Nocardiopsis sp. CNR-923

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Nocardiopsaceae; Nocardiopsis; unclassified Nocardiopsis

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3756 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q9TMI5|A0A1Q9TMI5_9ACTN Uncharacterized protein OS=Nocardiopsis sp. CNR-923 OX=1904965 GN=BJF83_20670 PE=4 SV=1
MM1 pKa = 7.07TRR3 pKa = 11.84HH4 pKa = 5.43PVARR8 pKa = 11.84SLGTTALSLTLVLAGAGLVSADD30 pKa = 3.67TTTGDD35 pKa = 3.31ISPEE39 pKa = 3.37QMAAMRR45 pKa = 11.84AAFGLSEE52 pKa = 4.61SGVDD56 pKa = 4.94DD57 pKa = 4.37LFEE60 pKa = 4.42AQEE63 pKa = 4.07EE64 pKa = 4.4ATEE67 pKa = 4.3TEE69 pKa = 3.96AEE71 pKa = 4.04LRR73 pKa = 11.84EE74 pKa = 4.16DD75 pKa = 3.71LGADD79 pKa = 3.62FGGAFFDD86 pKa = 5.14VDD88 pKa = 3.97TLDD91 pKa = 3.68LTVQVTDD98 pKa = 4.84PSASDD103 pKa = 3.39AVRR106 pKa = 11.84DD107 pKa = 3.89AGAVPEE113 pKa = 4.25VVDD116 pKa = 3.67NGEE119 pKa = 4.18AEE121 pKa = 4.05LAAGVEE127 pKa = 4.22ALDD130 pKa = 4.95AVDD133 pKa = 4.53EE134 pKa = 4.73VPATVHH140 pKa = 5.23GWYY143 pKa = 10.38ADD145 pKa = 3.71TVRR148 pKa = 11.84DD149 pKa = 3.63AVVIEE154 pKa = 4.14AAPGHH159 pKa = 5.52EE160 pKa = 4.08AEE162 pKa = 4.61ARR164 pKa = 11.84ALAEE168 pKa = 4.07RR169 pKa = 11.84AGVEE173 pKa = 3.51ADD175 pKa = 3.67TVVEE179 pKa = 4.25EE180 pKa = 4.68SVGTPTTYY188 pKa = 11.51ADD190 pKa = 3.09IVGGNPYY197 pKa = 10.33YY198 pKa = 10.38FQQDD202 pKa = 3.61GGWYY206 pKa = 7.85VCSVGFGVVGGYY218 pKa = 7.49VTAGHH223 pKa = 6.65CGDD226 pKa = 4.61EE227 pKa = 5.87GSDD230 pKa = 3.1TWLDD234 pKa = 3.57VPGTQQIGTVAGSSFPTSDD253 pKa = 3.66SAWVEE258 pKa = 4.11VTGSSTVPTAEE269 pKa = 3.92VNDD272 pKa = 4.11YY273 pKa = 11.25EE274 pKa = 5.19GGTVTVTGSTEE285 pKa = 4.02APVGASVCRR294 pKa = 11.84SGQTTGWHH302 pKa = 6.12CGVIEE307 pKa = 5.26AKK309 pKa = 10.48DD310 pKa = 3.32QTVRR314 pKa = 11.84YY315 pKa = 9.23AGNLVVYY322 pKa = 10.27GLTRR326 pKa = 11.84TSACAEE332 pKa = 4.31GGDD335 pKa = 5.2SGGSWLAGTEE345 pKa = 4.19AQGVTSGGSGNCTFGGTTFFQPLNPILDD373 pKa = 3.82EE374 pKa = 4.24WDD376 pKa = 3.38LTLLTGG382 pKa = 4.58

Molecular weight: 39.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q9TFL8|A0A1Q9TFL8_9ACTN 50S ribosomal protein L30 OS=Nocardiopsis sp. CNR-923 OX=1904965 GN=rpmD PE=3 SV=1
MM1 pKa = 7.74SKK3 pKa = 8.93RR4 pKa = 11.84TYY6 pKa = 10.0QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIASRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.54GRR40 pKa = 11.84SALTVSHH47 pKa = 7.04

Molecular weight: 5.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3756

0

3756

1021308

37

2136

271.9

29.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.789 ± 0.047

0.82 ± 0.013

6.388 ± 0.037

6.337 ± 0.037

2.7 ± 0.023

9.196 ± 0.041

2.434 ± 0.022

3.089 ± 0.024

1.549 ± 0.026

10.172 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.885 ± 0.017

1.697 ± 0.02

5.971 ± 0.039

2.532 ± 0.019

8.858 ± 0.048

5.211 ± 0.031

5.872 ± 0.031

8.975 ± 0.043

1.588 ± 0.021

1.935 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski