Nicotiana sylvestris (Wood tobacco) (South American tobacco)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; Nicotiana

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 41327 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U7XA06|A0A1U7XA06_NICSY Isoform of A0A1U7XPS5 uncharacterized protein LOC104236656 isoform X7 OS=Nicotiana sylvestris OX=4096 GN=LOC104236656 PE=4 SV=1
MM1 pKa = 7.35SRR3 pKa = 11.84GIFTKK8 pKa = 10.53AAEE11 pKa = 4.36CDD13 pKa = 3.86SLEE16 pKa = 4.39STSSVPAIDD25 pKa = 3.6IQVGPDD31 pKa = 3.51SIEE34 pKa = 3.9IDD36 pKa = 4.22PIDD39 pKa = 3.93YY40 pKa = 9.76TDD42 pKa = 3.57SATTTLSNKK51 pKa = 7.87EE52 pKa = 4.33DD53 pKa = 3.47KK54 pKa = 11.35VFDD57 pKa = 4.37QISQGIGTVLWYY69 pKa = 10.54HH70 pKa = 6.2SVDD73 pKa = 3.65DD74 pKa = 3.83VTFPISFDD82 pKa = 3.97DD83 pKa = 4.46SPLLPSSVEE92 pKa = 3.85QLNSQVLFPGIEE104 pKa = 4.08SALEE108 pKa = 3.97EE109 pKa = 4.43DD110 pKa = 3.49TTSYY114 pKa = 10.95VNQVVIFQFIKK125 pKa = 10.47CSHH128 pKa = 6.68CLLVSIWMMAICLILLIAFMM148 pKa = 5.36

Molecular weight: 16.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U7X5Y3|A0A1U7X5Y3_NICSY zinc finger protein 4-like OS=Nicotiana sylvestris OX=4096 GN=LOC104233402 PE=4 SV=1
RR1 pKa = 7.68RR2 pKa = 11.84PNNPAQPHH10 pKa = 5.88RR11 pKa = 11.84RR12 pKa = 11.84PPTLPVPASPASRR25 pKa = 11.84PKK27 pKa = 8.29TTSLSPPPSS36 pKa = 3.29

Molecular weight: 3.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

33640

7687

41327

17030923

11

5102

412.1

46.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.538 ± 0.011

1.875 ± 0.005

5.293 ± 0.008

6.63 ± 0.014

4.192 ± 0.009

6.217 ± 0.011

2.368 ± 0.005

5.505 ± 0.008

6.436 ± 0.011

9.624 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.004

4.632 ± 0.01

4.783 ± 0.011

3.832 ± 0.009

5.245 ± 0.009

8.89 ± 0.013

4.905 ± 0.007

6.428 ± 0.008

1.275 ± 0.004

2.891 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski