Tepidicaulis marinus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Parvibaculaceae; Tepidicaulis

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3398 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A081BDI1|A0A081BDI1_9RHIZ Lactoylglutathione lyase OS=Tepidicaulis marinus OX=1333998 GN=M2A_2598 PE=4 SV=1
FF1 pKa = 7.57PPAGNYY7 pKa = 7.55TISFNGANLTITPAALSVTANALSKK32 pKa = 11.03VYY34 pKa = 10.5GAVDD38 pKa = 3.22PALTFTHH45 pKa = 6.51SGLTGGDD52 pKa = 3.51DD53 pKa = 3.44ASIFTGALVRR63 pKa = 11.84AAGEE67 pKa = 4.31TVAGGPYY74 pKa = 10.33AITQGSLAATSNYY87 pKa = 9.14ILSFNGANFTITPAALSVTADD108 pKa = 3.88ALSKK112 pKa = 10.42IYY114 pKa = 10.95GDD116 pKa = 4.4ADD118 pKa = 3.53PALTFGFTGLTNGDD132 pKa = 3.88DD133 pKa = 3.51ASVFTGALSRR143 pKa = 11.84AAGEE147 pKa = 4.36TVAGGPYY154 pKa = 10.33AITQGTLSAGSNYY167 pKa = 9.77TISFNGANLVITPKK181 pKa = 10.16VLSVSVSADD190 pKa = 3.05DD191 pKa = 3.61KK192 pKa = 11.76VYY194 pKa = 11.05DD195 pKa = 3.78GTVAATGTVGGLGGIVGGDD214 pKa = 3.62DD215 pKa = 3.44VTASGSGSFAFNDD228 pKa = 3.64ANVGAGKK235 pKa = 10.17AVNVTGLTLSGADD248 pKa = 3.16AGNYY252 pKa = 7.14TLSPNAATSASITPAALSVSADD274 pKa = 3.74ALSKK278 pKa = 9.94TYY280 pKa = 11.2GEE282 pKa = 4.84ADD284 pKa = 3.23PSFTFNFAGFVGGDD298 pKa = 3.34NASLFTGALARR309 pKa = 11.84AAGEE313 pKa = 4.28TVAGGPYY320 pKa = 10.33AIGQGTLSAGGNYY333 pKa = 8.9TINFTGADD341 pKa = 3.73FTITPAALTITANDD355 pKa = 3.66AQKK358 pKa = 11.3DD359 pKa = 3.8EE360 pKa = 4.63GDD362 pKa = 3.65TLLLSGFSVAGLVAGDD378 pKa = 4.05SVSGVSLTSPGTVASAAPGTYY399 pKa = 10.37VITASNAAGTGLSNYY414 pKa = 10.02AITYY418 pKa = 10.04VNGQLTVIEE427 pKa = 4.49MPLVPSNPLPGTPPLPGDD445 pKa = 3.19ITLTSFQPGTLQNAWRR461 pKa = 11.84PEE463 pKa = 3.81DD464 pKa = 3.6QPIGKK469 pKa = 10.1GEE471 pKa = 4.0GAGGFWTLPILLQLGGFGGGADD493 pKa = 4.3DD494 pKa = 4.91GGLVTGSIGQGDD506 pKa = 4.44GQSGGPNDD514 pKa = 4.15GSDD517 pKa = 3.47EE518 pKa = 4.09EE519 pKa = 4.37RR520 pKa = 11.84WNLFTGQAGRR530 pKa = 11.84FGSYY534 pKa = 9.15TNIGVQDD541 pKa = 4.25LRR543 pKa = 11.84PLPVTGTFSATTEE556 pKa = 3.88

Molecular weight: 54.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A081B819|A0A081B819_9RHIZ Auxin efflux carrier OS=Tepidicaulis marinus OX=1333998 GN=M2A_0686 PE=4 SV=1
MM1 pKa = 7.33ATTAKK6 pKa = 10.4RR7 pKa = 11.84KK8 pKa = 5.61TTAKK12 pKa = 10.3KK13 pKa = 8.81KK14 pKa = 8.28TAAKK18 pKa = 9.86KK19 pKa = 9.02KK20 pKa = 9.64AAVKK24 pKa = 9.28RR25 pKa = 11.84TAKK28 pKa = 8.91KK29 pKa = 7.81TAPKK33 pKa = 9.89RR34 pKa = 11.84KK35 pKa = 7.33TVAKK39 pKa = 10.09RR40 pKa = 11.84KK41 pKa = 7.12TAAKK45 pKa = 9.97KK46 pKa = 8.82KK47 pKa = 7.93TAAKK51 pKa = 8.46KK52 pKa = 7.84TAAKK56 pKa = 10.18RR57 pKa = 11.84KK58 pKa = 6.81TAAKK62 pKa = 9.85KK63 pKa = 9.72KK64 pKa = 9.63AAPKK68 pKa = 10.25RR69 pKa = 11.84KK70 pKa = 7.32TAAKK74 pKa = 9.92KK75 pKa = 8.86KK76 pKa = 8.11TAAKK80 pKa = 9.93KK81 pKa = 9.68KK82 pKa = 9.67AAPKK86 pKa = 10.25RR87 pKa = 11.84KK88 pKa = 7.32TAAKK92 pKa = 9.92KK93 pKa = 8.86KK94 pKa = 8.03TAAKK98 pKa = 10.08KK99 pKa = 8.86KK100 pKa = 8.04TAAKK104 pKa = 9.93KK105 pKa = 9.68KK106 pKa = 9.67AAPKK110 pKa = 10.25RR111 pKa = 11.84KK112 pKa = 7.38TAAKK116 pKa = 9.82KK117 pKa = 9.72KK118 pKa = 9.77AAPKK122 pKa = 10.06RR123 pKa = 11.84KK124 pKa = 9.19AAAKK128 pKa = 9.98KK129 pKa = 9.66KK130 pKa = 9.67AAPKK134 pKa = 10.12RR135 pKa = 11.84KK136 pKa = 9.1VARR139 pKa = 11.84KK140 pKa = 9.65KK141 pKa = 10.43KK142 pKa = 10.08

Molecular weight: 15.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3398

0

3398

1037151

40

1577

305.2

33.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.332 ± 0.055

0.799 ± 0.013

5.417 ± 0.033

7.118 ± 0.041

3.811 ± 0.029

8.708 ± 0.042

2.043 ± 0.021

5.081 ± 0.03

4.151 ± 0.033

10.165 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.555 ± 0.019

2.623 ± 0.025

5.058 ± 0.032

3.051 ± 0.022

6.551 ± 0.039

5.168 ± 0.029

4.974 ± 0.03

6.713 ± 0.037

1.295 ± 0.018

2.385 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski