Lactococcus phage 63301

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B1IMS7|A0A1B1IMS7_9CAUD Major head protein OS=Lactococcus phage 63301 OX=1871687 GN=DS63301_32 PE=4 SV=1
MM1 pKa = 8.09RR2 pKa = 11.84YY3 pKa = 9.93FNTLTKK9 pKa = 10.14ATIDD13 pKa = 3.43TDD15 pKa = 4.12FKK17 pKa = 11.04ISGGDD22 pKa = 2.93WVLEE26 pKa = 4.2NEE28 pKa = 4.4SKK30 pKa = 10.81EE31 pKa = 4.08AVVDD35 pKa = 3.84IQANDD40 pKa = 3.7ADD42 pKa = 4.33SKK44 pKa = 10.94KK45 pKa = 10.7AEE47 pKa = 3.96QEE49 pKa = 4.2QVVEE53 pKa = 4.24EE54 pKa = 4.62SNVDD58 pKa = 3.79GNYY61 pKa = 10.98DD62 pKa = 4.32WITKK66 pKa = 8.69DD67 pKa = 4.44QIMQEE72 pKa = 3.78LDD74 pKa = 3.2AFGIRR79 pKa = 11.84YY80 pKa = 8.45DD81 pKa = 3.38KK82 pKa = 10.88RR83 pKa = 11.84ANKK86 pKa = 8.98QVLYY90 pKa = 10.72DD91 pKa = 4.1LMMEE95 pKa = 4.15QGKK98 pKa = 8.58EE99 pKa = 3.75

Molecular weight: 11.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B1IMQ6|A0A1B1IMQ6_9CAUD Uncharacterized protein OS=Lactococcus phage 63301 OX=1871687 GN=DS63301_13 PE=4 SV=1
MM1 pKa = 7.62HH2 pKa = 7.57YY3 pKa = 9.71IPKK6 pKa = 10.05YY7 pKa = 9.07SRR9 pKa = 11.84EE10 pKa = 4.05RR11 pKa = 11.84QKK13 pKa = 10.94KK14 pKa = 8.58RR15 pKa = 11.84RR16 pKa = 11.84NNDD19 pKa = 3.27VIRR22 pKa = 11.84VTTSPFLTEE31 pKa = 3.67VHH33 pKa = 6.7SIADD37 pKa = 3.34ATVNIVRR44 pKa = 11.84NEE46 pKa = 3.75IKK48 pKa = 10.3KK49 pKa = 10.67ANYY52 pKa = 9.71QIGKK56 pKa = 9.61

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

9700

21

843

202.1

22.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.505 ± 0.591

0.691 ± 0.164

6.144 ± 0.324

6.784 ± 0.591

4.041 ± 0.235

6.454 ± 0.532

1.227 ± 0.15

7.794 ± 0.337

8.856 ± 0.594

8.144 ± 0.26

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.289 ± 0.141

5.948 ± 0.289

3.155 ± 0.192

3.959 ± 0.229

3.814 ± 0.382

7.206 ± 0.477

6.258 ± 0.416

5.773 ± 0.287

1.278 ± 0.106

3.68 ± 0.293
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski