Acidipila sp. 4G-K13

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Acidobacteriia; Acidobacteriales; Acidobacteriaceae; Acidipila; unclassified Acidipila

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3979 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A372IRA8|A0A372IRA8_9BACT FliM/FliN family flagellar motor switch protein OS=Acidipila sp. 4G-K13 OX=2303751 GN=D0Y96_04530 PE=4 SV=1
MM1 pKa = 7.61RR2 pKa = 11.84VDD4 pKa = 3.31SQYY7 pKa = 11.39VSNLTAALDD16 pKa = 3.66AATANQAQISQEE28 pKa = 3.66ISDD31 pKa = 3.72GRR33 pKa = 11.84RR34 pKa = 11.84INALSDD40 pKa = 3.49DD41 pKa = 3.98PVAVGSNVLLNAEE54 pKa = 4.85LGVEE58 pKa = 4.35DD59 pKa = 4.89TFSQTSDD66 pKa = 3.4AVDD69 pKa = 3.29SMLQVSDD76 pKa = 3.76SALGGVVSQLTQALSLATQANNGTLNASDD105 pKa = 3.59IQSISSQLGSILNEE119 pKa = 3.81VVALANTTYY128 pKa = 10.73LGQYY132 pKa = 8.58VFSGSQGNTPAYY144 pKa = 10.19SSTGTYY150 pKa = 10.02QGDD153 pKa = 3.94TVVSYY158 pKa = 11.59LEE160 pKa = 4.26TPSGQKK166 pKa = 9.34IQLNLPGSQIFSGSGGNVMAALNNLIADD194 pKa = 4.18YY195 pKa = 11.37SSGTPSATAAADD207 pKa = 3.72TTALNTAMTWLSAQRR222 pKa = 11.84VTLDD226 pKa = 2.96NSMTRR231 pKa = 11.84LEE233 pKa = 4.39AAQTYY238 pKa = 10.64NGTQSADD245 pKa = 3.11AEE247 pKa = 4.22AAQTNLLQTDD257 pKa = 4.15TAQAATQLSLDD268 pKa = 4.18EE269 pKa = 4.62SQQSAISQTISIIEE283 pKa = 4.06KK284 pKa = 9.96EE285 pKa = 4.31GTLFNYY291 pKa = 10.02LL292 pKa = 3.51

Molecular weight: 30.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A372IMW8|A0A372IMW8_9BACT Acyl-CoA dehydrogenase OS=Acidipila sp. 4G-K13 OX=2303751 GN=D0Y96_12980 PE=3 SV=1
MM1 pKa = 8.0PKK3 pKa = 9.02RR4 pKa = 11.84TFQPNRR10 pKa = 11.84RR11 pKa = 11.84HH12 pKa = 5.91RR13 pKa = 11.84SKK15 pKa = 8.98THH17 pKa = 5.49GFRR20 pKa = 11.84SRR22 pKa = 11.84MKK24 pKa = 8.69TKK26 pKa = 10.4SGAAVLSRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.1GRR40 pKa = 11.84HH41 pKa = 4.6RR42 pKa = 11.84VAVSAGYY49 pKa = 10.34RR50 pKa = 11.84DD51 pKa = 3.42

Molecular weight: 5.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3979

0

3979

1463424

25

2814

367.8

40.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.062 ± 0.051

0.892 ± 0.013

5.089 ± 0.029

5.565 ± 0.041

3.91 ± 0.028

8.154 ± 0.04

2.382 ± 0.021

5.037 ± 0.028

3.246 ± 0.032

9.893 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.175 ± 0.018

3.266 ± 0.041

5.421 ± 0.029

3.922 ± 0.024

6.442 ± 0.042

6.481 ± 0.044

5.9 ± 0.054

6.931 ± 0.028

1.411 ± 0.016

2.822 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski