Amino acid dipepetide frequency for Streptomyces sp. NRRL B-3648

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.989AlaAla: 21.989 ± 0.159	1.147AlaCys: 1.147 ± 0.022	8.404AlaAsp: 8.404 ± 0.071	8.85AlaGlu: 8.85 ± 0.076	3.538AlaPhe: 3.538 ± 0.044	14.36AlaGly: 14.36 ± 0.096	3.076AlaHis: 3.076 ± 0.043	3.042AlaIle: 3.042 ± 0.038	2.602AlaLys: 2.602 ± 0.046	14.945AlaLeu: 14.945 ± 0.115	2.294AlaMet: 2.294 ± 0.036	1.845AlaAsn: 1.845 ± 0.029	7.361AlaPro: 7.361 ± 0.08	3.757AlaGln: 3.757 ± 0.048	11.079AlaArg: 11.079 ± 0.081	5.503AlaSer: 5.503 ± 0.053	6.877AlaThr: 6.877 ± 0.066	12.837AlaVal: 12.837 ± 0.112	1.96AlaTrp: 1.96 ± 0.028	2.9AlaTyr: 2.9 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	1.174CysAla: 1.174 ± 0.024	0.102CysCys: 0.102 ± 0.007	0.453CysAsp: 0.453 ± 0.015	0.393CysGlu: 0.393 ± 0.014	0.221CysPhe: 0.221 ± 0.01	0.971CysGly: 0.971 ± 0.022	0.197CysHis: 0.197 ± 0.011	0.154CysIle: 0.154 ± 0.007	0.129CysLys: 0.129 ± 0.008	0.798CysLeu: 0.798 ± 0.02	0.133CysMet: 0.133 ± 0.008	0.139CysAsn: 0.139 ± 0.008	0.499CysPro: 0.499 ± 0.016	0.173CysGln: 0.173 ± 0.008	0.636CysArg: 0.636 ± 0.019	0.435CysSer: 0.435 ± 0.016	0.544CysThr: 0.544 ± 0.016	0.688CysVal: 0.688 ± 0.02	0.13CysTrp: 0.13 ± 0.008	0.162CysTyr: 0.162 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.565AspAla: 7.565 ± 0.075	0.435AspCys: 0.435 ± 0.015	3.428AspAsp: 3.428 ± 0.043	3.6AspGlu: 3.6 ± 0.045	1.616AspPhe: 1.616 ± 0.027	6.465AspGly: 6.465 ± 0.067	1.459AspHis: 1.459 ± 0.027	1.763AspIle: 1.763 ± 0.031	1.148AspLys: 1.148 ± 0.031	6.186AspLeu: 6.186 ± 0.06	0.77AspMet: 0.77 ± 0.02	0.882AspAsn: 0.882 ± 0.022	4.599AspPro: 4.599 ± 0.049	1.497AspGln: 1.497 ± 0.029	5.085AspArg: 5.085 ± 0.055	2.405AspSer: 2.405 ± 0.037	3.348AspThr: 3.348 ± 0.037	4.669AspVal: 4.669 ± 0.049	1.095AspTrp: 1.095 ± 0.024	1.11AspTyr: 1.11 ± 0.026	0.0AspXaa: 0.0 ± 0.0
Glu	7.328GluAla: 7.328 ± 0.067	0.371GluCys: 0.371 ± 0.013	2.794GluAsp: 2.794 ± 0.038	3.569GluGlu: 3.569 ± 0.052	1.414GluPhe: 1.414 ± 0.027	4.132GluGly: 4.132 ± 0.045	1.553GluHis: 1.553 ± 0.028	2.105GluIle: 2.105 ± 0.036	1.332GluLys: 1.332 ± 0.031	6.704GluLeu: 6.704 ± 0.073	0.829GluMet: 0.829 ± 0.022	0.942GluAsn: 0.942 ± 0.021	3.42GluPro: 3.42 ± 0.038	2.244GluGln: 2.244 ± 0.035	5.489GluArg: 5.489 ± 0.06	2.308GluSer: 2.308 ± 0.034	2.855GluThr: 2.855 ± 0.036	4.356GluVal: 4.356 ± 0.047	0.736GluTrp: 0.736 ± 0.018	1.087GluTyr: 1.087 ± 0.023	0.0GluXaa: 0.0 ± 0.0
Phe	3.718PheAla: 3.718 ± 0.045	0.273PheCys: 0.273 ± 0.01	1.905PheAsp: 1.905 ± 0.033	1.384PheGlu: 1.384 ± 0.025	0.873PhePhe: 0.873 ± 0.021	2.955PheGly: 2.955 ± 0.039	0.633PheHis: 0.633 ± 0.018	0.635PheIle: 0.635 ± 0.016	0.503PheLys: 0.503 ± 0.018	2.649PheLeu: 2.649 ± 0.039	0.373PheMet: 0.373 ± 0.013	0.514PheAsn: 0.514 ± 0.017	1.447PhePro: 1.447 ± 0.027	0.68PheGln: 0.68 ± 0.017	1.915PheArg: 1.915 ± 0.029	1.407PheSer: 1.407 ± 0.027	2.016PheThr: 2.016 ± 0.032	2.225PheVal: 2.225 ± 0.034	0.416PheTrp: 0.416 ± 0.014	0.563PheTyr: 0.563 ± 0.015	0.0PheXaa: 0.0 ± 0.0
Gly	11.396GlyAla: 11.396 ± 0.092	0.853GlyCys: 0.853 ± 0.02	5.242GlyAsp: 5.242 ± 0.053	5.05GlyGlu: 5.05 ± 0.048	2.898GlyPhe: 2.898 ± 0.038	8.985GlyGly: 8.985 ± 0.092	2.577GlyHis: 2.577 ± 0.037	3.333GlyIle: 3.333 ± 0.041	2.342GlyLys: 2.342 ± 0.044	9.596GlyLeu: 9.596 ± 0.07	1.972GlyMet: 1.972 ± 0.032	1.703GlyAsn: 1.703 ± 0.034	5.452GlyPro: 5.452 ± 0.065	2.65GlyGln: 2.65 ± 0.037	8.491GlyArg: 8.491 ± 0.069	5.242GlySer: 5.242 ± 0.057	6.989GlyThr: 6.989 ± 0.072	7.722GlyVal: 7.722 ± 0.063	1.744GlyTrp: 1.744 ± 0.033	2.355GlyTyr: 2.355 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	2.806HisAla: 2.806 ± 0.037	0.219HisCys: 0.219 ± 0.011	1.343HisAsp: 1.343 ± 0.023	1.256HisGlu: 1.256 ± 0.022	0.644HisPhe: 0.644 ± 0.017	2.631HisGly: 2.631 ± 0.038	0.732HisHis: 0.732 ± 0.022	0.648HisIle: 0.648 ± 0.018	0.354HisLys: 0.354 ± 0.015	2.565HisLeu: 2.565 ± 0.034	0.342HisMet: 0.342 ± 0.012	0.354HisAsn: 0.354 ± 0.013	1.941HisPro: 1.941 ± 0.034	0.648HisGln: 0.648 ± 0.018	2.281HisArg: 2.281 ± 0.034	1.022HisSer: 1.022 ± 0.021	1.443HisThr: 1.443 ± 0.025	1.743HisVal: 1.743 ± 0.031	0.397HisTrp: 0.397 ± 0.014	0.515HisTyr: 0.515 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	4.414IleAla: 4.414 ± 0.048	0.258IleCys: 0.258 ± 0.012	2.053IleAsp: 2.053 ± 0.037	1.805IleGlu: 1.805 ± 0.034	0.613IlePhe: 0.613 ± 0.02	3.34IleGly: 3.34 ± 0.038	0.578IleHis: 0.578 ± 0.016	0.762IleIle: 0.762 ± 0.019	0.633IleLys: 0.633 ± 0.018	2.249IleLeu: 2.249 ± 0.031	0.39IleMet: 0.39 ± 0.013	0.61IleAsn: 0.61 ± 0.018	1.608IlePro: 1.608 ± 0.028	0.658IleGln: 0.658 ± 0.018	2.2IleArg: 2.2 ± 0.035	1.484IleSer: 1.484 ± 0.026	2.086IleThr: 2.086 ± 0.031	2.539IleVal: 2.539 ± 0.033	0.34IleTrp: 0.34 ± 0.013	0.495IleTyr: 0.495 ± 0.016	0.0IleXaa: 0.0 ± 0.0
Lys	2.81LysAla: 2.81 ± 0.05	0.121LysCys: 0.121 ± 0.008	1.298LysAsp: 1.298 ± 0.03	1.103LysGlu: 1.103 ± 0.025	0.432LysPhe: 0.432 ± 0.016	1.802LysGly: 1.802 ± 0.035	0.396LysHis: 0.396 ± 0.014	0.782LysIle: 0.782 ± 0.019	0.817LysLys: 0.817 ± 0.029	1.86LysLeu: 1.86 ± 0.035	0.353LysMet: 0.353 ± 0.013	0.469LysAsn: 0.469 ± 0.018	1.246LysPro: 1.246 ± 0.027	0.634LysGln: 0.634 ± 0.018	1.361LysArg: 1.361 ± 0.025	1.07LysSer: 1.07 ± 0.026	1.188LysThr: 1.188 ± 0.032	1.827LysVal: 1.827 ± 0.038	0.271LysTrp: 0.271 ± 0.012	0.435LysTyr: 0.435 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	15.745LeuAla: 15.745 ± 0.114	0.868LeuCys: 0.868 ± 0.022	6.742LeuAsp: 6.742 ± 0.055	4.697LeuGlu: 4.697 ± 0.05	2.616LeuPhe: 2.616 ± 0.035	9.424LeuGly: 9.424 ± 0.071	2.427LeuHis: 2.427 ± 0.037	3.05LeuIle: 3.05 ± 0.038	2.026LeuLys: 2.026 ± 0.037	11.629LeuLeu: 11.629 ± 0.104	1.591LeuMet: 1.591 ± 0.033	1.561LeuAsn: 1.561 ± 0.025	6.737LeuPro: 6.737 ± 0.06	2.156LeuGln: 2.156 ± 0.034	8.921LeuArg: 8.921 ± 0.073	5.076LeuSer: 5.076 ± 0.049	7.075LeuThr: 7.075 ± 0.066	8.966LeuVal: 8.966 ± 0.079	1.356LeuTrp: 1.356 ± 0.03	1.932LeuTyr: 1.932 ± 0.032	0.0LeuXaa: 0.0 ± 0.0
Met	2.205MetAla: 2.205 ± 0.033	0.147MetCys: 0.147 ± 0.007	0.833MetAsp: 0.833 ± 0.02	0.724MetGlu: 0.724 ± 0.018	0.441MetPhe: 0.441 ± 0.015	1.274MetGly: 1.274 ± 0.029	0.37MetHis: 0.37 ± 0.013	0.587MetIle: 0.587 ± 0.018	0.366MetLys: 0.366 ± 0.013	1.589MetLeu: 1.589 ± 0.026	0.263MetMet: 0.263 ± 0.011	0.373MetAsn: 0.373 ± 0.013	1.068MetPro: 1.068 ± 0.021	0.427MetGln: 0.427 ± 0.016	1.375MetArg: 1.375 ± 0.026	1.158MetSer: 1.158 ± 0.025	1.416MetThr: 1.416 ± 0.028	1.263MetVal: 1.263 ± 0.025	0.215MetTrp: 0.215 ± 0.01	0.317MetTyr: 0.317 ± 0.013	0.0MetXaa: 0.0 ± 0.0
Asn	2.056AsnAla: 2.056 ± 0.033	0.156AsnCys: 0.156 ± 0.008	0.875AsnAsp: 0.875 ± 0.02	0.742AsnGlu: 0.742 ± 0.022	0.455AsnPhe: 0.455 ± 0.015	1.823AsnGly: 1.823 ± 0.039	0.393AsnHis: 0.393 ± 0.014	0.595AsnIle: 0.595 ± 0.018	0.372AsnLys: 0.372 ± 0.016	1.543AsnLeu: 1.543 ± 0.03	0.285AsnMet: 0.285 ± 0.013	0.38AsnAsn: 0.38 ± 0.015	1.276AsnPro: 1.276 ± 0.023	0.479AsnGln: 0.479 ± 0.017	1.196AsnArg: 1.196 ± 0.021	0.881AsnSer: 0.881 ± 0.024	1.059AsnThr: 1.059 ± 0.028	1.372AsnVal: 1.372 ± 0.03	0.279AsnTrp: 0.279 ± 0.011	0.386AsnTyr: 0.386 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	9.556ProAla: 9.556 ± 0.095	0.35ProCys: 0.35 ± 0.013	4.606ProAsp: 4.606 ± 0.048	4.344ProGlu: 4.344 ± 0.048	1.515ProPhe: 1.515 ± 0.026	7.177ProGly: 7.177 ± 0.074	1.474ProHis: 1.474 ± 0.028	1.086ProIle: 1.086 ± 0.024	1.062ProLys: 1.062 ± 0.024	5.559ProLeu: 5.559 ± 0.059	0.942ProMet: 0.942 ± 0.022	0.817ProAsn: 0.817 ± 0.021	3.589ProPro: 3.589 ± 0.052	1.752ProGln: 1.752 ± 0.035	4.214ProArg: 4.214 ± 0.051	2.918ProSer: 2.918 ± 0.037	2.871ProThr: 2.871 ± 0.039	5.87ProVal: 5.87 ± 0.066	0.9ProTrp: 0.9 ± 0.023	1.506ProTyr: 1.506 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	3.834GlnAla: 3.834 ± 0.044	0.165GlnCys: 0.165 ± 0.01	1.41GlnAsp: 1.41 ± 0.026	1.447GlnGlu: 1.447 ± 0.03	0.649GlnPhe: 0.649 ± 0.018	2.371GlnGly: 2.371 ± 0.035	0.646GlnHis: 0.646 ± 0.015	1.006GlnIle: 1.006 ± 0.025	0.576GlnLys: 0.576 ± 0.018	2.956GlnLeu: 2.956 ± 0.041	0.442GlnMet: 0.442 ± 0.015	0.493GlnAsn: 0.493 ± 0.017	1.753GlnPro: 1.753 ± 0.037	1.225GlnGln: 1.225 ± 0.028	2.382GlnArg: 2.382 ± 0.035	1.184GlnSer: 1.184 ± 0.025	1.312GlnThr: 1.312 ± 0.029	2.378GlnVal: 2.378 ± 0.036	0.477GlnTrp: 0.477 ± 0.014	0.604GlnTyr: 0.604 ± 0.019	0.0GlnXaa: 0.0 ± 0.0
Arg	10.414ArgAla: 10.414 ± 0.076	0.654ArgCys: 0.654 ± 0.018	4.277ArgAsp: 4.277 ± 0.05	4.855ArgGlu: 4.855 ± 0.059	2.429ArgPhe: 2.429 ± 0.035	6.007ArgGly: 6.007 ± 0.06	2.317ArgHis: 2.317 ± 0.034	3.2ArgIle: 3.2 ± 0.036	1.567ArgLys: 1.567 ± 0.027	9.532ArgLeu: 9.532 ± 0.083	1.734ArgMet: 1.734 ± 0.03	1.338ArgAsn: 1.338 ± 0.027	5.364ArgPro: 5.364 ± 0.056	2.399ArgGln: 2.399 ± 0.033	8.047ArgArg: 8.047 ± 0.091	3.994ArgSer: 3.994 ± 0.042	5.669ArgThr: 5.669 ± 0.063	6.057ArgVal: 6.057 ± 0.056	1.398ArgTrp: 1.398 ± 0.028	1.927ArgTyr: 1.927 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	6.548SerAla: 6.548 ± 0.058	0.415SerCys: 0.415 ± 0.013	2.459SerAsp: 2.459 ± 0.033	2.111SerGlu: 2.111 ± 0.033	1.454SerPhe: 1.454 ± 0.027	5.81SerGly: 5.81 ± 0.061	0.981SerHis: 0.981 ± 0.023	1.192SerIle: 1.192 ± 0.023	0.904SerLys: 0.904 ± 0.026	4.719SerLeu: 4.719 ± 0.053	0.991SerMet: 0.991 ± 0.02	0.797SerAsn: 0.797 ± 0.023	3.038SerPro: 3.038 ± 0.041	1.152SerGln: 1.152 ± 0.026	3.565SerArg: 3.565 ± 0.041	2.544SerSer: 2.544 ± 0.041	2.817SerThr: 2.817 ± 0.042	4.146SerVal: 4.146 ± 0.042	0.866SerTrp: 0.866 ± 0.02	1.258SerTyr: 1.258 ± 0.026	0.0SerXaa: 0.0 ± 0.0
Thr	9.419ThrAla: 9.419 ± 0.074	0.443ThrCys: 0.443 ± 0.015	3.591ThrAsp: 3.591 ± 0.041	3.202ThrGlu: 3.202 ± 0.038	1.613ThrPhe: 1.613 ± 0.029	7.214ThrGly: 7.214 ± 0.072	1.2ThrHis: 1.2 ± 0.025	1.525ThrIle: 1.525 ± 0.03	1.08ThrLys: 1.08 ± 0.026	5.804ThrLeu: 5.804 ± 0.054	0.866ThrMet: 0.866 ± 0.021	0.929ThrAsn: 0.929 ± 0.025	4.164ThrPro: 4.164 ± 0.051	1.27ThrGln: 1.27 ± 0.025	4.042ThrArg: 4.042 ± 0.042	2.969ThrSer: 2.969 ± 0.042	3.711ThrThr: 3.711 ± 0.061	6.233ThrVal: 6.233 ± 0.051	0.887ThrTrp: 0.887 ± 0.019	1.36ThrTyr: 1.36 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	10.779ValAla: 10.779 ± 0.09	0.815ValCys: 0.815 ± 0.02	4.902ValAsp: 4.902 ± 0.051	4.502ValGlu: 4.502 ± 0.049	2.506ValPhe: 2.506 ± 0.037	6.35ValGly: 6.35 ± 0.064	2.057ValHis: 2.057 ± 0.028	2.668ValIle: 2.668 ± 0.038	1.705ValLys: 1.705 ± 0.03	9.761ValLeu: 9.761 ± 0.09	1.331ValMet: 1.331 ± 0.026	1.657ValAsn: 1.657 ± 0.028	5.636ValPro: 5.636 ± 0.059	2.212ValGln: 2.212 ± 0.036	7.646ValArg: 7.646 ± 0.057	4.252ValSer: 4.252 ± 0.044	5.927ValThr: 5.927 ± 0.048	8.028ValVal: 8.028 ± 0.067	1.197ValTrp: 1.197 ± 0.026	1.664ValTyr: 1.664 ± 0.027	0.0ValXaa: 0.0 ± 0.0
Trp	1.711TrpAla: 1.711 ± 0.027	0.151TrpCys: 0.151 ± 0.008	0.885TrpAsp: 0.885 ± 0.024	0.723TrpGlu: 0.723 ± 0.02	0.511TrpPhe: 0.511 ± 0.016	1.07TrpGly: 1.07 ± 0.022	0.39TrpHis: 0.39 ± 0.011	0.526TrpIle: 0.526 ± 0.014	0.358TrpLys: 0.358 ± 0.013	1.804TrpLeu: 1.804 ± 0.035	0.26TrpMet: 0.26 ± 0.01	0.405TrpAsn: 0.405 ± 0.015	0.803TrpPro: 0.803 ± 0.02	0.661TrpGln: 0.661 ± 0.018	1.382TrpArg: 1.382 ± 0.028	0.97TrpSer: 0.97 ± 0.024	1.07TrpThr: 1.07 ± 0.025	0.973TrpVal: 0.973 ± 0.021	0.338TrpTrp: 0.338 ± 0.013	0.376TrpTyr: 0.376 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.897TyrAla: 2.897 ± 0.037	0.184TyrCys: 0.184 ± 0.008	1.626TyrAsp: 1.626 ± 0.038	1.208TyrGlu: 1.208 ± 0.022	0.648TyrPhe: 0.648 ± 0.018	2.432TyrGly: 2.432 ± 0.033	0.428TyrHis: 0.428 ± 0.014	0.458TyrIle: 0.458 ± 0.014	0.399TyrLys: 0.399 ± 0.015	2.147TyrLeu: 2.147 ± 0.036	0.252TyrMet: 0.252 ± 0.01	0.394TyrAsn: 0.394 ± 0.015	1.11TyrPro: 1.11 ± 0.023	0.637TyrGln: 0.637 ± 0.017	1.873TyrArg: 1.873 ± 0.032	0.942TyrSer: 0.942 ± 0.022	1.249TyrThr: 1.249 ± 0.026	1.703TyrVal: 1.703 ± 0.031	0.366TyrTrp: 0.366 ± 0.014	0.457TyrTyr: 0.457 ± 0.017	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7028 proteins (2248530 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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