Tortoise microvirus 34

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P8W9L8|A0A4P8W9L8_9VIRU DNA pilot protein OS=Tortoise microvirus 34 OX=2583137 PE=4 SV=1
MM1 pKa = 7.36EE2 pKa = 4.37VLMLVMEE9 pKa = 4.81VKK11 pKa = 10.88DD12 pKa = 3.81EE13 pKa = 4.06VRR15 pKa = 11.84PEE17 pKa = 3.89VPFRR21 pKa = 11.84SITDD25 pKa = 3.5LKK27 pKa = 11.17GYY29 pKa = 10.47LDD31 pKa = 3.68YY32 pKa = 11.44EE33 pKa = 4.51RR34 pKa = 11.84IEE36 pKa = 4.78DD37 pKa = 4.08EE38 pKa = 4.41GLVDD42 pKa = 4.65QDD44 pKa = 4.27YY45 pKa = 10.82EE46 pKa = 4.51SLSSIIARR54 pKa = 11.84MQRR57 pKa = 11.84GEE59 pKa = 4.31SVASRR64 pKa = 11.84NDD66 pKa = 3.2YY67 pKa = 10.87YY68 pKa = 11.46YY69 pKa = 10.78EE70 pKa = 4.61SDD72 pKa = 3.96MDD74 pKa = 5.46DD75 pKa = 3.62GDD77 pKa = 4.72LFEE80 pKa = 5.74SMNPLEE86 pKa = 5.78SPDD89 pKa = 4.53ADD91 pKa = 4.05LADD94 pKa = 4.44ASALMDD100 pKa = 5.05SIQGNTNVGGQGEE113 pKa = 4.87DD114 pKa = 3.46VPPATSPDD122 pKa = 3.54KK123 pKa = 11.01AIEE126 pKa = 4.17PSSDD130 pKa = 2.99GGALEE135 pKa = 4.04

Molecular weight: 14.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P8W9L8|A0A4P8W9L8_9VIRU DNA pilot protein OS=Tortoise microvirus 34 OX=2583137 PE=4 SV=1
MM1 pKa = 7.73RR2 pKa = 11.84SDD4 pKa = 3.26TAGVTEE10 pKa = 4.34RR11 pKa = 11.84RR12 pKa = 11.84VMADD16 pKa = 2.55IGLGGMLLGGGLLKK30 pKa = 11.02GLDD33 pKa = 3.9FGLSSWANSLNMSRR47 pKa = 11.84QHH49 pKa = 6.55GYY51 pKa = 11.37DD52 pKa = 3.37MNVAAANYY60 pKa = 8.0KK61 pKa = 8.46ATKK64 pKa = 10.76ALMAQQNAWSSPSNQMRR81 pKa = 11.84LFEE84 pKa = 4.54KK85 pKa = 10.78AGLNPNLVYY94 pKa = 10.45GHH96 pKa = 6.59QIPSASPSGNIVQNNARR113 pKa = 11.84SNINPGSASDD123 pKa = 4.91GLMQYY128 pKa = 11.61LSMKK132 pKa = 10.3NVAEE136 pKa = 4.32TNKK139 pKa = 10.06NLQSLTGLHH148 pKa = 7.01DD149 pKa = 4.05AQILKK154 pKa = 9.55TMAEE158 pKa = 3.92IEE160 pKa = 4.44EE161 pKa = 4.11IDD163 pKa = 3.91ARR165 pKa = 11.84TTGMDD170 pKa = 3.22INNLTDD176 pKa = 3.35YY177 pKa = 11.05LRR179 pKa = 11.84GHH181 pKa = 6.91MYY183 pKa = 10.85RR184 pKa = 11.84LTGSVDD190 pKa = 3.24GSSLQSLLAGPIRR203 pKa = 11.84MAMASAVGSFEE214 pKa = 5.57DD215 pKa = 3.33RR216 pKa = 11.84GKK218 pKa = 10.7DD219 pKa = 2.95RR220 pKa = 11.84SHH222 pKa = 7.29EE223 pKa = 3.89IRR225 pKa = 11.84RR226 pKa = 11.84LRR228 pKa = 11.84DD229 pKa = 3.16SFSRR233 pKa = 11.84RR234 pKa = 11.84SKK236 pKa = 10.7SEE238 pKa = 3.27PKK240 pKa = 10.58GRR242 pKa = 11.84VIQVDD247 pKa = 3.86GKK249 pKa = 10.57RR250 pKa = 11.84PNWLSRR256 pKa = 11.84RR257 pKa = 11.84FYY259 pKa = 11.05DD260 pKa = 4.05LSEE263 pKa = 4.25FFDD266 pKa = 4.0RR267 pKa = 11.84FF268 pKa = 3.37

Molecular weight: 29.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1215

135

513

303.8

34.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.749 ± 0.633

1.481 ± 1.049

6.42 ± 1.407

5.844 ± 1.75

3.868 ± 0.591

8.066 ± 0.401

1.893 ± 0.378

3.457 ± 0.303

5.103 ± 1.003

8.889 ± 0.416

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.128 ± 0.821

4.115 ± 0.998

4.856 ± 0.876

3.457 ± 0.31

7.572 ± 0.937

7.984 ± 1.065

4.198 ± 1.075

7.078 ± 1.029

1.317 ± 0.294

4.527 ± 0.721
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski