Sinapis alba cryptic virus 1
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0U3AHS1|A0A0U3AHS1_9VIRU Capsid protein OS=Sinapis alba cryptic virus 1 OX=1768874 PE=4 SV=1
MM1 pKa = 6.52 TTGDD5 pKa = 3.87 RR6 pKa = 11.84 VTPTDD11 pKa = 4.7 GITTVDD17 pKa = 3.63 APTATLEE24 pKa = 4.21 AKK26 pKa = 10.33 LDD28 pKa = 3.96 SSSTAYY34 pKa = 10.46 LKK36 pKa = 10.85 SATDD40 pKa = 4.05 FLTQSSRR47 pKa = 11.84 IGTLRR52 pKa = 11.84 TSYY55 pKa = 10.77 KK56 pKa = 9.84 DD57 pKa = 3.04 RR58 pKa = 11.84 RR59 pKa = 11.84 RR60 pKa = 11.84 YY61 pKa = 10.25 VYY63 pKa = 10.56 LKK65 pKa = 9.52 SQAMFDD71 pKa = 4.05 SLVEE75 pKa = 4.22 LYY77 pKa = 10.89 SLLFTTQWQVFRR89 pKa = 11.84 RR90 pKa = 11.84 FTDD93 pKa = 3.12 IFVPPANVTATRR105 pKa = 11.84 YY106 pKa = 9.92 LAMNYY111 pKa = 9.6 ISMWIQDD118 pKa = 3.9 TYY120 pKa = 11.75 AMNRR124 pKa = 11.84 AALMKK129 pKa = 10.52 LSVVALNDD137 pKa = 3.63 RR138 pKa = 11.84 FPVEE142 pKa = 4.08 IPIVGYY148 pKa = 10.64 QYY150 pKa = 11.25 DD151 pKa = 3.96 AYY153 pKa = 10.65 LAHH156 pKa = 7.22 LSAALRR162 pKa = 11.84 PTLTKK167 pKa = 10.98 HH168 pKa = 5.5 MMEE171 pKa = 4.18 DD172 pKa = 3.99 TLWIPCFTRR181 pKa = 11.84 SPNFQVEE188 pKa = 4.74 SPFGITGYY196 pKa = 10.38 VINDD200 pKa = 3.63 VIFTGAMNIMKK211 pKa = 9.62 EE212 pKa = 3.89 RR213 pKa = 11.84 KK214 pKa = 7.94 LWRR217 pKa = 11.84 IIDD220 pKa = 3.92 LPTNVLGRR228 pKa = 11.84 PSWLFDD234 pKa = 2.91 WHH236 pKa = 7.56 DD237 pKa = 4.23 DD238 pKa = 4.37 DD239 pKa = 6.33 RR240 pKa = 11.84 VCAWFPMEE248 pKa = 5.61 GNFTMDD254 pKa = 4.56 DD255 pKa = 3.06 VTMAYY260 pKa = 9.43 ILGVACTPKK269 pKa = 10.49 LAPCDD274 pKa = 3.72 VDD276 pKa = 3.14 DD277 pKa = 4.05 WQYY280 pKa = 11.13 FPNDD284 pKa = 3.63 VVPANLDD291 pKa = 3.17 PYY293 pKa = 11.64 AFLRR297 pKa = 11.84 LTNRR301 pKa = 11.84 RR302 pKa = 11.84 FHH304 pKa = 6.56 GGYY307 pKa = 10.32 EE308 pKa = 3.86 IRR310 pKa = 11.84 TLDD313 pKa = 3.74 AANMPGQTGTTTDD326 pKa = 2.66 SRR328 pKa = 11.84 KK329 pKa = 9.66 RR330 pKa = 11.84 PRR332 pKa = 11.84 PGEE335 pKa = 4.22 STSAPALPQTSEE347 pKa = 4.43 PQTQMQVDD355 pKa = 4.76 TPPAPKK361 pKa = 9.79 PRR363 pKa = 11.84 FRR365 pKa = 11.84 LVDD368 pKa = 3.15 WTYY371 pKa = 11.16 HH372 pKa = 5.06 YY373 pKa = 11.24 LVVLNKK379 pKa = 9.85 EE380 pKa = 3.78 DD381 pKa = 3.49 HH382 pKa = 6.28 TRR384 pKa = 11.84 FAALKK389 pKa = 9.08 SICWNN394 pKa = 3.46
Molecular weight: 45.06 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.167
IPC2_protein 6.173
IPC_protein 6.313
Toseland 6.504
ProMoST 6.678
Dawson 6.678
Bjellqvist 6.605
Wikipedia 6.678
Rodwell 6.664
Grimsley 6.605
Solomon 6.678
Lehninger 6.678
Nozaki 6.912
DTASelect 7.088
Thurlkill 7.102
EMBOSS 7.102
Sillero 7.059
Patrickios 4.075
IPC_peptide 6.693
IPC2_peptide 6.854
IPC2.peptide.svr19 6.802
Protein with the highest isoelectric point:
>tr|A0A0U3AHS1|A0A0U3AHS1_9VIRU Capsid protein OS=Sinapis alba cryptic virus 1 OX=1768874 PE=4 SV=1
MM1 pKa = 7.6 RR2 pKa = 11.84 PSITGYY8 pKa = 10.77 DD9 pKa = 3.66 YY10 pKa = 11.72 TNFTQDD16 pKa = 3.65 LLKK19 pKa = 10.39 SDD21 pKa = 4.06 RR22 pKa = 11.84 KK23 pKa = 9.53 HH24 pKa = 5.14 PHH26 pKa = 3.57 VVRR29 pKa = 11.84 RR30 pKa = 11.84 EE31 pKa = 3.7 TATTYY36 pKa = 11.01 RR37 pKa = 11.84 DD38 pKa = 3.3 DD39 pKa = 3.86 FAFKK43 pKa = 10.48 EE44 pKa = 4.54 VISLDD49 pKa = 3.07 RR50 pKa = 11.84 LAYY53 pKa = 8.99 IQRR56 pKa = 11.84 LEE58 pKa = 3.95 GWSRR62 pKa = 11.84 SYY64 pKa = 11.09 YY65 pKa = 10.56 LPEE68 pKa = 3.89 KK69 pKa = 10.34 HH70 pKa = 6.85 LEE72 pKa = 4.03 ALLQYY77 pKa = 7.38 ATPNVPCTALNLNVYY92 pKa = 9.02 RR93 pKa = 11.84 QAIQVVEE100 pKa = 4.0 NGLRR104 pKa = 11.84 SLQPVRR110 pKa = 11.84 AFDD113 pKa = 4.02 VLTEE117 pKa = 4.46 LNQISYY123 pKa = 9.09 KK124 pKa = 9.82 QSSAAGYY131 pKa = 10.32 DD132 pKa = 3.61 YY133 pKa = 10.87 IGAKK137 pKa = 10.36 GPIDD141 pKa = 3.9 GEE143 pKa = 4.05 NHH145 pKa = 5.27 KK146 pKa = 10.57 RR147 pKa = 11.84 AISRR151 pKa = 11.84 AKK153 pKa = 9.39 AVLWSVVKK161 pKa = 10.64 EE162 pKa = 4.03 DD163 pKa = 5.04 GEE165 pKa = 4.89 GIDD168 pKa = 3.87 HH169 pKa = 7.55 AIEE172 pKa = 4.02 TSVPDD177 pKa = 3.16 VGYY180 pKa = 8.78 TRR182 pKa = 11.84 TQLADD187 pKa = 3.48 LTEE190 pKa = 4.21 KK191 pKa = 10.41 TKK193 pKa = 10.33 VRR195 pKa = 11.84 QVWGRR200 pKa = 11.84 AFHH203 pKa = 6.55 YY204 pKa = 10.25 ILLEE208 pKa = 4.02 GLVAQPFIQSIMEE221 pKa = 4.36 GPSFIHH227 pKa = 6.66 TGRR230 pKa = 11.84 DD231 pKa = 3.27 PTLSVPQSLAKK242 pKa = 10.57 VSSQCKK248 pKa = 9.75 YY249 pKa = 10.34 IYY251 pKa = 10.89 SLDD254 pKa = 3.53 WKK256 pKa = 11.19 SFDD259 pKa = 3.12 ATVNRR264 pKa = 11.84 FEE266 pKa = 5.28 INTSFDD272 pKa = 3.5 IIKK275 pKa = 10.57 SKK277 pKa = 11.16 VIFPNYY283 pKa = 7.0 EE284 pKa = 3.55 TEE286 pKa = 3.98 QAFEE290 pKa = 3.82 ITRR293 pKa = 11.84 QLFLHH298 pKa = 6.4 KK299 pKa = 10.14 KK300 pKa = 8.12 VAAPDD305 pKa = 4.08 GYY307 pKa = 10.13 IYY309 pKa = 9.6 EE310 pKa = 4.17 AHH312 pKa = 6.84 KK313 pKa = 10.56 GIPSGSYY320 pKa = 6.51 YY321 pKa = 10.55 TSMVGSIVNRR331 pKa = 11.84 LRR333 pKa = 11.84 IEE335 pKa = 4.36 YY336 pKa = 9.01 IWRR339 pKa = 11.84 IATGHH344 pKa = 6.11 GPIHH348 pKa = 7.12 CEE350 pKa = 3.71 TLGDD354 pKa = 4.95 DD355 pKa = 4.45 SLCGDD360 pKa = 5.25 DD361 pKa = 4.23 IFVPATQLADD371 pKa = 2.98 IANRR375 pKa = 11.84 IGWYY379 pKa = 10.2 FNADD383 pKa = 2.91 KK384 pKa = 10.71 TEE386 pKa = 3.87 YY387 pKa = 9.31 STIPEE392 pKa = 4.2 GVTFLGRR399 pKa = 11.84 TSTGNLNSRR408 pKa = 11.84 DD409 pKa = 3.43 LTKK412 pKa = 10.61 CLRR415 pKa = 11.84 LLVYY419 pKa = 9.82 PEE421 pKa = 4.27 YY422 pKa = 9.97 PVTSGRR428 pKa = 11.84 ISAYY432 pKa = 9.12 RR433 pKa = 11.84 ARR435 pKa = 11.84 SIADD439 pKa = 3.41 DD440 pKa = 3.77 SGGLSDD446 pKa = 6.05 LLNQVAIRR454 pKa = 11.84 LEE456 pKa = 3.93 RR457 pKa = 11.84 SYY459 pKa = 11.53 GIASEE464 pKa = 4.48 EE465 pKa = 4.05 EE466 pKa = 3.45 IPAYY470 pKa = 9.91 FKK472 pKa = 10.74 RR473 pKa = 11.84 YY474 pKa = 8.94 VPFMM478 pKa = 4.76
Molecular weight: 54.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.18
IPC2_protein 6.198
IPC_protein 6.326
Toseland 6.62
ProMoST 6.664
Dawson 6.664
Bjellqvist 6.62
Wikipedia 6.678
Rodwell 6.649
Grimsley 6.766
Solomon 6.664
Lehninger 6.664
Nozaki 6.912
DTASelect 7.088
Thurlkill 7.117
EMBOSS 7.102
Sillero 7.044
Patrickios 3.961
IPC_peptide 6.678
IPC2_peptide 6.854
IPC2.peptide.svr19 6.832
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
872
394
478
436.0
49.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.569 ± 0.186
1.147 ± 0.076
6.651 ± 0.598
4.472 ± 1.044
4.243 ± 0.36
5.161 ± 0.841
1.95 ± 0.265
6.078 ± 1.096
4.014 ± 0.444
8.486 ± 0.089
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.179 ± 1.012
3.555 ± 0.157
5.734 ± 0.853
3.555 ± 0.159
6.766 ± 0.054
6.651 ± 0.821
8.257 ± 1.335
6.307 ± 0.024
1.835 ± 0.437
5.39 ± 0.51
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here