Amino acid dipepetide frequency for Pectobacterium phage PM1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.305AlaAla: 9.305 ± 1.28
0.758AlaCys: 0.758 ± 0.265
4.894AlaAsp: 4.894 ± 0.642
4.411AlaGlu: 4.411 ± 0.593
3.377AlaPhe: 3.377 ± 0.475
7.513AlaGly: 7.513 ± 0.896
1.723AlaHis: 1.723 ± 0.369
5.514AlaIle: 5.514 ± 0.608
4.687AlaLys: 4.687 ± 0.795
6.962AlaLeu: 6.962 ± 0.716
2.688AlaMet: 2.688 ± 0.442
5.101AlaAsn: 5.101 ± 0.694
2.826AlaPro: 2.826 ± 0.569
3.929AlaGln: 3.929 ± 0.741
5.307AlaArg: 5.307 ± 0.9
5.859AlaSer: 5.859 ± 0.619
5.583AlaThr: 5.583 ± 0.868
6.272AlaVal: 6.272 ± 0.901
0.827AlaTrp: 0.827 ± 0.258
2.275AlaTyr: 2.275 ± 0.377
0.0AlaXaa: 0.0 ± 0.0
Cys
0.62CysAla: 0.62 ± 0.206
0.207CysCys: 0.207 ± 0.114
0.689CysAsp: 0.689 ± 0.362
1.103CysGlu: 1.103 ± 0.333
0.689CysPhe: 0.689 ± 0.212
0.758CysGly: 0.758 ± 0.266
0.414CysHis: 0.414 ± 0.2
0.551CysIle: 0.551 ± 0.188
0.689CysLys: 0.689 ± 0.251
0.896CysLeu: 0.896 ± 0.237
0.207CysMet: 0.207 ± 0.126
0.345CysAsn: 0.345 ± 0.19
0.482CysPro: 0.482 ± 0.166
0.414CysGln: 0.414 ± 0.218
0.689CysArg: 0.689 ± 0.221
0.551CysSer: 0.551 ± 0.205
1.241CysThr: 1.241 ± 0.335
0.689CysVal: 0.689 ± 0.249
0.345CysTrp: 0.345 ± 0.159
0.207CysTyr: 0.207 ± 0.123
0.0CysXaa: 0.0 ± 0.0
Asp
5.652AspAla: 5.652 ± 0.579
0.689AspCys: 0.689 ± 0.276
2.964AspAsp: 2.964 ± 0.489
3.653AspGlu: 3.653 ± 0.625
1.654AspPhe: 1.654 ± 0.325
5.79AspGly: 5.79 ± 0.606
0.689AspHis: 0.689 ± 0.223
3.584AspIle: 3.584 ± 0.398
3.309AspLys: 3.309 ± 0.467
4.618AspLeu: 4.618 ± 0.664
1.861AspMet: 1.861 ± 0.321
2.55AspAsn: 2.55 ± 0.45
2.068AspPro: 2.068 ± 0.448
0.965AspGln: 0.965 ± 0.213
2.275AspArg: 2.275 ± 0.45
4.342AspSer: 4.342 ± 0.553
3.033AspThr: 3.033 ± 0.509
4.756AspVal: 4.756 ± 0.601
1.034AspTrp: 1.034 ± 0.262
2.275AspTyr: 2.275 ± 0.342
0.0AspXaa: 0.0 ± 0.0
Glu
5.101GluAla: 5.101 ± 0.712
0.689GluCys: 0.689 ± 0.243
3.033GluAsp: 3.033 ± 0.531
5.101GluGlu: 5.101 ± 0.797
2.688GluPhe: 2.688 ± 0.38
3.584GluGly: 3.584 ± 0.492
1.861GluHis: 1.861 ± 0.351
4.549GluIle: 4.549 ± 0.822
2.826GluLys: 2.826 ± 0.413
5.859GluLeu: 5.859 ± 0.88
1.516GluMet: 1.516 ± 0.325
2.55GluAsn: 2.55 ± 0.399
1.654GluPro: 1.654 ± 0.338
2.826GluGln: 2.826 ± 0.468
3.033GluArg: 3.033 ± 0.533
2.895GluSer: 2.895 ± 0.467
3.171GluThr: 3.171 ± 0.493
4.618GluVal: 4.618 ± 0.66
0.896GluTrp: 0.896 ± 0.211
3.377GluTyr: 3.377 ± 0.41
0.0GluXaa: 0.0 ± 0.0
Phe
3.102PheAla: 3.102 ± 0.477
0.689PheCys: 0.689 ± 0.223
2.757PheAsp: 2.757 ± 0.449
2.275PheGlu: 2.275 ± 0.45
0.896PhePhe: 0.896 ± 0.261
3.033PheGly: 3.033 ± 0.401
1.103PheHis: 1.103 ± 0.266
2.206PheIle: 2.206 ± 0.418
2.275PheLys: 2.275 ± 0.407
2.55PheLeu: 2.55 ± 0.426
1.172PheMet: 1.172 ± 0.329
1.516PheAsn: 1.516 ± 0.352
1.379PhePro: 1.379 ± 0.276
1.723PheGln: 1.723 ± 0.439
2.068PheArg: 2.068 ± 0.397
2.964PheSer: 2.964 ± 0.405
1.93PheThr: 1.93 ± 0.396
2.826PheVal: 2.826 ± 0.403
0.345PheTrp: 0.345 ± 0.136
1.31PheTyr: 1.31 ± 0.289
0.0PheXaa: 0.0 ± 0.0
Gly
6.479GlyAla: 6.479 ± 1.143
0.551GlyCys: 0.551 ± 0.225
4.618GlyAsp: 4.618 ± 0.609
3.998GlyGlu: 3.998 ± 0.549
3.309GlyPhe: 3.309 ± 0.311
6.203GlyGly: 6.203 ± 0.919
2.068GlyHis: 2.068 ± 0.363
4.274GlyIle: 4.274 ± 0.502
4.756GlyLys: 4.756 ± 0.614
5.376GlyLeu: 5.376 ± 0.665
1.654GlyMet: 1.654 ± 0.465
3.86GlyAsn: 3.86 ± 0.542
1.516GlyPro: 1.516 ± 0.292
3.309GlyGln: 3.309 ± 0.52
3.653GlyArg: 3.653 ± 0.57
5.652GlySer: 5.652 ± 0.767
5.997GlyThr: 5.997 ± 0.711
6.479GlyVal: 6.479 ± 0.793
1.585GlyTrp: 1.585 ± 0.294
2.481GlyTyr: 2.481 ± 0.357
0.0GlyXaa: 0.0 ± 0.0
His
1.861HisAla: 1.861 ± 0.472
0.345HisCys: 0.345 ± 0.141
1.447HisAsp: 1.447 ± 0.299
1.172HisGlu: 1.172 ± 0.237
0.965HisPhe: 0.965 ± 0.281
1.792HisGly: 1.792 ± 0.315
0.482HisHis: 0.482 ± 0.198
0.896HisIle: 0.896 ± 0.263
1.103HisLys: 1.103 ± 0.342
1.585HisLeu: 1.585 ± 0.309
0.551HisMet: 0.551 ± 0.156
1.241HisAsn: 1.241 ± 0.326
0.827HisPro: 0.827 ± 0.237
0.689HisGln: 0.689 ± 0.204
0.551HisArg: 0.551 ± 0.23
1.172HisSer: 1.172 ± 0.304
0.896HisThr: 0.896 ± 0.261
1.723HisVal: 1.723 ± 0.276
0.414HisTrp: 0.414 ± 0.159
0.827HisTyr: 0.827 ± 0.228
0.0HisXaa: 0.0 ± 0.0
Ile
5.101IleAla: 5.101 ± 0.56
0.896IleCys: 0.896 ± 0.227
3.584IleAsp: 3.584 ± 0.584
3.309IleGlu: 3.309 ± 0.62
1.93IlePhe: 1.93 ± 0.438
3.929IleGly: 3.929 ± 0.486
0.896IleHis: 0.896 ± 0.214
2.619IleIle: 2.619 ± 0.411
4.825IleLys: 4.825 ± 0.71
4.894IleLeu: 4.894 ± 0.513
1.516IleMet: 1.516 ± 0.348
2.619IleAsn: 2.619 ± 0.401
2.688IlePro: 2.688 ± 0.446
2.688IleGln: 2.688 ± 0.373
2.55IleArg: 2.55 ± 0.432
3.791IleSer: 3.791 ± 0.496
4.274IleThr: 4.274 ± 0.612
4.136IleVal: 4.136 ± 0.689
0.758IleTrp: 0.758 ± 0.189
1.585IleTyr: 1.585 ± 0.334
0.0IleXaa: 0.0 ± 0.0
Lys
5.514LysAla: 5.514 ± 0.509
0.896LysCys: 0.896 ± 0.298
3.309LysAsp: 3.309 ± 0.699
4.411LysGlu: 4.411 ± 0.51
2.137LysPhe: 2.137 ± 0.431
2.964LysGly: 2.964 ± 0.381
1.447LysHis: 1.447 ± 0.384
3.309LysIle: 3.309 ± 0.452
2.688LysLys: 2.688 ± 0.462
5.721LysLeu: 5.721 ± 0.767
2.344LysMet: 2.344 ± 0.436
2.344LysAsn: 2.344 ± 0.435
2.481LysPro: 2.481 ± 0.432
2.895LysGln: 2.895 ± 0.387
2.206LysArg: 2.206 ± 0.472
3.102LysSer: 3.102 ± 0.459
3.86LysThr: 3.86 ± 0.581
3.929LysVal: 3.929 ± 0.541
0.414LysTrp: 0.414 ± 0.151
1.379LysTyr: 1.379 ± 0.298
0.0LysXaa: 0.0 ± 0.0
Leu
7.237LeuAla: 7.237 ± 0.743
1.379LeuCys: 1.379 ± 0.374
4.963LeuAsp: 4.963 ± 0.668
5.79LeuGlu: 5.79 ± 0.746
2.964LeuPhe: 2.964 ± 0.49
5.721LeuGly: 5.721 ± 0.664
1.379LeuHis: 1.379 ± 0.392
3.722LeuIle: 3.722 ± 0.58
5.032LeuLys: 5.032 ± 0.636
5.79LeuLeu: 5.79 ± 0.698
2.55LeuMet: 2.55 ± 0.384
3.653LeuAsn: 3.653 ± 0.358
3.309LeuPro: 3.309 ± 0.559
3.377LeuGln: 3.377 ± 0.637
3.791LeuArg: 3.791 ± 0.58
5.307LeuSer: 5.307 ± 0.547
5.101LeuThr: 5.101 ± 0.562
4.963LeuVal: 4.963 ± 0.653
0.827LeuTrp: 0.827 ± 0.21
2.137LeuTyr: 2.137 ± 0.285
0.0LeuXaa: 0.0 ± 0.0
Met
2.895MetAla: 2.895 ± 0.456
0.482MetCys: 0.482 ± 0.165
1.241MetAsp: 1.241 ± 0.347
1.654MetGlu: 1.654 ± 0.362
0.896MetPhe: 0.896 ± 0.277
1.31MetGly: 1.31 ± 0.279
0.482MetHis: 0.482 ± 0.162
1.447MetIle: 1.447 ± 0.303
1.999MetLys: 1.999 ± 0.419
1.999MetLeu: 1.999 ± 0.284
0.414MetMet: 0.414 ± 0.127
1.034MetAsn: 1.034 ± 0.279
1.103MetPro: 1.103 ± 0.235
1.516MetGln: 1.516 ± 0.3
1.516MetArg: 1.516 ± 0.291
2.481MetSer: 2.481 ± 0.53
1.447MetThr: 1.447 ± 0.311
1.792MetVal: 1.792 ± 0.317
0.62MetTrp: 0.62 ± 0.244
0.827MetTyr: 0.827 ± 0.237
0.0MetXaa: 0.0 ± 0.0
Asn
4.48AsnAla: 4.48 ± 0.737
0.482AsnCys: 0.482 ± 0.2
1.999AsnAsp: 1.999 ± 0.404
2.275AsnGlu: 2.275 ± 0.359
1.93AsnPhe: 1.93 ± 0.366
4.342AsnGly: 4.342 ± 0.666
0.758AsnHis: 0.758 ± 0.225
3.033AsnIle: 3.033 ± 0.505
2.826AsnLys: 2.826 ± 0.382
3.86AsnLeu: 3.86 ± 0.36
1.379AsnMet: 1.379 ± 0.294
2.619AsnAsn: 2.619 ± 0.375
3.309AsnPro: 3.309 ± 0.499
1.654AsnGln: 1.654 ± 0.356
1.861AsnArg: 1.861 ± 0.386
2.964AsnSer: 2.964 ± 0.496
2.481AsnThr: 2.481 ± 0.412
3.102AsnVal: 3.102 ± 0.432
0.551AsnTrp: 0.551 ± 0.158
1.31AsnTyr: 1.31 ± 0.285
0.0AsnXaa: 0.0 ± 0.0
Pro
3.86ProAla: 3.86 ± 0.434
0.414ProCys: 0.414 ± 0.148
2.964ProAsp: 2.964 ± 0.452
3.033ProGlu: 3.033 ± 0.418
0.827ProPhe: 0.827 ± 0.255
1.723ProGly: 1.723 ± 0.33
0.827ProHis: 0.827 ± 0.277
1.999ProIle: 1.999 ± 0.362
2.206ProLys: 2.206 ± 0.402
2.481ProLeu: 2.481 ± 0.45
1.103ProMet: 1.103 ± 0.289
1.792ProAsn: 1.792 ± 0.444
0.965ProPro: 0.965 ± 0.243
1.792ProGln: 1.792 ± 0.413
1.585ProArg: 1.585 ± 0.323
2.619ProSer: 2.619 ± 0.384
2.688ProThr: 2.688 ± 0.495
2.688ProVal: 2.688 ± 0.388
0.62ProTrp: 0.62 ± 0.233
1.31ProTyr: 1.31 ± 0.349
0.0ProXaa: 0.0 ± 0.0
Gln
3.584GlnAla: 3.584 ± 0.445
0.276GlnCys: 0.276 ± 0.149
2.344GlnAsp: 2.344 ± 0.5
2.344GlnGlu: 2.344 ± 0.36
1.654GlnPhe: 1.654 ± 0.338
3.033GlnGly: 3.033 ± 0.459
0.689GlnHis: 0.689 ± 0.233
2.55GlnIle: 2.55 ± 0.43
1.861GlnLys: 1.861 ± 0.445
4.136GlnLeu: 4.136 ± 0.619
1.447GlnMet: 1.447 ± 0.306
1.516GlnAsn: 1.516 ± 0.303
1.516GlnPro: 1.516 ± 0.303
2.344GlnGln: 2.344 ± 0.711
2.344GlnArg: 2.344 ± 0.456
2.206GlnSer: 2.206 ± 0.398
3.033GlnThr: 3.033 ± 0.367
2.55GlnVal: 2.55 ± 0.416
0.551GlnTrp: 0.551 ± 0.15
1.999GlnTyr: 1.999 ± 0.326
0.0GlnXaa: 0.0 ± 0.0
Arg
3.309ArgAla: 3.309 ± 0.599
0.758ArgCys: 0.758 ± 0.278
3.033ArgAsp: 3.033 ± 0.408
3.033ArgGlu: 3.033 ± 0.599
1.723ArgPhe: 1.723 ± 0.474
3.033ArgGly: 3.033 ± 0.588
1.241ArgHis: 1.241 ± 0.276
3.446ArgIle: 3.446 ± 0.538
1.93ArgLys: 1.93 ± 0.35
3.86ArgLeu: 3.86 ± 0.5
0.827ArgMet: 0.827 ± 0.219
2.275ArgAsn: 2.275 ± 0.408
1.654ArgPro: 1.654 ± 0.26
2.275ArgGln: 2.275 ± 0.378
1.861ArgArg: 1.861 ± 0.345
3.584ArgSer: 3.584 ± 0.464
2.275ArgThr: 2.275 ± 0.334
4.48ArgVal: 4.48 ± 0.494
0.758ArgTrp: 0.758 ± 0.259
1.516ArgTyr: 1.516 ± 0.335
0.0ArgXaa: 0.0 ± 0.0
Ser
5.721SerAla: 5.721 ± 0.886
0.276SerCys: 0.276 ± 0.126
3.86SerAsp: 3.86 ± 0.518
3.791SerGlu: 3.791 ± 0.426
2.619SerPhe: 2.619 ± 0.494
6.617SerGly: 6.617 ± 0.775
1.379SerHis: 1.379 ± 0.263
3.998SerIle: 3.998 ± 0.5
3.309SerLys: 3.309 ± 0.474
5.721SerLeu: 5.721 ± 0.548
1.241SerMet: 1.241 ± 0.292
3.24SerAsn: 3.24 ± 0.57
2.757SerPro: 2.757 ± 0.504
2.55SerGln: 2.55 ± 0.445
3.653SerArg: 3.653 ± 0.461
4.274SerSer: 4.274 ± 0.587
3.446SerThr: 3.446 ± 0.563
4.618SerVal: 4.618 ± 0.575
0.758SerTrp: 0.758 ± 0.287
2.344SerTyr: 2.344 ± 0.392
0.0SerXaa: 0.0 ± 0.0
Thr
5.583ThrAla: 5.583 ± 0.761
0.551ThrCys: 0.551 ± 0.215
2.757ThrAsp: 2.757 ± 0.414
3.102ThrGlu: 3.102 ± 0.568
2.688ThrPhe: 2.688 ± 0.429
6.066ThrGly: 6.066 ± 0.667
0.896ThrHis: 0.896 ± 0.218
4.549ThrIle: 4.549 ± 0.526
3.929ThrLys: 3.929 ± 0.515
4.549ThrLeu: 4.549 ± 0.586
1.241ThrMet: 1.241 ± 0.301
3.24ThrAsn: 3.24 ± 0.527
2.344ThrPro: 2.344 ± 0.294
2.068ThrGln: 2.068 ± 0.293
2.412ThrArg: 2.412 ± 0.391
3.929ThrSer: 3.929 ± 0.53
4.136ThrThr: 4.136 ± 0.729
4.342ThrVal: 4.342 ± 0.625
0.827ThrTrp: 0.827 ± 0.276
2.55ThrTyr: 2.55 ± 0.424
0.0ThrXaa: 0.0 ± 0.0
Val
7.1ValAla: 7.1 ± 0.881
0.62ValCys: 0.62 ± 0.273
4.411ValAsp: 4.411 ± 0.632
4.274ValGlu: 4.274 ± 0.698
3.309ValPhe: 3.309 ± 0.544
6.41ValGly: 6.41 ± 0.927
1.241ValHis: 1.241 ± 0.267
4.205ValIle: 4.205 ± 0.556
3.929ValLys: 3.929 ± 0.533
4.825ValLeu: 4.825 ± 0.67
1.723ValMet: 1.723 ± 0.328
3.24ValAsn: 3.24 ± 0.586
3.102ValPro: 3.102 ± 0.382
3.102ValGln: 3.102 ± 0.457
3.584ValArg: 3.584 ± 0.456
4.756ValSer: 4.756 ± 0.776
4.48ValThr: 4.48 ± 0.652
5.445ValVal: 5.445 ± 0.725
1.103ValTrp: 1.103 ± 0.299
1.447ValTyr: 1.447 ± 0.362
0.0ValXaa: 0.0 ± 0.0
Trp
1.034TrpAla: 1.034 ± 0.238
0.414TrpCys: 0.414 ± 0.178
0.896TrpAsp: 0.896 ± 0.265
1.447TrpGlu: 1.447 ± 0.343
0.62TrpPhe: 0.62 ± 0.18
0.758TrpGly: 0.758 ± 0.234
0.276TrpHis: 0.276 ± 0.121
0.551TrpIle: 0.551 ± 0.19
1.103TrpLys: 1.103 ± 0.347
0.551TrpLeu: 0.551 ± 0.159
0.482TrpMet: 0.482 ± 0.206
0.62TrpAsn: 0.62 ± 0.198
0.207TrpPro: 0.207 ± 0.117
0.896TrpGln: 0.896 ± 0.219
0.482TrpArg: 0.482 ± 0.17
1.034TrpSer: 1.034 ± 0.182
0.965TrpThr: 0.965 ± 0.261
0.414TrpVal: 0.414 ± 0.159
0.138TrpTrp: 0.138 ± 0.083
0.758TrpTyr: 0.758 ± 0.202
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.275TyrAla: 2.275 ± 0.387
0.414TyrCys: 0.414 ± 0.161
1.999TyrAsp: 1.999 ± 0.329
1.723TyrGlu: 1.723 ± 0.283
1.241TyrPhe: 1.241 ± 0.255
3.24TyrGly: 3.24 ± 0.431
0.689TyrHis: 0.689 ± 0.303
1.585TyrIle: 1.585 ± 0.349
2.068TyrLys: 2.068 ± 0.349
2.757TyrLeu: 2.757 ± 0.44
1.172TyrMet: 1.172 ± 0.383
1.861TyrAsn: 1.861 ± 0.356
1.31TyrPro: 1.31 ± 0.271
0.965TyrGln: 0.965 ± 0.233
1.447TyrArg: 1.447 ± 0.28
2.688TyrSer: 2.688 ± 0.51
1.723TyrThr: 1.723 ± 0.341
2.481TyrVal: 2.481 ± 0.519
0.276TyrTrp: 0.276 ± 0.144
0.896TyrTyr: 0.896 ± 0.273
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 63 proteins (14509 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski