Amino acid dipepetide frequency for Staphylococcus phage IME1367_01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.696AlaAla: 1.696 ± 0.415
0.231AlaCys: 0.231 ± 0.123
2.39AlaAsp: 2.39 ± 0.333
3.084AlaGlu: 3.084 ± 0.359
2.775AlaPhe: 2.775 ± 0.834
3.007AlaGly: 3.007 ± 0.71
0.925AlaHis: 0.925 ± 0.266
5.011AlaIle: 5.011 ± 1.005
5.242AlaLys: 5.242 ± 0.699
5.165AlaLeu: 5.165 ± 0.839
1.311AlaMet: 1.311 ± 0.335
2.93AlaAsn: 2.93 ± 0.435
0.925AlaPro: 0.925 ± 0.234
3.392AlaGln: 3.392 ± 0.479
2.698AlaArg: 2.698 ± 0.428
4.086AlaSer: 4.086 ± 0.803
3.238AlaThr: 3.238 ± 0.51
3.546AlaVal: 3.546 ± 0.562
0.925AlaTrp: 0.925 ± 0.384
1.619AlaTyr: 1.619 ± 0.381
0.0AlaXaa: 0.0 ± 0.0
Cys
0.231CysAla: 0.231 ± 0.13
0.0CysCys: 0.0 ± 0.0
0.231CysAsp: 0.231 ± 0.15
0.54CysGlu: 0.54 ± 0.273
0.0CysPhe: 0.0 ± 0.0
0.308CysGly: 0.308 ± 0.173
0.231CysHis: 0.231 ± 0.176
0.617CysIle: 0.617 ± 0.237
0.385CysLys: 0.385 ± 0.186
0.385CysLeu: 0.385 ± 0.166
0.154CysMet: 0.154 ± 0.094
0.385CysAsn: 0.385 ± 0.18
0.231CysPro: 0.231 ± 0.151
0.154CysGln: 0.154 ± 0.117
0.54CysArg: 0.54 ± 0.207
0.463CysSer: 0.463 ± 0.197
0.385CysThr: 0.385 ± 0.151
0.54CysVal: 0.54 ± 0.177
0.077CysTrp: 0.077 ± 0.081
0.463CysTyr: 0.463 ± 0.189
0.0CysXaa: 0.0 ± 0.0
Asp
2.39AspAla: 2.39 ± 0.364
0.231AspCys: 0.231 ± 0.168
4.394AspAsp: 4.394 ± 0.866
5.936AspGlu: 5.936 ± 0.96
3.084AspPhe: 3.084 ± 0.693
3.932AspGly: 3.932 ± 0.493
0.385AspHis: 0.385 ± 0.157
5.011AspIle: 5.011 ± 0.72
7.17AspLys: 7.17 ± 0.74
4.24AspLeu: 4.24 ± 0.652
1.85AspMet: 1.85 ± 0.375
4.394AspAsn: 4.394 ± 0.674
1.311AspPro: 1.311 ± 0.389
1.079AspGln: 1.079 ± 0.286
1.773AspArg: 1.773 ± 0.332
3.623AspSer: 3.623 ± 0.55
4.703AspThr: 4.703 ± 0.987
3.932AspVal: 3.932 ± 0.692
0.925AspTrp: 0.925 ± 0.266
2.621AspTyr: 2.621 ± 0.516
0.0AspXaa: 0.0 ± 0.0
Glu
2.004GluAla: 2.004 ± 0.387
0.848GluCys: 0.848 ± 0.314
3.855GluAsp: 3.855 ± 0.577
4.626GluGlu: 4.626 ± 0.815
3.161GluPhe: 3.161 ± 0.635
2.621GluGly: 2.621 ± 0.408
0.925GluHis: 0.925 ± 0.292
5.165GluIle: 5.165 ± 0.901
6.476GluLys: 6.476 ± 0.874
7.401GluLeu: 7.401 ± 0.978
2.93GluMet: 2.93 ± 0.402
4.549GluAsn: 4.549 ± 0.773
1.85GluPro: 1.85 ± 0.507
3.315GluGln: 3.315 ± 0.486
3.469GluArg: 3.469 ± 0.453
3.238GluSer: 3.238 ± 0.4
3.932GluThr: 3.932 ± 0.51
4.394GluVal: 4.394 ± 0.486
0.925GluTrp: 0.925 ± 0.315
3.623GluTyr: 3.623 ± 0.62
0.0GluXaa: 0.0 ± 0.0
Phe
3.007PheAla: 3.007 ± 0.888
0.308PheCys: 0.308 ± 0.181
2.698PheAsp: 2.698 ± 0.442
2.698PheGlu: 2.698 ± 0.516
1.002PhePhe: 1.002 ± 0.277
3.315PheGly: 3.315 ± 0.466
0.463PheHis: 0.463 ± 0.169
3.007PheIle: 3.007 ± 0.629
4.086PheLys: 4.086 ± 0.694
2.544PheLeu: 2.544 ± 0.389
1.002PheMet: 1.002 ± 0.273
4.086PheAsn: 4.086 ± 0.557
0.54PhePro: 0.54 ± 0.202
1.079PheGln: 1.079 ± 0.243
1.311PheArg: 1.311 ± 0.304
1.85PheSer: 1.85 ± 0.415
2.698PheThr: 2.698 ± 0.501
2.39PheVal: 2.39 ± 0.409
0.231PheTrp: 0.231 ± 0.157
1.85PheTyr: 1.85 ± 0.438
0.0PheXaa: 0.0 ± 0.0
Gly
4.317GlyAla: 4.317 ± 1.397
0.231GlyCys: 0.231 ± 0.133
2.698GlyAsp: 2.698 ± 0.514
2.775GlyGlu: 2.775 ± 0.574
2.698GlyPhe: 2.698 ± 0.584
3.623GlyGly: 3.623 ± 1.338
1.619GlyHis: 1.619 ± 0.418
4.78GlyIle: 4.78 ± 0.891
4.857GlyLys: 4.857 ± 0.52
5.397GlyLeu: 5.397 ± 1.048
1.927GlyMet: 1.927 ± 0.409
3.546GlyAsn: 3.546 ± 0.47
0.694GlyPro: 0.694 ± 0.325
2.621GlyGln: 2.621 ± 0.485
3.084GlyArg: 3.084 ± 0.501
3.392GlySer: 3.392 ± 0.575
4.163GlyThr: 4.163 ± 0.583
4.549GlyVal: 4.549 ± 0.961
1.002GlyTrp: 1.002 ± 0.378
2.621GlyTyr: 2.621 ± 0.38
0.0GlyXaa: 0.0 ± 0.0
His
0.925HisAla: 0.925 ± 0.231
0.154HisCys: 0.154 ± 0.105
1.156HisAsp: 1.156 ± 0.253
0.848HisGlu: 0.848 ± 0.218
0.617HisPhe: 0.617 ± 0.252
1.002HisGly: 1.002 ± 0.268
0.54HisHis: 0.54 ± 0.202
1.465HisIle: 1.465 ± 0.349
1.619HisLys: 1.619 ± 0.376
1.542HisLeu: 1.542 ± 0.319
0.54HisMet: 0.54 ± 0.211
1.388HisAsn: 1.388 ± 0.283
0.54HisPro: 0.54 ± 0.241
0.771HisGln: 0.771 ± 0.254
0.925HisArg: 0.925 ± 0.282
1.388HisSer: 1.388 ± 0.288
1.002HisThr: 1.002 ± 0.21
1.002HisVal: 1.002 ± 0.338
0.154HisTrp: 0.154 ± 0.11
1.079HisTyr: 1.079 ± 0.245
0.0HisXaa: 0.0 ± 0.0
Ile
4.78IleAla: 4.78 ± 0.589
0.771IleCys: 0.771 ± 0.294
4.78IleAsp: 4.78 ± 0.596
6.091IleGlu: 6.091 ± 0.958
2.698IlePhe: 2.698 ± 0.438
4.086IleGly: 4.086 ± 0.777
1.465IleHis: 1.465 ± 0.322
4.78IleIle: 4.78 ± 0.549
7.401IleLys: 7.401 ± 0.828
3.546IleLeu: 3.546 ± 0.599
1.079IleMet: 1.079 ± 0.264
5.551IleAsn: 5.551 ± 0.788
2.159IlePro: 2.159 ± 0.482
2.621IleGln: 2.621 ± 0.545
2.544IleArg: 2.544 ± 0.381
4.934IleSer: 4.934 ± 0.843
3.932IleThr: 3.932 ± 0.658
3.701IleVal: 3.701 ± 0.393
0.848IleTrp: 0.848 ± 0.279
2.93IleTyr: 2.93 ± 0.538
0.0IleXaa: 0.0 ± 0.0
Lys
5.165LysAla: 5.165 ± 0.557
0.463LysCys: 0.463 ± 0.223
6.091LysAsp: 6.091 ± 0.799
6.168LysGlu: 6.168 ± 0.89
3.469LysPhe: 3.469 ± 0.471
7.016LysGly: 7.016 ± 0.73
1.927LysHis: 1.927 ± 0.35
4.857LysIle: 4.857 ± 0.75
6.476LysLys: 6.476 ± 0.87
7.71LysLeu: 7.71 ± 0.678
2.236LysMet: 2.236 ± 0.47
6.168LysAsn: 6.168 ± 0.903
2.467LysPro: 2.467 ± 0.397
5.397LysGln: 5.397 ± 0.682
4.626LysArg: 4.626 ± 0.665
5.242LysSer: 5.242 ± 0.672
4.78LysThr: 4.78 ± 0.555
6.091LysVal: 6.091 ± 0.55
1.234LysTrp: 1.234 ± 0.29
3.932LysTyr: 3.932 ± 0.457
0.0LysXaa: 0.0 ± 0.0
Leu
4.163LeuAla: 4.163 ± 0.563
0.463LeuCys: 0.463 ± 0.161
5.551LeuAsp: 5.551 ± 0.973
5.628LeuGlu: 5.628 ± 0.74
2.467LeuPhe: 2.467 ± 0.384
4.009LeuGly: 4.009 ± 0.792
1.465LeuHis: 1.465 ± 0.319
4.626LeuIle: 4.626 ± 0.599
8.403LeuLys: 8.403 ± 0.908
5.011LeuLeu: 5.011 ± 0.743
2.082LeuMet: 2.082 ± 0.373
6.63LeuAsn: 6.63 ± 0.888
2.621LeuPro: 2.621 ± 0.42
3.007LeuGln: 3.007 ± 0.546
3.623LeuArg: 3.623 ± 0.622
5.397LeuSer: 5.397 ± 0.776
5.32LeuThr: 5.32 ± 0.523
3.701LeuVal: 3.701 ± 0.469
0.771LeuTrp: 0.771 ± 0.307
3.546LeuTyr: 3.546 ± 0.543
0.0LeuXaa: 0.0 ± 0.0
Met
2.159MetAla: 2.159 ± 0.44
0.077MetCys: 0.077 ± 0.074
1.079MetAsp: 1.079 ± 0.234
1.619MetGlu: 1.619 ± 0.507
0.925MetPhe: 0.925 ± 0.295
0.848MetGly: 0.848 ± 0.25
0.54MetHis: 0.54 ± 0.218
1.465MetIle: 1.465 ± 0.336
2.313MetLys: 2.313 ± 0.461
2.39MetLeu: 2.39 ± 0.376
0.694MetMet: 0.694 ± 0.232
2.082MetAsn: 2.082 ± 0.447
0.694MetPro: 0.694 ± 0.171
1.234MetGln: 1.234 ± 0.3
1.156MetArg: 1.156 ± 0.256
2.004MetSer: 2.004 ± 0.325
2.621MetThr: 2.621 ± 0.569
0.925MetVal: 0.925 ± 0.299
0.463MetTrp: 0.463 ± 0.212
0.308MetTyr: 0.308 ± 0.192
0.0MetXaa: 0.0 ± 0.0
Asn
4.24AsnAla: 4.24 ± 0.512
0.463AsnCys: 0.463 ± 0.184
4.934AsnAsp: 4.934 ± 0.793
5.088AsnGlu: 5.088 ± 0.754
2.544AsnPhe: 2.544 ± 0.395
6.861AsnGly: 6.861 ± 1.054
1.156AsnHis: 1.156 ± 0.292
3.392AsnIle: 3.392 ± 0.53
6.553AsnLys: 6.553 ± 0.706
5.551AsnLeu: 5.551 ± 0.636
2.313AsnMet: 2.313 ± 0.421
6.322AsnAsn: 6.322 ± 1.062
2.004AsnPro: 2.004 ± 0.32
2.775AsnGln: 2.775 ± 0.425
2.775AsnArg: 2.775 ± 0.398
3.778AsnSer: 3.778 ± 0.55
4.163AsnThr: 4.163 ± 0.616
3.623AsnVal: 3.623 ± 0.685
0.771AsnTrp: 0.771 ± 0.197
3.007AsnTyr: 3.007 ± 0.402
0.0AsnXaa: 0.0 ± 0.0
Pro
1.002ProAla: 1.002 ± 0.284
0.0ProCys: 0.0 ± 0.0
1.079ProAsp: 1.079 ± 0.329
1.85ProGlu: 1.85 ± 0.535
1.542ProPhe: 1.542 ± 0.391
1.311ProGly: 1.311 ± 0.336
0.308ProHis: 0.308 ± 0.174
2.39ProIle: 2.39 ± 0.401
2.39ProLys: 2.39 ± 0.399
1.156ProLeu: 1.156 ± 0.342
0.694ProMet: 0.694 ± 0.211
2.39ProAsn: 2.39 ± 0.385
1.002ProPro: 1.002 ± 0.287
1.311ProGln: 1.311 ± 0.366
0.617ProArg: 0.617 ± 0.246
1.465ProSer: 1.465 ± 0.389
1.85ProThr: 1.85 ± 0.374
2.159ProVal: 2.159 ± 0.404
0.154ProTrp: 0.154 ± 0.09
1.156ProTyr: 1.156 ± 0.271
0.0ProXaa: 0.0 ± 0.0
Gln
3.315GlnAla: 3.315 ± 0.576
0.463GlnCys: 0.463 ± 0.182
2.93GlnAsp: 2.93 ± 0.448
3.161GlnGlu: 3.161 ± 0.527
1.619GlnPhe: 1.619 ± 0.321
2.004GlnGly: 2.004 ± 0.437
1.002GlnHis: 1.002 ± 0.333
2.93GlnIle: 2.93 ± 0.502
2.853GlnLys: 2.853 ± 0.393
3.238GlnLeu: 3.238 ± 0.6
0.54GlnMet: 0.54 ± 0.232
2.082GlnAsn: 2.082 ± 0.33
1.156GlnPro: 1.156 ± 0.368
1.927GlnGln: 1.927 ± 0.329
2.544GlnArg: 2.544 ± 0.458
2.236GlnSer: 2.236 ± 0.38
2.082GlnThr: 2.082 ± 0.418
2.313GlnVal: 2.313 ± 0.494
0.617GlnTrp: 0.617 ± 0.216
1.927GlnTyr: 1.927 ± 0.349
0.0GlnXaa: 0.0 ± 0.0
Arg
2.313ArgAla: 2.313 ± 0.837
0.385ArgCys: 0.385 ± 0.171
3.007ArgAsp: 3.007 ± 0.497
2.698ArgGlu: 2.698 ± 0.54
1.234ArgPhe: 1.234 ± 0.264
2.159ArgGly: 2.159 ± 0.505
1.002ArgHis: 1.002 ± 0.282
3.238ArgIle: 3.238 ± 0.564
4.086ArgLys: 4.086 ± 0.694
3.855ArgLeu: 3.855 ± 0.646
1.311ArgMet: 1.311 ± 0.329
3.315ArgAsn: 3.315 ± 0.566
1.388ArgPro: 1.388 ± 0.335
1.388ArgGln: 1.388 ± 0.386
1.388ArgArg: 1.388 ± 0.318
2.004ArgSer: 2.004 ± 0.408
2.775ArgThr: 2.775 ± 0.455
2.39ArgVal: 2.39 ± 0.386
0.848ArgTrp: 0.848 ± 0.27
3.161ArgTyr: 3.161 ± 0.518
0.0ArgXaa: 0.0 ± 0.0
Ser
3.007SerAla: 3.007 ± 0.665
0.154SerCys: 0.154 ± 0.099
4.934SerAsp: 4.934 ± 0.704
3.392SerGlu: 3.392 ± 0.465
2.775SerPhe: 2.775 ± 0.496
3.778SerGly: 3.778 ± 0.962
1.696SerHis: 1.696 ± 0.285
4.394SerIle: 4.394 ± 0.492
5.32SerLys: 5.32 ± 0.582
4.78SerLeu: 4.78 ± 0.61
1.619SerMet: 1.619 ± 0.391
4.163SerAsn: 4.163 ± 0.928
1.388SerPro: 1.388 ± 0.27
2.467SerGln: 2.467 ± 0.342
2.467SerArg: 2.467 ± 0.382
2.853SerSer: 2.853 ± 0.584
3.469SerThr: 3.469 ± 0.481
3.701SerVal: 3.701 ± 0.603
0.848SerTrp: 0.848 ± 0.209
2.775SerTyr: 2.775 ± 0.602
0.0SerXaa: 0.0 ± 0.0
Thr
3.546ThrAla: 3.546 ± 0.601
0.231ThrCys: 0.231 ± 0.133
3.315ThrAsp: 3.315 ± 0.863
3.623ThrGlu: 3.623 ± 0.697
3.084ThrPhe: 3.084 ± 0.404
4.703ThrGly: 4.703 ± 0.608
1.079ThrHis: 1.079 ± 0.351
3.546ThrIle: 3.546 ± 0.43
4.549ThrLys: 4.549 ± 0.452
5.859ThrLeu: 5.859 ± 0.593
1.311ThrMet: 1.311 ± 0.244
4.24ThrAsn: 4.24 ± 0.63
1.85ThrPro: 1.85 ± 0.411
2.159ThrGln: 2.159 ± 0.298
2.004ThrArg: 2.004 ± 0.442
4.086ThrSer: 4.086 ± 0.57
3.932ThrThr: 3.932 ± 0.503
4.24ThrVal: 4.24 ± 0.59
1.079ThrTrp: 1.079 ± 0.42
2.775ThrTyr: 2.775 ± 0.453
0.0ThrXaa: 0.0 ± 0.0
Val
2.93ValAla: 2.93 ± 0.583
0.231ValCys: 0.231 ± 0.132
4.703ValAsp: 4.703 ± 0.679
4.626ValGlu: 4.626 ± 0.947
2.004ValPhe: 2.004 ± 0.427
3.161ValGly: 3.161 ± 0.586
0.848ValHis: 0.848 ± 0.272
5.088ValIle: 5.088 ± 0.677
5.782ValLys: 5.782 ± 0.857
4.009ValLeu: 4.009 ± 0.622
1.234ValMet: 1.234 ± 0.32
3.546ValAsn: 3.546 ± 0.531
1.696ValPro: 1.696 ± 0.456
1.619ValGln: 1.619 ± 0.334
3.392ValArg: 3.392 ± 0.427
5.011ValSer: 5.011 ± 0.612
3.469ValThr: 3.469 ± 0.439
3.469ValVal: 3.469 ± 0.569
0.848ValTrp: 0.848 ± 0.439
2.236ValTyr: 2.236 ± 0.321
0.0ValXaa: 0.0 ± 0.0
Trp
0.848TrpAla: 0.848 ± 0.268
0.154TrpCys: 0.154 ± 0.113
0.54TrpAsp: 0.54 ± 0.182
0.463TrpGlu: 0.463 ± 0.188
0.694TrpPhe: 0.694 ± 0.231
0.771TrpGly: 0.771 ± 0.251
0.231TrpHis: 0.231 ± 0.179
1.156TrpIle: 1.156 ± 0.292
1.311TrpLys: 1.311 ± 0.357
1.079TrpLeu: 1.079 ± 0.276
0.077TrpMet: 0.077 ± 0.085
2.159TrpAsn: 2.159 ± 0.95
0.154TrpPro: 0.154 ± 0.102
0.54TrpGln: 0.54 ± 0.183
0.617TrpArg: 0.617 ± 0.192
0.925TrpSer: 0.925 ± 0.383
0.308TrpThr: 0.308 ± 0.14
0.925TrpVal: 0.925 ± 0.22
0.154TrpTrp: 0.154 ± 0.127
0.54TrpTyr: 0.54 ± 0.21
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.159TyrAla: 2.159 ± 0.409
0.463TyrCys: 0.463 ± 0.229
2.621TyrAsp: 2.621 ± 0.634
4.394TyrGlu: 4.394 ± 0.739
1.773TyrPhe: 1.773 ± 0.398
2.082TyrGly: 2.082 ± 0.361
0.848TyrHis: 0.848 ± 0.27
3.778TyrIle: 3.778 ± 0.579
4.24TyrLys: 4.24 ± 0.576
3.701TyrLeu: 3.701 ± 0.542
0.617TyrMet: 0.617 ± 0.185
2.39TyrAsn: 2.39 ± 0.437
1.156TyrPro: 1.156 ± 0.323
2.082TyrGln: 2.082 ± 0.359
2.39TyrArg: 2.39 ± 0.464
2.004TyrSer: 2.004 ± 0.317
2.39TyrThr: 2.39 ± 0.455
2.313TyrVal: 2.313 ± 0.459
0.771TyrTrp: 0.771 ± 0.256
1.542TyrTyr: 1.542 ± 0.413
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (12972 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski