Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium; Bifidobacterium adolescentis

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1629 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A1A333|A1A333_BIFAA Probable NADH-dependent flavin oxidoreductase YqjM OS=Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a) OX=367928 GN=baiC PE=4 SV=1
MM1 pKa = 7.12MVDD4 pKa = 3.85FDD6 pKa = 4.24AAVAAVQDD14 pKa = 4.38PNADD18 pKa = 3.33PMFLAKK24 pKa = 9.76IAYY27 pKa = 7.22EE28 pKa = 4.01NPEE31 pKa = 3.69FGANVVANPRR41 pKa = 11.84AYY43 pKa = 9.5PGLKK47 pKa = 9.26RR48 pKa = 11.84WVAEE52 pKa = 4.31FGDD55 pKa = 3.76EE56 pKa = 4.55RR57 pKa = 11.84ARR59 pKa = 11.84QQLVAMGWPVPQNGVQPHH77 pKa = 7.16LIAEE81 pKa = 4.42PTAQPMAEE89 pKa = 3.87PVAEE93 pKa = 4.0QVQPEE98 pKa = 4.43QQYY101 pKa = 9.3QPAVAEE107 pKa = 4.14QFQQEE112 pKa = 4.12QQYY115 pKa = 9.93QPEE118 pKa = 4.26QPVNQQYY125 pKa = 10.05GQDD128 pKa = 3.31AAYY131 pKa = 9.86AASNEE136 pKa = 3.78ASTEE140 pKa = 4.03YY141 pKa = 10.63VDD143 pKa = 5.55PYY145 pKa = 10.54MNPADD150 pKa = 3.93LSEE153 pKa = 4.2VADD156 pKa = 3.95YY157 pKa = 11.29SSSQPQNKK165 pKa = 9.34PEE167 pKa = 3.84QQYY170 pKa = 11.45AEE172 pKa = 4.08QQYY175 pKa = 11.04AAAQEE180 pKa = 4.43SPADD184 pKa = 3.51AGFTAEE190 pKa = 4.92LAMTTTDD197 pKa = 3.08QMLMARR203 pKa = 11.84IASEE207 pKa = 3.75ASEE210 pKa = 4.78LRR212 pKa = 11.84PCLARR217 pKa = 11.84NPYY220 pKa = 9.88IYY222 pKa = 10.08PEE224 pKa = 4.07LLAWLGQLNDD234 pKa = 3.59SAINAAIRR242 pKa = 11.84LRR244 pKa = 11.84QQQ246 pKa = 2.65

Molecular weight: 27.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0ZZB8|A0ZZB8_BIFAA Uncharacterized protein OS=Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a) OX=367928 GN=ybfG PE=4 SV=1
MM1 pKa = 8.13RR2 pKa = 11.84SRR4 pKa = 11.84ALAWLKK10 pKa = 9.96TPGALIMKK18 pKa = 8.23RR19 pKa = 11.84TFQPNNRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84HH29 pKa = 4.82MKK31 pKa = 9.62HH32 pKa = 6.11GFRR35 pKa = 11.84QRR37 pKa = 11.84MRR39 pKa = 11.84TRR41 pKa = 11.84AGRR44 pKa = 11.84ALINRR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84AKK53 pKa = 10.07GRR55 pKa = 11.84KK56 pKa = 8.81SLAAA60 pKa = 4.22

Molecular weight: 7.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1629

0

1629

599943

37

3111

368.3

40.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.669 ± 0.073

1.034 ± 0.021

6.536 ± 0.054

5.784 ± 0.063

3.493 ± 0.032

7.933 ± 0.046

2.249 ± 0.032

5.387 ± 0.049

4.33 ± 0.05

8.694 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.73 ± 0.031

3.479 ± 0.041

4.549 ± 0.034

3.492 ± 0.043

5.889 ± 0.071

6.057 ± 0.05

5.848 ± 0.041

7.781 ± 0.051

1.346 ± 0.027

2.718 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski