Amino acid dipepetide frequency for Streptococcus phage SPQS1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.147AlaAla: 6.147 ± 1.233
0.632AlaCys: 0.632 ± 0.248
4.309AlaAsp: 4.309 ± 0.637
5.86AlaGlu: 5.86 ± 0.579
3.102AlaPhe: 3.102 ± 0.405
4.136AlaGly: 4.136 ± 0.685
0.747AlaHis: 0.747 ± 0.241
5.285AlaIle: 5.285 ± 0.462
4.711AlaLys: 4.711 ± 0.677
6.204AlaLeu: 6.204 ± 0.649
2.183AlaMet: 2.183 ± 0.417
3.677AlaAsn: 3.677 ± 0.5
1.838AlaPro: 1.838 ± 0.297
2.24AlaGln: 2.24 ± 0.526
3.619AlaArg: 3.619 ± 0.51
4.251AlaSer: 4.251 ± 0.422
4.194AlaThr: 4.194 ± 0.61
4.883AlaVal: 4.883 ± 0.679
0.517AlaTrp: 0.517 ± 0.168
3.16AlaTyr: 3.16 ± 0.498
0.0AlaXaa: 0.0 ± 0.0
Cys
0.46CysAla: 0.46 ± 0.162
0.23CysCys: 0.23 ± 0.144
0.402CysAsp: 0.402 ± 0.15
0.804CysGlu: 0.804 ± 0.235
0.345CysPhe: 0.345 ± 0.135
0.862CysGly: 0.862 ± 0.269
0.115CysHis: 0.115 ± 0.083
0.517CysIle: 0.517 ± 0.19
0.46CysLys: 0.46 ± 0.23
0.747CysLeu: 0.747 ± 0.236
0.287CysMet: 0.287 ± 0.139
0.115CysAsn: 0.115 ± 0.086
0.057CysPro: 0.057 ± 0.056
0.345CysGln: 0.345 ± 0.153
0.23CysArg: 0.23 ± 0.096
0.46CysSer: 0.46 ± 0.14
0.23CysThr: 0.23 ± 0.129
0.517CysVal: 0.517 ± 0.188
0.057CysTrp: 0.057 ± 0.05
0.402CysTyr: 0.402 ± 0.139
0.0CysXaa: 0.0 ± 0.0
Asp
4.079AspAla: 4.079 ± 0.574
0.632AspCys: 0.632 ± 0.188
3.16AspAsp: 3.16 ± 0.754
4.194AspGlu: 4.194 ± 0.452
3.792AspPhe: 3.792 ± 0.36
3.792AspGly: 3.792 ± 0.485
0.517AspHis: 0.517 ± 0.186
4.136AspIle: 4.136 ± 0.54
4.309AspLys: 4.309 ± 0.474
4.998AspLeu: 4.998 ± 0.436
1.723AspMet: 1.723 ± 0.415
3.16AspAsn: 3.16 ± 0.417
1.264AspPro: 1.264 ± 0.259
1.206AspGln: 1.206 ± 0.279
2.068AspArg: 2.068 ± 0.381
3.677AspSer: 3.677 ± 0.408
4.653AspThr: 4.653 ± 0.755
4.366AspVal: 4.366 ± 0.473
1.092AspTrp: 1.092 ± 0.273
3.677AspTyr: 3.677 ± 0.458
0.0AspXaa: 0.0 ± 0.0
Glu
8.502GluAla: 8.502 ± 0.757
0.517GluCys: 0.517 ± 0.166
5.515GluAsp: 5.515 ± 0.527
10.685GluGlu: 10.685 ± 0.996
3.849GluPhe: 3.849 ± 0.666
5.745GluGly: 5.745 ± 0.622
0.919GluHis: 0.919 ± 0.234
4.309GluIle: 4.309 ± 0.458
5.17GluLys: 5.17 ± 0.649
7.641GluLeu: 7.641 ± 0.682
2.413GluMet: 2.413 ± 0.354
3.792GluAsn: 3.792 ± 0.492
2.068GluPro: 2.068 ± 0.331
3.389GluGln: 3.389 ± 0.553
4.481GluArg: 4.481 ± 0.452
5.285GluSer: 5.285 ± 0.606
3.849GluThr: 3.849 ± 0.586
6.09GluVal: 6.09 ± 0.573
1.321GluTrp: 1.321 ± 0.273
3.389GluTyr: 3.389 ± 0.39
0.0GluXaa: 0.0 ± 0.0
Phe
2.47PheAla: 2.47 ± 0.341
0.345PheCys: 0.345 ± 0.136
3.045PheAsp: 3.045 ± 0.39
3.447PheGlu: 3.447 ± 0.423
1.666PhePhe: 1.666 ± 0.283
3.045PheGly: 3.045 ± 0.472
0.689PheHis: 0.689 ± 0.185
3.102PheIle: 3.102 ± 0.442
3.677PheLys: 3.677 ± 0.411
3.275PheLeu: 3.275 ± 0.46
1.034PheMet: 1.034 ± 0.275
2.24PheAsn: 2.24 ± 0.306
1.436PhePro: 1.436 ± 0.348
1.149PheGln: 1.149 ± 0.244
1.494PheArg: 1.494 ± 0.269
2.872PheSer: 2.872 ± 0.526
2.987PheThr: 2.987 ± 0.387
2.413PheVal: 2.413 ± 0.376
0.574PheTrp: 0.574 ± 0.21
1.896PheTyr: 1.896 ± 0.407
0.0PheXaa: 0.0 ± 0.0
Gly
3.504GlyAla: 3.504 ± 0.463
0.46GlyCys: 0.46 ± 0.21
3.389GlyAsp: 3.389 ± 0.5
4.079GlyGlu: 4.079 ± 0.465
3.562GlyPhe: 3.562 ± 0.468
3.677GlyGly: 3.677 ± 0.793
1.092GlyHis: 1.092 ± 0.202
4.826GlyIle: 4.826 ± 0.63
5.458GlyLys: 5.458 ± 0.756
4.309GlyLeu: 4.309 ± 0.511
1.953GlyMet: 1.953 ± 0.333
3.102GlyAsn: 3.102 ± 0.421
0.115GlyPro: 0.115 ± 0.084
1.781GlyGln: 1.781 ± 0.383
2.355GlyArg: 2.355 ± 0.341
3.275GlySer: 3.275 ± 0.342
4.366GlyThr: 4.366 ± 0.706
4.021GlyVal: 4.021 ± 0.545
1.092GlyTrp: 1.092 ± 0.311
3.045GlyTyr: 3.045 ± 0.363
0.0GlyXaa: 0.0 ± 0.0
His
1.264HisAla: 1.264 ± 0.305
0.23HisCys: 0.23 ± 0.112
0.574HisAsp: 0.574 ± 0.195
1.149HisGlu: 1.149 ± 0.243
0.862HisPhe: 0.862 ± 0.182
0.977HisGly: 0.977 ± 0.224
0.287HisHis: 0.287 ± 0.142
1.206HisIle: 1.206 ± 0.251
1.149HisLys: 1.149 ± 0.256
1.494HisLeu: 1.494 ± 0.308
0.23HisMet: 0.23 ± 0.108
1.149HisAsn: 1.149 ± 0.242
0.632HisPro: 0.632 ± 0.186
0.574HisGln: 0.574 ± 0.205
0.747HisArg: 0.747 ± 0.207
0.804HisSer: 0.804 ± 0.241
0.46HisThr: 0.46 ± 0.13
0.919HisVal: 0.919 ± 0.206
0.057HisTrp: 0.057 ± 0.051
0.804HisTyr: 0.804 ± 0.233
0.0HisXaa: 0.0 ± 0.0
Ile
4.653IleAla: 4.653 ± 0.512
0.632IleCys: 0.632 ± 0.2
4.481IleAsp: 4.481 ± 0.429
5.228IleGlu: 5.228 ± 0.534
2.011IlePhe: 2.011 ± 0.344
3.447IleGly: 3.447 ± 0.386
1.609IleHis: 1.609 ± 0.29
3.102IleIle: 3.102 ± 0.477
5.17IleLys: 5.17 ± 0.599
4.079IleLeu: 4.079 ± 0.641
1.723IleMet: 1.723 ± 0.295
4.711IleAsn: 4.711 ± 0.465
2.011IlePro: 2.011 ± 0.326
2.47IleGln: 2.47 ± 0.498
2.872IleArg: 2.872 ± 0.469
3.504IleSer: 3.504 ± 0.459
4.079IleThr: 4.079 ± 0.736
3.792IleVal: 3.792 ± 0.555
0.632IleTrp: 0.632 ± 0.218
2.872IleTyr: 2.872 ± 0.391
0.0IleXaa: 0.0 ± 0.0
Lys
7.181LysAla: 7.181 ± 0.669
0.287LysCys: 0.287 ± 0.12
5.343LysAsp: 5.343 ± 0.48
7.583LysGlu: 7.583 ± 0.855
2.93LysPhe: 2.93 ± 0.351
4.596LysGly: 4.596 ± 0.524
1.551LysHis: 1.551 ± 0.351
4.194LysIle: 4.194 ± 0.441
6.262LysLys: 6.262 ± 0.828
6.779LysLeu: 6.779 ± 0.662
2.643LysMet: 2.643 ± 0.511
2.987LysAsn: 2.987 ± 0.509
2.815LysPro: 2.815 ± 0.421
2.987LysGln: 2.987 ± 0.455
3.389LysArg: 3.389 ± 0.508
4.079LysSer: 4.079 ± 0.546
4.136LysThr: 4.136 ± 0.473
5.343LysVal: 5.343 ± 0.512
1.034LysTrp: 1.034 ± 0.189
2.355LysTyr: 2.355 ± 0.504
0.0LysXaa: 0.0 ± 0.0
Leu
5.285LeuAla: 5.285 ± 0.536
0.517LeuCys: 0.517 ± 0.18
4.998LeuAsp: 4.998 ± 0.484
9.249LeuGlu: 9.249 ± 0.925
2.758LeuPhe: 2.758 ± 0.397
4.768LeuGly: 4.768 ± 0.611
1.436LeuHis: 1.436 ± 0.274
5.63LeuIle: 5.63 ± 0.643
6.779LeuLys: 6.779 ± 0.74
7.009LeuLeu: 7.009 ± 0.955
2.298LeuMet: 2.298 ± 0.315
4.021LeuAsn: 4.021 ± 0.475
2.815LeuPro: 2.815 ± 0.4
3.447LeuGln: 3.447 ± 0.506
3.102LeuArg: 3.102 ± 0.379
5.687LeuSer: 5.687 ± 0.512
6.319LeuThr: 6.319 ± 0.647
5.285LeuVal: 5.285 ± 0.618
0.689LeuTrp: 0.689 ± 0.173
2.7LeuTyr: 2.7 ± 0.464
0.0LeuXaa: 0.0 ± 0.0
Met
2.413MetAla: 2.413 ± 0.435
0.23MetCys: 0.23 ± 0.123
2.011MetAsp: 2.011 ± 0.33
2.987MetGlu: 2.987 ± 0.383
1.034MetPhe: 1.034 ± 0.28
1.034MetGly: 1.034 ± 0.327
0.345MetHis: 0.345 ± 0.139
1.551MetIle: 1.551 ± 0.328
2.183MetLys: 2.183 ± 0.35
2.643MetLeu: 2.643 ± 0.417
0.287MetMet: 0.287 ± 0.14
1.666MetAsn: 1.666 ± 0.304
1.034MetPro: 1.034 ± 0.218
0.689MetGln: 0.689 ± 0.199
1.264MetArg: 1.264 ± 0.277
1.609MetSer: 1.609 ± 0.273
1.609MetThr: 1.609 ± 0.334
1.206MetVal: 1.206 ± 0.231
0.23MetTrp: 0.23 ± 0.114
0.804MetTyr: 0.804 ± 0.207
0.0MetXaa: 0.0 ± 0.0
Asn
3.16AsnAla: 3.16 ± 0.471
0.402AsnCys: 0.402 ± 0.145
2.298AsnAsp: 2.298 ± 0.363
4.538AsnGlu: 4.538 ± 0.474
2.011AsnPhe: 2.011 ± 0.282
3.275AsnGly: 3.275 ± 0.422
0.977AsnHis: 0.977 ± 0.246
3.562AsnIle: 3.562 ± 0.394
4.309AsnLys: 4.309 ± 0.514
3.677AsnLeu: 3.677 ± 0.443
1.206AsnMet: 1.206 ± 0.251
1.896AsnAsn: 1.896 ± 0.388
2.872AsnPro: 2.872 ± 0.383
2.068AsnGln: 2.068 ± 0.398
2.183AsnArg: 2.183 ± 0.294
3.504AsnSer: 3.504 ± 0.382
2.987AsnThr: 2.987 ± 0.431
2.93AsnVal: 2.93 ± 0.413
0.574AsnTrp: 0.574 ± 0.179
1.781AsnTyr: 1.781 ± 0.379
0.0AsnXaa: 0.0 ± 0.0
Pro
1.781ProAla: 1.781 ± 0.415
0.287ProCys: 0.287 ± 0.158
1.551ProAsp: 1.551 ± 0.259
3.389ProGlu: 3.389 ± 0.521
1.494ProPhe: 1.494 ± 0.327
0.172ProGly: 0.172 ± 0.09
0.574ProHis: 0.574 ± 0.181
2.068ProIle: 2.068 ± 0.302
3.045ProLys: 3.045 ± 0.398
2.7ProLeu: 2.7 ± 0.445
0.804ProMet: 0.804 ± 0.227
1.666ProAsn: 1.666 ± 0.391
0.977ProPro: 0.977 ± 0.262
0.747ProGln: 0.747 ± 0.243
1.149ProArg: 1.149 ± 0.333
2.872ProSer: 2.872 ± 0.381
2.011ProThr: 2.011 ± 0.302
1.551ProVal: 1.551 ± 0.29
0.402ProTrp: 0.402 ± 0.164
1.551ProTyr: 1.551 ± 0.298
0.0ProXaa: 0.0 ± 0.0
Gln
2.758GlnAla: 2.758 ± 0.478
0.172GlnCys: 0.172 ± 0.111
1.896GlnAsp: 1.896 ± 0.372
3.102GlnGlu: 3.102 ± 0.448
1.321GlnPhe: 1.321 ± 0.268
2.47GlnGly: 2.47 ± 0.351
0.632GlnHis: 0.632 ± 0.199
1.551GlnIle: 1.551 ± 0.422
2.183GlnLys: 2.183 ± 0.353
3.562GlnLeu: 3.562 ± 0.49
0.689GlnMet: 0.689 ± 0.259
1.321GlnAsn: 1.321 ± 0.257
1.092GlnPro: 1.092 ± 0.226
1.896GlnGln: 1.896 ± 0.448
1.609GlnArg: 1.609 ± 0.282
2.126GlnSer: 2.126 ± 0.386
2.528GlnThr: 2.528 ± 0.438
2.47GlnVal: 2.47 ± 0.372
0.517GlnTrp: 0.517 ± 0.143
1.494GlnTyr: 1.494 ± 0.346
0.0GlnXaa: 0.0 ± 0.0
Arg
2.815ArgAla: 2.815 ± 0.489
0.402ArgCys: 0.402 ± 0.15
2.528ArgAsp: 2.528 ± 0.368
2.815ArgGlu: 2.815 ± 0.318
2.068ArgPhe: 2.068 ± 0.347
2.643ArgGly: 2.643 ± 0.365
0.919ArgHis: 0.919 ± 0.224
3.16ArgIle: 3.16 ± 0.461
3.332ArgLys: 3.332 ± 0.426
4.136ArgLeu: 4.136 ± 0.494
0.747ArgMet: 0.747 ± 0.177
1.953ArgAsn: 1.953 ± 0.351
1.723ArgPro: 1.723 ± 0.366
1.494ArgGln: 1.494 ± 0.305
2.011ArgArg: 2.011 ± 0.395
1.953ArgSer: 1.953 ± 0.361
1.896ArgThr: 1.896 ± 0.24
2.7ArgVal: 2.7 ± 0.36
0.345ArgTrp: 0.345 ± 0.148
1.838ArgTyr: 1.838 ± 0.371
0.0ArgXaa: 0.0 ± 0.0
Ser
2.872SerAla: 2.872 ± 0.45
0.402SerCys: 0.402 ± 0.146
3.504SerAsp: 3.504 ± 0.42
4.768SerGlu: 4.768 ± 0.53
2.758SerPhe: 2.758 ± 0.355
3.734SerGly: 3.734 ± 0.434
0.919SerHis: 0.919 ± 0.214
4.021SerIle: 4.021 ± 0.503
6.147SerLys: 6.147 ± 0.558
5.745SerLeu: 5.745 ± 0.629
1.723SerMet: 1.723 ± 0.31
3.045SerAsn: 3.045 ± 0.365
1.723SerPro: 1.723 ± 0.231
2.011SerGln: 2.011 ± 0.418
2.47SerArg: 2.47 ± 0.386
3.389SerSer: 3.389 ± 0.437
3.504SerThr: 3.504 ± 0.401
4.079SerVal: 4.079 ± 0.433
0.804SerTrp: 0.804 ± 0.222
3.275SerTyr: 3.275 ± 0.428
0.0SerXaa: 0.0 ± 0.0
Thr
4.826ThrAla: 4.826 ± 0.756
0.46ThrCys: 0.46 ± 0.168
3.849ThrAsp: 3.849 ± 0.413
3.906ThrGlu: 3.906 ± 0.382
2.7ThrPhe: 2.7 ± 0.343
3.792ThrGly: 3.792 ± 0.613
0.862ThrHis: 0.862 ± 0.23
4.021ThrIle: 4.021 ± 0.548
3.619ThrLys: 3.619 ± 0.38
6.032ThrLeu: 6.032 ± 0.595
1.494ThrMet: 1.494 ± 0.284
2.872ThrAsn: 2.872 ± 0.527
2.815ThrPro: 2.815 ± 0.472
1.723ThrGln: 1.723 ± 0.32
1.838ThrArg: 1.838 ± 0.366
3.619ThrSer: 3.619 ± 0.61
3.16ThrThr: 3.16 ± 0.465
5.17ThrVal: 5.17 ± 0.637
0.977ThrTrp: 0.977 ± 0.265
2.585ThrTyr: 2.585 ± 0.381
0.0ThrXaa: 0.0 ± 0.0
Val
4.481ValAla: 4.481 ± 0.563
0.632ValCys: 0.632 ± 0.205
4.251ValAsp: 4.251 ± 0.417
5.285ValGlu: 5.285 ± 0.526
2.7ValPhe: 2.7 ± 0.383
4.021ValGly: 4.021 ± 0.527
0.689ValHis: 0.689 ± 0.168
4.194ValIle: 4.194 ± 0.455
6.147ValLys: 6.147 ± 0.609
5.113ValLeu: 5.113 ± 0.501
1.551ValMet: 1.551 ± 0.292
3.332ValAsn: 3.332 ± 0.407
2.068ValPro: 2.068 ± 0.376
2.7ValGln: 2.7 ± 0.353
2.585ValArg: 2.585 ± 0.415
4.021ValSer: 4.021 ± 0.492
3.849ValThr: 3.849 ± 0.494
4.079ValVal: 4.079 ± 0.569
1.264ValTrp: 1.264 ± 0.269
2.413ValTyr: 2.413 ± 0.403
0.0ValXaa: 0.0 ± 0.0
Trp
0.747TrpAla: 0.747 ± 0.187
0.172TrpCys: 0.172 ± 0.098
0.862TrpAsp: 0.862 ± 0.227
1.494TrpGlu: 1.494 ± 0.295
0.574TrpPhe: 0.574 ± 0.218
1.034TrpGly: 1.034 ± 0.294
0.172TrpHis: 0.172 ± 0.108
0.862TrpIle: 0.862 ± 0.24
0.747TrpLys: 0.747 ± 0.167
0.574TrpLeu: 0.574 ± 0.17
0.115TrpMet: 0.115 ± 0.069
1.092TrpAsn: 1.092 ± 0.236
0.057TrpPro: 0.057 ± 0.062
0.689TrpGln: 0.689 ± 0.19
0.23TrpArg: 0.23 ± 0.107
1.149TrpSer: 1.149 ± 0.232
0.804TrpThr: 0.804 ± 0.243
0.632TrpVal: 0.632 ± 0.15
0.057TrpTrp: 0.057 ± 0.059
0.747TrpTyr: 0.747 ± 0.196
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.298TyrAla: 2.298 ± 0.406
0.0TyrCys: 0.0 ± 0.0
2.528TyrAsp: 2.528 ± 0.415
4.366TyrGlu: 4.366 ± 0.667
1.321TyrPhe: 1.321 ± 0.285
2.24TyrGly: 2.24 ± 0.435
0.517TyrHis: 0.517 ± 0.192
1.781TyrIle: 1.781 ± 0.303
3.619TyrLys: 3.619 ± 0.407
4.079TyrLeu: 4.079 ± 0.472
1.723TyrMet: 1.723 ± 0.332
2.413TyrAsn: 2.413 ± 0.458
1.379TyrPro: 1.379 ± 0.295
1.781TyrGln: 1.781 ± 0.324
1.781TyrArg: 1.781 ± 0.367
2.758TyrSer: 2.758 ± 0.389
2.7TyrThr: 2.7 ± 0.443
2.93TyrVal: 2.93 ± 0.373
0.574TyrTrp: 0.574 ± 0.185
2.126TyrTyr: 2.126 ± 0.387
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 104 proteins (17408 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski