Olleya aquimaris
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2642 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A327R5Q1|A0A327R5Q1_9FLAO Outer membrane receptor protein involved in Fe transport OS=Olleya aquimaris OX=639310 GN=LY08_02422 PE=3 SV=1
DDD2 pKa = 5.84 IDDD5 pKa = 3.66 NCDDD9 pKa = 3.07 NIDDD13 pKa = 3.75 EE14 pKa = 6.35 FDDD17 pKa = 3.4 DDD19 pKa = 3.97 DDD21 pKa = 4.02 FTACQGDDD29 pKa = 4.7 DDD31 pKa = 5.45 DD32 pKa = 5.3 DDD34 pKa = 4.19 NSYYY38 pKa = 10.12 GAPEEE43 pKa = 3.83 CDDD46 pKa = 5.83 IDDD49 pKa = 3.54 NCDDD53 pKa = 3.27 LVDDD57 pKa = 4.18 DDD59 pKa = 4.23 DD60 pKa = 3.87 SVIGLPDDD68 pKa = 3.3 VITEEE73 pKa = 4.18 PLGDDD78 pKa = 3.84 CQGVAEEE85 pKa = 4.25 TVDDD89 pKa = 3.53 LNASDDD95 pKa = 4.74 SSISYYY101 pKa = 9.43 WSNGSTDDD109 pKa = 2.79 SIIVSDDD116 pKa = 3.57 NTYYY120 pKa = 8.97 VSVTGNSGCTTTSSYYY136 pKa = 10.75 STAVPSDDD144 pKa = 3.61 LSGYYY149 pKa = 10.36 MLANKKK155 pKa = 9.68 EE156 pKa = 4.13 YYY158 pKa = 10.47 KKK160 pKa = 9.62 EE161 pKa = 4.1 TVNGGGVGVINNGKKK176 pKa = 8.49 KK177 pKa = 10.55 KKK179 pKa = 10.22 RR180 pKa = 11.84 DDD182 pKa = 3.94 DD183 pKa = 3.81 SVFSFVKKK191 pKa = 10.35 DDD193 pKa = 5.02 IDDD196 pKa = 4.07 DDD198 pKa = 3.54 SSYYY202 pKa = 10.26 QQSIFDDD209 pKa = 4.16 ASVTLPTFYYY219 pKa = 11.18 YY220 pKa = 9.46 TADDD224 pKa = 4.43 DD225 pKa = 4.36 DD226 pKa = 4.57 EEE228 pKa = 4.57 DDD230 pKa = 3.62 TVPEEE235 pKa = 4.67 QTITINSDDD244 pKa = 2.92 YYY246 pKa = 11.92 DD247 pKa = 3.43 DD248 pKa = 4.37 KKK250 pKa = 10.75 KKK252 pKa = 11.04 KK253 pKa = 9.82 GTLIIDDD260 pKa = 3.6 PEEE263 pKa = 4.0 YYY265 pKa = 9.48 HHH267 pKa = 7.2 KK268 pKa = 10.41 DDD270 pKa = 4.59 EEE272 pKa = 4.94 DD273 pKa = 3.82 ATIIFNQSCSVMIDDD288 pKa = 2.96 YY289 pKa = 11.52 DD290 pKa = 3.48 DDD292 pKa = 4.16 NDDD295 pKa = 4.05 CLIDDD300 pKa = 3.83 NGYYY304 pKa = 8.85 VVFYYY309 pKa = 10.5 EEE311 pKa = 4.5 KK312 pKa = 10.43 KK313 pKa = 9.65 KKK315 pKa = 9.51 DDD317 pKa = 3.19 GTTIDDD323 pKa = 3.82 NIYYY327 pKa = 7.32 QKKK330 pKa = 9.29 EE331 pKa = 3.89 HHH333 pKa = 6.53 KKK335 pKa = 10.42 YYY337 pKa = 10.41 NNHHH341 pKa = 3.92 TMMGLFISEEE351 pKa = 4.05 YY352 pKa = 9.81 KK353 pKa = 10.21 KKK355 pKa = 10.6 EEE357 pKa = 5.25 KK358 pKa = 9.69 YY359 pKa = 8.18 DDD361 pKa = 3.87 YYY363 pKa = 10.39 STICEEE369 pKa = 4.39 IPAPSNPLPCNDDD382 pKa = 5.56 DD383 pKa = 5.41 DD384 pKa = 7.38 DD385 pKa = 7.62 DD386 pKa = 7.72 DD387 pKa = 7.58 DD388 pKa = 7.65 DD389 pKa = 7.65 DD390 pKa = 7.65 DD391 pKa = 7.64 DD392 pKa = 7.49 DD393 pKa = 7.59 DD394 pKa = 7.3 DD395 pKa = 5.79 DD396 pKa = 6.17 DD397 pKa = 6.2 VGQVNTIDDD406 pKa = 4.87 YYY408 pKa = 10.35 VPAKKK413 pKa = 10.24 EEE415 pKa = 4.12 NINLVSQTHHH425 pKa = 5.81 KKK427 pKa = 9.96 KKK429 pKa = 10.55 YY430 pKa = 9.42 ISNIRR435 pKa = 11.84 GTTVISKKK443 pKa = 9.43 EE444 pKa = 4.14 IVTKKK449 pKa = 10.53 NNSLSVDDD457 pKa = 3.5 TKKK460 pKa = 10.87 PEEE463 pKa = 4.01 EEE465 pKa = 4.45 YY466 pKa = 10.3 FTIVLDDD473 pKa = 4.06 NRR475 pKa = 11.84 LQKKK479 pKa = 10.92 KK480 pKa = 9.54 IKKK483 pKa = 10.28 SN
Molecular weight: 53.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.675
IPC2_protein 3.77
IPC_protein 3.834
Toseland 3.592
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.808
Rodwell 3.656
Grimsley 3.49
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.253
Thurlkill 3.656
EMBOSS 3.808
Sillero 3.961
Patrickios 1.494
IPC_peptide 3.834
IPC2_peptide 3.935
IPC2.peptide.svr19 3.84
Protein with the highest isoelectric point:
>tr|A0A327RL91|A0A327RL91_9FLAO Glycine/D-amino acid oxidase-like deaminating enzyme OS=Olleya aquimaris OX=639310 GN=LY08_00048 PE=4 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.06 RR4 pKa = 11.84 TFQPSKK10 pKa = 9.13 RR11 pKa = 11.84 KK12 pKa = 9.48 RR13 pKa = 11.84 RR14 pKa = 11.84 NKK16 pKa = 9.49 HH17 pKa = 3.94 GFRR20 pKa = 11.84 EE21 pKa = 4.27 RR22 pKa = 11.84 MASANGRR29 pKa = 11.84 KK30 pKa = 9.04 VLARR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.09 GRR40 pKa = 11.84 KK41 pKa = 8.0 KK42 pKa = 10.66 LSVSTEE48 pKa = 3.92 TRR50 pKa = 11.84 HH51 pKa = 6.44 KK52 pKa = 10.57 KK53 pKa = 9.81
Molecular weight: 6.35 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 10.76
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.296
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 12.018
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2642
0
2642
899899
27
2399
340.6
38.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.296 ± 0.048
0.752 ± 0.015
5.955 ± 0.037
6.222 ± 0.05
5.229 ± 0.039
6.16 ± 0.048
1.734 ± 0.021
7.927 ± 0.042
7.782 ± 0.063
9.257 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.007 ± 0.023
6.691 ± 0.055
3.296 ± 0.029
3.663 ± 0.029
3.076 ± 0.029
6.294 ± 0.04
6.339 ± 0.05
6.278 ± 0.035
0.97 ± 0.015
4.072 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here