Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) (Cereibacter sphaeroides)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Cereibacter; Cereibacter sphaeroides

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4285 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3IZQ1|Q3IZQ1_RHOS4 ABC dipeptide transporter ATPase subunit DppD OS=Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) OX=272943 GN=DppD PE=4 SV=1
MM1 pKa = 7.15KK2 pKa = 10.32QILLATTALVMTAGVAAAEE21 pKa = 4.4ISFSGYY27 pKa = 11.08AEE29 pKa = 4.0MGVVGGGDD37 pKa = 3.67DD38 pKa = 3.46APARR42 pKa = 11.84AGHH45 pKa = 6.33AAGGQTEE52 pKa = 3.98FHH54 pKa = 6.87NDD56 pKa = 2.64FNLIVTMTSEE66 pKa = 4.4TDD68 pKa = 2.71TGLAFGASVEE78 pKa = 4.05ISKK81 pKa = 11.22DD82 pKa = 3.39EE83 pKa = 4.29GDD85 pKa = 3.91SNGNFAADD93 pKa = 3.42NEE95 pKa = 4.37AAFISGAFGTLTMGEE110 pKa = 4.1IDD112 pKa = 5.24GAMDD116 pKa = 3.25WAMTEE121 pKa = 4.11NVGNPGTIGDD131 pKa = 5.01DD132 pKa = 3.64EE133 pKa = 4.64TTHH136 pKa = 6.55AGYY139 pKa = 10.39FGAYY143 pKa = 10.13GDD145 pKa = 4.24GKK147 pKa = 10.92YY148 pKa = 10.71DD149 pKa = 3.59NQIARR154 pKa = 11.84YY155 pKa = 8.43EE156 pKa = 4.03YY157 pKa = 10.22SFGDD161 pKa = 3.37FGVAISAEE169 pKa = 4.13LDD171 pKa = 3.56DD172 pKa = 4.69TDD174 pKa = 4.43TNGDD178 pKa = 3.59GYY180 pKa = 11.44AIGAKK185 pKa = 10.2YY186 pKa = 10.37KK187 pKa = 10.92GDD189 pKa = 3.45FGGFGMGFGLAYY201 pKa = 8.95QTFEE205 pKa = 3.93TDD207 pKa = 3.41VLVGLGSQEE216 pKa = 3.74AALVGGDD223 pKa = 3.83VEE225 pKa = 5.16LLGASVNADD234 pKa = 3.4FNNGFVAGLAYY245 pKa = 9.79TDD247 pKa = 3.96ISSDD251 pKa = 3.54AQDD254 pKa = 3.91GNHH257 pKa = 6.81LGLSAGFTTGAFQIGANYY275 pKa = 10.32GVFDD279 pKa = 4.63MDD281 pKa = 3.58VQDD284 pKa = 4.89KK285 pKa = 10.34VKK287 pKa = 10.3GWGVAAAYY295 pKa = 10.2DD296 pKa = 3.72LGGGLKK302 pKa = 10.43LHH304 pKa = 6.84GGYY307 pKa = 10.43GDD309 pKa = 3.97SDD311 pKa = 3.55IDD313 pKa = 4.0GVDD316 pKa = 3.65GDD318 pKa = 4.41FEE320 pKa = 4.82TYY322 pKa = 10.47SVGLSMSFF330 pKa = 3.27

Molecular weight: 33.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q3IYZ4|Q3IYZ4_RHOS4 Histidine kinase OS=Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) OX=272943 GN=RSP_1056 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.39GGRR28 pKa = 11.84LVLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.06GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4285

0

4285

1331697

20

3634

310.8

33.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.681 ± 0.068

0.87 ± 0.012

5.357 ± 0.038

6.265 ± 0.033

3.483 ± 0.024

8.97 ± 0.036

1.956 ± 0.017

4.527 ± 0.026

2.43 ± 0.031

10.871 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.554 ± 0.018

2.037 ± 0.021

5.595 ± 0.037

2.874 ± 0.022

7.935 ± 0.041

4.886 ± 0.029

5.151 ± 0.034

7.225 ± 0.029

1.395 ± 0.017

1.936 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski