Cryobacterium psychrotolerans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Cryobacterium

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3047 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G8ZC01|A0A1G8ZC01_9MICO Carboxypeptidase regulatory-like domain-containing protein OS=Cryobacterium psychrotolerans OX=386301 GN=E3T56_06240 PE=4 SV=1
MM1 pKa = 7.12NRR3 pKa = 11.84SASKK7 pKa = 8.84FTMVGLMVSGILFAGTACASNQSGADD33 pKa = 3.49SAAGPAGDD41 pKa = 4.23VAPSSSPGPEE51 pKa = 3.59VDD53 pKa = 3.75PLTSVTTIVVRR64 pKa = 11.84PEE66 pKa = 3.44ALEE69 pKa = 4.55LMDD72 pKa = 4.34EE73 pKa = 4.46SGSLVASLTYY83 pKa = 10.25EE84 pKa = 4.39DD85 pKa = 5.16PLAAMLGALSVVVGEE100 pKa = 4.75EE101 pKa = 3.93PATASYY107 pKa = 10.88EE108 pKa = 4.31GGLEE112 pKa = 3.96RR113 pKa = 11.84PAGQTYY119 pKa = 9.73SWAGLTLTDD128 pKa = 4.01YY129 pKa = 11.04LPPEE133 pKa = 4.39GKK135 pKa = 10.26FLGEE139 pKa = 3.87SDD141 pKa = 3.91YY142 pKa = 11.85SLDD145 pKa = 3.49FTAGQVGDD153 pKa = 3.97GVAITTEE160 pKa = 4.15SGSTVGDD167 pKa = 3.86DD168 pKa = 3.31MDD170 pKa = 4.6AVADD174 pKa = 4.03EE175 pKa = 5.33LGLSVNRR182 pKa = 11.84DD183 pKa = 3.41FASAGHH189 pKa = 7.0LFFLIEE195 pKa = 4.24VGPEE199 pKa = 3.71LTTPAGDD206 pKa = 3.57NIEE209 pKa = 4.58YY210 pKa = 8.98PNAWAVHH217 pKa = 5.08VSSTSGSGVINNIGAPVNLSHH238 pKa = 6.8WVSS241 pKa = 3.29

Molecular weight: 24.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9FNI6|A0A1G9FNI6_9MICO Uncharacterized protein OS=Cryobacterium psychrotolerans OX=386301 GN=SAMN05216282_11727 PE=4 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TAATQTVPPSRR14 pKa = 11.84VTVSVRR20 pKa = 11.84LIAGTPTVHH29 pKa = 7.21SVMASVLLIAATPTGPRR46 pKa = 11.84NRR48 pKa = 11.84ATVNASPGRR57 pKa = 11.84RR58 pKa = 11.84MGSVRR63 pKa = 11.84LTAEE67 pKa = 4.0TPIVRR72 pKa = 11.84SVMASVLLTVATPTVPPSPVSASRR96 pKa = 11.84GRR98 pKa = 11.84RR99 pKa = 11.84MATARR104 pKa = 11.84LTAEE108 pKa = 4.17TPTVRR113 pKa = 11.84SRR115 pKa = 11.84AATAPPTGATRR126 pKa = 11.84TVPRR130 pKa = 11.84RR131 pKa = 11.84RR132 pKa = 11.84VMVSASRR139 pKa = 11.84GRR141 pKa = 11.84RR142 pKa = 11.84MASGLRR148 pKa = 11.84SATLTVRR155 pKa = 11.84PIAPMPRR162 pKa = 11.84VEE164 pKa = 4.4SVRR167 pKa = 11.84SVMVSASPGPRR178 pKa = 11.84MASVPRR184 pKa = 11.84IVATPIVRR192 pKa = 11.84SRR194 pKa = 11.84VASVPRR200 pKa = 11.84TGEE203 pKa = 3.87TRR205 pKa = 11.84TVPPSRR211 pKa = 11.84VTASASPGPGMVPRR225 pKa = 11.84PSARR229 pKa = 11.84AVTLLPVTGNARR241 pKa = 11.84SGAHH245 pKa = 5.25AAVRR249 pKa = 11.84RR250 pKa = 11.84PVPAPPVTPEE260 pKa = 4.17KK261 pKa = 10.92SSGPAKK267 pKa = 10.36APPPAVTATRR277 pKa = 11.84APSKK281 pKa = 10.57RR282 pKa = 11.84RR283 pKa = 3.41

Molecular weight: 29.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3047

0

3047

978152

25

2218

321.0

34.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.252 ± 0.064

0.533 ± 0.011

5.842 ± 0.038

5.457 ± 0.04

3.179 ± 0.025

9.053 ± 0.039

1.985 ± 0.022

4.723 ± 0.032

2.287 ± 0.03

10.438 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.845 ± 0.017

2.219 ± 0.026

5.382 ± 0.029

2.802 ± 0.021

6.986 ± 0.044

5.856 ± 0.03

6.075 ± 0.046

8.645 ± 0.042

1.413 ± 0.018

2.025 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski