[Clostridium] fimetarium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Lachnoclostridium

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4150 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0MVE7|A0A1I0MVE7_9FIRM Arabinogalactan oligomer / maltooligosaccharide transport system permease protein OS=[Clostridium] fimetarium OX=99656 GN=SAMN05421659_102157 PE=3 SV=1
MM1 pKa = 7.49NKK3 pKa = 10.48GIDD6 pKa = 3.46VSSNNGTLDD15 pKa = 2.84WGAIKK20 pKa = 10.65ADD22 pKa = 4.78GIQFAIIRR30 pKa = 11.84IGYY33 pKa = 9.95GDD35 pKa = 4.92DD36 pKa = 4.37EE37 pKa = 4.97VNQDD41 pKa = 4.3DD42 pKa = 4.27AQANANMDD50 pKa = 3.31ACQNLGIPYY59 pKa = 9.75GVYY62 pKa = 9.38IYY64 pKa = 10.69SYY66 pKa = 10.21AISEE70 pKa = 4.37NEE72 pKa = 4.0VKK74 pKa = 10.75SEE76 pKa = 3.81VRR78 pKa = 11.84HH79 pKa = 4.75TLRR82 pKa = 11.84MIEE85 pKa = 3.96GRR87 pKa = 11.84NPQLGVFIDD96 pKa = 4.01MEE98 pKa = 5.35DD99 pKa = 3.32SDD101 pKa = 5.04GYY103 pKa = 8.89KK104 pKa = 9.37TRR106 pKa = 11.84HH107 pKa = 5.52NLVPEE112 pKa = 4.12EE113 pKa = 4.09NGAILTNFCCIYY125 pKa = 10.55CNDD128 pKa = 4.0MTNSGYY134 pKa = 10.71KK135 pKa = 10.14AGIYY139 pKa = 10.05ANKK142 pKa = 10.48NYY144 pKa = 9.89FDD146 pKa = 5.25NILDD150 pKa = 3.41ISRR153 pKa = 11.84FDD155 pKa = 5.58SYY157 pKa = 11.03MKK159 pKa = 10.01WLAIWGPDD167 pKa = 3.24QCPAGNWILWQYY179 pKa = 9.76TSDD182 pKa = 3.47GVVQGSSARR191 pKa = 11.84TDD193 pKa = 2.62MDD195 pKa = 3.63YY196 pKa = 11.4YY197 pKa = 11.19YY198 pKa = 11.48GEE200 pKa = 4.44FPNTEE205 pKa = 4.3PEE207 pKa = 4.27PEE209 pKa = 4.41PEE211 pKa = 4.41PEE213 pKa = 4.41PEE215 pKa = 4.41PEE217 pKa = 4.41PEE219 pKa = 4.41PEE221 pKa = 4.41PEE223 pKa = 4.41PEE225 pKa = 4.41PEE227 pKa = 4.41PEE229 pKa = 4.41PEE231 pKa = 4.41PEE233 pKa = 4.41PEE235 pKa = 4.41PEE237 pKa = 4.41PEE239 pKa = 4.41PEE241 pKa = 4.41PEE243 pKa = 4.41PEE245 pKa = 4.41PEE247 pKa = 4.41PEE249 pKa = 4.41PEE251 pKa = 4.41PEE253 pKa = 4.41PEE255 pKa = 4.41PEE257 pKa = 4.41PEE259 pKa = 4.41PEE261 pKa = 4.41PEE263 pKa = 4.41PEE265 pKa = 4.41PEE267 pKa = 4.41PEE269 pKa = 4.41PEE271 pKa = 4.29PEE273 pKa = 4.1PEE275 pKa = 3.82PVKK278 pKa = 10.57KK279 pKa = 10.48SKK281 pKa = 10.85CFFLMIITNIVNWFKK296 pKa = 11.09GIHH299 pKa = 5.02FF300 pKa = 4.82

Molecular weight: 34.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0QTC6|A0A1I0QTC6_9FIRM Ribosomal protein S18 acetylase RimI OS=[Clostridium] fimetarium OX=99656 GN=SAMN05421659_10995 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 9.06KK9 pKa = 8.03RR10 pKa = 11.84SRR12 pKa = 11.84SKK14 pKa = 9.44VHH16 pKa = 6.17GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84RR29 pKa = 11.84VITARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.99GRR39 pKa = 11.84KK40 pKa = 8.91SLSAA44 pKa = 3.86

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4150

0

4150

1311489

29

3424

316.0

35.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.326 ± 0.042

1.38 ± 0.014

5.618 ± 0.03

6.746 ± 0.045

4.384 ± 0.028

6.359 ± 0.04

1.467 ± 0.015

9.414 ± 0.051

7.737 ± 0.037

8.637 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.919 ± 0.019

5.897 ± 0.03

2.774 ± 0.023

2.965 ± 0.017

3.278 ± 0.026

6.602 ± 0.034

5.681 ± 0.054

6.655 ± 0.031

0.797 ± 0.012

4.362 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski