Streptococcus phage Javan252

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6AZZ8|A0A4D6AZZ8_9CAUD DNA replication protein OS=Streptococcus phage Javan252 OX=2548072 GN=Javan252_0041 PE=4 SV=1
MM1 pKa = 7.3ITTSDD6 pKa = 3.97YY7 pKa = 11.61EE8 pKa = 4.16DD9 pKa = 4.98LSDD12 pKa = 5.64LFCLLKK18 pKa = 10.6ADD20 pKa = 4.82ADD22 pKa = 3.64TGYY25 pKa = 10.83YY26 pKa = 9.49SEE28 pKa = 5.61LMQSTGLFDD37 pKa = 4.23KK38 pKa = 11.22NGVEE42 pKa = 4.0IFEE45 pKa = 4.35GDD47 pKa = 3.54IIVAMSQGVKK57 pKa = 10.51AIGEE61 pKa = 4.19VKK63 pKa = 10.35RR64 pKa = 11.84RR65 pKa = 11.84IDD67 pKa = 4.55GYY69 pKa = 9.45WLMYY73 pKa = 9.55PALQHH78 pKa = 6.27GEE80 pKa = 3.58LWHH83 pKa = 7.19IVEE86 pKa = 4.65NVDD89 pKa = 3.19TGEE92 pKa = 4.12TGVEE96 pKa = 4.17VVGNIYY102 pKa = 10.2EE103 pKa = 4.16NPEE106 pKa = 3.84LVEE109 pKa = 4.27VEE111 pKa = 4.3NEE113 pKa = 3.74

Molecular weight: 12.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6AWL8|A0A4D6AWL8_9CAUD Uncharacterized protein OS=Streptococcus phage Javan252 OX=2548072 GN=Javan252_0003 PE=4 SV=1
MM1 pKa = 7.54RR2 pKa = 11.84PKK4 pKa = 10.2RR5 pKa = 11.84YY6 pKa = 8.98PYY8 pKa = 10.35SGQKK12 pKa = 9.8KK13 pKa = 8.81KK14 pKa = 10.1QPRR17 pKa = 11.84DD18 pKa = 3.26EE19 pKa = 4.2IAEE22 pKa = 3.88LRR24 pKa = 11.84YY25 pKa = 9.64EE26 pKa = 4.37LNVLKK31 pKa = 10.29TNLSTLAHH39 pKa = 5.75QLFKK43 pKa = 11.39NN44 pKa = 3.77

Molecular weight: 5.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

11148

38

1582

206.4

23.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.086 ± 0.585

0.449 ± 0.126

6.539 ± 0.289

6.916 ± 0.477

4.055 ± 0.182

6.055 ± 0.455

1.193 ± 0.142

6.889 ± 0.328

8.701 ± 0.39

8.226 ± 0.368

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.512 ± 0.302

6.136 ± 0.31

2.655 ± 0.211

4.36 ± 0.213

3.732 ± 0.292

6.548 ± 0.443

6.611 ± 0.473

6.351 ± 0.237

1.229 ± 0.12

3.759 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski