Bacillus phage phiNIT1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Nitunavirus; Bacillus virus NIT1

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 211 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S6B6I8|S6B6I8_9CAUD Uncharacterized protein OS=Bacillus phage phiNIT1 OX=207656 GN=orf111 PE=4 SV=1
MM1 pKa = 7.2NKK3 pKa = 9.91EE4 pKa = 3.69IQFLKK9 pKa = 10.7DD10 pKa = 3.2LQQEE14 pKa = 4.74LKK16 pKa = 8.98TQDD19 pKa = 3.98NDD21 pKa = 3.43CQAAPRR27 pKa = 11.84FWTLMDD33 pKa = 3.68YY34 pKa = 10.71RR35 pKa = 11.84WVEE38 pKa = 3.91TSPDD42 pKa = 3.23EE43 pKa = 4.13YY44 pKa = 11.14EE45 pKa = 4.58RR46 pKa = 11.84ISIVFPEE53 pKa = 4.55EE54 pKa = 4.29YY55 pKa = 9.54EE56 pKa = 4.29DD57 pKa = 5.14HH58 pKa = 6.95VLDD61 pKa = 5.44DD62 pKa = 4.59LVSDD66 pKa = 4.71IINYY70 pKa = 9.61NPDD73 pKa = 2.92CSYY76 pKa = 11.18CYY78 pKa = 9.85TVDD81 pKa = 3.81HH82 pKa = 7.2DD83 pKa = 5.64AIDD86 pKa = 4.04DD87 pKa = 3.8LRR89 pKa = 11.84EE90 pKa = 3.95LVDD93 pKa = 4.18YY94 pKa = 10.88FMTDD98 pKa = 2.76EE99 pKa = 5.64DD100 pKa = 5.01EE101 pKa = 4.2LLKK104 pKa = 10.51WFQTNIDD111 pKa = 3.73SVAA114 pKa = 3.06

Molecular weight: 13.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S6BUT6|S6BUT6_9CAUD Putative capsid protein OS=Bacillus phage phiNIT1 OX=207656 GN=orf491 PE=4 SV=1
MM1 pKa = 7.38TKK3 pKa = 9.98HH4 pKa = 5.98AHH6 pKa = 5.61TSSFCWVVNCSSKK19 pKa = 11.03SKK21 pKa = 10.97ASISLTSVSILGRR34 pKa = 11.84LSPFSILRR42 pKa = 11.84IVILDD47 pKa = 3.67TPVISLRR54 pKa = 11.84RR55 pKa = 11.84CC56 pKa = 3.12

Molecular weight: 6.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

211

0

211

41846

47

1237

198.3

22.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.204 ± 0.214

0.755 ± 0.075

6.588 ± 0.158

7.977 ± 0.306

3.738 ± 0.111

6.371 ± 0.276

1.897 ± 0.122

6.067 ± 0.133

7.413 ± 0.27

8.462 ± 0.201

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.617 ± 0.089

5.226 ± 0.156

3.207 ± 0.141

3.704 ± 0.127

4.536 ± 0.138

6.5 ± 0.208

6.378 ± 0.266

6.875 ± 0.129

1.073 ± 0.083

4.411 ± 0.114
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski