 ± 0.026	1.641MetSer: 1.641 ± 0.027	1.905MetThr: 1.905 ± 0.026	1.641MetVal: 1.641 ± 0.025	0.267MetTrp: 0.267 ± 0.011	0.363MetTyr: 0.363 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.828AsnAla: 2.828 ± 0.036	0.188AsnCys: 0.188 ± 0.009	1.382AsnAsp: 1.382 ± 0.034	1.038AsnGlu: 1.038 ± 0.02	0.69AsnPhe: 0.69 ± 0.017	2.284AsnGly: 2.284 ± 0.041	0.501AsnHis: 0.501 ± 0.017	0.883AsnIle: 0.883 ± 0.021	0.479AsnLys: 0.479 ± 0.017	2.162AsnLeu: 2.162 ± 0.036	0.44AsnMet: 0.44 ± 0.014	0.585AsnAsn: 0.585 ± 0.019	1.789AsnPro: 1.789 ± 0.033	0.678AsnGln: 0.678 ± 0.017	1.496AsnArg: 1.496 ± 0.031	1.184AsnSer: 1.184 ± 0.025	1.358AsnThr: 1.358 ± 0.024	2.004AsnVal: 2.004 ± 0.031	0.384AsnTrp: 0.384 ± 0.015	0.593AsnTyr: 0.593 ± 0.019	0.0AsnXaa: 0.0 ± 0.0
Pro	6.86ProAla: 6.86 ± 0.073	0.306ProCys: 0.306 ± 0.011	4.289ProAsp: 4.289 ± 0.051	3.448ProGlu: 3.448 ± 0.042	1.646ProPhe: 1.646 ± 0.028	5.437ProGly: 5.437 ± 0.058	1.202ProHis: 1.202 ± 0.027	2.269ProIle: 2.269 ± 0.036	1.365ProLys: 1.365 ± 0.026	4.944ProLeu: 4.944 ± 0.057	1.239ProMet: 1.239 ± 0.024	1.392ProAsn: 1.392 ± 0.024	3.655ProPro: 3.655 ± 0.068	1.702ProGln: 1.702 ± 0.031	3.202ProArg: 3.202 ± 0.039	3.325ProSer: 3.325 ± 0.043	3.672ProThr: 3.672 ± 0.05	4.899ProVal: 4.899 ± 0.067	0.861ProTrp: 0.861 ± 0.019	1.163ProTyr: 1.163 ± 0.023	0.0ProXaa: 0.0 ± 0.0
Gln	3.46GlnAla: 3.46 ± 0.041	0.235GlnCys: 0.235 ± 0.011	1.297GlnAsp: 1.297 ± 0.023	1.098GlnGlu: 1.098 ± 0.024	1.009GlnPhe: 1.009 ± 0.021	1.975GlnGly: 1.975 ± 0.034	0.72GlnHis: 0.72 ± 0.019	1.512GlnIle: 1.512 ± 0.027	0.657GlnLys: 0.657 ± 0.019	3.411GlnLeu: 3.411 ± 0.044	0.676GlnMet: 0.676 ± 0.021	0.638GlnAsn: 0.638 ± 0.018	1.815GlnPro: 1.815 ± 0.029	1.161GlnGln: 1.161 ± 0.028	2.631GlnArg: 2.631 ± 0.037	1.553GlnSer: 1.553 ± 0.027	1.635GlnThr: 1.635 ± 0.027	2.405GlnVal: 2.405 ± 0.034	0.555GlnTrp: 0.555 ± 0.016	0.691GlnTyr: 0.691 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	7.828ArgAla: 7.828 ± 0.071	0.537ArgCys: 0.537 ± 0.018	4.219ArgAsp: 4.219 ± 0.046	3.594ArgGlu: 3.594 ± 0.053	2.338ArgPhe: 2.338 ± 0.04	4.999ArgGly: 4.999 ± 0.05	1.68ArgHis: 1.68 ± 0.03	3.364ArgIle: 3.364 ± 0.039	1.536ArgLys: 1.536 ± 0.028	7.09ArgLeu: 7.09 ± 0.071	1.629ArgMet: 1.629 ± 0.027	1.637ArgAsn: 1.637 ± 0.029	3.621ArgPro: 3.621 ± 0.035	2.157ArgGln: 2.157 ± 0.032	6.063ArgArg: 6.063 ± 0.062	3.999ArgSer: 3.999 ± 0.042	4.15ArgThr: 4.15 ± 0.043	5.403ArgVal: 5.403 ± 0.053	1.245ArgTrp: 1.245 ± 0.025	1.736ArgTyr: 1.736 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	7.071SerAla: 7.071 ± 0.059	0.431SerCys: 0.431 ± 0.013	3.369SerAsp: 3.369 ± 0.041	2.638SerGlu: 2.638 ± 0.039	1.74SerPhe: 1.74 ± 0.03	5.822SerGly: 5.822 ± 0.06	1.114SerHis: 1.114 ± 0.025	2.419SerIle: 2.419 ± 0.036	1.278SerLys: 1.278 ± 0.026	4.963SerLeu: 4.963 ± 0.048	1.418SerMet: 1.418 ± 0.029	1.218SerAsn: 1.218 ± 0.027	3.196SerPro: 3.196 ± 0.039	1.563SerGln: 1.563 ± 0.029	3.691SerArg: 3.691 ± 0.039	3.484SerSer: 3.484 ± 0.051	3.674SerThr: 3.674 ± 0.046	4.738SerVal: 4.738 ± 0.061	0.929SerTrp: 0.929 ± 0.02	1.208SerTyr: 1.208 ± 0.023	0.0SerXaa: 0.0 ± 0.0
Thr	7.953ThrAla: 7.953 ± 0.077	0.441ThrCys: 0.441 ± 0.014	3.857ThrAsp: 3.857 ± 0.058	3.153ThrGlu: 3.153 ± 0.041	1.952ThrPhe: 1.952 ± 0.031	5.879ThrGly: 5.879 ± 0.064	1.22ThrHis: 1.22 ± 0.024	2.783ThrIle: 2.783 ± 0.048	1.407ThrLys: 1.407 ± 0.026	5.683ThrLeu: 5.683 ± 0.058	1.277ThrMet: 1.277 ± 0.024	1.4ThrAsn: 1.4 ± 0.025	3.952ThrPro: 3.952 ± 0.052	1.505ThrGln: 1.505 ± 0.029	3.572ThrArg: 3.572 ± 0.042	3.537ThrSer: 3.537 ± 0.049	4.286ThrThr: 4.286 ± 0.072	6.033ThrVal: 6.033 ± 0.07	0.888ThrTrp: 0.888 ± 0.023	1.381ThrTyr: 1.381 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	11.803ValAla: 11.803 ± 0.081	0.738ValCys: 0.738 ± 0.019	6.006ValAsp: 6.006 ± 0.056	4.624ValGlu: 4.624 ± 0.051	2.706ValPhe: 2.706 ± 0.037	7.323ValGly: 7.323 ± 0.067	1.892ValHis: 1.892 ± 0.031	4.24ValIle: 4.24 ± 0.047	1.814ValLys: 1.814 ± 0.029	9.096ValLeu: 9.096 ± 0.079	1.704ValMet: 1.704 ± 0.029	2.146ValAsn: 2.146 ± 0.041	4.642ValPro: 4.642 ± 0.048	2.172ValGln: 2.172 ± 0.031	5.63ValArg: 5.63 ± 0.064	4.875ValSer: 4.875 ± 0.051	5.766ValThr: 5.766 ± 0.071	8.883ValVal: 8.883 ± 0.076	1.045ValTrp: 1.045 ± 0.024	1.658ValTyr: 1.658 ± 0.029	0.0ValXaa: 0.0 ± 0.0
Trp	1.548TrpAla: 1.548 ± 0.03	0.138TrpCys: 0.138 ± 0.008	0.813TrpAsp: 0.813 ± 0.021	0.601TrpGlu: 0.601 ± 0.016	0.551TrpPhe: 0.551 ± 0.016	1.052TrpGly: 1.052 ± 0.023	0.368TrpHis: 0.368 ± 0.013	0.693TrpIle: 0.693 ± 0.018	0.325TrpLys: 0.325 ± 0.012	1.695TrpLeu: 1.695 ± 0.035	0.346TrpMet: 0.346 ± 0.012	0.424TrpAsn: 0.424 ± 0.014	0.822TrpPro: 0.822 ± 0.02	0.606TrpGln: 0.606 ± 0.016	1.168TrpArg: 1.168 ± 0.025	0.964TrpSer: 0.964 ± 0.019	0.889TrpThr: 0.889 ± 0.019	1.082TrpVal: 1.082 ± 0.023	0.335TrpTrp: 0.335 ± 0.015	0.349TrpTyr: 0.349 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.424TyrAla: 2.424 ± 0.037	0.21TyrCys: 0.21 ± 0.011	1.455TyrAsp: 1.455 ± 0.03	1.059TyrGlu: 1.059 ± 0.023	0.837TyrPhe: 0.837 ± 0.019	2.125TyrGly: 2.125 ± 0.032	0.46TyrHis: 0.46 ± 0.013	0.614TyrIle: 0.614 ± 0.018	0.387TyrLys: 0.387 ± 0.014	2.327TyrLeu: 2.327 ± 0.034	0.306TyrMet: 0.306 ± 0.013	0.471TyrAsn: 0.471 ± 0.018	1.201TyrPro: 1.201 ± 0.027	0.732TyrGln: 0.732 ± 0.02	1.762TyrArg: 1.762 ± 0.03	1.138TyrSer: 1.138 ± 0.025	1.249TyrThr: 1.249 ± 0.028	1.784TyrVal: 1.784 ± 0.029	0.343TyrTrp: 0.343 ± 0.013	0.527TyrTyr: 0.527 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6799 proteins (2191175 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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