Primorskyibacter sp. SS33

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Primorskyibacter; unclassified Primorskyibacter

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3259 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6APE9|A0A4R6APE9_9RHOB Glycosyltransferase OS=Primorskyibacter sp. SS33 OX=2547833 GN=E2L08_02385 PE=4 SV=1
MM1 pKa = 7.55KK2 pKa = 8.88PTMFTTTAGLALCLGAGAALAQEE25 pKa = 4.31VTLTVEE31 pKa = 4.12SWRR34 pKa = 11.84SEE36 pKa = 3.95DD37 pKa = 3.35QAIWNDD43 pKa = 3.49TLIPAFEE50 pKa = 4.52EE51 pKa = 3.77KK52 pKa = 10.66HH53 pKa = 5.73PDD55 pKa = 2.93INIEE59 pKa = 4.11FTPTAPTEE67 pKa = 3.95YY68 pKa = 10.48NAALNARR75 pKa = 11.84LEE77 pKa = 4.43GGTAGDD83 pKa = 4.3LVTCRR88 pKa = 11.84SFDD91 pKa = 3.26GSLPLFNKK99 pKa = 9.94GQLADD104 pKa = 3.82ISDD107 pKa = 4.59LDD109 pKa = 3.97GLSNFPDD116 pKa = 4.73FARR119 pKa = 11.84AAWSTDD125 pKa = 2.91DD126 pKa = 4.13GASTFCVPMASVIHH140 pKa = 5.47GFIYY144 pKa = 10.7NADD147 pKa = 3.44AFAEE151 pKa = 4.38LGLEE155 pKa = 4.09EE156 pKa = 5.35PEE158 pKa = 4.31TVDD161 pKa = 4.01EE162 pKa = 5.24FFAVLDD168 pKa = 4.77AIKK171 pKa = 10.44EE172 pKa = 4.13DD173 pKa = 3.69GSYY176 pKa = 10.6IPLAMGSADD185 pKa = 3.15QWEE188 pKa = 4.8AATMAYY194 pKa = 9.69TNIGPNYY201 pKa = 8.24WKK203 pKa = 10.85GEE205 pKa = 4.12EE206 pKa = 4.34GRR208 pKa = 11.84QALIDD213 pKa = 3.53GTAAFTDD220 pKa = 4.01PQFVAPYY227 pKa = 8.69EE228 pKa = 4.1QMVRR232 pKa = 11.84WTDD235 pKa = 3.42YY236 pKa = 10.29MGPAYY241 pKa = 9.74QAQGYY246 pKa = 9.82SDD248 pKa = 3.84SQNLFTLGRR257 pKa = 11.84AAIYY261 pKa = 8.44PAGSWEE267 pKa = 3.81ITGFTEE273 pKa = 4.83AADD276 pKa = 4.02FEE278 pKa = 4.89MGAFPPPVQNAGDD291 pKa = 3.38TCYY294 pKa = 10.68ISDD297 pKa = 4.01HH298 pKa = 6.0TDD300 pKa = 2.37IGLGMNAATDD310 pKa = 3.65HH311 pKa = 6.98PEE313 pKa = 3.65AARR316 pKa = 11.84TFLEE320 pKa = 4.7WVASPEE326 pKa = 3.92FAEE329 pKa = 5.43LYY331 pKa = 10.16TNALPGFFSLSDD343 pKa = 3.36AQFEE347 pKa = 4.49VDD349 pKa = 4.6NPLAQEE355 pKa = 4.43FIEE358 pKa = 4.36FRR360 pKa = 11.84EE361 pKa = 4.25NCEE364 pKa = 3.73STVRR368 pKa = 11.84VASQILSRR376 pKa = 11.84GEE378 pKa = 3.78PNTWNNMWVLSANVLNDD395 pKa = 3.09AATPEE400 pKa = 4.15EE401 pKa = 4.38ATAEE405 pKa = 4.11LQQGLASWYY414 pKa = 9.83EE415 pKa = 3.92PQQNN419 pKa = 3.13

Molecular weight: 45.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6AL34|A0A4R6AL34_9RHOB LysR family transcriptional regulator OS=Primorskyibacter sp. SS33 OX=2547833 GN=E2L08_01260 PE=3 SV=1
MM1 pKa = 7.54NLGQIGTMAVRR12 pKa = 11.84MILRR16 pKa = 11.84RR17 pKa = 11.84LMSRR21 pKa = 11.84GMNAGINRR29 pKa = 11.84AFGAARR35 pKa = 11.84TPGAAKK41 pKa = 9.91QGRR44 pKa = 11.84AAQVSGKK51 pKa = 7.85RR52 pKa = 11.84TKK54 pKa = 10.23QAMRR58 pKa = 11.84VARR61 pKa = 11.84RR62 pKa = 11.84FGRR65 pKa = 11.84FF66 pKa = 2.87

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3259

0

3259

1031760

26

2364

316.6

34.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.779 ± 0.067

0.867 ± 0.013

6.4 ± 0.042

5.835 ± 0.044

3.52 ± 0.023

9.227 ± 0.04

1.962 ± 0.022

4.868 ± 0.029

2.215 ± 0.033

10.035 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.565 ± 0.019

2.072 ± 0.021

5.54 ± 0.036

2.623 ± 0.022

8.077 ± 0.047

4.552 ± 0.025

5.245 ± 0.027

7.22 ± 0.037

1.443 ± 0.02

1.952 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski