Aspergillus sclerotioniger CBS 115572
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 12304 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A317WSN0|A0A317WSN0_9EURO DNA mismatch repair protein Msh2 OS=Aspergillus sclerotioniger CBS 115572 OX=1450535 GN=BO94DRAFT_534891 PE=3 SV=1
MM1 pKa = 6.5 VTSSSLIAVGLTLWASLVSATPADD25 pKa = 3.91 PRR27 pKa = 11.84 VTAAPKK33 pKa = 10.88 LEE35 pKa = 4.8 DD36 pKa = 3.6 LDD38 pKa = 4.91 KK39 pKa = 11.08 RR40 pKa = 11.84 ATSCTFSGSEE50 pKa = 4.27 GASSASKK57 pKa = 10.82 SKK59 pKa = 9.53 TSCSTIVLSDD69 pKa = 3.44 VAVPSGTTLDD79 pKa = 3.71 LTDD82 pKa = 4.74 LNEE85 pKa = 4.24 GTHH88 pKa = 6.42 VIFEE92 pKa = 4.49 GEE94 pKa = 4.12 TTFGYY99 pKa = 10.09 EE100 pKa = 3.78 EE101 pKa = 4.23 WDD103 pKa = 3.84 GPLVSVSGTDD113 pKa = 3.23 ITVTGADD120 pKa = 3.38 GAYY123 pKa = 10.71 LNGDD127 pKa = 3.92 GSRR130 pKa = 11.84 WWDD133 pKa = 3.38 DD134 pKa = 3.26 EE135 pKa = 4.13 GSNGGKK141 pKa = 8.24 TKK143 pKa = 10.59 PKK145 pKa = 10.15 FFYY148 pKa = 9.6 AHH150 pKa = 6.08 NMIDD154 pKa = 3.63 STISGIYY161 pKa = 8.6 IQNSPVQVFSIDD173 pKa = 3.43 DD174 pKa = 3.53 SSYY177 pKa = 10.58 LTLEE181 pKa = 5.51 DD182 pKa = 3.4 ITIDD186 pKa = 5.33 DD187 pKa = 4.51 SDD189 pKa = 5.3 GDD191 pKa = 4.01 DD192 pKa = 3.86 AGAANTDD199 pKa = 3.24 GFDD202 pKa = 3.65 IGSSTYY208 pKa = 9.33 ITITGANVYY217 pKa = 10.14 NQDD220 pKa = 3.1 DD221 pKa = 4.24 CVAVNSGEE229 pKa = 4.07 NIYY232 pKa = 10.7 FSGGVCSGGHH242 pKa = 5.7 GLSIGSVGGRR252 pKa = 11.84 DD253 pKa = 3.68 DD254 pKa = 3.79 NTVKK258 pKa = 10.8 NVTFYY263 pKa = 11.21 NSEE266 pKa = 3.87 IKK268 pKa = 10.57 SSQNGVRR275 pKa = 11.84 IKK277 pKa = 9.88 TIYY280 pKa = 10.53 GDD282 pKa = 3.58 TGSVSDD288 pKa = 3.59 ITYY291 pKa = 10.76 EE292 pKa = 4.34 KK293 pKa = 9.38 ITLSEE298 pKa = 3.69 ITKK301 pKa = 9.75 YY302 pKa = 10.99 GIVVEE307 pKa = 4.11 QNYY310 pKa = 10.92 DD311 pKa = 3.58 DD312 pKa = 4.16 TSKK315 pKa = 11.27 SPTDD319 pKa = 4.34 GITIEE324 pKa = 4.51 NFILDD329 pKa = 4.06 GVEE332 pKa = 4.18 GTVEE336 pKa = 3.89 SSGTNIYY343 pKa = 9.42 IVCGSDD349 pKa = 4.0 SCTDD353 pKa = 3.53 WTWTTVDD360 pKa = 2.81 ITGGKK365 pKa = 7.95 TSSDD369 pKa = 4.06 CEE371 pKa = 4.14 NVPDD375 pKa = 6.29 DD376 pKa = 4.16 ISCC379 pKa = 3.93
Molecular weight: 39.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.691
IPC2_protein 3.783
IPC_protein 3.821
Toseland 3.592
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.643
Grimsley 3.49
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.202
Thurlkill 3.643
EMBOSS 3.77
Sillero 3.948
Patrickios 1.049
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.821
Protein with the highest isoelectric point:
>tr|A0A317WJ69|A0A317WJ69_9EURO DUF1212-domain-containing protein OS=Aspergillus sclerotioniger CBS 115572 OX=1450535 GN=BO94DRAFT_467054 PE=3 SV=1
MM1 pKa = 7.88 PSHH4 pKa = 6.91 KK5 pKa = 10.39 SFRR8 pKa = 11.84 TKK10 pKa = 10.45 QKK12 pKa = 9.54 LAKK15 pKa = 9.74 AQRR18 pKa = 11.84 QNRR21 pKa = 11.84 PIPQWIRR28 pKa = 11.84 LRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.89 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.14 WRR45 pKa = 11.84 KK46 pKa = 7.41 TRR48 pKa = 11.84 LGII51 pKa = 4.46
Molecular weight: 6.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
12304
0
12304
5731707
49
9565
465.8
51.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.316 ± 0.02
1.334 ± 0.01
5.591 ± 0.016
6.008 ± 0.025
3.769 ± 0.013
6.814 ± 0.018
2.497 ± 0.01
5.037 ± 0.016
4.314 ± 0.02
9.258 ± 0.024
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.2 ± 0.009
3.53 ± 0.011
6.094 ± 0.026
3.989 ± 0.016
6.159 ± 0.018
8.29 ± 0.024
6.071 ± 0.018
6.274 ± 0.019
1.536 ± 0.009
2.92 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here