Amino acid dipepetide frequency for Burkholderia phage KS9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.456AlaAla: 16.456 ± 3.151
1.033AlaCys: 1.033 ± 0.358
8.824AlaAsp: 8.824 ± 1.139
8.665AlaGlu: 8.665 ± 1.07
3.498AlaPhe: 3.498 ± 0.467
10.335AlaGly: 10.335 ± 0.909
2.862AlaHis: 2.862 ± 0.573
6.519AlaIle: 6.519 ± 0.691
4.452AlaLys: 4.452 ± 0.665
10.732AlaLeu: 10.732 ± 1.209
3.577AlaMet: 3.577 ± 0.513
3.1AlaAsn: 3.1 ± 0.651
4.69AlaPro: 4.69 ± 0.691
5.088AlaGln: 5.088 ± 0.993
10.017AlaArg: 10.017 ± 1.13
7.473AlaSer: 7.473 ± 0.947
4.849AlaThr: 4.849 ± 0.677
9.142AlaVal: 9.142 ± 0.837
1.987AlaTrp: 1.987 ± 0.397
2.623AlaTyr: 2.623 ± 0.438
0.0AlaXaa: 0.0 ± 0.0
Cys
0.556CysAla: 0.556 ± 0.183
0.159CysCys: 0.159 ± 0.127
0.238CysAsp: 0.238 ± 0.118
0.636CysGlu: 0.636 ± 0.302
0.397CysPhe: 0.397 ± 0.198
1.113CysGly: 1.113 ± 0.307
0.318CysHis: 0.318 ± 0.164
0.238CysIle: 0.238 ± 0.126
0.477CysLys: 0.477 ± 0.173
0.556CysLeu: 0.556 ± 0.287
0.159CysMet: 0.159 ± 0.145
0.238CysAsn: 0.238 ± 0.129
0.477CysPro: 0.477 ± 0.207
0.159CysGln: 0.159 ± 0.114
1.113CysArg: 1.113 ± 0.367
0.636CysSer: 0.636 ± 0.247
0.636CysThr: 0.636 ± 0.201
0.715CysVal: 0.715 ± 0.235
0.159CysTrp: 0.159 ± 0.114
0.238CysTyr: 0.238 ± 0.149
0.0CysXaa: 0.0 ± 0.0
Asp
8.745AspAla: 8.745 ± 0.805
0.477AspCys: 0.477 ± 0.23
4.69AspAsp: 4.69 ± 0.695
4.054AspGlu: 4.054 ± 0.717
1.669AspPhe: 1.669 ± 0.398
6.996AspGly: 6.996 ± 1.052
1.033AspHis: 1.033 ± 0.302
2.305AspIle: 2.305 ± 0.461
1.669AspLys: 1.669 ± 0.358
5.088AspLeu: 5.088 ± 0.474
1.51AspMet: 1.51 ± 0.342
1.669AspAsn: 1.669 ± 0.304
2.703AspPro: 2.703 ± 0.636
2.305AspGln: 2.305 ± 0.339
4.531AspArg: 4.531 ± 0.508
3.339AspSer: 3.339 ± 0.53
2.305AspThr: 2.305 ± 0.365
4.69AspVal: 4.69 ± 0.68
1.272AspTrp: 1.272 ± 0.269
1.749AspTyr: 1.749 ± 0.428
0.0AspXaa: 0.0 ± 0.0
Glu
5.803GluAla: 5.803 ± 0.905
0.874GluCys: 0.874 ± 0.291
2.544GluAsp: 2.544 ± 0.542
2.464GluGlu: 2.464 ± 0.422
1.908GluPhe: 1.908 ± 0.412
2.623GluGly: 2.623 ± 0.621
1.431GluHis: 1.431 ± 0.387
3.1GluIle: 3.1 ± 0.358
3.418GluLys: 3.418 ± 0.617
6.042GluLeu: 6.042 ± 0.809
1.431GluMet: 1.431 ± 0.358
1.828GluAsn: 1.828 ± 0.356
2.862GluPro: 2.862 ± 0.54
2.623GluGln: 2.623 ± 0.546
4.849GluArg: 4.849 ± 0.733
4.213GluSer: 4.213 ± 0.486
2.703GluThr: 2.703 ± 0.587
3.418GluVal: 3.418 ± 0.391
1.828GluTrp: 1.828 ± 0.345
1.987GluTyr: 1.987 ± 0.374
0.0GluXaa: 0.0 ± 0.0
Phe
3.1PheAla: 3.1 ± 0.551
0.238PheCys: 0.238 ± 0.183
2.544PheAsp: 2.544 ± 0.443
2.305PheGlu: 2.305 ± 0.376
0.556PhePhe: 0.556 ± 0.208
3.736PheGly: 3.736 ± 0.465
0.397PheHis: 0.397 ± 0.143
1.113PheIle: 1.113 ± 0.391
1.351PheLys: 1.351 ± 0.332
1.59PheLeu: 1.59 ± 0.406
0.715PheMet: 0.715 ± 0.258
1.192PheAsn: 1.192 ± 0.413
1.51PhePro: 1.51 ± 0.315
1.113PheGln: 1.113 ± 0.339
1.828PheArg: 1.828 ± 0.321
1.59PheSer: 1.59 ± 0.425
1.272PheThr: 1.272 ± 0.229
2.464PheVal: 2.464 ± 0.428
0.715PheTrp: 0.715 ± 0.23
0.715PheTyr: 0.715 ± 0.256
0.0PheXaa: 0.0 ± 0.0
Gly
9.063GlyAla: 9.063 ± 0.793
0.477GlyCys: 0.477 ± 0.16
5.803GlyAsp: 5.803 ± 0.677
3.577GlyGlu: 3.577 ± 0.56
2.464GlyPhe: 2.464 ± 0.443
8.904GlyGly: 8.904 ± 0.89
0.954GlyHis: 0.954 ± 0.264
4.293GlyIle: 4.293 ± 0.624
3.339GlyLys: 3.339 ± 0.55
6.121GlyLeu: 6.121 ± 0.57
2.146GlyMet: 2.146 ± 0.524
2.226GlyAsn: 2.226 ± 0.516
2.703GlyPro: 2.703 ± 0.489
2.544GlyGln: 2.544 ± 0.392
6.519GlyArg: 6.519 ± 0.832
4.452GlySer: 4.452 ± 0.64
5.167GlyThr: 5.167 ± 0.857
6.678GlyVal: 6.678 ± 0.709
1.987GlyTrp: 1.987 ± 0.433
2.464GlyTyr: 2.464 ± 0.46
0.0GlyXaa: 0.0 ± 0.0
His
2.305HisAla: 2.305 ± 0.452
0.238HisCys: 0.238 ± 0.182
0.715HisAsp: 0.715 ± 0.218
1.51HisGlu: 1.51 ± 0.303
0.636HisPhe: 0.636 ± 0.224
1.113HisGly: 1.113 ± 0.343
0.397HisHis: 0.397 ± 0.166
0.874HisIle: 0.874 ± 0.295
0.397HisLys: 0.397 ± 0.156
1.908HisLeu: 1.908 ± 0.483
0.636HisMet: 0.636 ± 0.251
0.397HisAsn: 0.397 ± 0.168
0.636HisPro: 0.636 ± 0.284
0.397HisGln: 0.397 ± 0.179
1.59HisArg: 1.59 ± 0.437
1.51HisSer: 1.51 ± 0.419
0.556HisThr: 0.556 ± 0.217
1.192HisVal: 1.192 ± 0.263
0.159HisTrp: 0.159 ± 0.116
0.795HisTyr: 0.795 ± 0.285
0.0HisXaa: 0.0 ± 0.0
Ile
6.916IleAla: 6.916 ± 0.702
0.477IleCys: 0.477 ± 0.193
4.69IleAsp: 4.69 ± 0.559
4.372IleGlu: 4.372 ± 0.69
1.033IlePhe: 1.033 ± 0.309
4.134IleGly: 4.134 ± 0.526
0.795IleHis: 0.795 ± 0.366
1.987IleIle: 1.987 ± 0.449
1.908IleLys: 1.908 ± 0.426
2.544IleLeu: 2.544 ± 0.509
0.556IleMet: 0.556 ± 0.234
1.669IleAsn: 1.669 ± 0.32
1.59IlePro: 1.59 ± 0.343
1.51IleGln: 1.51 ± 0.315
2.862IleArg: 2.862 ± 0.402
2.623IleSer: 2.623 ± 0.425
2.782IleThr: 2.782 ± 0.486
5.167IleVal: 5.167 ± 0.521
0.556IleTrp: 0.556 ± 0.253
1.272IleTyr: 1.272 ± 0.357
0.0IleXaa: 0.0 ± 0.0
Lys
4.372LysAla: 4.372 ± 0.491
0.238LysCys: 0.238 ± 0.119
1.749LysAsp: 1.749 ± 0.377
1.749LysGlu: 1.749 ± 0.355
1.192LysPhe: 1.192 ± 0.293
2.305LysGly: 2.305 ± 0.452
0.477LysHis: 0.477 ± 0.227
1.113LysIle: 1.113 ± 0.281
2.067LysLys: 2.067 ± 0.331
3.339LysLeu: 3.339 ± 0.782
1.113LysMet: 1.113 ± 0.306
1.51LysAsn: 1.51 ± 0.348
2.146LysPro: 2.146 ± 0.397
1.749LysGln: 1.749 ± 0.345
2.623LysArg: 2.623 ± 0.431
3.259LysSer: 3.259 ± 0.526
3.021LysThr: 3.021 ± 0.528
2.782LysVal: 2.782 ± 0.496
0.477LysTrp: 0.477 ± 0.155
1.351LysTyr: 1.351 ± 0.371
0.0LysXaa: 0.0 ± 0.0
Leu
10.732LeuAla: 10.732 ± 1.317
0.318LeuCys: 0.318 ± 0.186
5.406LeuAsp: 5.406 ± 0.7
4.372LeuGlu: 4.372 ± 0.64
2.146LeuPhe: 2.146 ± 0.403
5.724LeuGly: 5.724 ± 0.753
1.351LeuHis: 1.351 ± 0.361
4.293LeuIle: 4.293 ± 0.471
2.146LeuLys: 2.146 ± 0.489
5.406LeuLeu: 5.406 ± 0.809
1.51LeuMet: 1.51 ± 0.356
2.544LeuAsn: 2.544 ± 0.383
3.339LeuPro: 3.339 ± 0.539
2.941LeuGln: 2.941 ± 0.366
7.95LeuArg: 7.95 ± 0.975
5.644LeuSer: 5.644 ± 0.59
4.929LeuThr: 4.929 ± 0.681
5.088LeuVal: 5.088 ± 0.656
0.874LeuTrp: 0.874 ± 0.271
2.146LeuTyr: 2.146 ± 0.423
0.0LeuXaa: 0.0 ± 0.0
Met
1.908MetAla: 1.908 ± 0.293
0.318MetCys: 0.318 ± 0.162
1.113MetAsp: 1.113 ± 0.254
0.556MetGlu: 0.556 ± 0.213
0.795MetPhe: 0.795 ± 0.229
1.908MetGly: 1.908 ± 0.462
0.636MetHis: 0.636 ± 0.228
1.192MetIle: 1.192 ± 0.262
1.192MetLys: 1.192 ± 0.259
2.146MetLeu: 2.146 ± 0.437
0.636MetMet: 0.636 ± 0.235
0.397MetAsn: 0.397 ± 0.138
1.908MetPro: 1.908 ± 0.35
1.033MetGln: 1.033 ± 0.234
2.226MetArg: 2.226 ± 0.456
1.828MetSer: 1.828 ± 0.393
1.431MetThr: 1.431 ± 0.326
1.828MetVal: 1.828 ± 0.401
0.159MetTrp: 0.159 ± 0.141
0.318MetTyr: 0.318 ± 0.231
0.0MetXaa: 0.0 ± 0.0
Asn
4.452AsnAla: 4.452 ± 0.631
0.318AsnCys: 0.318 ± 0.135
1.59AsnAsp: 1.59 ± 0.323
1.351AsnGlu: 1.351 ± 0.308
0.795AsnPhe: 0.795 ± 0.194
3.577AsnGly: 3.577 ± 0.526
0.318AsnHis: 0.318 ± 0.183
1.59AsnIle: 1.59 ± 0.352
1.113AsnLys: 1.113 ± 0.323
3.1AsnLeu: 3.1 ± 0.553
0.556AsnMet: 0.556 ± 0.184
0.715AsnAsn: 0.715 ± 0.271
1.908AsnPro: 1.908 ± 0.367
0.874AsnGln: 0.874 ± 0.218
2.067AsnArg: 2.067 ± 0.43
1.828AsnSer: 1.828 ± 0.296
1.59AsnThr: 1.59 ± 0.326
2.226AsnVal: 2.226 ± 0.648
0.954AsnTrp: 0.954 ± 0.263
0.874AsnTyr: 0.874 ± 0.259
0.0AsnXaa: 0.0 ± 0.0
Pro
5.644ProAla: 5.644 ± 0.752
0.238ProCys: 0.238 ± 0.165
2.703ProAsp: 2.703 ± 0.458
2.703ProGlu: 2.703 ± 0.39
0.954ProPhe: 0.954 ± 0.28
4.611ProGly: 4.611 ± 0.612
1.272ProHis: 1.272 ± 0.364
2.941ProIle: 2.941 ± 0.429
1.272ProLys: 1.272 ± 0.325
3.418ProLeu: 3.418 ± 0.409
1.113ProMet: 1.113 ± 0.306
2.941ProAsn: 2.941 ± 0.552
2.782ProPro: 2.782 ± 0.616
0.874ProGln: 0.874 ± 0.242
1.908ProArg: 1.908 ± 0.391
3.657ProSer: 3.657 ± 0.621
1.987ProThr: 1.987 ± 0.493
3.18ProVal: 3.18 ± 0.485
0.954ProTrp: 0.954 ± 0.302
0.795ProTyr: 0.795 ± 0.236
0.0ProXaa: 0.0 ± 0.0
Gln
5.008GlnAla: 5.008 ± 0.922
0.477GlnCys: 0.477 ± 0.173
1.113GlnAsp: 1.113 ± 0.304
1.908GlnGlu: 1.908 ± 0.354
0.954GlnPhe: 0.954 ± 0.376
2.544GlnGly: 2.544 ± 0.513
0.477GlnHis: 0.477 ± 0.161
2.146GlnIle: 2.146 ± 0.366
1.272GlnLys: 1.272 ± 0.306
3.021GlnLeu: 3.021 ± 0.556
0.636GlnMet: 0.636 ± 0.238
1.351GlnAsn: 1.351 ± 0.261
1.828GlnPro: 1.828 ± 0.383
1.272GlnGln: 1.272 ± 0.369
3.259GlnArg: 3.259 ± 0.514
2.385GlnSer: 2.385 ± 0.532
1.59GlnThr: 1.59 ± 0.262
3.418GlnVal: 3.418 ± 0.556
0.795GlnTrp: 0.795 ± 0.359
0.636GlnTyr: 0.636 ± 0.201
0.0GlnXaa: 0.0 ± 0.0
Arg
10.494ArgAla: 10.494 ± 1.055
0.874ArgCys: 0.874 ± 0.294
3.816ArgAsp: 3.816 ± 0.588
4.611ArgGlu: 4.611 ± 0.821
2.862ArgPhe: 2.862 ± 0.402
5.008ArgGly: 5.008 ± 0.603
1.431ArgHis: 1.431 ± 0.336
5.326ArgIle: 5.326 ± 0.704
3.259ArgLys: 3.259 ± 0.532
6.519ArgLeu: 6.519 ± 0.802
1.749ArgMet: 1.749 ± 0.401
1.828ArgAsn: 1.828 ± 0.395
3.1ArgPro: 3.1 ± 0.581
3.259ArgGln: 3.259 ± 0.508
6.201ArgArg: 6.201 ± 0.894
3.259ArgSer: 3.259 ± 0.563
3.498ArgThr: 3.498 ± 0.527
5.565ArgVal: 5.565 ± 0.775
1.033ArgTrp: 1.033 ± 0.255
2.305ArgTyr: 2.305 ± 0.55
0.0ArgXaa: 0.0 ± 0.0
Ser
10.971SerAla: 10.971 ± 1.277
0.556SerCys: 0.556 ± 0.278
4.849SerAsp: 4.849 ± 0.624
4.213SerGlu: 4.213 ± 0.59
1.908SerPhe: 1.908 ± 0.363
5.485SerGly: 5.485 ± 0.872
1.669SerHis: 1.669 ± 0.364
2.305SerIle: 2.305 ± 0.393
2.941SerLys: 2.941 ± 0.369
4.531SerLeu: 4.531 ± 0.575
1.51SerMet: 1.51 ± 0.345
2.067SerAsn: 2.067 ± 0.431
2.862SerPro: 2.862 ± 0.341
1.828SerGln: 1.828 ± 0.316
3.816SerArg: 3.816 ± 0.567
4.054SerSer: 4.054 ± 0.577
3.577SerThr: 3.577 ± 0.411
3.816SerVal: 3.816 ± 0.726
0.636SerTrp: 0.636 ± 0.239
1.59SerTyr: 1.59 ± 0.494
0.0SerXaa: 0.0 ± 0.0
Thr
6.757ThrAla: 6.757 ± 0.7
0.397ThrCys: 0.397 ± 0.156
2.941ThrAsp: 2.941 ± 0.459
1.908ThrGlu: 1.908 ± 0.386
1.669ThrPhe: 1.669 ± 0.512
4.213ThrGly: 4.213 ± 0.667
0.556ThrHis: 0.556 ± 0.206
2.464ThrIle: 2.464 ± 0.441
2.544ThrLys: 2.544 ± 0.523
3.736ThrLeu: 3.736 ± 0.545
0.636ThrMet: 0.636 ± 0.211
2.067ThrAsn: 2.067 ± 0.431
3.021ThrPro: 3.021 ± 0.466
1.987ThrGln: 1.987 ± 0.415
2.544ThrArg: 2.544 ± 0.595
3.895ThrSer: 3.895 ± 0.588
3.18ThrThr: 3.18 ± 0.562
5.247ThrVal: 5.247 ± 0.515
0.556ThrTrp: 0.556 ± 0.193
1.272ThrTyr: 1.272 ± 0.381
0.0ThrXaa: 0.0 ± 0.0
Val
9.142ValAla: 9.142 ± 0.98
0.795ValCys: 0.795 ± 0.303
4.372ValAsp: 4.372 ± 0.625
5.326ValGlu: 5.326 ± 0.494
3.1ValPhe: 3.1 ± 0.597
4.929ValGly: 4.929 ± 0.553
0.954ValHis: 0.954 ± 0.306
3.577ValIle: 3.577 ± 0.444
2.385ValLys: 2.385 ± 0.427
4.69ValLeu: 4.69 ± 0.636
2.146ValMet: 2.146 ± 0.455
2.623ValAsn: 2.623 ± 0.44
3.895ValPro: 3.895 ± 0.689
2.862ValGln: 2.862 ± 0.431
5.803ValArg: 5.803 ± 0.662
6.201ValSer: 6.201 ± 0.83
3.339ValThr: 3.339 ± 0.598
4.134ValVal: 4.134 ± 0.7
1.033ValTrp: 1.033 ± 0.348
2.544ValTyr: 2.544 ± 0.489
0.0ValXaa: 0.0 ± 0.0
Trp
1.431TrpAla: 1.431 ± 0.331
0.238TrpCys: 0.238 ± 0.156
0.636TrpAsp: 0.636 ± 0.223
0.715TrpGlu: 0.715 ± 0.239
0.397TrpPhe: 0.397 ± 0.141
0.874TrpGly: 0.874 ± 0.274
0.397TrpHis: 0.397 ± 0.219
0.874TrpIle: 0.874 ± 0.254
0.477TrpLys: 0.477 ± 0.166
1.908TrpLeu: 1.908 ± 0.326
0.715TrpMet: 0.715 ± 0.29
0.795TrpAsn: 0.795 ± 0.198
0.874TrpPro: 0.874 ± 0.308
0.636TrpGln: 0.636 ± 0.246
1.908TrpArg: 1.908 ± 0.52
1.033TrpSer: 1.033 ± 0.315
1.192TrpThr: 1.192 ± 0.265
1.033TrpVal: 1.033 ± 0.334
0.318TrpTrp: 0.318 ± 0.202
0.318TrpTyr: 0.318 ± 0.169
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.067TyrAla: 2.067 ± 0.448
0.477TyrCys: 0.477 ± 0.164
2.464TyrAsp: 2.464 ± 0.514
0.954TyrGlu: 0.954 ± 0.275
1.113TyrPhe: 1.113 ± 0.236
1.828TyrGly: 1.828 ± 0.42
0.238TyrHis: 0.238 ± 0.107
1.192TyrIle: 1.192 ± 0.349
0.954TyrLys: 0.954 ± 0.233
2.464TyrLeu: 2.464 ± 0.465
0.397TyrMet: 0.397 ± 0.177
0.636TyrAsn: 0.636 ± 0.222
1.113TyrPro: 1.113 ± 0.31
1.033TyrGln: 1.033 ± 0.253
2.385TyrArg: 2.385 ± 0.462
2.305TyrSer: 2.305 ± 0.483
1.987TyrThr: 1.987 ± 0.393
2.067TyrVal: 2.067 ± 0.418
0.318TyrTrp: 0.318 ± 0.149
0.477TyrTyr: 0.477 ± 0.19
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (12580 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski